BrmeA.18183.a

[Protein-PII] uridylyltransferase (PII uridylyl-transferase) (EC 2.7.7.59) (UTase) (Uridylyl-removing enzyme)

CENTER ID:	BrmeA.18183.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18183.a.A2.GE33782	domain1		1	391

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18183.a.A2.PS01275	domain1		1	391

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_138771
PATRIC ID:	fig\|224914.11.peg.1995
RefSeq:	NP_540721.1
UniProt:	Q8YES3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAHDLKLEE IVNAETLRRK LNELADTADE SYTSLPMRKV VLQTLKDALA SGRANAEDML MKDGGGTLCA KRLCYLMDTL IDILFEFATT RAYPTRNPSK AENMALVAVG GYGRGGLAQG SDIDLLFLLP YKQTPWGEQV VEYTLYMLWD MGLKVGHSTR NIDECIRLAR EDMTIRTALL DARFLTGDKD LFRTLEIRFE EEIVKGTEPE FIQAKLAERD ARHRKAGETR YLVEPNVKEG KGGQRDLHTL FWITKYFYRV KTKEELVKLG VLSRAELKLF NKAEDFLWAV RCHMHFATLK AEERLSFDIQ PEIAQRLGYT AHPGQNYVER FMKHYFLVAK DVGDLTRIIC AALEEQQAKH VPGFNRIFLT FSRRKRKLSD DGAFISENHR INIARPDIFR QDPVNMIRLF HLADRHGLEF HPEAMQSLTR SLKLINADLR ENPEANRLFL EILTSPRNPE LILRRMNESG VLGKFIPDFG KIVAMMQFNM YHHYTVDEHL LRCIAVLSEI EHGELKTEHP LSNHLITTIK RDRNLLYVTL LLHDIAKGRP EDHSIAGARI ARRLCPRFGL TPSETETVEW LVREHLTMSM VAQSRDLNDR KTIIDFADTV QTMERLKLLL ILTVCDIKAV GPGIWNGWKG QLLRTLFYET ELVLTGGFSE LSRAARDKQA REALAERLSD WPKEERDAYL ALPYTNYFLT VSLDDQVRHA HFIRDADQQG RALVTMAKPH AFEAVTEITV LAPDHPRLLS VITGACAAAG GNIVDAQIFT TSDGRALDTI LISREFDTDD DERRRAERVG KVIEDVLSGK AHLPDMLAKR TKPKKAARAF KVEPRVEINN TLSNKFTVIE VEGLDRPGLL SELTGLISDL SLDIASAHIT TFGEKVIDSF YVTDLVGHKI SNATRQGNIK RKLLALLGAE NGARTNGRSP QAAA

NT Sequence

atgagcgcac acgacctgaa gctggaagag atcgtcaatg ccgaaacgct ccgtcgcaag ctcaatgaac tggcggacac ggccgatgaa agctacacca gccttcccat gcgcaaggtt gtgctccaga cgctgaagga tgcgctggcg agcgggcgcg ccaatgccga agacatgctg atgaaggatg gcggcggcac gctttgcgcc aagcggctct gctacctgat ggatacgctg atcgacatcc tgtttgaatt cgcaaccacc agggcctatc cgacgcgcaa tccctccaag gcggagaata tggcgctcgt ggcagtcggc ggctatgggc gcggcgggct tgcacagggt tccgacatcg accttctttt cctgcttccc tataaacaga cgccctgggg cgaacaggtt gtggaatata cgctctacat gctctgggac atggggctga aagtcggcca ttccacccgc aacatcgatg agtgcattcg tctggcgcgc gaggacatga ccattcgcac agcccttctc gatgcgcgct ttcttaccgg cgacaaggat ctgttccgca cacttgaaat ccgtttcgag gaggaaatcg tcaagggaac ggagccggaa ttcattcagg caaagcttgc cgagcgcgat gcacgccacc gcaaggcagg tgaaacacgc tatctggtcg aaccgaacgt caaggaaggc aagggcggac agcgcgacct gcacaccctg ttctggatca ccaaatattt ctaccgcgtg aagaccaagg aagagctggt caagcttggg gttctgtcgc gagcggaact gaaattgttc aacaaggctg aggattttct ctgggcggtg cggtgccata tgcattttgc gacgctgaaa gccgaagaac gcctttcctt cgacattcag ccggaaatcg cacagcgcct cggctatacg gcgcatcccg gccagaacta tgtcgaacgc ttcatgaagc attatttcct cgtcgcgaag gatgtgggtg acctcacccg catcatctgc gcggcgctgg aagaacagca ggcaaagcat gttccaggct tcaaccgcat cttcctgacc ttctcgcgcc gcaagcgcaa gctttcggat gatggcgctt tcatttcgga aaaccatcgc atcaatatcg cacggcccga tattttccgg caggacccgg tcaatatgat ccggctcttt caccttgccg accgccatgg gctggagttt catcccgaag caatgcagag cctcacccgc tcgctgaagc tcatcaatgc cgacctgcgc gagaacccgg aggccaaccg gcttttcctc gaaatcctca cctcgccgcg caatccggaa cttatcctgc gccgcatgaa cgaatccggc gtgcttggaa aattcattcc cgatttcggc aagatcgtcg cgatgatgca gttcaatatg tatcaccact atacggtgga cgagcatctg ttgcgctgca ttgccgtgct ttcggaaatc gagcacggcg agcttaaaac cgagcacccg ctttcaaacc atctcatcac cacgatcaag cgcgaccgca acctgcttta cgtgacgctt cttctgcacg atatcgccaa gggccgcccg gaagaccact ccatcgcggg cgcacgcatt gcccgcaggc tttgcccgcg cttcggcttg acaccatccg aaaccgagac cgtggaatgg ctggtgcgcg aacacctcac catgagcatg gtggcgcaat cgcgcgatct caacgaccgc aagaccatta tcgattttgc cgatacggtg cagacgatgg agcggctgaa gcttctcctg atcctcaccg tttgtgatat caaggcggtc ggccccggca tatggaacgg ctggaaaggc cagcttctgc gcacgctttt ctatgagacc gaactggtgc tgacgggcgg attttccgaa ttgtcgcggg ccgcccgcga caagcaggcg cgtgaggcgc tggccgagcg gctttccgac tggccgaagg aagagcgcga cgcctatctt gccctgccct ataccaatta tttcctcacc gtcagcctgg acgatcaggt gcgccatgcg catttcatac gcgatgccga ccagcagggc cgtgcgctcg tcaccatggc caagccgcac gcattcgagg ccgtgaccga aattaccgtt ctcgcgcccg accatccgcg tctcctttcg gtcattaccg gagcctgcgc ggcagcgggc ggcaatatcg tggatgcgca gatttttacc accagcgacg ggcgtgcgct ggacacgatc ctcatcagcc gcgaattcga caccgacgac gacgaacgcc gccgggccga gcgcgtgggc aaggtgatcg aggacgtgct ttccggcaag gcgcatctgc ccgatatgct ggcgaagcgc accaagccga agaaagctgc cagggctttc aaggtggagc cgcgtgtcga gatcaacaac acgctttcca acaaattcac ggttatcgaa gtggaaggtc tggaccggcc gggcctgctt tcagagctga ccggccttat ttccgacctt tcactggata tcgcttcggc ccatatcacc actttcggcg agaaggtgat cgacagtttt tatgtgacgg atctcgtggg ccacaagatt tcaaacgcca cccgccaggg caatatcaag cgcaagctcc ttgcccttct gggcgcagag aacggcgcca ggacaaacgg ccgctcgcca caggccgcag catag

Details for BrmeA.18183.a.A2.GE33782

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

tttccgaaat gaaagcgcca tcatccgaaa gcttgcgctt gcggcgcgag aaggtcagga agatgcggtt gaagcctgga acatgctttg cctgctgttc ttccagcgcc gcgcagatga tgcgggtgag gtcacccaca tccttcgcga cgaggaaata atgcttcatg aagcgttcga catagttctg gccgggatgc gccgtatagc cgaggcgctg tgcgatttcc ggctgaatgt cgaaggaaag gcgttcttcg gctttcagcg tcgcaaaatg catatggcac cgcaccgccc agagaaaatc ctcagccttg ttgaacaatt tcagttccgc tcgcgacaga accccaagct tgaccagctc ttccttggtc ttcacgcggt agaaatattt ggtgatccag aacagggtgt gcaggtcgcg ctgtccgccc ttgccttcct tgacgttcgg ttcgaccaga tagcgtgttt cacctgcctt gcggtggcgt gcatcgcgct cggcaagctt tgcctgaatg aattccggct ccgttccctt gacgatttcc tcctcgaaac ggatttcaag tgtgcggaac agatccttgt cgccggtaag aaagcgcgca tcgagaaggg ctgtgcgaat ggtcatgtcc tcgcgcgcca gacgaatgca ctcatcgatg ttgcgggtgg aatggccgac tttcagcccc atgtcccaga gcatgtagag cgtatattcc acaacctgtt cgccccaggg cgtctgttta tagggaagca ggaaaagaag gtcgatgtcg gaaccctgtg caagcccgcc gcgcccatag ccgccgactg ccacgagcgc catattctcc gccttggagg gattgcgcgt cggataggcc ctggtggttg cgaattcaaa caggatgtcg atcagcgtat ccatcaggta gcagagccgc ttggcgcaaa gcgtgccgcc gccatccttc atcagcatgt cttcggcatt ggcgcgcccg ctcgccagcg catccttcag cgtctggagc acaaccttgc gcatgggaag gctggtgtag ctttcatcgg ccgtgtccgc cagttcattg agcttgcgac ggagcgtttc ggcattgacg atctcttcca gcttcaggtc gtgtgcgctc atcgaaccag gaccctgggt ctgagcttcc agg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSAHDLKLE EIVNAETLRR KLNELADTAD ESYTSLPMRK VVLQTLKDAL ASGRANAEDM LMKDGGGTLC AKRLCYLMDT LIDILFEFAT TRAYPTRNPS KAENMALVAV GGYGRGGLAQ GSDIDLLFLL PYKQTPWGEQ VVEYTLYMLW DMGLKVGHST RNIDECIRLA REDMTIRTAL LDARFLTGDK DLFRTLEIRF EEEIVKGTEP EFIQAKLAER DARHRKAGET RYLVEPNVKE GKGGQRDLHT LFWITKYFYR VKTKEELVKL GVLSRAELKL FNKAEDFLWA VRCHMHFATL KAEERLSFDI QPEIAQRLGY TAHPGQNYVE RFMKHYFLVA KDVGDLTRII CAALEEQQAK HVPGFNRIFL TFSRRKRKLS DDGAFISENH RI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcacac gacctgaagc tggaagagat cgtcaatgcc gaaacgctcc gtcgcaagct caatgaactg gcggacacgg ccgatgaaag ctacaccagc cttcccatgc gcaaggttgt gctccagacg ctgaaggatg cgctggcgag cgggcgcgcc aatgccgaag acatgctgat gaaggatggc ggcggcacgc tttgcgccaa gcggctctgc tacctgatgg atacgctgat cgacatcctg tttgaattcg caaccaccag ggcctatccg acgcgcaatc cctccaaggc ggagaatatg gcgctcgtgg cagtcggcgg ctatgggcgc ggcgggcttg cacagggttc cgacatcgac cttcttttcc tgcttcccta taaacagacg ccctggggcg aacaggttgt ggaatatacg ctctacatgc tctgggacat ggggctgaaa gtcggccatt ccacccgcaa catcgatgag tgcattcgtc tggcgcgcga ggacatgacc attcgcacag cccttctcga tgcgcgcttt cttaccggcg acaaggatct gttccgcaca cttgaaatcc gtttcgagga ggaaatcgtc aagggaacgg agccggaatt cattcaggca aagcttgccg agcgcgatgc acgccaccgc aaggcaggtg aaacacgcta tctggtcgaa ccgaacgtca aggaaggcaa gggcggacag cgcgacctgc acaccctgtt ctggatcacc aaatatttct accgcgtgaa gaccaaggaa gagctggtca agcttggggt tctgtcgcga gcggaactga aattgttcaa caaggctgag gattttctct gggcggtgcg gtgccatatg cattttgcga cgctgaaagc cgaagaacgc ctttccttcg acattcagcc ggaaatcgca cagcgcctcg gctatacggc gcatcccggc cagaactatg tcgaacgctt catgaagcat tatttcctcg tcgcgaagga tgtgggtgac ctcacccgca tcatctgcgc ggcgctggaa gaacagcagg caaagcatgt tccaggcttc aaccgcatct tcctgacctt ctcgcgccgc aagcgcaagc tttcggatga tggcgctttc atttcggaaa accatcgcat ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BrmeA.18183.a.A2.PS01275

PURIFICATION DATe:	11/10/2011
CONCENTRATION:	26.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCACAC GACCTGAAGC TGGAAGAGAT CGTCAATGCC GAAACGCTCC GTCGCAAGCT CAATGAACTG GCGGACACGG CCGATGAAAG CTACACCAGC CTTCCCATGC GCAAGGTTGT GCTCCAGACG CTGAAGGATG CGCTGGCGAG CGGGCGCGCC AATGCCGAAG ACATGCTGAT GAAGGATGGC GGCGGCACGC TTTGCGCCAA GCGGCTCTGC TACCTGATGG ATACGCTGAT CGACATCCTG TTTGAATTCG CAACCACCAG GGCCTATCCG ACGCGCAATC CCTCCAAGGC GGAGAATATG GCGCTCGTGG CAGTCGGCGG CTATGGGCGC GGCGGGCTTG CACAGGGTTC CGACATCGAC CTTCTTTTCC TGCTTCCCTA TAAACAGACG CCCTGGGGCG AACAGGTTGT GGAATATACG CTCTACATGC TCTGGGACAT GGGGCTGAAA GTCGGCCATT CCACCCGCAA CATCGATGAG TGCATTCGTC TGGCGCGCGA GGACATGACC ATTCGCACAG CCCTTCTCGA TGCGCGCTTT CTTACCGGCG ACAAGGATCT GTTCCGCACA CTTGAAATCC GTTTCGAGGA GGAAATCGTC AAGGGAACGG AGCCGGAATT CATTCAGGCA AAGCTTGCCG AGCGCGATGC ACGCCACCGC AAGGCAGGTG AAACACGCTA TCTGGTCGAA CCGAACGTCA AGGAAGGCAA GGGCGGACAG CGCGACCTGC ACACCCTGTT CTGGATCACC AAATATTTCT ACCGCGTGAA GACCAAGGAA GAGCTGGTCA AGCTTGGGGT TCTGTCGCGA GCGGAACTGA AATTGTTCAA CAAGGCTGAG GATTTTCTCT GGGCGGTGCG GTGCCATATG CATTTTGCGA CGCTGAAAGC CGAAGAACGC CTTTCCTTCG ACATTCAGCC GGAAATCGCA CAGCGCCTCG GCTATACGGC GCATCCCGGC CAGAACTATG TCGAACGCTT CATGAAGCAT TATTTCCTCG TCGCGAAGGA TGTGGGTGAC CTCACCCGCA TCATCTGCGC GGCGCTGGAA GAACAGCAGG CAAAGCATGT TCCAGGCTTC AACCGCATCT TCCTGACCTT CTCGCGCCGC AAGCGCAAGC TTTCGGATGA TGGCGCTTTC ATTTCGGAAA ACCATCGCAT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA