SSGCID: Target Page

BrmeA.18208.a

ATP-dependent DNA helicase

CENTER ID:	BrmeA.18208.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18208.a.A2.GE33797	domain1		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.301
RefSeq:	NP_539193.1
UniProt:	Q8YJ11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQQPAHDLP LTLSGRDVTA VLGPTNTGKT HLAIERMLSH GSGMIGLPLR LLAREVYNRV AERVGAANVS LVTGEEKIVP PGARYSVCTV EAMPRHTDAA FVAIDEVQLA NDLERGHIFT DRILHLRGRQ ETLLLGAATM RGILEKLLRG VNVVTRPRLS HLAYAGSKKM TRLPNRSAIV AFSADEVYAI AELIRRQRGG AAVVMGALSP RTRNAQVELY QSGDVDFLVA TDAIGMGLNL DVDHVAFAQN RKFDGYQFRD LTPAEVGQIA GRAGRHVRDG TFGVTGQVHP FDEELVERVE AHNFDPVKVL QWRTSRFDFS SVEALLRSLE TPAPIEGLAK ALPAVDQQAL ENLSKDDEIM RLATTPSRIE LLWDACALPD YRKIAPAQHA DIIATIYQDL VRRGSVDEDY MSEQVRRADS TEGEIDTLSH RVAQIRTWTF VSHRPGWLAD PTHWQEKTRE IEDRLSDALH ERLTKRFVDR RTSVLMRRLR ENAMLEAEIS PAGDVIVEGH NVGQLEGFRF TADVQAEGPD AKAVKTAAQK ALAAEFERRA ERFAAAPNGD LALGSDGVLR WIGATVATLV AGDDVLKPRT VILADEQLTG PARDKVDARI DRFVAHHIET VLKPLADLAN ADALTGMTRG LAFQIVENLG ILQRRDVAEE VRGLDQDSRA ALRRLGVRFG AYHVFMPMLL KPAPAGLITL LWALKNDGRE RAGYGDVVNV LAAGRTSVVV DPAFDPMFYR LAGYRVLGRR AVRIDILERL ADLIRPALAW RPGSGARPDG AYDGGRFIVT PAMMSILGAT TEDMEEILKS LGYRSEAMEV AAVAAKLAEL DAFAAEAGGK DAAAPVEAEK PAVPPAEPVP APVVAAEPEA KVEETAAPAI EAAPVAEASA GEAEEPKPVL LWRQGRFEGR NRNQDQNRQR QNRGNQHRGN QNRGHQNREE GGAARKAEGG EEQNPRGGNK RFDKHKRQHD GEGKREGAKG EFRGGNQRQP KRDHVYQGNK RTEQERPVRI DPDSPFAKLL ALKEQMKK

NT Sequence

atgaaccagc aacctgccca tgatctgccg ctgacattga gtggccgcga cgtgaccgcg gtgctggggc cgaccaatac cggcaagaca cacctggcca tcgagcggat gctgtcgcat gggagcggca tgatcggcct gccgctgcgc ctgcttgcgc gcgaggttta taatcgcgtg gccgagcggg taggggcagc caatgtttcg ctggtaacgg gcgaggaaaa gatcgtgccg ccgggtgcgc gctattccgt ctgtacggtg gaggccatgc cgcgccacac agatgcggcc ttcgtcgcca ttgatgaagt gcaactggcc aacgatctgg agcgcggcca tattttcacc gaccgtatcc tgcatctgcg cggacggcag gaaacgctgc tgcttggtgc ggccaccatg cgcggcattc tggaaaagct gctgcgcggc gtgaatgtgg tgacacgtcc gcgtctttcg catctggcct atgccggatc gaagaagatg acgcgcctgc ccaaccgttc cgcaatcgtg gccttttccg ccgacgaggt ttacgccatc gccgagctta tccgccgcca gcgcggcggg gcggcggtgg tgatgggtgc gctcagcccg cgtacgcgca atgcgcaggt ggagctttac cagtcgggcg atgtcgattt tctggtggcg acggatgcga tcggcatggg gttgaacctc gatgtggatc acgtcgcctt tgcgcagaac cgcaagttcg acggctatca gttccgtgat ctgacgcccg ccgaagtggg gcagattgcg ggccgtgcgg gccgccatgt gcgcgatggc acttttggcg tgaccggtca ggtccatcct tttgacgagg aactggttga gcgggtggaa gcgcataatt tcgatccggt gaaggtgctg caatggcgca cgtcccgttt cgacttttcc tctgtcgagg cgctgcttcg ttcgctggaa acgcctgcgc ccattgaggg gcttgccaag gctctgcctg ccgtcgatca acaggcgctt gaaaatctgt cgaaagacga cgagattatg cggcttgcaa cgacgccgtc gcgcatcgag cttttgtggg atgcgtgcgc gctgcctgac tatcgcaaga tcgcaccggc acagcatgcc gatattattg caaccatcta tcaggatctt gtacgccgag ggagcgttga tgaagattat atgagcgaac aggtgcgccg cgccgacagc acagagggag aaatcgacac cctgtcgcat cgcgtggccc aaatccgtac ctggactttc gtgtcgcaca ggcctggatg gcttgccgat ccgacacact ggcaggaaaa gacgcgcgaa atagaggaca gactgtccga tgcgctgcat gagaggttga cgaaacgctt tgtagaccgc aggacaagcg tgcttatgag gcgcctgaga gagaatgcca tgcttgaagc agaaatcagc cctgccggag acgtgatcgt cgagggccat aatgtcgggc aactggaagg attccgtttc actgccgacg tccaggccga ggggccggac gccaaagctg tgaagacggc tgcacaaaag gcgctggccg ccgagtttga acgccgagcg gagcgctttg ctgctgcgcc aaatggcgat ctcgcgcttg gttcggatgg cgtcctgcgc tggatcggcg cgaccgtcgc cacgctggtt gcgggcgatg acgtgttgaa gccgcgcacg gtcattctgg ccgacgaaca actgacgggg ccagcgcgcg acaaggtcga cgcccgtatt gaccgtttcg ttgcccatca tatcgaaacc gttctgaagc cgcttgccga tctggcaaat gcggatgcgc tgacgggcat gacgcgcggc ctggctttcc agatcgtcga aaacctgggc attctccagc gccgtgacgt ggcggaggaa gtgcgcggcc ttgatcagga ttcgcgtgcg gcgcttcgcc gtctgggcgt gcgtttcggt gcttatcacg ttttcatgcc catgctgcta aagccggccc cggctggcct catcacgctt ctctgggcgc tcaagaacga tggccgcgaa cgggcaggtt acggcgatgt cgtcaatgtg ctggccgctg gccgcacctc ggtcgtggtt gatccggcct tcgatccgat gttctaccgt ctcgctggct atcgtgtgct gggccggcgc gcggttcgca tcgatattct ggagcgcctt gccgatctca ttcgcccggc tcttgcctgg cgtccgggtt ccggcgctcg cccggatggc gcttatgatg gtggacgctt catcgtgacg cctgccatga tgtccattct gggggcgaca accgaggata tggaagaaat cctcaagagc cttggctatc gcagtgaggc aatggaagtc gctgcggtgg cggcgaagct tgcggagctt gatgcattcg cagccgaggc tggtggcaag gatgcagccg cgccggttga agctgaaaag ccagccgtgc cgccggcaga acctgttccc gcgccggtcg tggctgctga acccgaggct aaagtggaag agacggcggc tccagccatc gaagctgctc ctgttgctga agcgtctgcg ggcgaggcgg aagagccgaa gccggttctt ctgtggcgtc agggccgttt tgaaggccgc aaccgcaatc aggaccagaa tcgccagcgc cagaatcgtg gaaaccagca tcgtggcaac cagaatcgcg gccatcagaa ccgcgaagag ggtggtgcgg cacgcaaggc tgagggtggt gaagaacaga atccgcgtgg cggcaacaag cgctttgata agcacaagcg ccaacacgac ggcgaaggca agcgcgaagg cgcgaagggc gaattccgtg gtggcaatca gcgccagcca aagcgcgatc atgtctatca gggcaacaag cggacggaac aggagcggcc tgttcgtatc gaccccgatt cgcctttcgc caagcttctg gcgctcaagg aacagatgaa gaagtaa

Details for BrmeA.18208.a.A2.GE33797

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNQQPAHDL PLTLSGRDVT AVLGPTNTGK THLAIERMLS HGSGMIGLPL RLLAREVYNR VAERVGAANV SLVTGEEKIV PPGARYSVCT VEAMPRHTDA AFVAIDEVQL ANDLERGHIF TDRILHLRGR QETLLLGAAT MRGILEKLLR GVNVVTRPRL SHLAYAGSKK MTRLPNRSAI VAFSADEVYA IAELIRRQRG GAAVVMGALS PRTRNAXXXF TSRAMSIFWX XXXXSXWG

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacc agcaacctgc ccatgatctg ccgctgacat tgagtggccg cgacgtgacc gcggtgctgg ggccgaccaa taccggcaag acacacctgg ccatcgagcg gatgctgtcg catgggagcg gcatgatcgg cctgccgctg cgcctgcttg cgcgcgaggt ttataatcgt gtggccgagc gggtaggggc agccaatgtt tcgctggtaa cgggcgagga aaagatcgtg ccgccgggtg cgcgctattc cgtctgtacg gtggaggcca tgccgcgcca cacagatgcg gccttcgtcg ccattgatga agtgcaactg gccaacgatc tggagcgcgg ccatattttc accgaccgta tcctgcatct gcgcggacgg caggaaacgc tgctgcttgg tgcggccacc atgcgcggca ttctggaaaa gctgctgcgc ggcgtgaatg tggtgacacg tccgcgtctt tcgcatctgg cctatgccgg atcgaagaag atgacgcgcc tgcccaaccg ttccgcaatc gtggcctttt ccgccgacga ggtttacgcc atcgccgagc ttatccgccg ccagcgcggc ggggcggcng tggtgatggg tgcgctcagc ccgcgtacgc gcaatgcgcn nnggnncttt accagtcggg cgatgtcgat tttctggngg cnanggangc natcggnatg gggttgaann tcnatgnngn tcacgtcttg nncanaannn cnngttcnan gnnntcantn cnngatcnga cnnccngncn nnnggnnnna nnnnnnnngc gnnnnnnnnn cncnnngnnn nncngncnnn nnttnnnnnn nnnnnnnnnn nnnnnnnann nnagnnnnnn nnnngnnnnn ncnnntnnnn nnnnnnnnnn nnnnnnnnnn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagcaa cctgcccatg atctgccgct gacattgagt ggccgcgacg tgaccgcggt gctggggccg accaataccg gcaagacaca cctggccatc gagcggatgc tgtcgcatgg gagcggcatg atcggcctgc cgctgcgcct gcttgcgcgc gaggtttata atcgcgtggc cgagcgggta ggggcagcca atgtttcgct ggtaacgggc gaggaaaaga tcgtgccgcc gggtgcgcgc tattccgtct gtacggtgga ggccatgccg cgccacacag atgcggcctt cgtcgccatt gatgaagtgc aactggccaa cgatctggag cgcggccata ttttcaccga ccgtatcctg catctgcgcg gacggcagga aacgctgctg cttggtgcgg ccaccatgcg cggcattctg gaaaagctgc tgcgcggcgt gaatgtggtg acacgtccgc gtctttcgca tctggcctat gccggatcga agaagatgac gcgcctgccc aaccgttccg caatcgtggc cttttccgcc gacgaggttt acgccatcgc cgagcttatc cgccgccagc gcggcggggc ggcggtggtg atgggtgcgc tcagcccgcg tacgcgcaat gcgcaggtgg agctttacca gtcgggcgat gtcgattttc tggtggcgac ggatgcgatc ggcatggggt tgaacctcga tgtggatcac gtcgcctttg cgcagaaccg caagttcgac ggctatcagt tccgtgatct gacgcccgcc gaagtggggc agattgcggg ccgtgcgggc cgccatgtgc gcgatggcac ttttggcgtg accggtcagg tccatccttt tgacgaggaa ctggttgagc gggtggaagc gcataatttc gatccggtga aggtgctgca atggcgcacg tcccgtttcg acttttcctc tgtcgaggcg ctgcttcgtt cgctggaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a