SSGCID: Target Page

BrmeA.18201.a

Homoprotocatechuate 2,3-dioxygenase

CENTER ID:	BrmeA.18201.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18201.a.A1.GE33776	full length		1	331

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.2417
RefSeq:	NP_541113.1
UniProt:	Q8YDN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRKIMPLPT PNLHPPFNIV RLSHVELGVT DLTKSRAFYV DTLGLQVTDE SADAIYLRAL EERGHHCIVL RKTDKAEARD LGFKLYSEDD FDRAEHFFKG KGLPVEWVER PYQSRTLRTR DPHGIPLEFY SRMDRLPPIH QKYALYKGVK PLRIDHFNCF SPNVDESVAF YNELGFRVTE YTEDAETGRL WAAWTHRKGG VHDITFTNGK GPRLHHTAFW VPTPLNIIDL LDLMSTTGWL ANIERGPGRH GISNAFFLYV RDPDGHRIEI YCSDYQTVDP DLEPIKWDLK DPQRQTLWGA PAPKSWFEEG SLFASVQPVD SVLKAQPIVA P

NT Sequence

ttgaggagga aaatcatgcc cttgccgacc cctaaccttc acccaccatt caacattgtg cgcctgtcgc atgtggagct tggggtgaca gaccttacca agtcgcgggc tttctatgtt gatacgcttg gtctgcaagt gaccgacgaa agcgcagatg ccatttatct gcgcgccctg gaagagcgcg ggcatcattg catcgttttg cgcaagaccg acaaggcgga agcccgcgat cttggcttca agctctattc ggaggacgat ttcgaccggg ccgaacattt cttcaagggc aagggcctgc ctgtcgaatg ggtggagcga ccctaccagt cgcgcacgct gcgcacgcgt gatccgcacg gcattccgct tgagttctat tccagaatgg accgcctgcc gccgatccat caaaaatatg cgctctacaa gggcgtgaag ccgctgcgca tcgaccactt caactgcttc tcgcctaatg tggatgaatc cgtcgccttc tataacgagc tgggtttccg cgtgacggaa tataccgagg atgccgaaac tggccgcctg tgggccgcct ggacgcaccg caagggcggc gttcacgata tcaccttcac caatggcaag gggccgcgcc tgcaccatac cgctttctgg gtgccgacac cgctcaacat catcgacctg ctcgacctca tgtccaccac cggctggctt gccaatatcg agcgcggtcc cggccgccac ggcatttcca atgccttctt cctctatgtg cgcgacccgg atggccatcg catcgaaatc tactgctcgg attaccagac ggtcgatccc gatctggagc cgatcaagtg ggatctgaaa gacccgcagc gccagacgct ctggggtgcg cctgcaccga aatcctggtt cgaggaaggc agcttgtttg ccagtgtgca gccggtcgat tccgtgttga aggcacagcc gatcgtcgcg ccgtaa

Details for BrmeA.18201.a.A1.GE33776

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	80
PERCENT COVERAGE:	103

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLRRKIMPL PTPNLHPPFN IVRLSHVELG VTDLTKSRAF YVDTLGLQVT DESADAIYLR ALEERGHHCI VLRKTDKAEA RDLGFKLYSE DDFDRAEHFF KGKGLPVEWV ERPYQSRTLR TRDPHGIPLE FYSRMDRLPP IHQKYALYKG VKPLRIDHFN CFSPNVDESV AFYNELGFRV TEYTEDAETG RLWAAWTHRX GGVHDITFTN GKGPRLHHTA FWVPTPLNII DLLDLMSTTG WLXNIERGPG RHGISXXXFX YVRDPXXXIA SXSTXXITXX RSXSXPXXXX XXTRXXXXXG XXXXXXXXXX LXAXXXXXXX XXXXXXXXXX XXXSXXXXXX XXXXXX

Validated NT Sequence

ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgag gaggaaaatc atgcccttgc cgacccctaa ccttcaccca ccattcaaca ttgtgcgcct gtcgcatgtg gagcttgggg tgacagacct taccaagtcg cgggctttct atgttgatac gcttggtctg caagtgaccg acgaaagcgc agatgccatt tatctgcgcg ccctggaaga gcgcgggcat cattgcatcg ttttgcgcaa gaccgacaag gcggaagccc gcgatcttgg cttcaagctc tattcggagg acgatttcga ccgggccgaa catttcttca agggcaaggg cctgcctgtc gaatgggtgg agcgacccta ccagtcgcgc acgctgcgca cgcgtgatcc gcacggcatt ccgcttgagt tctattccag aatggaccgc ctgccgccga tccatcaaaa atatgcgctc tacaagggcg tgaagccgct gcgcatcgac cacttcaact gcttctcgcc taatgtggat gaatccgtcg ccttctataa cgagctgggt ttccgcgtga cggaatatac cgaggatgcc gaaactggcc gcctgtgggc cgcctggacg caccgcangg gcggcgttca cgatatcacc ttcaccaatg gcaaggggcc gcgcctgcac cataccgctt tctgggtgcc gacaccgctc aacatcatcg acctgctcga cctcatgtcc accaccggct ggcttgncaa tatcgagcgc ggtcccggcc gccacggcat ttccnatgnc ntcttccnct atgtgcgcga cccngnatng nccatcgcat cgaantctac tgntcngatt accananggc gatcccnatc nnnnccnatn annngnnnct gnannacccg cnnnnnnnan nnnnctgggg ngnncngnnc nnanncnnnn nnnnnnnagc tnnntgcngn ncannnncnn tnnnnnnnnn nnnnnannnn nnnncnnnnt nnnnnnnnnn tnnnnnnntc ngnnnnnnnn nnnnnnnnnn nnnnannnnn nnnntnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLRRKIMPL PTPNLHPPFN IVRLSHVELG VTDLTKSRAF YVDTLGLQVT DESADAIYLR ALEERGHHCI VLRKTDKAEA RDLGFKLYSE DDFDRAEHFF KGKGLPVEWV ERPYQSRTLR TRDPHGIPLE FYSRMDRLPP IHQKYALYKG VKPLRIDHFN CFSPNVDESV AFYNELGFRV TEYTEDAETG RLWAAWTHRK GGVHDITFTN GKGPRLHHTA FWVPTPLNII DLLDLMSTTG WLANIERGPG RHGISNAFFL YVRDPDGHRI EIYCSDYQTV DPDLEPIKWD LKDPQRQTLW GAPAPKSWFE EGSLFASVQP VDSVLKAQPI VAP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaggagg aaaatcatgc ccttgccgac ccctaacctt cacccaccat tcaacattgt gcgcctgtcg catgtggagc ttggggtgac agaccttacc aagtcgcggg ctttctatgt tgatacgctt ggtctgcaag tgaccgacga aagcgcagat gccatttatc tgcgcgccct ggaagagcgc gggcatcatt gcatcgtttt gcgcaagacc gacaaggcgg aagcccgcga tcttggcttc aagctctatt cggaggacga tttcgaccgg gccgaacatt tcttcaaggg caagggcctg cctgtcgaat gggtggagcg accctaccag tcgcgcacgc tgcgcacgcg tgatccgcac ggcattccgc ttgagttcta ttccagaatg gaccgcctgc cgccgatcca tcaaaaatat gcgctctaca agggcgtgaa gccgctgcgc atcgaccact tcaactgctt ctcgcctaat gtggatgaat ccgtcgcctt ctataacgag ctgggtttcc gcgtgacgga atataccgag gatgccgaaa ctggccgcct gtgggccgcc tggacgcacc gcaagggcgg cgttcacgat atcaccttca ccaatggcaa ggggccgcgc ctgcaccata ccgctttctg ggtgccgaca ccgctcaaca tcatcgacct gctcgacctc atgtccacca ccggctggct tgccaatatc gagcgcggtc ccggccgcca cggcatttcc aatgccttct tcctctatgt gcgcgacccg gatggccatc gcatcgaaat ctactgctcg gattaccaga cggtcgatcc cgatctggag ccgatcaagt gggatctgaa agacccgcag cgccagacgc tctggggtgc gcctgcaccg aaatcctggt tcgaggaagg cagcttgttt gccagtgtgc agccggtcga ttccgtgttg aaggcacagc cgatcgtcgc gccgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa