BrmeA.18179.a

2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase)

CENTER ID:	BrmeA.18179.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18179.a.A1.GE33732	full length		1	555

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132150
PATRIC ID:	fig\|224914.11.peg.508
RefSeq:	NP_539368.2
UniProt:	Q8YIJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPIAGTKYAP FPAPQLDDRT WPSKRIEKAP IWCSVDLRDG NQALIDPMGH DRKERMFRLL IDMGFPEIEI GFPSASQTDF DFCRWAIEQG DVPDDVDLQV LVQCRPELIT RTFEALEGAK TPIIHFYNST SELQRRVVFA KDVGGIKQIA TDAAKMIMDM AAKAGGGYRF QYSPESFTGT ELDVALEICN AVIEIVKPTP DNKLIVNLPS TVEMNTPNVY ADQIEWMCRN LDNRESLIIS LHPHNDRGTG IAATELGLMA GADRGEGTLF GNGERTGNVD VVTLALNMYT QGIDPGLDCT DINRMKEVYE YSNQLKIAER HPYVGELVYT AFSGSHQDAI NKGMKARRSA NSPVWEVPYL PIDPQDVGRS YEAIIRINSQ SGKGGIAYIL QADYGLNLPR NLQVEFREII QHITDEEGKE LPSKRIYEEF QKLYVTQPDA RIKFVDHHTY PHPEQKGRRI LTAEITDNGV TKTIEGKGTG PIDGFVDALS KYLGVKMSVV DYSEHSLQQG SDASAISYVE MVYPGGKLFG VGINDNIVSA SLEAVVSAAN RVIAK

NT Sequence

ttgaaagccg ggaggaaacg agaaatgaac cagcctgtct ccacgccaaa gtccgtgtcc caagccgtat cggcacgcaa gggcatgcct attgccggta ccaaatacgc gccgttccct gcgccgcagc tcgatgaccg cacttggcct tccaaacgca tcgaaaaggc gccgatctgg tgttcggtcg atctgcgcga cggcaaccag gctttgatcg accccatggg gcatgaccgc aaggagcgca tgttccgcct gctgatcgat atgggtttcc cggaaatcga gatcggtttc ccgtccgctt cgcagacgga tttcgacttc tgccgctggg ccattgaaca gggcgatgtt cctgacgacg tggatttgca ggttctggtc cagtgccgtc ctgaattgat tacgcgcact ttcgaggcgc tggaaggcgc gaagacgccg atcatccatt tctacaattc caccagcgaa ttgcagcgcc gcgtggtgtt tgccaaggat gtcggcggca tcaagcagat tgcaaccgat gcggccaaga tgatcatgga tatggctgcc aaggctggcg gcggttatcg cttccagtat tcgccggaaa gctttaccgg caccgaactg gatgtggcgc tggaaatctg caatgcggtg atcgagatcg tgaagccgac gccggacaac aagctgatcg tcaacctgcc ttcgacggtg gagatgaaca cgccgaatgt ctatgccgac cagatcgaat ggatgtgccg caatctggac aatcgcgaga gcctgattat ctcgctgcat ccgcataatg accgcggcac gggcattgcc gccactgaac tgggcctgat ggcaggcgct gatcgcgggg aaggcacgct gttcggcaat ggcgagcgca cgggcaatgt ggatgtggtg acgctggcgc tcaacatgta cacgcagggc atcgaccccg ggctggattg caccgatatc aaccggatga aggaagtcta cgaatattcc aaccagttga agatcgccga gcgccatcct tatgtgggcg agctggtcta tacggctttc tccggctcgc atcaggatgc gatcaacaag ggcatgaaag cgcgccgttc ggccaattcg ccggtttggg aagttcctta cctgccgatc gatccgcagg atgtgggccg ctcctacgag gcgatcatcc gcatcaattc gcaatcgggc aagggcggta tcgcctatat cctgcaagcc gattacggcc tgaacctgcc gcgcaacctg caggtcgagt tccgcgaaat catccagcac attacggatg aagagggcaa ggagctgcca tcgaagcgca tttacgagga gttccagaaa ctctatgtga cgcagcccga tgcccgcatc aagttcgtgg atcaccacac ctatccccat cctgagcaaa agggccggcg catcctgacg gctgagatca ccgataacgg tgtcaccaag actatcgagg gcaagggcac gggaccgatc gatggtttcg tggatgcgct gtcgaaatat cttggcgtga aaatgtcggt ggtcgattat tccgaacatt cgctccagca gggttcggat gcttcggcca tttcctatgt cgagatggtt tatcccggcg gcaagctgtt cggtgtcggc atcaacgata atatcgtcag cgcctcgctt gaagccgtgg tgtcggcggc aaatcgtgtg attgcgaagt ag

Details for BrmeA.18179.a.A1.GE33732

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

ttntttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttgaaa gccgggagga aacgagaaat gaaccagcct gtctccacgc caaagtccgt gtcccaagcc gtatcggcac gcaagggcat gcctattgcc ggtaccaaat acgcgccgtt ccctgcgccg cagctcgatg accgcacttg gccttccaaa cgcatcgaaa aggcgccgat ctggtgttcg gtcgatctgc gcgacggcaa ccaggctttg atcgacccca tggggcatga ccgcaaggag cgcatgttcc gcctgctgat cgatatgggt ttcccggaaa tcgagatcgg tttcccgtcc gcttcgcaga cggatttcga cttctgccgc tgggccattg aacagggcga tgttcctgac gacgtggatt tgcaggttct ggtccagtgc cgtcctgaat tgattacgcg cactttcgag gcgctggaag gcgcgaagac gccgatcatc catttctaca attccaccag cgaattgcag cgccgcgtgg tgtttgccaa ggatgtcggc ggcatcaagc agattgcaac cgatgcggcc aagatgatca tggatatggc tgccaaggct ggcggcggtt atcgcttcca gtattcgccg gaaagcttta ccggcaccga actggatgtg gcgctggnna tctgcaatgc ggtgatcgag atcgtgaagc cgacgccgga caacaagctg atcgtcaacc tgccttcgac ggtggagatg aacacgccga atgtctatgc cgaccagatc gaatggatgt gccgcaatct ggacaatcgc gagagcctga ttatctcgct gcatccgcat natgaccgcg gcacgggcat tgccgccnct gaactgggcc tgatggcagg ngctgatcgc gggggcacgc tnttcggcaa tggcgagcgc acgggnnntg tggatgtggt gacgctggcg ctcaacatgn ncacgcnngg nntcnacccn nnncnngnnn gnnntnnnnn nntnnnnnnn nncnannnnt nnnnnnttna natcnncngn cntncnnntn nnngnnnnnn ntnnnnnnnn nnngnntttn nnnnnnnnnn nnnnntnann nnnnnnnnaa naa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKAGRKRE MNQPVSTPKS VSQAVSARKG MPIAGTKYAP FPAPQLDDRT WPSKRIEKAP IWCSVDLRDG NQALIDPMGH DRKERMFRLL IDMGFPEIEI GFPSASQTDF DFCRWAIEQG DVPDDVDLQV LVQCRPELIT RTFEALEGAK TPIIHFYNST SELQRRVVFA KDVGGIKQIA TDAAKMIMDM AAKAGGGYRF QYSPESFTGT ELDVALEICN AVIEIVKPTP DNKLIVNLPS TVEMNTPNVY ADQIEWMCRN LDNRESLIIS LHPHNDRGTG IAATELGLMA GADRGEGTLF GNGERTGNVD VVTLALNMYT QGIDPGLDCT DINRMKEVYE YSNQLKIAER HPYVGELVYT AFSGSHQDAI NKGMKARRSA NSPVWEVPYL PIDPQDVGRS YEAIIRINSQ SGKGGIAYIL QADYGLNLPR NLQVEFREII QHITDEEGKE LPSKRIYEEF QKLYVTQPDA RIKFVDHHTY PHPEQKGRRI LTAEITDNGV TKTIEGKGTG PIDGFVDALS KYLGVKMSVV DYSEHSLQQG SDASAISYVE MVYPGGK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaagcc gggaggaaac gagaaatgaa ccagcctgtc tccacgccaa agtccgtgtc ccaagccgta tcggcacgca agggcatgcc tattgccggt accaaatacg cgccgttccc tgcgccgcag ctcgatgacc gcacttggcc ttccaaacgc atcgaaaagg cgccgatctg gtgttcggtc gatctgcgcg acggcaacca ggctttgatc gaccccatgg ggcatgaccg caaggagcgc atgttccgcc tgctgatcga tatgggtttc ccggaaatcg agatcggttt cccgtccgct tcgcagacgg atttcgactt ctgccgctgg gccattgaac agggcgatgt tcctgacgac gtggatttgc aggttctggt ccagtgccgt cctgaattga ttacgcgcac tttcgaggcg ctggaaggcg cgaagacgcc gatcatccat ttctacaatt ccaccagcga attgcagcgc cgcgtggtgt ttgccaagga tgtcggcggc atcaagcaga ttgcaaccga tgcggccaag atgatcatgg atatggctgc caaggctggc ggcggttatc gcttccagta ttcgccggaa agctttaccg gcaccgaact ggatgtggcg ctggaaatct gcaatgcggt gatcgagatc gtgaagccga cgccggacaa caagctgatc gtcaacctgc cttcgacggt ggagatgaac acgccgaatg tctatgccga ccagatcgaa tggatgtgcc gcaatctgga caatcgcgag agcctgatta tctcgctgca tccgcataat gaccgcggca cgggcattgc cgccactgaa ctgggcctga tggcaggcgc tgatcgcggg gaaggcacgc tgttcggcaa tggcgagcgc acgggcaatg tggatgtggt gacgctggcg ctcaacatgt acacgcaggg catcgacccc gggctggatt gcaccgatat caaccggatg aaggaagtct acgaatattc caaccagttg aagatcgccg agcgccatcc ttatgtgggc gagctggtct atacggcttt ctccggctcg catcaggatg cgatcaacaa gggcatgaaa gcgcgccgtt cggccaattc gccggtttgg gaagttcctt acctgccgat cgatccgcag gatgtgggcc gctcctacga ggcgatcatc cgcatcaatt cgcaatcggg caagggcggt atcgcctata tcctgcaagc cgattacggc ctgaacctgc cgcgcaacct gcaggtcgag ttccgcgaaa tcatccagca cattacggat gaagagggca aggagctgcc atcgaagcgc atttacgagg agttccagaa actctatgtg acgcagcccg atgcccgcat caagttcgtg gatcaccaca cctatcccca tcctgagcaa aagggccggc gcatcctgac ggctgagatc accgataacg gtgtcaccaa gactatcgag ggcaagggca cgggaccgat cgatggtttc gtggatgcgc tgtcgaaata tcttggcgtg aaaatgtcgg tggtcgatta ttccgaacat tcgctccagc agggttcgga tgcttcggcc atttcctatg tcgagatggt ttatcccggc ggcaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa