SSGCID: Target Page

BrmeA.18143.a

Phosphoglucomutase (EC 5.4.2.2)

CENTER ID:	BrmeA.18143.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18143.a.A1.GE33811	full length		1	566

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18143.a.A1.PW34421	full length		1	566

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.2084
RefSeq:	NP_540803.1
UniProt:	Q8YEJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSCNDRKHRQ GARCQPIETN GNNMTVKTIA TTPYQDQQPG TSGLRKKVPV FQQPNYAENF IQSIFDVLEG FKGKTLVVGG DGRFYNREVI QKLIKIAAAN GFGRIMVGQG GILSTPAASN MIRKYKAFGG MILSASHNPG GPTQDFGIKY NIGNGGPAPE KITEAIFARS KVIDQYKIAD AADIDIDKIG TSKIGDTEVV IFDPVADYAE LMESLFDFAA IRAMIKGGFQ MKFDAMHAVT GPYAKEIFER HLGAPEGSVV NFVPLPDFGG HHPDPNLVYA KDLYDLLMSS HAPDFGAASD GDGDRNLILG RGIFITPSDS LAMLAANAHL APGYKGGIKG IARSMPTSAA ADRVAEKLGI GMYETPTGWK FFGNLLDSGK VTICGEESSG TGSDHVREKD GLWAVLLWLN ILAVRKESVK AIADDHWARF GRNYYTRHDY EAVDSDIATK LVADLRGKLA GLPGTSVNGL RIEKADDFAY HDPVDGSTSE HQGIRIYFEG GARIVLRLSG TGTSGATIRI YIERYEADPA KHNLDTQATL APLIDAAEQI AEVKKRSGRT EPSVVT

NT Sequence

ttgtcttgca atgaccgcaa acaccgacaa ggtgcacggt gccaaccgat tgaaacgaac gggaacaaca tgaccgtcaa gaccattgcg accacgccat accaggatca acagccgggc acttcgggct tgcgcaagaa ggttccggtt ttccagcagc ccaattatgc agaaaatttc atccagtcga tcttcgacgt gctggagggg ttcaagggca agacgctcgt ggttggcggc gacggacgct tttataaccg cgaggtgatc cagaagctta tcaagatcgc agccgccaac ggttttggcc gtatcatggt gggacagggc ggcattcttt ccacgccagc cgcctccaac atgatccgca agtacaaagc cttcggtggc atgatccttt cggccagcca caatccgggc gggcctacgc aggatttcgg catcaagtac aatatcggca atggtggacc ggcacccgaa aagatcaccg aagcgatctt cgcccgctca aaggtgatcg accagtataa aattgcagat gcagccgata tcgatatcga caagatcggc acaagcaaga tcggcgatac cgaggtggtc atcttcgatc cggtggccga ttatgctgaa ttgatggaaa gcctgttcga tttcgccgcc atccgcgcca tgataaaagg cggcttccag atgaagttcg acgccatgca tgcggtgacc gggccttatg ccaaggaaat cttcgagcgc cacctcggtg cgcccgaagg cagcgtcgtc aatttcgtgc ccctgccgga tttcggtggc catcacccgg atccaaacct cgtctatgcc aaggatctct acgatcttct gatgtccagc catgcgccgg attttggcgc cgcctccgac ggcgatggcg atcgcaacct cattctcggg cgcggtatct tcatcacgcc gtcagattcg cttgccatgc tggccgcaaa tgcccatctg gcgccgggct ataaaggcgg tatcaagggc attgcccgtt ccatgccgac gagcgccgcc gccgaccgcg tggcggaaaa actcggtatc ggcatgtatg aaaccccgac cggctggaaa ttcttcggca atcttctcga cagcggcaag gtgacgatct gcggcgagga aagctccggc accggctccg accatgtgcg cgaaaaagat ggtttgtggg ccgttctgct ctggctcaac attctggcgg tgcgcaagga aagtgtgaag gcaatcgcgg acgaccattg ggcacgtttc gggcgcaatt attacactcg ccacgactat gaagccgtcg attccgatat tgcgacgaag ctggtggctg acctgcgcgg caagcttgcc ggcctgcccg gcacgagcgt caatggcctc aggatcgaaa aggccgacga cttcgcctat catgatcccg ttgatggctc gaccagcgaa catcagggca tccgcatcta ttttgaagga ggtgcgcgca tcgtgcttcg cctgtccggt accgggacgt caggcgcgac catccgcatc tatatcgaac gctatgaagc cgatcctgca aaacataatc tcgacacgca ggcaacgctg gccccactga tcgacgcggc tgaacagatc gccgaagtaa aaaaacgcag cgggcggaca gagccgtcgg tggttacttg a

Details for BrmeA.18143.a.A1.GE33811

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	96

Validated AA Sequence

MLSCNDRKHR QGARCQPIET NGNNMTVKTI ATTPYQDQQP GTSGLRKKVP VFQQPNYAEN FIQSIFDVLE GFKGKTLVVG GDGRFYNREV IQKLIKIAAA NGFGRIMVGQ GGILSTPAAS NMIRKYKAFG GMILSASHNP GGPTQDFGIK YNIGNGGPAP EKITEAIFAR SKVIDQYKIA DAADIDIDKI GTSKIGDTEV VIFDPVADYA ELMESLFXFX AXRAMIXXGF QMKFDAXHAV XGPYAKEIFX RHLXAPEGSV VNFVPLPXFG GHHPDPNLVY AKDLYXLLMX SHAPDFGAAS DGDGDRNLIX GRGIFITPSX XLAMLAANAH LAPGYKGGIK GIARSMPTSA AADRVAEKLG IGMYETPTGW KFFGNLLDSG KVTICGEESS GTGSDHVREK DGLWAVLLWL NILAVRKESV KAIADDHWAR FGRNYYTRHD YEAVDSDIAT KLVADLRGKL AGLPGTSVNG LRIEKADDFA YHDPVDGSTS EHQGIRIYFE GGARIVLRLS GTGTSGATIR IYIERYEADP AKHNLDTQAT LAPLIDAAEQ IAEVKKRSGR TEPSVVT

Validated NT Sequence

attaagtaac caccgacggc tctgtccgcc cgctgcgttt ttttacttcg gcgatctgtt cagccgcgtc gatcagtggg gccagcgttg cctgcgtgtc gagattatgt tttgcaggat cggcttcata gcgttcgata tagatgcgga tggtcgcgcc tgacgtcccg gtaccggaca ggcgaagcac gatgcgcgca cctccttcaa aatagatgcg gatgccctga tgttcgctgg tcgagccatc aacgggatca tgataggcga agtcgtcggc cttttcgatc ctgaggccat tgacgctcgt gccgggcagg ccggcaagct tgccgcgcag gtcagccacc agcttcgtcg caatatcgga atcgacggct tcatagtcgt ggcgagtgta ataattgcgc ccgaaacgtg cccaatggtc gtccgcgatt gccttcacac tttccttgcg caccgccaga atgttgagcc agagcagaac ggcccacaaa ccatcttttt cgcgcacatg gtcggagccg gtgccggagc tttcctcgcc gcagatcgtc accttgccgc tgtcgagaag attgccgaag aatttccagc cggtcggggt ttcatacatg ccgataccga gtttttccgc cacgcggtcg gcggcggcgc tcgtcggcat ggaacgggca atgcccttga taccgccttt atagcccggc gccagatggg catttgcggc cagcatggca agcgnntctg acggcgtgat gaagataccg cgcccganaa tgaggttgcg atcgccatcg ccgtcggang cggcgccaaa atccggcgca tggctggnca tcagaaganc gtagagatcc ttggcataga cgaggtttgg atcngggtga tgnccnccga antccggcag gggcacgaaa ttgacgacgc tgccttcggg cgcacngagg tggcgctnga agatttcctt ggcataaggc ccgntcaccg catgcanggc gtcgaacttc atctggaagc cgcnntttat catggcgcgg anggcggnga antcgaacag gctttccatc aattcagcat aatcggccac cggatcgaag atgaccacct cggtatcgcc gatcttgctt gtgccgatct tgtcgatatc gatatcggct gcatctgcaa ttttatactg gtcgatcacc tttgagcggg cgaagatcgc ttcggtgatc ttttcgggtg ccggtccacc attgccgata ttgtacttga tgccgaaatc ctgcgtaggc ccgcccggat tgtggctggc cgaaaggatc atgccaccga aggctttgta cttgcggatc atgttggagg cggctggcgt ggaaagaatg ccgccctgtc ccaccatgat acggccaaaa ccgttggcgg ctgcgatctt gataagcttc tggatcacct cgcggttata aaagcgtccg tcgccgccaa ccacgagcgt cttgcccttg aacccctcca gcacgtcgaa gatcgactgg atgaaatttt ctgcataatt gggctgctgg aaaaccggaa ccttcttgcg caagcccgaa gtgcccggct gttgatcctg gtatggcgtg gtcgcaatgg tcttgacggt catgttgttc ccgttcgttt caatcggttg gcaccgtgca ccttgtcggt gtttgcggtc attgcaagac aacatcgaac caggaccctg ggtctgagct tccagggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLSCNDRKH RQGARCQPIE TNGNNMTVKT IATTPYQDQQ PGTSGLRKKV PVFQQPNYAE NFIQSIFDVL EGFKGKTLVV GGDGRFYNRE VIQKLIKIAA ANGFGRIMVG QGGILSTPAA SNMIRKYKAF GGMILSASHN PGGPTQDFGI KYNIGNGGPA PEKITEAIFA RSKVIDQYKI ADAADIDIDK IGTSKIGDTE VVIFDPVADY AELMESLFDF AAIRAMIKGG FQMKFDAMHA VTGPYAKEIF ERHLGAPEGS VVNFVPLPDF GGHHPDPNLV YAKDLYDLLM SSHAPDFGAA SDGDGDRNLI LGRGIFITPS DSLAMLAANA HLAPGYKGGI KGIARSMPTS AAADRVAEKL GIGMYETPTG WKFFGNLLDS GKVTICGEES SGTGSDHVRE KDGLWAVLLW LNILAVRKES VKAIADDHWA RFGRNYYTRH DYEAVDSDIA TKLVADLRGK LAGLPGTSVN GLRIEKADDF AYHDPVDGST SEHQGIRIYF EGGARIVLRL SGTGTSGATI RIYIERYEAD PAKHNLDTQA TLAPLIDAAE QIAEVKKRSG RTEPSVVT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtcttgc aatgaccgca aacaccgaca aggtgcacgg tgccaaccga ttgaaacgaa cgggaacaac atgaccgtca agaccattgc gaccacgcca taccaggatc aacagccggg cacttcgggc ttgcgcaaga aggttccggt tttccagcag cccaattatg cagaaaattt catccagtcg atcttcgacg tgctggaggg gttcaagggc aagacgctcg tggttggcgg cgacggacgc ttttataacc gcgaggtgat ccagaagctt atcaagatcg cagccgccaa cggttttggc cgtatcatgg tgggacaggg cggcattctt tccacgccag ccgcctccaa catgatccgc aagtacaaag ccttcggtgg catgatcctt tcggccagcc acaatccggg cgggcctacg caggatttcg gcatcaagta caatatcggc aatggtggac cggcacccga aaagatcacc gaagcgatct tcgcccgctc aaaggtgatc gaccagtata aaattgcaga tgcagccgat atcgatatcg acaagatcgg cacaagcaag atcggcgata ccgaggtggt catcttcgat ccggtggccg attatgctga attgatggaa agcctgttcg atttcgccgc catccgcgcc atgataaaag gcggcttcca gatgaagttc gacgccatgc atgcggtgac cgggccttat gccaaggaaa tcttcgagcg ccacctcggt gcgcccgaag gcagcgtcgt caatttcgtg cccctgccgg atttcggtgg ccatcacccg gatccaaacc tcgtctatgc caaggatctc tacgatcttc tgatgtccag ccatgcgccg gattttggcg ccgcctccga cggcgatggc gatcgcaacc tcattctcgg gcgcggtatc ttcatcacgc cgtcagattc gcttgccatg ctggccgcaa atgcccatct ggcgccgggc tataaaggcg gtatcaaggg cattgcccgt tccatgccga cgagcgccgc cgccgaccgc gtggcggaaa aactcggtat cggcatgtat gaaaccccga ccggctggaa attcttcggc aatcttctcg acagcggcaa ggtgacgatc tgcggcgagg aaagctccgg caccggctcc gaccatgtgc gcgaaaaaga tggtttgtgg gccgttctgc tctggctcaa cattctggcg gtgcgcaagg aaagtgtgaa ggcaatcgcg gacgaccatt gggcacgttt cgggcgcaat tattacactc gccacgacta tgaagccgtc gattccgata ttgcgacgaa gctggtggct gacctgcgcg gcaagcttgc cggcctgccc ggcacgagcg tcaatggcct caggatcgaa aaggccgacg acttcgccta tcatgatccc gttgatggct cgaccagcga acatcagggc atccgcatct attttgaagg aggtgcgcgc atcgtgcttc gcctgtccgg taccgggacg tcaggcgcga ccatccgcat ctatatcgaa cgctatgaag ccgatcctgc aaaacataat ctcgacacgc aggcaacgct ggccccactg atcgacgcgg ctgaacagat cgccgaagta aaaaaacgca gcgggcggac agagccgtcg gtggttactt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BrmeA.18143.a.A1.PW34421

PURIFICATION DATe:	12/2/2011
CONCENTRATION:	27.35mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTCTTGC AATGACCGCA AACACCGACA AGGTGCACGG TGCCAACCGA TTGAAACGAA CGGGAACAAC ATGACCGTCA AGACCATTGC GACCACGCCA TACCAGGATC AACAGCCGGG CACTTCGGGC TTGCGCAAGA AGGTTCCGGT TTTCCAGCAG CCCAATTATG CAGAAAATTT CATCCAGTCG ATCTTCGACG TGCTGGAGGG GTTCAAGGGC AAGACGCTCG TGGTTGGCGG CGACGGACGC TTTTATAACC GCGAGGTGAT CCAGAAGCTT ATCAAGATCG CAGCCGCCAA CGGTTTTGGC CGTATCATGG TGGGACAGGG CGGCATTCTT TCCACGCCAG CCGCCTCCAA CATGATCCGC AAGTACAAAG CCTTCGGTGG CATGATCCTT TCGGCCAGCC ACAATCCGGG CGGGCCTACG CAGGATTTCG GCATCAAGTA CAATATCGGC AATGGTGGAC CGGCACCCGA AAAGATCACC GAAGCGATCT TCGCCCGCTC AAAGGTGATC GACCAGTATA AAATTGCAGA TGCAGCCGAT ATCGATATCG ACAAGATCGG CACAAGCAAG ATCGGCGATA CCGAGGTGGT CATCTTCGAT CCGGTGGCCG ATTATGCTGA ATTGATGGAA AGCCTGTTCG ATTTCGCCGC CATCCGCGCC ATGATAAAAG GCGGCTTCCA GATGAAGTTC GACGCCATGC ATGCGGTGAC CGGGCCTTAT GCCAAGGAAA TCTTCGAGCG CCACCTCGGT GCGCCCGAAG GCAGCGTCGT CAATTTCGTG CCCCTGCCGG ATTTCGGTGG CCATCACCCG GATCCAAACC TCGTCTATGC CAAGGATCTC TACGATCTTC TGATGTCCAG CCATGCGCCG GATTTTGGCG CCGCCTCCGA CGGCGATGGC GATCGCAACC TCATTCTCGG GCGCGGTATC TTCATCACGC CGTCAGATTC GCTTGCCATG CTGGCCGCAA ATGCCCATCT GGCGCCGGGC TATAAAGGCG GTATCAAGGG CATTGCCCGT TCCATGCCGA CGAGCGCCGC CGCCGACCGC GTGGCGGAAA AACTCGGTAT CGGCATGTAT GAAACCCCGA CCGGCTGGAA ATTCTTCGGC AATCTTCTCG ACAGCGGCAA GGTGACGATC TGCGGCGAGG AAAGCTCCGG CACCGGCTCC GACCATGTGC GCGAAAAAGA TGGTTTGTGG GCCGTTCTGC TCTGGCTCAA CATTCTGGCG GTGCGCAAGG AAAGTGTGAA GGCAATCGCG GACGACCATT GGGCACGTTT CGGGCGCAAT TATTACACTC GCCACGACTA TGAAGCCGTC GATTCCGATA TTGCGACGAA GCTGGTGGCT GACCTGCGCG GCAAGCTTGC CGGCCTGCCC GGCACGAGCG TCAATGGCCT CAGGATCGAA AAGGCCGACG ACTTCGCCTA TCATGATCCC GTTGATGGCT CGACCAGCGA ACATCAGGGC ATCCGCATCT ATTTTGAAGG AGGTGCGCGC ATCGTGCTTC GCCTGTCCGG TACCGGGACG TCAGGCGCGA CCATCCGCAT CTATATCGAA CGCTATGAAG CCGATCCTGC AAAACATAAT CTCGACACGC AGGCAACGCT GGCCCCACTG ATCGACGCGG CTGAACAGAT CGCCGAAGTA AAAAAACGCA GCGGGCGGAC AGAGCCGTCG GTGGTTACTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA