BrmeA.18144.b

Cobalamin synthesis protein/P47K family protein

CENTER ID: BrmeA.18144.b
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18144.b.B1.GE36713 Full length( BrmeA.18144.b ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_0036
BV-BRC: fig|224914.11.peg.36
RefSeq: NP_538954.1
UniProt: Q8YJP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDPIPVSVL TGFLGSGKTT LLNRLLKDPA LSDTAVIINE FGEVSIDHLL VEQASEGVIE LADGCLCCTV RGELVDTLAD LIDRLQTGRI KALKRVIIET TGLADPAPVL HSIMGHPLLT QVFRLDGVLA TVDAVNGMAT LDNHEEAVKQ AAMADRIILT KTDLPEAQAG LPALKARLRT LNPGADILEA GEERTGYAAL FECGLYNPQT KSADVRRWLK AEAYEDEHHG HVCGPDCGHD HHHHDHHHDH HNDQGYAHHH HHDDAIRSFS LRHDAPIPVS TFEMFLDLLR STHGEKLLRM KGIVQIAEDP DRPVVIHGVQ KIFHPPARLP QWPQGKRETL LVLIVKDLPE AYVRELFDAF LGRPGLDRPD RAAMTENPLA IPGFSPRR
NT Sequence
ATGCCTGATC CCATTCCCGT TTCCGTGCTG ACCGGCTTTC TTGGCTCCGG CAAGACGACG CTGCTCAACC GGCTTTTGAA AGATCCGGCG CTCAGCGATA CAGCAGTCAT TATCAACGAG TTCGGCGAGG TGAGCATTGA CCATCTGCTG GTCGAGCAGG CGAGCGAGGG CGTGATCGAG CTGGCTGACG GGTGTCTGTG CTGCACAGTG CGCGGTGAAC TGGTCGATAC GCTGGCCGAT CTCATCGACC GGTTGCAGAC CGGCCGCATC AAGGCGCTGA AGCGCGTCAT CATCGAAACC ACGGGGCTTG CCGATCCGGC CCCGGTGCTG CATTCGATCA TGGGTCATCC TCTTCTCACG CAGGTTTTCC GGCTTGATGG CGTGCTGGCT ACCGTGGATG CGGTGAACGG CATGGCAACG CTCGACAATC ATGAGGAAGC CGTCAAACAG GCAGCCATGG CCGACCGCAT CATCCTCACC AAGACCGATC TGCCGGAAGC ACAGGCTGGC TTGCCGGCAT TGAAGGCACG GTTGCGCACG CTTAACCCCG GAGCCGATAT TCTGGAAGCG GGGGAAGAGC GCACAGGCTA TGCCGCGCTT TTTGAATGCG GGCTTTATAA CCCGCAGACG AAATCGGCGG ATGTGCGGCG CTGGCTTAAG GCCGAAGCCT ATGAGGACGA GCATCATGGC CATGTGTGCG GGCCGGATTG CGGTCACGAC CATCATCACC ACGATCACCA TCACGATCAC CATAACGATC AGGGCTATGC GCATCATCAT CATCATGACG ATGCGATCCG TTCGTTTTCA CTGCGCCATG ATGCGCCGAT CCCGGTTTCG ACCTTCGAGA TGTTTCTCGA TCTTCTGCGT TCGACCCATG GTGAAAAGCT CTTGCGCATG AAGGGTATCG TGCAGATTGC CGAAGACCCG GACCGCCCGG TCGTCATCCA TGGTGTGCAG AAGATTTTCC ATCCGCCCGC GCGCCTGCCG CAATGGCCGC AAGGCAAGCG CGAAACGCTT CTGGTCCTGA TTGTGAAGGA CTTGCCGGAG GCCTATGTGC GGGAACTTTT CGATGCCTTT CTGGGCCGGC CCGGCCTCGA CAGGCCCGAC CGCGCAGCCA TGACTGAAAA TCCGCTGGCC ATTCCGGGGT TCTCACCCCG CCGG
Details for BrmeA.18144.b.B1.GE36713
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 67
Validated AA Sequence
MGHPLLTQVF RLDGVLATVD AVNGMATLDN HEEAVKQAAM ADRIILTKTD LPEAQAGLPA LKARLRTLNP GADILEAGEE RTGYAALFEC GLYNPQTKSA DVRRWLKAEA YEDEHHGHVC GPDCGHDHHH HDHHHDHHND QGYAHHHHHD DAIRSFSLRH DAPIPVSTFE MFLDLLRSTH GEKLLRMKGI VQIAEDPDRP VVIHGVQKIF HPPARLPQWP QGKRETLLVL IVKDLPEAYV RELFDAFLGR PGLDRPDRAA MTENPLA
Validated NT Sequence
tggccagcgg attttcagtc atggctgcgc ggtcgggcct gtcgaggccg ggccggccca gaaaggcatc gaaaagttcc cgcacatagg cctccggcaa gtccttcaca atcaggacca gaagcgtttc gcgcttgcct tgcggccatt gcggcaggcg cgcgggcgga tggaaaatct tctgcacacc atggatgacg accgggcggt ccgggtcttc ggcaatctgc acgataccct tcatgcgcaa gagcttttca ccatgggtcg aacgcagaag atcgagaaac atctcgaagg tcgaaaccgg gatcggcgca tcatggcgca gtgaaaacga acggatcgca tcgtcatgat gatgatgatg cgcatagccc tgatcgttat ggtgatcgtg atggtgatcg tggtgatgat ggtcgtgacc gcaatccggc ccgcacacat ggccatgatg ctcgtcctca taggcttcgg ccttaagcca gcgccgcaca tccgccgatt tcgtctgcgg gttataaagc ccgcattcaa aaagcgcggc atagcctgtg cgctcttccc ccgcttccag aatatcggct ccggggttaa gcgtgcgcaa ccgtgccttc aatgccggca agccagcctg tgcttccggc agatcggtct tggtgaggat gatgcggtcg gccatggctg cctgtttgac ggcttcctca tgattgtcga gcgttgccat gccgttcacc gcatccacgg tagccagcac gccatcaagc cggaaaacct gcgtgagaag aggatgaccc atgatcgaat gcagcaccgg ggccggatcg gcaagccccg tggtttcgat gatgacgcgc ttcagcgcct tgatgcggcc ggtctgcaac cggtcgatga gatcggccag cgtatcgacc agttcaccgc gcactgtgca gcacagacac ccgtcagcca gctcgatcac gccctcgctc gcctgctcga ccagcagatg gtcaatgctc acctcgccga actcgttgat aatgactgct gtatcgctga gcgccggatc tttcaaaagc cggttgagca gcgtcgtctt gccggagcca agaaagccgg tcagcacgga aacgggaatg gga
Expected Protein Sequence
MAHHHHHHMP DPIPVSVLTG FLGSGKTTLL NRLLKDPALS DTAVIINEFG EVSIDHLLVE QASEGVIELA DGCLCCTVRG ELVDTLADLI DRLQTGRIKA LKRVIIETTG LADPAPVLHS IMGHPLLTQV FRLDGVLATV DAVNGMATLD NHEEAVKQAA MADRIILTKT DLPEAQAGLP ALKARLRTLN PGADILEAGE ERTGYAALFE CGLYNPQTKS ADVRRWLKAE AYEDEHHGHV CGPDCGHDHH HHDHHHDHHN DQGYAHHHHH DDAIRSFSLR HDAPIPVSTF EMFLDLLRST HGEKLLRMKG IVQIAEDPDR PVVIHGVQKI FHPPARLPQW PQGKRETLLV LIVKDLPEAY VRELFDAFLG RPGLDRPDRA AMTENPLAIP GFSPRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg atcccattcc cgtttccgtg ctgaccggct ttcttggctc cggcaagacg acgctgctca accggctttt gaaagatccg gcgctcagcg atacagcagt cattatcaac gagttcggcg aggtgagcat tgaccatctg ctggtcgagc aggcgagcga gggcgtgatc gagctggctg acgggtgtct gtgctgcaca gtgcgcggtg aactggtcga tacgctggcc gatctcatcg accggttgca gaccggccgc atcaaggcgc tgaagcgcgt catcatcgaa accacggggc ttgccgatcc ggccccggtg ctgcattcga tcatgggtca tcctcttctc acgcaggttt tccggcttga tggcgtgctg gctaccgtgg atgcggtgaa cggcatggca acgctcgaca atcatgagga agccgtcaaa caggcagcca tggccgaccg catcatcctc accaagaccg atctgccgga agcacaggct ggcttgccgg cattgaaggc acggttgcgc acgcttaacc ccggagccga tattctggaa gcgggggaag agcgcacagg ctatgccgcg ctttttgaat gcgggcttta taacccgcag acgaaatcgg cggatgtgcg gcgctggctt aaggccgaag cctatgagga cgagcatcat ggccatgtgt gcgggccgga ttgcggtcac gaccatcatc accacgatca ccatcacgat caccataacg atcagggcta tgcgcatcat catcatcatg acgatgcgat ccgttcgttt tcactgcgcc atgatgcgcc gatcccggtt tcgaccttcg agatgtttct cgatcttctg cgttcgaccc atggtgaaaa gctcttgcgc atgaagggta tcgtgcagat tgccgaagac ccggaccgcc cggtcgtcat ccatggtgtg cagaagattt tccatccgcc cgcgcgcctg ccgcaatggc cgcaaggcaa gcgcgaaacg cttctggtcc tgattgtgaa ggacttgccg gaggcctatg tgcgggaact tttcgatgcc tttctgggcc ggcccggcct cgacaggccc gaccgcgcag ccatgactga aaatccgctg gccattccgg ggttctcacc ccgccggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at