BrmeA.18188.a

Cobyrinic acid A,C-diamide synthase

CENTER ID: BrmeA.18188.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18188.a.A1.GE33771 full length 1 436
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.788
RefSeq: NP_539622.1
UniProt: Q8YHU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGFMIAAPA SGSGKTTVTL GLLRALKRRG EVLAPVKAGP DYIDPAYHRA ASGVDCFNLD PWAMRPELIS ALSSRMTESG ARVLVAEGMM GLFDGAIDGK GSSADLARLL DLPVVLVVDC ARQSHSIAAL VWGFSQFRKD VLIEGVILNR VGSPRHEAML RGALAPLGVP VLGALPRDPA LSLPERHLGL VQADEHAGLE SFLEQAADVM EAHIDMDALQ TIWLRPKRYD AMANVARLKP LGNRIAVARD DAFAFAYMHL FEGWRRRGAE ISFFSPLADE APKADADAIY LPGGYPELHA QRLAGASRFR TAIGDAAARG VTVYGECGGY MVLGKTLEDA AGVHHPMLGL LPLETSFARR KLHLGYRLLE PLGGLPWDMP LKAHEFHYAS IVREEKADRL FRVRDASGEN LGEAGLRVGS VSGSFMHVID FSGEAA
NT Sequence
atgaagggat tcatgattgc cgcaccggcc tccgggtcgg gcaagacgac cgtgaccttg ggtttgttgc gcgccctgaa acgcaggggc gaggtgcttg cgccggtgaa ggccgggccg gattatatcg accccgccta tcacagggcg gcgagtggcg tggattgttt caatctcgac ccctgggcca tgcgaccgga actcatcagt gcgctttcct cccgcatgac ggaaagcggc gcgcgtgttc ttgtcgccga aggcatgatg gggcttttcg acggggcgat tgacggtaag ggctcttctg ccgatctggc gcggcttctg gatttgccag tcgtgctggt ggtggattgc gcccggcaat cccactccat cgcggcgctg gtgtggggtt tcagccagtt tcgcaaggat gtgctgattg agggtgtcat tctcaatcgt gtcggaagtc cccgccatga agccatgctg cggggcgctc tggcacccct tggcgtcccg gttctgggtg cgctgccgcg agatccggca ctttccttgc cggaaaggca tctggggctg gtgcaggcgg acgaacatgc cggtcttgaa agctttctcg aacaagcggc cgatgtgatg gaagcgcata tcgacatgga tgcgcttcag accatctggc tacgcccgaa gcgctatgac gccatggcca atgtcgcgcg tctgaaaccg cttggaaacc gcattgccgt ggcgcgggac gatgcctttg cttttgccta tatgcatctt tttgaaggct ggcgcaggcg gggtgcggaa atctctttct tttccccgct ggcagatgaa gcaccgaagg cagatgcgga tgcaatctat cttcccggtg gttatccaga acttcatgcg caacgattgg ccggtgcatc ccgtttccgc acggcgatag gggacgctgc cgcgcgtggc gtcacggtct atggcgagtg cggcggctat atggtcctcg ggaaaacact ggaggatgcg gcaggcgttc accatcccat gctggggctt ttgccattgg aaacgagctt tgccaggcgg aaactgcatc ttggctatcg cctgctggag ccgcttggcg gattgccgtg ggacatgcct ttgaaagcgc atgaattcca ttatgcaagc atcgtgcggg aagaaaaggc cgaccgcctg ttccgggtgc gcgatgcatc gggcgagaac cttggcgaag cgggcttgcg ggttggttcc gtctccggct ccttcatgca tgtcattgat tttagcggag aagcggcgtg a
Details for BrmeA.18188.a.A1.GE33771
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/11/2011
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 83
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGFMIAAP ASGSGKTTVT LGLLRALKRR GEVLAPVKAG PDYIDPAYHR AASGVDCFNL DPWAMRPELI SALSSRMTES GARVLVAEGM MGLFDGAIDG KGSSADLARL LDLPVVLVVD CARQSHSIAA LVWGFSQFRK DVLIEGVILN RVGSPRHEAM LRGALAPLGV PVLGALPRDP ALSLPERHLG LVQADEHAGL ESFLEQAADV MEAHIDMDAL QTIWLRPKRY DAMANVARLK PLGNRIAVAR DDAFAFAYMH LFXGWRXGVR KSLSFPRWQM KHRXADAXAI YLPXGXPELH AQRXXXASXX RTXXGXAXAX XXXXYGXXXX XXXXXXXXXX XXXXPXGXXX XXXXXXXXXX XXX
Validated NT Sequence
tattttgnnn nnnttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaag ggattcatga ttgccgcacc ggcctccggg tcgggcaaga cgaccgtgac cttgggtttg ttgcgcgccc tgaaacgcag gggcgaggtg cttgcgccgg tgaaggccgg gccggattat atcgaccccg cctatcacag ggcggcgagt ggcgtggatt gtttcaatct cgacccctgg gccatgcgac cggaactcat cagtgcgctt tcctcccgca tgacggaaag cggcgcgcgt gttcttgtcg ccgaaggcat gatggggctt ttcgacgggg cgattgacgg taagggctct tctgccgatc tggcgcggct tctggatttg ccagtcgtgc tggtggtgga ttgcgcccgg caatcccact ccatcgcggc gctggtgtgg ggtttcagcc agtttcgcaa ggatgtgctg attgagggtg tcattctcaa tcgtgtcgga agtccccgcc atgaagccat gctgcggggc gctctggcac cccttggcgt cccggttctg ggtgcgctgc cgcgagatcc ggcactttcc ttgccggaaa ggcatctggg gctggtgcag gcggacgaac atgccggtct tgaaagcttt ctcgaacaag cggccgatgt gatggaagcg catatcgaca tggatgcgct tcagaccatc tggctacgcc cgaagcgcta tgacgccatg gccaatgtcg cgcgtctgaa accgcttgga aaccgcattg ccgtggcgcg ggacgatgcc tttgcttttg cctatatgca tctttttgan ggctggcgcn nnggggtgcg gaaatctctt tcttttcccc gctggcagat gaagcaccga anggcagatg cggangcaat ctatcttccc ngtggtnatc cagaacttca tgcgcaacga ttngnnngng catcccnttn ccgcacggnn atnggggang ctncngcnng nngngncncn gnctatggnn nnnnnngnnn nnnnnngnnn nnngnaaann nntgnnnnnn cgnngnnnnn nnnccnnnng gggntttnnn nnnnnnnnnn ntnnnnngnn natnnnnnnn nnnnncnnnn t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGFMIAAP ASGSGKTTVT LGLLRALKRR GEVLAPVKAG PDYIDPAYHR AASGVDCFNL DPWAMRPELI SALSSRMTES GARVLVAEGM MGLFDGAIDG KGSSADLARL LDLPVVLVVD CARQSHSIAA LVWGFSQFRK DVLIEGVILN RVGSPRHEAM LRGALAPLGV PVLGALPRDP ALSLPERHLG LVQADEHAGL ESFLEQAADV MEAHIDMDAL QTIWLRPKRY DAMANVARLK PLGNRIAVAR DDAFAFAYMH LFEGWRRRGA EISFFSPLAD EAPKADADAI YLPGGYPELH AQRLAGASRF RTAIGDAAAR GVTVYGECGG YMVLGKTLED AAGVHHPMLG LLPLETSFAR RKLHLGYRLL EPLGGLPWDM PLKAHEFHYA SIVREEKADR LFRVRDASGE NLGEAGLRVG SVSGSFMHVI DFSGEAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagggattc atgattgccg caccggcctc cgggtcgggc aagacgaccg tgaccttggg tttgttgcgc gccctgaaac gcaggggcga ggtgcttgcg ccggtgaagg ccgggccgga ttatatcgac cccgcctatc acagggcggc gagtggcgtg gattgtttca atctcgaccc ctgggccatg cgaccggaac tcatcagtgc gctttcctcc cgcatgacgg aaagcggcgc gcgtgttctt gtcgccgaag gcatgatggg gcttttcgac ggggcgattg acggtaaggg ctcttctgcc gatctggcgc ggcttctgga tttgccagtc gtgctggtgg tggattgcgc ccggcaatcc cactccatcg cggcgctggt gtggggtttc agccagtttc gcaaggatgt gctgattgag ggtgtcattc tcaatcgtgt cggaagtccc cgccatgaag ccatgctgcg gggcgctctg gcaccccttg gcgtcccggt tctgggtgcg ctgccgcgag atccggcact ttccttgccg gaaaggcatc tggggctggt gcaggcggac gaacatgccg gtcttgaaag ctttctcgaa caagcggccg atgtgatgga agcgcatatc gacatggatg cgcttcagac catctggcta cgcccgaagc gctatgacgc catggccaat gtcgcgcgtc tgaaaccgct tggaaaccgc attgccgtgg cgcgggacga tgcctttgct tttgcctata tgcatctttt tgaaggctgg cgcaggcggg gtgcggaaat ctctttcttt tccccgctgg cagatgaagc accgaaggca gatgcggatg caatctatct tcccggtggt tatccagaac ttcatgcgca acgattggcc ggtgcatccc gtttccgcac ggcgataggg gacgctgccg cgcgtggcgt cacggtctat ggcgagtgcg gcggctatat ggtcctcggg aaaacactgg aggatgcggc aggcgttcac catcccatgc tggggctttt gccattggaa acgagctttg ccaggcggaa actgcatctt ggctatcgcc tgctggagcc gcttggcgga ttgccgtggg acatgccttt gaaagcgcat gaattccatt atgcaagcat cgtgcgggaa gaaaaggccg accgcctgtt ccgggtgcgc gatgcatcgg gcgagaacct tggcgaagcg ggcttgcggg ttggttccgt ctccggctcc ttcatgcatg tcattgattt tagcggagaa gcggcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa