BrmeA.18180.b

Phosphogluconate dehydratase (EC 4.2.1.12)

CENTER ID: BrmeA.18180.b
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18180.b.B1.GE36715 Full length( BrmeA.18180.b ) 1 607
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2823
BV-BRC: fig|224914.11.peg.2823
RefSeq: NP_541489.1
UniProt: Q8YCL7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTETAHKRI QEITERIRER SKPTREAYLD RVREAASNKP KRSFLGCANL AHGFAACGPH DKAALTADVV PNLGIITAYN DMLSAHQPFE TFPQLIKQAA REAGGVAQVA GGVPAMCDGV TQGFAGMELS LFSREVIAMA TAVGLSHDMF DAAVYLGVCD KIVPGLIVGA LTFGHLPPVF IPAGPMTSGL PNDEKAKTRQ LYAEGKVGRA ELLESESKSY HGPGTCTFYG TANSNQMLME IMGLHLPGAS FINPGTPLRD ALTREAAKRA LAITALGNEY TPVGEMIDER SIVNGVVGLN ATGGSTNHTI HLIAMAAAAG IKLTWHDISQ ISDVVPLLAR VYPNGLADVN HFHAAGGMGF LIRELLDGGL LHEDVRTVWG EGLRPYAIEP KLGPDGNVVR EAAPEKSGDD KVLAKIAAPF QPTGGIKVLS GNIGNAIIKV SAVKPKRHVI EAPAKVFNDQ AELQAAFKAG DLTGDFIAVV RYQGPKANGM PELHKLMTVL GILQDRGQRV ALVTDGRLSG ASGKVPSAIH VTPEALDGGM VGKIRTGDII RLDAINGTLE VLLNQAVLDS RIEERPDLSG NEYGMGRELF RAFRQIAGRA DEGASVF
NT Sequence
ATGTCCACCG AGACGGCACA TAAACGCATT CAGGAAATCA CCGAGCGTAT CCGTGAGCGC TCCAAACCTA CCCGCGAAGC CTATCTGGAC CGGGTGCGGG AGGCTGCCTC CAACAAGCCG AAGCGTTCCT TTCTGGGATG CGCGAATCTC GCTCATGGTT TTGCGGCCTG CGGCCCGCAT GACAAGGCCG CGCTGACCGC CGATGTCGTA CCGAACCTCG GCATCATCAC CGCCTATAAT GATATGCTTT CGGCGCACCA GCCTTTTGAA ACCTTCCCGC AACTCATTAA GCAGGCAGCG CGCGAAGCGG GCGGCGTGGC GCAGGTGGCC GGTGGTGTGC CTGCCATGTG CGATGGCGTC ACGCAAGGTT TTGCCGGCAT GGAACTCTCG CTCTTTTCGC GCGAAGTCAT TGCCATGGCC ACGGCCGTTG GCCTCTCGCA CGATATGTTC GATGCCGCCG TCTATCTCGG CGTCTGCGAC AAGATTGTGC CGGGACTAAT TGTCGGCGCG CTGACCTTCG GCCATCTGCC TCCCGTCTTC ATTCCAGCCG GCCCGATGAC CAGCGGACTG CCGAATGATG AAAAGGCGAA AACCCGCCAG CTTTATGCCG AAGGCAAGGT GGGCCGGGCG GAACTGCTGG AATCGGAATC GAAATCTTAT CACGGCCCTG GCACCTGCAC CTTCTACGGC ACCGCCAACT CCAACCAGAT GTTGATGGAG ATCATGGGCC TGCATCTTCC CGGCGCATCA TTCATCAATC CGGGAACGCC GCTGCGCGAT GCGCTGACAC GCGAAGCTGC CAAGCGCGCG CTTGCCATCA CCGCGCTCGG CAATGAATAT ACGCCGGTTG GCGAGATGAT TGATGAGCGA TCCATCGTCA ACGGCGTGGT CGGGCTGAAC GCCACTGGCG GCTCCACCAA CCACACAATC CACCTGATCG CCATGGCCGC CGCGGCTGGC ATCAAGCTGA CATGGCACGA TATTTCGCAG ATATCAGACG TGGTGCCGCT TCTGGCCCGC GTCTATCCGA ACGGACTTGC GGATGTGAAC CATTTCCACG CCGCAGGCGG CATGGGCTTC CTCATCCGCG AACTCCTCGA CGGCGGCCTG CTGCATGAAG ATGTGCGCAC CGTCTGGGGG GAAGGCCTGC GCCCCTATGC CATTGAGCCG AAGCTCGGCC CCGACGGCAA CGTCGTGCGC GAAGCCGCGC CGGAAAAAAG CGGCGACGAC AAGGTTCTGG CCAAGATTGC CGCACCATTC CAGCCAACCG GCGGCATCAA GGTGCTGAGC GGCAATATTG GCAATGCGAT CATCAAGGTT TCCGCCGTAA AGCCGAAACG TCATGTGATC GAAGCACCCG CGAAAGTCTT CAACGATCAG GCGGAATTGC AGGCCGCTTT CAAGGCGGGC GACCTCACCG GCGATTTTAT TGCCGTGGTG CGTTATCAGG GGCCAAAAGC CAATGGCATG CCCGAACTGC ACAAGCTGAT GACCGTTCTG GGCATTCTTC AGGATCGCGG CCAGCGCGTG GCGCTCGTCA CCGATGGGCG TCTGTCCGGT GCATCCGGCA AGGTTCCCTC TGCCATCCAC GTCACGCCGG AAGCACTTGA TGGCGGCATG GTCGGCAAAA TCCGCACCGG CGATATTATC CGCCTCGATG CAATCAACGG CACGCTGGAA GTCTTGCTCA ATCAGGCCGT GCTCGACAGC CGTATCGAGG AGCGGCCCGA TCTTTCCGGC AACGAATATG GCATGGGCCG CGAGCTGTTC CGTGCCTTCC GCCAGATTGC CGGGCGGGCG GATGAAGGCG CCTCGGTGTT C
Details for BrmeA.18180.b.B1.GE36715
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMS TETAHKRIQE ITERIRERSK PTREAYLDRV REAASNKPKR SFLGCANLAH GFAACGPHDK AALTADVVPN LGIITAYNDM LSAHQPFETF PQLIKQAARE AGGVAQVAGG VPAMCDGVTQ GFAGMELSLF SREVIAMATA VGLSHDMFDA AVYLGVCDKI VPGLIVGALT FGHLPPVFIP AGPMTSGLPN DEKAKTRQLY AEGKVGRAEL LESESKSYHG PGTCTFYGTA NSNQMLMEIM GLHLPGASFI NPGTPLRDAL TREAAKRALA ITALGNEYTP VGXMIDERSI VNGVXGLNAT GXSXXHTIXL IXXXXXXGIX LXWXDXXXXX XXX
Validated NT Sequence
cnctannnnn nttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgtccac cgagacggca cataaacgca ttcaggaaat caccgagcgt atccgtgagc gctccaaacc tacccgcgaa gcctatctgg accgggtgcg ggaggctgcc tccaacaagc cgaagcgttc ctttctggga tgcgcgaatc tcgctcatgg ttttgcggcc tgcggcccgc atgacaaggc cgcgctgacc gccgatgtcg taccgaacct cggcatcatc accgcctata atgatatgct ttcggcgcac cagccttttg aaaccttccc gcaactcatt aagcaggcag cgcgcgaagc gggcggcgtg gcgcaggtgg ccggtggtgt gcctgccatg tgcgatggcg tcacgcaagg ttttgccggc atggaactct cgctcttttc gcgcgaagtc attgccatgg ccacggccgt tggcctctcg cacgatatgt tcgatgccgc cgtctatctc ggcgtctgcg acaagattgt gccgggacta attgtcggcg cgctgacctt cggccatctg cctcccgtct tcattccagc cggcccgatg accagcggac tgccgaatga tgaaaaggcg aaaacccgcc agctttatgc cgaaggcaag gtgggccggg cggaactgct ggaatcggaa tcgaaatctt atcacggccc tggcacctgc accttctacg gcaccgccaa ctccaaccag atgttgatgg agatcatggg cctgcatctt cccggcgcat cattcatcaa tccgggaacg ccgctgcgcg atgcgctgac acgcgaagct gccaagcgcg cgcttgccat caccgcgctc ggcaatgaat atacgccggt tggcnagatg attgatgagc gatccatcgt caacggcgtg gncgggctga acgccactgg nngctccncc naccacacaa tccnnctgat cnccntggnn ncnnnngntg gcatcnngct gnnntggnnc gatntttnnn nnannnnnnn nnnnnnncc
Expected Protein Sequence
MAHHHHHHMS TETAHKRIQE ITERIRERSK PTREAYLDRV REAASNKPKR SFLGCANLAH GFAACGPHDK AALTADVVPN LGIITAYNDM LSAHQPFETF PQLIKQAARE AGGVAQVAGG VPAMCDGVTQ GFAGMELSLF SREVIAMATA VGLSHDMFDA AVYLGVCDKI VPGLIVGALT FGHLPPVFIP AGPMTSGLPN DEKAKTRQLY AEGKVGRAEL LESESKSYHG PGTCTFYGTA NSNQMLMEIM GLHLPGASFI NPGTPLRDAL TREAAKRALA ITALGNEYTP VGEMIDERSI VNGVVGLNAT GGSTNHTIHL IAMAAAAGIK LTWHDISQIS DVVPLLARVY PNGLADVNHF HAAGGMGFLI RELLDGGLLH EDVRTVWGEG LRPYAIEPKL GPDGNVVREA APEKSGDDKV LAKIAAPFQP TGGIKVLSGN IGNAIIKVSA VKPKRHVIEA PAKVFNDQAE LQAAFKAGDL TGDFIAVVRY QGPKANGMPE LHKLMTVLGI LQDRGQRVAL VTDGRLSGAS GKVPSAIHVT PEALDGGMVG KIRTGDIIRL DAINGTLEVL LNQAVLDSRI EERPDLSGNE YGMGRELFRA FRQIAGRADE GASVF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca ccgagacggc acataaacgc attcaggaaa tcaccgagcg tatccgtgag cgctccaaac ctacccgcga agcctatctg gaccgggtgc gggaggctgc ctccaacaag ccgaagcgtt cctttctggg atgcgcgaat ctcgctcatg gttttgcggc ctgcggcccg catgacaagg ccgcgctgac cgccgatgtc gtaccgaacc tcggcatcat caccgcctat aatgatatgc tttcggcgca ccagcctttt gaaaccttcc cgcaactcat taagcaggca gcgcgcgaag cgggcggcgt ggcgcaggtg gccggtggtg tgcctgccat gtgcgatggc gtcacgcaag gttttgccgg catggaactc tcgctctttt cgcgcgaagt cattgccatg gccacggccg ttggcctctc gcacgatatg ttcgatgccg ccgtctatct cggcgtctgc gacaagattg tgccgggact aattgtcggc gcgctgacct tcggccatct gcctcccgtc ttcattccag ccggcccgat gaccagcgga ctgccgaatg atgaaaaggc gaaaacccgc cagctttatg ccgaaggcaa ggtgggccgg gcggaactgc tggaatcgga atcgaaatct tatcacggcc ctggcacctg caccttctac ggcaccgcca actccaacca gatgttgatg gagatcatgg gcctgcatct tcccggcgca tcattcatca atccgggaac gccgctgcgc gatgcgctga cacgcgaagc tgccaagcgc gcgcttgcca tcaccgcgct cggcaatgaa tatacgccgg ttggcgagat gattgatgag cgatccatcg tcaacggcgt ggtcgggctg aacgccactg gcggctccac caaccacaca atccacctga tcgccatggc cgccgcggct ggcatcaagc tgacatggca cgatatttcg cagatatcag acgtggtgcc gcttctggcc cgcgtctatc cgaacggact tgcggatgtg aaccatttcc acgccgcagg cggcatgggc ttcctcatcc gcgaactcct cgacggcggc ctgctgcatg aagatgtgcg caccgtctgg ggggaaggcc tgcgccccta tgccattgag ccgaagctcg gccccgacgg caacgtcgtg cgcgaagccg cgccggaaaa aagcggcgac gacaaggttc tggccaagat tgccgcacca ttccagccaa ccggcggcat caaggtgctg agcggcaata ttggcaatgc gatcatcaag gtttccgccg taaagccgaa acgtcatgtg atcgaagcac ccgcgaaagt cttcaacgat caggcggaat tgcaggccgc tttcaaggcg ggcgacctca ccggcgattt tattgccgtg gtgcgttatc aggggccaaa agccaatggc atgcccgaac tgcacaagct gatgaccgtt ctgggcattc ttcaggatcg cggccagcgc gtggcgctcg tcaccgatgg gcgtctgtcc ggtgcatccg gcaaggttcc ctctgccatc cacgtcacgc cggaagcact tgatggcggc atggtcggca aaatccgcac cggcgatatt atccgcctcg atgcaatcaa cggcacgctg gaagtcttgc tcaatcaggc cgtgctcgac agccgtatcg aggagcggcc cgatctttcc ggcaacgaat atggcatggg ccgcgagctg ttccgtgcct tccgccagat tgccgggcgg gcggatgaag gcgcctcggt gttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat