SSGCID: Target Page

BrmeA.18202.a

Outer membrane lipoprotein omp19 (18 kDa immunoreactive antigen) (19 kDa OMP) (Minor outer membrane protein omp19)

CENTER ID:	BrmeA.18202.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18202.a.A1.GE33768	full length		1	177

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.141
RefSeq:	NP_539053.1
UniProt:	P0A3P1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGISKASLLS LAAAGIVLAG CQSSRLGNLD NVSPPPPPAP VNAVPAGTVQ KGNLDSPTQF PNAPSTDMSA QSGTQVASLP PASAPDLTPG AVAGVWNASL GGQSCKIATP QTKYGQGYRA GPLRCPGELA NLASWAVNGK QLVLYDANGG TVASLYSSGQ GRFDGQTTGG QAVTLSR

NT Sequence

atgggaattt caaaagcaag tctgctcagc ctcgcggcgg ctggcattgt cctggccggg tgccagagct cccggcttgg taatctcgat aatgtttcgc ctccgccgcc gcctgcaccg gtcaatgctg ttccggcagg cacggtgcag aaaggcaatc ttgattctcc cacacaattc cccaatgcgc cctccacgga tatgagcgcg caatccggca cacaggtcgc aagcctgccg cctgcatccg caccggacct gacgcccggc gccgtggctg gcgtctggaa cgcctcgctt ggtggtcaga gctgcaagat cgcgacgccg cagaccaaat atggccaggg ctatcgcgca ggcccgctgc gctgccccgg tgaactggct aatcttgcct cctgggccgt caatggcaag caactcgtcc tttacgatgc gaacggcggt acggttgcct cgctctattc ttcaggacag ggccgcttcg atggccagac caccggcggg caggccgtga cgctgtcgcg ctga

Details for BrmeA.18202.a.A1.GE33768

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGISKASLL SLAAAGIVLA GCQSSRLGNL DNVSPPPPPA PVNAVPAGTV QKGNLDSPTQ FPNAPSTDMS AQSGTQVASL PPASAPDLTP GAVAGVWNAS LGGQSCKIAT PQTKYGQGYR AGPLRCPGEL ANLASWAVNG KQLVLYDANG GTVASLYSSG QGRFDGQTTG GQAVTLSR

Validated NT Sequence

ttttntttnc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggaat ttcaaaagca agtctgctca gcctcgcggc ggctggcatt gtcctggccg ggtgccagag ctcccggctt ggtaatctcg ataatgtttc gcctccgccg ccgcctgcac cggtcaatgc tgttccggca ggcacggtgc agaaaggcaa tcttgattct cccacacaat tccccaatgc gccctccacg gatatgagcg cgcaatccgg cacacaggtc gcaagcctgc cgcctgcatc cgcaccggac ctgacgcccg gcgccgtggc tggcgtctgg aacgcctcgc ttggtggtca gagctgcaag atcgcgacgc cgcagaccaa atatggccag ggctatcgcg caggcccgct gcgctgcccc ggtgaactgg ctaatcttgc ctcctgggcc gtcaatggca agcaactcgt cctttacgat gcgaacggcg gtacggttgc ctcgctctat tcttcaggac agggccgctt cgatggccag accaccggcg ggcaggccgt gacgctgtcg cgctaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccnnnnnnn nnnncnngnn gggncnntna nntnggntnc nngcnnancc nntntgnnnn nnnnnntgnt gnnncnnnnn nnnnttnnnn nnnntgngnc nnnncnnnnn ngnccgcnnn ncnanannnn nnncnnnnnn ccnnnnnntc ctntgncngn nnnnctnnnn cnnnnnntnn cnanntctnn nnnnnnnnnn nnnnnnnnnn nnnnnntnnn nttnnnnnnn taccnnncat ntnttgnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaatttca aaagcaagtc tgctcagcct cgcggcggct ggcattgtcc tggccgggtg ccagagctcc cggcttggta atctcgataa tgtttcgcct ccgccgccgc ctgcaccggt caatgctgtt ccggcaggca cggtgcagaa aggcaatctt gattctccca cacaattccc caatgcgccc tccacggata tgagcgcgca atccggcaca caggtcgcaa gcctgccgcc tgcatccgca ccggacctga cgcccggcgc cgtggctggc gtctggaacg cctcgcttgg tggtcagagc tgcaagatcg cgacgccgca gaccaaatat ggccagggct atcgcgcagg cccgctgcgc tgccccggtg aactggctaa tcttgcctcc tgggccgtca atggcaagca actcgtcctt tacgatgcga acggcggtac ggttgcctcg ctctattctt caggacaggg ccgcttcgat ggccagacca ccggcgggca ggccgtgacg ctgtcgcgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa