BrmeA.18154.a
Iole protein
| CENTER ID: | BrmeA.18154.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18154.a.A1.GE33805 | full length | 1 | 300 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18154.a.A1.PS01279 | full length | 1 | 300 |
Structures
9ZMU
DEPOSITED: 12/11/2025
DETERMINATION: XRay
CLONE: BrmeA.18154.a.B2.GE45387
PROTEIN: BrmeA.18154.a.B2.PW39413
9ZMV
DEPOSITED: 12/11/2025
DETERMINATION: XRay
CLONE: BrmeA.18154.a.B2.GE45387
PROTEIN: BrmeA.18154.a.B2.PW39413
9ZMW
DEPOSITED: 12/11/2025
DETERMINATION: XRay
CLONE: BrmeA.18154.a.B2.GE45387
PROTEIN: BrmeA.18154.a.B2.PW39413
9ZMX
DEPOSITED: 12/11/2025
DETERMINATION: XRay
CLONE: BrmeA.18154.a.B2.GE45387
PROTEIN: BrmeA.18154.a.B2.PW39413
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|224914.11.peg.2883 |
| RefSeq: | NP_541548.1 |
| UniProt: | Q8YCG0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILYGTNPIA WSNDDDRSLG AHITLDQCLD EAAKIGFDGI EKGHKFPQEA AALKAVLEPR GLRYVSGWHS LNLLVNSVEE EKAAMQPALD LLKAMGSKVI IVCETSNAIH GDNDKPLQER PVLEDARWEA FGLGVEALAA HAAAQGITLV YHHHMGTIVE TEDEIDRLMR FTGPHTRLLL DTGHCLFGRG NPERVAQKHI ARVGHIHAKN IRRDIVQDVR DQNLSFLEGV RRGVFTVPGD TEGSVDFVPV LKIAAEHGYQ GWLVIEAEQD PDVRNPFEYQ SLGLKSLKAF AREAGLDKGV
NT Sequence
atgatccttt acggcaccaa ccccattgca tggtcaaacg atgacgaccg cagcctcggc gcgcatatca cgcttgatca atgcctcgac gaagccgcaa aaatcggctt cgacgggatc gaaaaaggtc ataaattccc gcaggaagct gccgctctta aagcagtgct ggaaccgcgc gggttgcgct atgtttccgg ctggcattcg ctcaatctgc tggtcaattc cgtcgaggaa gaaaaggcgg cgatgcagcc cgcgcttgat ctcctgaagg cgatgggctc gaaagttatc atcgtctgcg aaacctcgaa cgccattcat ggcgacaatg acaagccgct gcaagagcgc cctgttctgg aagatgcccg ctgggaagcc ttcggccttg gtgtggaagc actggccgca catgccgccg cgcaaggcat cacgctcgtc tatcatcacc acatgggtac aatcgtggaa acggaagacg agatcgaccg tctgatgcgt ttcaccgggc cgcacaccag gcttttgctc gatacgggcc attgcctgtt cggacgcggc aatccagaac gggtggcgca aaagcatata gcgcgcgtcg gccatatcca tgccaagaat attcgccgcg atatagtgca ggacgtgcgc gaccagaacc tgtccttcct tgaaggtgtg cggcgcggtg tgttcaccgt accgggtgat acggaaggct cggtagattt cgtgcccgta ctgaaaattg ccgcagagca cggctatcag ggatggctcg ttatcgaagc cgaacaagat ccagatgtgc gcaatccttt tgaatatcag tcgcttgggc tgaaatccct gaaggctttc gcgcgcgagg caggactcga caagggagtt tag
Details for BrmeA.18154.a.A1.GE33805
| HARVESTED ON: | 10/4/2011 |
| SEQUENCED ON: | 10/7/2011 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MILYGTNPIA WSNDDDRSLG AHITLDQCLD EAAKIGFDGI EKGHKFPQEA AALKAVLEPR GLRYVSGWHS LNLLVNSVEE EKAAMQPALD LLKAMGSKVI IVCETSNAIH GDNDKPLQER PVLEDARWEA FGLGVEALAA HAAAQGITLV YHHHMGTIVE TEDEIDRLMR FTGPHTRLLL DTGHCLFGRG NPERVAQKHI ARVGHIHAKN IRRDIVQDVR DQNLSFLEGV RRGVFTVPGD TEGSVDFVPV LKIAAEHGYQ GWLVIEAEQD PDVRNPFEYQ SLGLKSLKAF AREAGLDKGV
Validated NT Sequence
ttattaaact cccttgtcga gtcctgcctc gcgcgcgaaa gccttcaggg atttcagccc aagcgactga tattcaaaag gattgcgcac atctggatct tgttcggctt cgataacgag ccatccctga tagccgtgct ctgcggcaat tttcagtacg ggcacgaaat ctaccgagcc ttccgtatca cccggtacgg tgaacacacc gcgccgcaca ccttcaagga aggacaggtt ctggtcgcgc acgtcctgca ctatatcgcg gcgaatattc ttggcatgga tatggccgac gcgcgctata tgcttttgcg ccacccgttc tggattgccg cgtccgaaca ggcaatggcc cgtatcgagc aaaagcctgg tgtgcggccc ggtgaaacgc atcagacggt cgatctcgtc ttccgtttcc acgattgtac ccatgtggtg atgatagacg agcgtgatgc cttgcgcggc ggcatgtgcg gccagtgctt ccacaccaag gccgaaggct tcccagcggg catcttccag aacagggcgc tcttgcagcg gcttgtcatt gtcgccatga atggcgttcg aggtttcgca gacgatgata actttcgagc ccatcgcctt caggagatca agcgcgggct gcatcgccgc cttttcttcc tcgacggaat tgaccagcag attgagcgaa tgccagccgg aaacatagcg caacccgcgc ggttccagca ctgctttaag agcggcagct tcctgcggga atttatgacc tttttcgatc ccgtcgaagc cgatttttgc ggcttcgtcg aggcattgat caagcgtgat atgcgcgccg aggctgcggt cgtcatcgtt tgaccatgca atggggttgg tgccgtaaag gatcatcgaa ccaggaccct gggtctgagc ttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILYGTNPI AWSNDDDRSL GAHITLDQCL DEAAKIGFDG IEKGHKFPQE AAALKAVLEP RGLRYVSGWH SLNLLVNSVE EEKAAMQPAL DLLKAMGSKV IIVCETSNAI HGDNDKPLQE RPVLEDARWE AFGLGVEALA AHAAAQGITL VYHHHMGTIV ETEDEIDRLM RFTGPHTRLL LDTGHCLFGR GNPERVAQKH IARVGHIHAK NIRRDIVQDV RDQNLSFLEG VRRGVFTVPG DTEGSVDFVP VLKIAAEHGY QGWLVIEAEQ DPDVRNPFEY QSLGLKSLKA FAREAGLDKG V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcctttac ggcaccaacc ccattgcatg gtcaaacgat gacgaccgca gcctcggcgc gcatatcacg cttgatcaat gcctcgacga agccgcaaaa atcggcttcg acgggatcga aaaaggtcat aaattcccgc aggaagctgc cgctcttaaa gcagtgctgg aaccgcgcgg gttgcgctat gtttccggct ggcattcgct caatctgctg gtcaattccg tcgaggaaga aaaggcggcg atgcagcccg cgcttgatct cctgaaggcg atgggctcga aagttatcat cgtctgcgaa acctcgaacg ccattcatgg cgacaatgac aagccgctgc aagagcgccc tgttctggaa gatgcccgct gggaagcctt cggccttggt gtggaagcac tggccgcaca tgccgccgcg caaggcatca cgctcgtcta tcatcaccac atgggtacaa tcgtggaaac ggaagacgag atcgaccgtc tgatgcgttt caccgggccg cacaccaggc ttttgctcga tacgggccat tgcctgttcg gacgcggcaa tccagaacgg gtggcgcaaa agcatatagc gcgcgtcggc catatccatg ccaagaatat tcgccgcgat atagtgcagg acgtgcgcga ccagaacctg tccttccttg aaggtgtgcg gcgcggtgtg ttcaccgtac cgggtgatac ggaaggctcg gtagatttcg tgcccgtact gaaaattgcc gcagagcacg gctatcaggg atggctcgtt atcgaagccg aacaagatcc agatgtgcgc aatccttttg aatatcagtc gcttgggctg aaatccctga aggctttcgc gcgcgaggca ggactcgaca agggagttta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrmeA.18154.a.A1.PS01279
| PURIFICATION DATe: | 11/17/2011 |
| CONCENTRATION: | 67.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MILYGTNPIA WSNDDDRSLG AHITLDQCLD EAAKIGFDGI EKGHKFPQEA AALKAVLEPR GLRYVSGWHS LNLLVNSVEE EKAAMQPALD LLKAMGSKVI IVCETSNAIH GDNDKPLQER PVLEDARWEA FGLGVEALAA HAAAQGITLV YHHHMGTIVE TEDEIDRLMR FTGPHTRLLL DTGHCLFGRG NPERVAQKHI ARVGHIHAKN IRRDIVQDVR DQNLSFLEGV RRGVFTVPGD TEGSVDFVPV LKIAAEHGYQ GWLVIEAEQD PDVRNPFEYQ SLGLKSLKAF AREAGLDKGV
Validated NT Sequence
ttattaaact cccttgtcga gtcctgcctc gcgcgcgaaa gccttcaggg atttcagccc aagcgactga tattcaaaag gattgcgcac atctggatct tgttcggctt cgataacgag ccatccctga tagccgtgct ctgcggcaat tttcagtacg ggcacgaaat ctaccgagcc ttccgtatca cccggtacgg tgaacacacc gcgccgcaca ccttcaagga aggacaggtt ctggtcgcgc acgtcctgca ctatatcgcg gcgaatattc ttggcatgga tatggccgac gcgcgctata tgcttttgcg ccacccgttc tggattgccg cgtccgaaca ggcaatggcc cgtatcgagc aaaagcctgg tgtgcggccc ggtgaaacgc atcagacggt cgatctcgtc ttccgtttcc acgattgtac ccatgtggtg atgatagacg agcgtgatgc cttgcgcggc ggcatgtgcg gccagtgctt ccacaccaag gccgaaggct tcccagcggg catcttccag aacagggcgc tcttgcagcg gcttgtcatt gtcgccatga atggcgttcg aggtttcgca gacgatgata actttcgagc ccatcgcctt caggagatca agcgcgggct gcatcgccgc cttttcttcc tcgacggaat tgaccagcag attgagcgaa tgccagccgg aaacatagcg caacccgcgc ggttccagca ctgctttaag agcggcagct tcctgcggga atttatgacc tttttcgatc ccgtcgaagc cgatttttgc ggcttcgtcg aggcattgat caagcgtgat atgcgcgccg aggctgcggt cgtcatcgtt tgaccatgca atggggttgg tgccgtaaag gatcatcgaa ccaggaccct gggtctgagc ttccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILYGTNPI AWSNDDDRSL GAHITLDQCL DEAAKIGFDG IEKGHKFPQE AAALKAVLEP RGLRYVSGWH SLNLLVNSVE EEKAAMQPAL DLLKAMGSKV IIVCETSNAI HGDNDKPLQE RPVLEDARWE AFGLGVEALA AHAAAQGITL VYHHHMGTIV ETEDEIDRLM RFTGPHTRLL LDTGHCLFGR GNPERVAQKH IARVGHIHAK NIRRDIVQDV RDQNLSFLEG VRRGVFTVPG DTEGSVDFVP VLKIAAEHGY QGWLVIEAEQ DPDVRNPFEY QSLGLKSLKA FAREAGLDKG V
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCCTTTAC GGCACCAACC CCATTGCATG GTCAAACGAT GACGACCGCA GCCTCGGCGC GCATATCACG CTTGATCAAT GCCTCGACGA AGCCGCAAAA ATCGGCTTCG ACGGGATCGA AAAAGGTCAT AAATTCCCGC AGGAAGCTGC CGCTCTTAAA GCAGTGCTGG AACCGCGCGG GTTGCGCTAT GTTTCCGGCT GGCATTCGCT CAATCTGCTG GTCAATTCCG TCGAGGAAGA AAAGGCGGCG ATGCAGCCCG CGCTTGATCT CCTGAAGGCG ATGGGCTCGA AAGTTATCAT CGTCTGCGAA ACCTCGAACG CCATTCATGG CGACAATGAC AAGCCGCTGC AAGAGCGCCC TGTTCTGGAA GATGCCCGCT GGGAAGCCTT CGGCCTTGGT GTGGAAGCAC TGGCCGCACA TGCCGCCGCG CAAGGCATCA CGCTCGTCTA TCATCACCAC ATGGGTACAA TCGTGGAAAC GGAAGACGAG ATCGACCGTC TGATGCGTTT CACCGGGCCG CACACCAGGC TTTTGCTCGA TACGGGCCAT TGCCTGTTCG GACGCGGCAA TCCAGAACGG GTGGCGCAAA AGCATATAGC GCGCGTCGGC CATATCCATG CCAAGAATAT TCGCCGCGAT ATAGTGCAGG ACGTGCGCGA CCAGAACCTG TCCTTCCTTG AAGGTGTGCG GCGCGGTGTG TTCACCGTAC CGGGTGATAC GGAAGGCTCG GTAGATTTCG TGCCCGTACT GAAAATTGCC GCAGAGCACG GCTATCAGGG ATGGCTCGTT ATCGAAGCCG AACAAGATCC AGATGTGCGC AATCCTTTTG AATATCAGTC GCTTGGGCTG AAATCCCTGA AGGCTTTCGC GCGCGAGGCA GGACTCGACA AGGGAGTTTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A