SSGCID: Target Page

BrmeA.18198.a

Glycerol-3-phosphate regulon repressor

CENTER ID:	BrmeA.18198.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18198.a.A1.GE33775	full length		1	263

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.3455
RefSeq:	NP_542071.1
UniProt:	Q8YB08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIPAERQALI VSRLSARGIL SIAELAEMLG VSPMTVRRDI HSLEEQGRAL SVAGGVQLPE RIFSEPSHIV KAALYHNEKA AIGQLAAQMI RPGTVIYLDA GTTTLEIAYR LTDRQDLTVV TNDFVIAAFL SQESNCKLFH TGGEVERENQ SCVGSAAGEA IRRFNFDLAF ISTSSFGARG VSTPSENKIA VKRAVVESAV RRVLVTDSSK YGLIGTFNAI PLDVLSAVVT DAGLPENARE TITQRGVELH LVEAGTGKVE GKI

NT Sequence

atgataccgg ctgaacggca ggctctgatt gtcagccgcc tttcggcgcg cggcatcttg agcattgcgg aactggccga aatgctgggc gtatcgccaa tgacggtgcg ccgcgatatt cattcgctgg aagaacaggg gcgtgcgctt tccgtggctg gcggcgtaca gcttcctgaa cggattttca gcgagccttc ccatatcgtc aaggcggccc tttaccacaa tgagaaagcg gcgatcggtc agcttgcggc acagatgata agacccggaa cggtgatcta tctcgatgcg ggtacaacca cgcttgaaat agcctaccgg ctcacagacc ggcaggacct cacggtcgtc accaatgatt tcgtgattgc cgcgttcctt tcgcaggaga gcaattgcaa gcttttccat acgggcggcg aagtggaacg agaaaaccaa tcctgcgtgg gaagtgccgc cggggaggcc atccgtcgtt tcaacttcga tctcgcattc atctcgacat cctcattcgg cgcgcgtggt gtgtccacgc cctccgaaaa caagatcgcc gtaaaacgcg ccgtcgttga aagtgcggtt cgcagggtgc ttgtaacaga cagcagcaaa tacggcctca tcggcacatt caatgcaatc ccgctcgacg tgttgtccgc cgtcgttacc gatgcgggcc tgcccgaaaa tgcgcgggaa acaatcacgc agcgcggcgt cgagttgcat ctggttgaag ccggcacagg caaagtcgaa gggaaaatct ga

Details for BrmeA.18198.a.A1.GE33775

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMIPAERQ ALIVSRLSAR GILSIAELAE MLGVSPMTVR RDIHSLEEQG RALSVAGGVQ LPERIFSEPS HIVKAALYHN EKAAIGQLAA QMIRPGTVIY LDAGTTTLEI AYRLTDRQDL TVVTNDFVIA AFLSQESNCK LFHTGGEVER ENQSCVGSAA GEAIRRFNFD LAFISTSSFG ARGVSTPSEN KIAVKRAVVE SAVRRVLVTD SSKYGLIGTF NAIPLDVLSA VVTDAGLPEN ARETITQRGV ELHLVEAGTG KVEG

Validated NT Sequence

tcccttcgac tttgcctgtg ccggcttcaa ccagatgcaa ctcgacgccg cgctgcgtga ttgtttcccg cgcattttcg ggcaggcccg catcggtaac gacggcggac aacacgtcga gcgggattgc attgaatgtg ccgatgaggc cgtatttgct gctgtctgtt acaagcaccc tgcgaaccgc actttcaacg acggcgcgtt ttacggcgat cttgttttcg gagggcgtgg acacaccacg cgcgccgaat gaggatgtcg agatgaatgc gagatcgaag ttgaaacgac ggatggcctc cccggcggca cttcccacgc aggattggtt ttctcgttcc acttcgccgc ccgtatggaa aagcttgcaa ttgctctcct gcgaaaggaa cgcggcaatc acgaaatcat tggtgacgac cgtgaggtcc tgccggtctg tgagccggta ggctatttca agcgtggttg tacccgcatc gagatagatc accgttccgg gtcttatcat ctgtgccgca agctgaccga tcgccgcttt ctcattgtgg taaagggccg ccttgacgat atgggaaggc tcgctgaaaa tccgttcagg aagctgtacg ccgccagcca cggaaagcgc acgcccctgt tcttccagcg aatgaatatc gcggcgcacc gtcattggcg atacgcccag catttcggcc agttccgcaa tgctcaagat gccgcgcgcc gaaaggcggc tgacaatcag agcctgccgt tcagccggta tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIPAERQAL IVSRLSARGI LSIAELAEML GVSPMTVRRD IHSLEEQGRA LSVAGGVQLP ERIFSEPSHI VKAALYHNEK AAIGQLAAQM IRPGTVIYLD AGTTTLEIAY RLTDRQDLTV VTNDFVIAAF LSQESNCKLF HTGGEVEREN QSCVGSAAGE AIRRFNFDLA FISTSSFGAR GVSTPSENKI AVKRAVVESA VRRVLVTDSS KYGLIGTFNA IPLDVLSAVV TDAGLPENAR ETITQRGVEL HLVEAGTGKV EGKI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataccggct gaacggcagg ctctgattgt cagccgcctt tcggcgcgcg gcatcttgag cattgcggaa ctggccgaaa tgctgggcgt atcgccaatg acggtgcgcc gcgatattca ttcgctggaa gaacaggggc gtgcgctttc cgtggctggc ggcgtacagc ttcctgaacg gattttcagc gagccttccc atatcgtcaa ggcggccctt taccacaatg agaaagcggc gatcggtcag cttgcggcac agatgataag acccggaacg gtgatctatc tcgatgcggg tacaaccacg cttgaaatag cctaccggct cacagaccgg caggacctca cggtcgtcac caatgatttc gtgattgccg cgttcctttc gcaggagagc aattgcaagc ttttccatac gggcggcgaa gtggaacgag aaaaccaatc ctgcgtggga agtgccgccg gggaggccat ccgtcgtttc aacttcgatc tcgcattcat ctcgacatcc tcattcggcg cgcgtggtgt gtccacgccc tccgaaaaca agatcgccgt aaaacgcgcc gtcgttgaaa gtgcggttcg cagggtgctt gtaacagaca gcagcaaata cggcctcatc ggcacattca atgcaatccc gctcgacgtg ttgtccgccg tcgttaccga tgcgggcctg cccgaaaatg cgcgggaaac aatcacgcag cgcggcgtcg agttgcatct ggttgaagcc ggcacaggca aagtcgaagg gaaaatctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa