BrmeA.18161.a

Ribulose-phosphate 3-epimerase (EC 5.1.3.1)

CENTER ID: BrmeA.18161.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18161.a.A1.GE33795 full length 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.1251
RefSeq: NP_540033.1
UniProt: Q8YGP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
METGFWIKTR ASKHLERFQR FRFNRNRSRI FAVPVIRASH AAKETIAMRP IAIAPSILSA DFARLGEEVD AVLEAGADWV HIDVMDGHFV PNITFGPQVV KAIRPRTKAF FDAHLMIAPC DPYLEPFAEA GCDLITVHAE AGPHTHRSLQ SIRALGKKAG LALNPATPAE ALTNVLDDLD LILVMTVNPG FGGQKFIAPM LDKIRRVREM VGDRPIDIEV DGGITPETAG SVVAAGANIL VAGSAVYKGN SVESYRANID AIRAAAEAAR NR
NT Sequence
gtggaaaccg gtttttggat aaaaacacgc gccagcaaac acttagagcg gttccaacga ttccgcttta accggaaccg ctctaggatt tttgctgttc ctgtgatacg ggcaagtcac gctgccaagg agactatcgc catgcgcccc atcgccattg ccccttccat tctttccgcc gattttgccc gtcttggcga agaagtcgat gccgtgctgg aagccggtgc cgattgggtg catatcgacg tcatggacgg gcatttcgtg cccaacatca cctttggccc gcaggtggtg aaggcaatcc gcccgcgtac caaggcattc ttcgatgcgc atttgatgat tgcgccctgc gatccctatc tggaaccttt tgccgaagcg ggatgtgatc tcatcaccgt tcatgcggaa gccgggccgc acacgcatcg ctcgctgcaa tcgatccgtg cgcttggcaa gaaagccggt cttgcgctga accctgccac gcccgccgag gcgctcacca atgtgctgga tgaccttgac ctcatcctcg tgatgacggt caatccgggt ttcggcggcc agaagttcat cgcgccgatg ctggacaaga tcaggcgcgt gcgcgaaatg gtcggcgacc gccctatcga cattgaggtc gacggcggca ttacgcccga aaccgcaggt tcggtcgttg ccgctggcgc caacattctc gtggccggtt cggcggtcta caaaggtaat tcggtcgaga gttaccgtgc caatatcgac gccattcgtg ctgcggccga agccgcgcgt aatcgttaa
Details for BrmeA.18161.a.A1.GE33795
HARVESTED ON: 10/4/2011
SEQUENCED ON: 10/7/2011
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 85
PERCENT COVERAGE: 107
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVETGFWIK TRASKHLERF QRFRFNPNRS RIFAVPVIRA SHAAKETIAM RPIAIAPSIL SADFARLGEE VDAVLEAGAD WVHIDVMDGH FVPNITFGPQ VVKAIRPRTK AFFDAHLMIA PCDPYLEPFA EAGCDLITVH AEAGPHTHRS LQSIRALGKK AGLALNPATP AEALTNVLDD LDLILVMTVN PGFGGQKFIA PMLDKIRRVR EMVGDRPIDI EVDXGITPET AXRSLPLAPT FSWXVRRSTK VIRSXVTVXI STPFXLXPXX RNXXNSXXVA XXXXXXAXXX XXXXXC
Validated NT Sequence
ttttntttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg aaaccggttt ttggataaaa acacgcgcca gcaaacactt agagcggttc caacgattcc gctttaaccc gaaccgctct aggatttttg ctgttcctgt gatacgggca agtcacgctg ccaaggagac tatcgccatg cgccccatcg ccattgcccc ttccattctt tccgccgatt ttgcccgtct tggcgaagaa gtcgatgccg tgctggaagc cggtgccgat tgggtgcata tcgacgtcat ggacgggcat ttcgtgccca acatcacctt tggcccgcag gtggtgaagg caatccgccc gcgtaccaag gcattcttcg atgcgcattt gatgattgcg ccctgcgatc cctatctgga accttttgcc gaagcgggat gtgatctcat caccgttcat gcggaagccg ggccgcacac gcatcgctcg ctgcaatcga tccgtgcgct tggcaagaaa gccggtcttg cgctgaaccc tgccacgccc gccgaggcgc tcaccaatgt gctggatgac cttgacctca tcctcgtgat gacggtcaat ccgggtttcg gcggccagaa gttcatcgcg ccgatgctgg acaagatcag gcgcgtgcgc gaaatggtcg gcgaccgccc tatcgacatt gaggtcgacn gcggcattac gcccgaaacc gcnnntcggt cgttgccgct ggcgccaaca ttctcgtggn cggttcggcg gtctacaaag gtaattcggt cganagttac cgtgncaata tcgacgccat tcntgctgcn gccgannncg cgtaatcnnn nnaacagcnc nanagttgca ncnnntntnn ngnnnnntgc tnnnnaagnc ngaangnnnt nnnnntgct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVETGFWIK TRASKHLERF QRFRFNRNRS RIFAVPVIRA SHAAKETIAM RPIAIAPSIL SADFARLGEE VDAVLEAGAD WVHIDVMDGH FVPNITFGPQ VVKAIRPRTK AFFDAHLMIA PCDPYLEPFA EAGCDLITVH AEAGPHTHRS LQSIRALGKK AGLALNPATP AEALTNVLDD LDLILVMTVN PGFGGQKFIA PMLDKIRRVR EMVGDRPIDI EVDGGITPET AGSVVAAGAN ILVAGSAVYK GNSVESYRAN IDAIRAAAEA ARNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaaacc ggtttttgga taaaaacacg cgccagcaaa cacttagagc ggttccaacg attccgcttt aaccggaacc gctctaggat ttttgctgtt cctgtgatac gggcaagtca cgctgccaag gagactatcg ccatgcgccc catcgccatt gccccttcca ttctttccgc cgattttgcc cgtcttggcg aagaagtcga tgccgtgctg gaagccggtg ccgattgggt gcatatcgac gtcatggacg ggcatttcgt gcccaacatc acctttggcc cgcaggtggt gaaggcaatc cgcccgcgta ccaaggcatt cttcgatgcg catttgatga ttgcgccctg cgatccctat ctggaacctt ttgccgaagc gggatgtgat ctcatcaccg ttcatgcgga agccgggccg cacacgcatc gctcgctgca atcgatccgt gcgcttggca agaaagccgg tcttgcgctg aaccctgcca cgcccgccga ggcgctcacc aatgtgctgg atgaccttga cctcatcctc gtgatgacgg tcaatccggg tttcggcggc cagaagttca tcgcgccgat gctggacaag atcaggcgcg tgcgcgaaat ggtcggcgac cgccctatcg acattgaggt cgacggcggc attacgcccg aaaccgcagg ttcggtcgtt gccgctggcg ccaacattct cgtggccggt tcggcggtct acaaaggtaa ttcggtcgag agttaccgtg ccaatatcga cgccattcgt gctgcggccg aagccgcgcg taatcgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa