BrmeA.18178.a

Lon protease (EC 3.4.21.53) (ATP-dependent protease La)

CENTER ID: BrmeA.18178.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18178.a.A1.GE33731 full length 1 812
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.983
RefSeq: NP_539793.1
UniProt: Q8YHC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGIEQKTPV GGSETGGADG LYAVLPLRDI VVFPHMIVPL FVGREKSIRA LEEVMGVDKQ ILLATQKNAA DDDPAPDAIY EIGTIANVLQ LLKLPDGTVK VLVEGTARAK ISKFTDREDY HEAYAAALQE PEEDAVEIEA LARSVVSDFE NYVKLNKKIS PEVVGTASQI DDYSKLADTV ASHLAIKIPE KQEMLSVLSV RERLEKALSF MEAEISVLQV EKRIRSRVKR QMEKTQREYY LNEQMKAIQK ELGDSEDGRD EVAEIEERIT KTKLSKEARE KALAELKKLR SMSPMSAEAT VVRNYLDWLL SIPWGKKSKV KQDLNFAQEV LDAEHFGLGK VKERIVEYLA VQARSTKIKG PILCLVGPPG VGKTSLARSI AKATGREYVR MSLGGVRDEA EIRGHRRTYI GSMPGKVIQS MKKAKKSNPL FLLDEIDKMG QDFRGDPSSA MLEVLDPKQN ATFMDHYLEV EYDLSNVMFV TTANTMNIPG PLLDRMEIIR IAGYTEDEKL EIAKRHLLPK AIKDHALQPK EFSVTEDALR NVIRHYTREA GVRSLEREVM TLARKAVTEI LKTKKKSVKI TDKNLSDYLG VEKFRFGQID GEDQVGVVTG LAWTEVGGEL LTIEGVMMPG KGRMTVTGNL RDVMKESISA AASYVRSRAI DFGIEPPLFD KRDIHVHVPE GATPKDGPSA GIAMVTAIVS VLTGIPVRKD IAMTGEVTLR GRVLPIGGLK EKLLATLRGG IKKVLIPEEN AKDLAEIPDN VKNNLEIVPV SRVGEVLKHA LVRQPEPIEW TEQENPTAVP PVEDEAGASL AH
NT Sequence
ttgggtcgga aggcccgaga aaggactagt gttatgacgg gtattgaaca gaaaacaccg gttggtggtt ctgaaacggg tggtgcggac gggctctatg ctgtcttgcc gctgcgtgac atcgtcgtct ttccccatat gatcgtcccg ctttttgtgg gccgcgaaaa atccattcgc gcgcttgaag aagtgatggg cgttgacaag cagatattgc ttgccaccca gaagaacgct gccgatgacg atccggcgcc ggacgcgatc tatgagattg gtacgatcgc caatgtgttg cagcttctga aactgccgga cggcaccgtc aaggtgctgg tcgaaggcac ggcacgcgcc aaaatttcca agtttaccga tcgtgaagat tatcacgagg cttatgctgc ggctctgcag gagccggagg aagatgctgt cgagatcgag gcactggccc gctcggtggt ttccgacttt gaaaattacg tcaagctgaa caagaagatt tcgccggaag tggttggcac ggccagccag atcgacgatt attccaagct tgccgatacg gttgcctcgc accttgcgat caaaatccct gaaaagcagg aaatgctgtc ggttctttcg gtgcgcgagc gccttgagaa agccctttcc ttcatggaag ccgaaatttc tgttctacag gttgagaagc gtattcgcag ccgcgtcaag cgccagatgg agaagacgca gcgcgaatat tatctcaatg agcagatgaa ggcgatccag aaggagcttg gcgacagtga ggacggccgt gatgaagtgg ccgaaatcga agagcgcatc accaagacca agctcagcaa ggaagcgcgc gaaaaagctc tggccgagct gaagaaactg cgcagcatga gcccgatgtc cgctgaagcg acggtggttc gcaattatct cgactggttg ctctccattc catggggcaa gaagtcgaag gtgaagcagg atctgaactt tgcgcaggaa gtgctggatg cggagcattt cggccttggc aaggtcaagg aacgcatcgt cgaatatctg gcggtgcagg cccgttcgac caagatcaag ggtccgatcc tctgcctcgt tggccctccc ggcgtcggca agacctcgct tgcgcgctcg attgccaagg caacgggccg cgaatatgtc cgcatgtcgc ttggcggcgt acgcgacgag gctgaaatcc gcggtcatcg ccgcacctat atcggctcga tgcccggcaa ggtcatccag tcgatgaaga aggcgaagaa gtccaatccg cttttcctgc tcgatgaaat cgacaagatg ggccaggatt tccgcggcga tccgtcttcg gccatgctgg aggtgctgga cccgaaacag aacgcgacct tcatggatca ctaccttgaa gttgagtatg atctatcgaa cgtcatgttc gtgacgaccg ccaatacgat gaatattccc ggtccacttc tggatcgtat ggagatcatc cgtatcgccg gttacacgga agacgaaaag ctggagatcg ccaagcggca cctgttgccg aaggccatca aggaccatgc cctgcaaccg aaggagtttt cggttacgga agatgcgctg cgcaacgtta tccgccatta tacgcgggaa gcgggcgtgc gtagccttga acgcgaggtg atgacccttg cgcgcaaggc cgtgacggaa atcctgaaga cgaagaagaa gtcggtaaag attaccgaca agaacctctc cgattacctt ggtgtggaga agttccgctt cggtcagatc gacggtgaag atcaggtggg tgtcgtgact ggccttgcct ggaccgaagt cggcggtgag cttttgacca tcgaaggcgt catgatgccg ggtaagggcc gcatgacggt tacgggtaat ctccgtgacg tgatgaagga atcgatttcg gccgcggcat cctatgtccg ctcgcgcgcg atcgatttcg gcatcgagcc tccgctgttc gacaagcgcg atatccacgt gcacgtgccg gaaggcgcga cgccgaagga tggtccttct gccggtattg ccatggttac ggccatcgtc tccgtgctga cgggtattcc cgttcgcaag gacatcgcca tgacgggtga agtcacgttg cgcggtcggg ttctgccaat cggcgggttg aaggaaaagc tgcttgcgac cttgcgcggc ggtatcaaga aggttctgat cccggaagag aacgccaagg atctggcgga aatcccggac aatgtgaaga acaatcttga gatcgttccg gtatcccgcg tcggtgaagt gctgaagcac gcgctcgtgc gccagcctga accgattgaa tggaccgagc aggagaatcc cactgccgtg cctccggtgg aggatgaagc aggggcttcg ctggcccatt aa
Details for BrmeA.18178.a.A1.GE33731
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 92kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 34
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLGRKARER TSVMTGIEQK TPVGGSETGG ADGLYAVLPL RDIVVFPHMI VPLFVGREKS IRALEEVMGV DKQILLATQK NAADDDPAPD AIYEIGTIAN VLQLLKLPDG TVKVLVEGTA RAKISKFTDR EDYHEAYAAA LQEPEEDAVE IEALARSVVS DFENYVKLNK KISPEVVGTA SQIDDYSKLA DTVASHLAIK IPEKQEMLSV LSVRERLEKA LSFMEAEISV LQVEKRIRSR VKRQMEKTQR EYYLNEQMXG DPEXAWRQ
Validated NT Sequence
attttgnnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg gtcggaaggc ccgagaaagg actagtgtta tgacgggtat tgaacagaaa acaccggttg gtggttctga aacgggtggt gcggacgggc tctatgctgt cttgccgctg cgtgacatcg tcgtctttcc ccatatgatc gtcccgcttt ttgtgggccg cgaaaaatcc attcgcgcgc ttgaagaagt gatgggcgtt gacaagcaga tattgcttgc cacccagaag aacgctgccg atgacgatcc ggcgccggac gcgatctatg agattggtac gatcgccaat gtgttgcagc ttctgaaact gccggacggc accgtcaagg tgctggtcga aggcacggca cgcgccaaaa tttccaagtt taccgatcgt gaagattatc acgaggctta tgctgcggct ctgcaggagc cggaggaaga tgctgtcgag atcgaggcac tggcccgctc ggtggtttcc gactttgaaa attacgtcaa gctgaacaag aagatttcgc cggaagtggt tggcacggcc agccagatcg acgattattc caagcttgcc gatacggttg cctcgcacct tgcgatcaaa atccctgaaa agcaggaaat gctgtcggtt ctttcggtgc gcgagcgcct tgagaaagcc ctttccttca tggaagccga aatttctgtt ctacaggttg agaagcgtat tcgcagccgc gtcaagcgcc agatggagaa gacgcagcgc gaatattatc tcaatgagca gatgaanggn gatccagaan gagcttggcg acagtgagga cngccgtgat gaagtggccg aaatcgaaga gcgcatcacn nngancnagc tcagcaagga agcgcgcgaa aaanctctng nnnganctga anaaactgcg cagcatgagc ccgatgnccg ctgaagcgan ngtggtncgc nntnnnnncg actggtngcn nncnttncat ggnnnnagnc naannnancn nntctnnnct ttncncngnn ngcnnnngng nnttcngcnn nnnnnnnnnn nnatcnnnnn annnntnnnn nnnngcnnnn nnnnannnng nnnnnnannn nnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGRKARER TSVMTGIEQK TPVGGSETGG ADGLYAVLPL RDIVVFPHMI VPLFVGREKS IRALEEVMGV DKQILLATQK NAADDDPAPD AIYEIGTIAN VLQLLKLPDG TVKVLVEGTA RAKISKFTDR EDYHEAYAAA LQEPEEDAVE IEALARSVVS DFENYVKLNK KISPEVVGTA SQIDDYSKLA DTVASHLAIK IPEKQEMLSV LSVRERLEKA LSFMEAEISV LQVEKRIRSR VKRQMEKTQR EYYLNEQMKA IQKELGDSED GRDEVAEIEE RITKTKLSKE AREKALAELK KLRSMSPMSA EATVVRNYLD WLLSIPWGKK SKVKQDLNFA QEVLDAEHFG LGKVKERIVE YLAVQARSTK IKGPILCLVG PPGVGKTSLA RSIAKATGRE YVRMSLGGVR DEAEIRGHRR TYIGSMPGKV IQSMKKAKKS NPLFLLDEID KMGQDFRGDP SSAMLEVLDP KQNATFMDHY LEVEYDLSNV MFVTTANTMN IPGPLLDRME IIRIAGYTED EKLEIAKRHL LPKAIKDHAL QPKEFSVTED ALRNVIRHYT REAGVRSLER EVMTLARKAV TEILKTKKKS VKITDKNLSD YLGVEKFRFG QIDGEDQVGV VTGLAWTEVG GELLTIEGVM MPGKGRMTVT GNLRDVMKES ISAAASYVRS RAIDFGIEPP LFDKRDIHVH VPEGATPKDG PSAGIAMVTA IVSVLTGIPV RKDIAMTGEV TLRGRVLPIG GLKEKLLATL RGGIKKVLIP EENAKDLAEI PDNVKNNLEI VPVSRVGEVL KHALVRQPEP IEWTEQENPT AVPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgggtcgg aaggcccgag aaaggactag tgttatgacg ggtattgaac agaaaacacc ggttggtggt tctgaaacgg gtggtgcgga cgggctctat gctgtcttgc cgctgcgtga catcgtcgtc tttccccata tgatcgtccc gctttttgtg ggccgcgaaa aatccattcg cgcgcttgaa gaagtgatgg gcgttgacaa gcagatattg cttgccaccc agaagaacgc tgccgatgac gatccggcgc cggacgcgat ctatgagatt ggtacgatcg ccaatgtgtt gcagcttctg aaactgccgg acggcaccgt caaggtgctg gtcgaaggca cggcacgcgc caaaatttcc aagtttaccg atcgtgaaga ttatcacgag gcttatgctg cggctctgca ggagccggag gaagatgctg tcgagatcga ggcactggcc cgctcggtgg tttccgactt tgaaaattac gtcaagctga acaagaagat ttcgccggaa gtggttggca cggccagcca gatcgacgat tattccaagc ttgccgatac ggttgcctcg caccttgcga tcaaaatccc tgaaaagcag gaaatgctgt cggttctttc ggtgcgcgag cgccttgaga aagccctttc cttcatggaa gccgaaattt ctgttctaca ggttgagaag cgtattcgca gccgcgtcaa gcgccagatg gagaagacgc agcgcgaata ttatctcaat gagcagatga aggcgatcca gaaggagctt ggcgacagtg aggacggccg tgatgaagtg gccgaaatcg aagagcgcat caccaagacc aagctcagca aggaagcgcg cgaaaaagct ctggccgagc tgaagaaact gcgcagcatg agcccgatgt ccgctgaagc gacggtggtt cgcaattatc tcgactggtt gctctccatt ccatggggca agaagtcgaa ggtgaagcag gatctgaact ttgcgcagga agtgctggat gcggagcatt tcggccttgg caaggtcaag gaacgcatcg tcgaatatct ggcggtgcag gcccgttcga ccaagatcaa gggtccgatc ctctgcctcg ttggccctcc cggcgtcggc aagacctcgc ttgcgcgctc gattgccaag gcaacgggcc gcgaatatgt ccgcatgtcg cttggcggcg tacgcgacga ggctgaaatc cgcggtcatc gccgcaccta tatcggctcg atgcccggca aggtcatcca gtcgatgaag aaggcgaaga agtccaatcc gcttttcctg ctcgatgaaa tcgacaagat gggccaggat ttccgcggcg atccgtcttc ggccatgctg gaggtgctgg acccgaaaca gaacgcgacc ttcatggatc actaccttga agttgagtat gatctatcga acgtcatgtt cgtgacgacc gccaatacga tgaatattcc cggtccactt ctggatcgta tggagatcat ccgtatcgcc ggttacacgg aagacgaaaa gctggagatc gccaagcggc acctgttgcc gaaggccatc aaggaccatg ccctgcaacc gaaggagttt tcggttacgg aagatgcgct gcgcaacgtt atccgccatt atacgcggga agcgggcgtg cgtagccttg aacgcgaggt gatgaccctt gcgcgcaagg ccgtgacgga aatcctgaag acgaagaaga agtcggtaaa gattaccgac aagaacctct ccgattacct tggtgtggag aagttccgct tcggtcagat cgacggtgaa gatcaggtgg gtgtcgtgac tggccttgcc tggaccgaag tcggcggtga gcttttgacc atcgaaggcg tcatgatgcc gggtaagggc cgcatgacgg ttacgggtaa tctccgtgac gtgatgaagg aatcgatttc ggccgcggca tcctatgtcc gctcgcgcgc gatcgatttc ggcatcgagc ctccgctgtt cgacaagcgc gatatccacg tgcacgtgcc ggaaggcgcg acgccgaagg atggtccttc tgccggtatt gccatggtta cggccatcgt ctccgtgctg acgggtattc ccgttcgcaa ggacatcgcc atgacgggtg aagtcacgtt gcgcggtcgg gttctgccaa tcggcgggtt gaaggaaaag ctgcttgcga ccttgcgcgg cggtatcaag aaggttctga tcccggaaga gaacgccaag gatctggcgg aaatcccgga caatgtgaag aacaatcttg agatcgttcc ggtatcccgc gtcggtgaag tgctgaagca cgcgctcgtg cgccagcctg aaccgattga atggaccgag caggagaatc ccactgccgt gcctccgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa