BrmeA.18184.a

Formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase) (EC 3.2.2.23) (DNA-(apurinic or apyrimidinic site) lyase mutM) (AP lyase mutM) (EC 4.2.99.18)

CENTER ID: BrmeA.18184.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18184.a.A1.GE33723 full length 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.2150
RefSeq: NP_540863.1
UniProt: Q8YED2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPELPEVETV RRGLQPFMEG ATVVRVEQNR PDLRFAFPEN FAERLSGRRI EALGRRAKYL TVHLDDGLSI ISHLGMSGSF RIEAEDAQGL PGGFHHERSK NSLHDHVVFH LMRPDGASAR IIYNDPRRFG FMLFAEKGAL EEHPLLKDLG VEPTGNLLSG EVLAALFKGR RTPLKAALLD QRLIAGLGNI YVCEALWRPG LSPMRAAGSV AGEMDVMERL AGAIRSVIAQ AIAAGGSSLK DYIQADGALG YFQHSFSVYG REGKPCRNPA CGGTVERVVQ SGRSTFFCAS CQT
NT Sequence
atgccggaac tgccagaggt cgaaacggtt cgccgtggtc tgcaaccctt catggaaggg gcaaccgttg tgcgggtgga gcagaatcgg cccgacctgc gctttgcctt tcctgaaaat tttgccgaac gcctttccgg caggcgcatc gaggcgcttg gccgccgtgc gaaatatctg accgtccatc tcgatgacgg gctttcgatc atcagccatc tcggcatgtc cggctcgttt cgcattgagg cggaggatgc gcaaggtttg ccgggcgggt ttcatcacga gcgctccaag aacagcctgc atgaccatgt ggtctttcac ctcatgcgcc cggacggtgc gtctgcacgc attatttata atgatccgcg ccgcttcggt ttcatgctct ttgccgaaaa gggcgcgctg gaggaacatc cgctcctgaa agaccttggt gtggagccga ccggcaatct cctgtcgggc gaggttctgg ccgcgctttt caagggacgc cgcacgcccc tgaaggccgc cttgctcgac cagaggctta tcgcggggct tggcaatatc tatgtctgcg aggcgctctg gcgcccaggg ctttcgccga tgcgcgcggc aggctcggtt gcaggcgaaa tggacgtgat ggagcggctt gccggggcca ttcgctccgt cattgcgcag gcgattgcag ccggaggctc cagccttaag gattacattc aggccgacgg tgcgctcggc tatttccagc actctttttc cgtctatggc cgcgagggta agccatgcag aaaccccgcc tgcggcggca ctgtcgagcg cgtggtgcaa tctggtcgtt cgactttttt ctgtgcctct tgccagacat ga
Details for BrmeA.18184.a.A1.GE33723
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPELPEVET VRRGLQPFME GATVVRVEQN RPDLRFAFPE NFAERLSGRR IEALGRRAKY LTVHLDDGLS IISHLGMSGS FRIEAEDAQG LPGGFHHERS KNSLHDHVVF HLMRPDGASA RIIYNDPRRF GFMLFAEKGA LEEHPLLKDL GVEPTGNLLS GEVLAALFKG RRTPLKAALL DQRLIAGLGN IYVCEALWRP GLSPMRAAGS VAGEMDVMER LAGAIRSVIA XAIAAGXSSL XDYIXADXAL GYFQHSFSVY GRXGKPCXNP ACXGTVERVV QSGRSTFFCA SCXT
Validated NT Sequence
attttgtnnn nnttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgg aactgccaga ggtcgaaacg gttcgccgtg gtctgcaacc cttcatggaa ggggcaaccg ttgtgcgggt ggagcagaat cggcccgacc tgcgctttgc ctttcctgaa aattttgccg aacgcctttc cggcaggcgc atcgaggcgc ttggccgccg tgcgaaatat ctgaccgtcc atctcgatga cgggctttcg atcatcagcc atctcggcat gtccggctcg tttcgcattg aggcggagga tgcgcaaggt ttgccgggcg ggtttcatca cgagcgctcc aagaacagcc tgcatgacca tgtggtcttt cacctcatgc gcccggacgg tgcgtctgca cgcattattt ataatgatcc gcgccgcttc ggtttcatgc tctttgccga aaagggcgcg ctggaggaac atccgctcct gaaagacctt ggtgtggagc cgaccggcaa tctcctgtcg ggcgaggttc tggccgcgct tttcaaggga cgccgcacgc ccctgaaggc cgccttgctc gaccagaggc ttatcgcggg gcttggcaat atctatgtct gcgaggcgct ctggcgccca gggctttcgc cgatgcgcgc ggcaggctcg gttgcaggcg aaatggacgt gatggagcgg cttgccgggg ccattcgctc cgtcattgcg cangcgattg cagccggang ctccagcctt aangattaca ttcangccga cngtgcgctc ggctatttcc agcactcttt ttccgtctat ggccgcnagg gtaagccatg canaaacccc gcctgcngcg gcactgtcga gcgcgtggtg caatctggtc gttcgacttt tttctgtgcc tcttgccnna cataatnnna gcacgaacaa gnnctgcagc caagcttctc gaggancnnc tgctnncnaa nncnnaanng nnnctgantn gnngctgcnn ngnnnnnnnn nnnnaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPELPEVET VRRGLQPFME GATVVRVEQN RPDLRFAFPE NFAERLSGRR IEALGRRAKY LTVHLDDGLS IISHLGMSGS FRIEAEDAQG LPGGFHHERS KNSLHDHVVF HLMRPDGASA RIIYNDPRRF GFMLFAEKGA LEEHPLLKDL GVEPTGNLLS GEVLAALFKG RRTPLKAALL DQRLIAGLGN IYVCEALWRP GLSPMRAAGS VAGEMDVMER LAGAIRSVIA QAIAAGGSSL KDYIQADGAL GYFQHSFSVY GREGKPCRNP ACGGTVERVV QSGRSTFFCA SCQT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggaactg ccagaggtcg aaacggttcg ccgtggtctg caacccttca tggaaggggc aaccgttgtg cgggtggagc agaatcggcc cgacctgcgc tttgcctttc ctgaaaattt tgccgaacgc ctttccggca ggcgcatcga ggcgcttggc cgccgtgcga aatatctgac cgtccatctc gatgacgggc tttcgatcat cagccatctc ggcatgtccg gctcgtttcg cattgaggcg gaggatgcgc aaggtttgcc gggcgggttt catcacgagc gctccaagaa cagcctgcat gaccatgtgg tctttcacct catgcgcccg gacggtgcgt ctgcacgcat tatttataat gatccgcgcc gcttcggttt catgctcttt gccgaaaagg gcgcgctgga ggaacatccg ctcctgaaag accttggtgt ggagccgacc ggcaatctcc tgtcgggcga ggttctggcc gcgcttttca agggacgccg cacgcccctg aaggccgcct tgctcgacca gaggcttatc gcggggcttg gcaatatcta tgtctgcgag gcgctctggc gcccagggct ttcgccgatg cgcgcggcag gctcggttgc aggcgaaatg gacgtgatgg agcggcttgc cggggccatt cgctccgtca ttgcgcaggc gattgcagcc ggaggctcca gccttaagga ttacattcag gccgacggtg cgctcggcta tttccagcac tctttttccg tctatggccg cgagggtaag ccatgcagaa accccgcctg cggcggcact gtcgagcgcg tggtgcaatc tggtcgttcg acttttttct gtgcctcttg ccagacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa