SSGCID: Target Page

BrmeA.18138.a

C-di-gmp phosphodiesterase a-related protein

CENTER ID:	BrmeA.18138.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18138.a.A2.GE33789	domain1		1	231
BrmeA.18138.a.A3.GE33803	domain2		253	526

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18138.a.A3.PS01269	domain2		253	526

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.1614
RefSeq:	NP_540365.1
UniProt:	Q8YFS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLILGAVAGQ LVGKGLRILE EERHMDIYMT ALRDYSSRLL ASARNTLEAA NNSPFEICSP QDLAYQRKLV FAAYHIKDIG RLRDDQLICS TLLSDIKTQP RRSVANDNLK DGTYIYADDA LITPGSHGPI IGRGNANVVL SSVAFDLLHT PRYAFSIFAT DEEKKHFALL YNYSGNAEPG PEPSSGDWAQ KFVEAPDGKV LRENSCDPDT GVCISLSATI DRTSTAARLK NLLAICLGAL AGGSVGLGWL YYRNRDHSLL YRLNKALAAG EPHLLYQPVV DMADGKVLGF EALIRWEIRK GDFVPPDVFV ARAEEAGTAT KITIYVLERL IEDMGDTLRQ RRDLRININI TASDLQSPAF MSRMERRLAA ADIEPQQVGL ELTERTAVDF SKATDGIRRL REQGHRIYID DFGTGYSSLA YLGELHVDAI KIDKAFTRTV GNDYATVSII PQIISMAKEH GLDIVVEGVE TEAQVLYFRK LGTPLAAQGW FFGKPLCVSD AQALVATVKK KQSRVKLARK TTQKAK

NT Sequence

atgcttattc tgggggccgt ggccgggcaa ctggtcggaa aaggactccg cattctcgaa gaagaacgtc atatggatat ctatatgacg gctctgcgcg attactccag ccggctgctc gcctccgcga ggaatactct ggaggctgcg aataattctc ccttcgagat atgttccccg caggatcttg cctatcagcg caagctcgtc tttgccgcct atcacatcaa ggatatcggg cgtctgcgcg atgaccaact tatctgctcg acgcttctga gtgatatcaa aactcagccg cgccgttccg ttgccaatga caatttgaag gacggaacct atatctatgc cgatgatgcc ttgataacgc cgggtagcca tgggccgatc atcggacgag ggaatgcgaa tgtggtcttg agctcggttg ccttcgatct cctgcacacg ccgcgctatg ctttttccat ttttgccacc gacgaggaaa agaagcattt cgcgctgctt tacaattatt caggtaatgc cgagcctgga cctgagccgt cctctggtga ttgggcgcag aaattcgtgg aagcgccgga tggcaaggtg ttgcgggaga atagctgtga cccggatacg ggcgtctgca tcagcctgtc agcaacgatc gaccgaacca gcacggcagc acggctgaaa aacctgctgg ccatctgcct tggcgcattg gcgggtggca gtgtcgggct gggctggctc tattatcgca atcgcgacca ctcattgctc tacaggctga acaaggcact cgctgccgga gagccgcatt tgctctacca gccggtggtg gatatggcgg atggcaaggt gctcggcttt gaagcattga tccgatggga aatccgcaag ggcgatttcg tcccgccgga tgtcttcgtt gcgcgggcgg aggaggcggg caccgctaca aagataacga tctatgtgct ggaacgtctg atagaagaca tgggcgacac gctgcgccag cgccgtgatt tgcgtatcaa tatcaacatt accgccagcg acctgcaaag cccggccttc atgagccgga tggaaaggcg ccttgcggcg gctgatatcg aaccacaaca ggtggggttg gaactgacgg aacgcacggc ggtcgatttt tccaaggcga cggacggcat caggcgcctg cgcgagcagg ggcaccggat ttatatcgac gatttcggca ccggctattc gagccttgcc tatctcggcg aattgcatgt ggacgcaatc aagatcgaca aggctttcac gcgcactgtc ggcaatgatt acgcaacggt ctccatcatt ccgcagatca tttccatggc gaaggaacat ggcctcgata tcgtggtcga aggggtggaa acggaggcac aggtcctcta ttttcgtaaa ctgggcacgc ccctggccgc acaaggctgg ttttttggca agccgctgtg cgtttccgat gcgcaggcgc tggtcgctac agtgaagaag aagcaatcgc gcgtgaagtt ggcccgcaaa acgacccaaa aggcgaaatg a

Details for BrmeA.18138.a.A2.GE33789

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLILGAVAG QLVGKGLRIL EEERHMDIYM TALRDYSSRL LASARNTLEA ANNSPFEICS PQDLAYQRKL VFAAYHIKDI GRLRDDQLIC STLLSDIKTQ PRRSVANDNL KDGTYIYADD ALITPGSHGP IIGRGNANVV LSSVAFDLLH TPRYAFSIFA TDEEKKHFAL LYNYSGNAEP GPEPSSGDWA QKFVEAPDGK VLRENSCDPD TGVCISLSAT IDRTSTAARL KN

Validated NT Sequence

attttnttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctta ttctgggggc cgtggccggg caactggtcg gaaaaggact ccgcattctc gaagaagaac gtcatatgga tatctatatg acggctctgc gcgattactc cagccggctg ctcgcctccg cgaggaatac tctggaggct gcgaataatt ctcccttcga gatatgttcc ccgcaggatc ttgcctatca gcgcaagctc gtctttgccg cctatcacat caaggatatc gggcgtctgc gcgatgacca acttatctgc tcgacgcttc tgagtgatat caaaactcag ccgcgccgtt ccgttgccaa tgacaatttg aaggacggaa cctatatcta tgccgatgat gccttgataa cgccgggtag ccatgggccg atcatcggac gagggaatgc gaatgtggtc ttgagctcgg ttgccttcga tctcctgcac acgccgcgct atgctttttc catttttgcc accgacgagg aaaagaagca tttcgcgctg ctttacaatt attcaggtaa tgccgagcct ggacctgagc cgtcctctgg tgattgggcg cagaaattcg tggaagcgcc ggatggcaag gtgttgcggg agaatagctg tgacccggat acgggcgtct gcatcagcct gtcagcaacg atcgaccgaa ccagcacggc agcacggctg aaaaactaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tganttggct gctgcnnnnn nnnnnnnnnn nnnnnnnnnn cccccnnnnn nngnnnnncn ncnnnnnnnn nncnnnnnnn nngnnnnngn nnngnnnncn nttgcnaann nccccnannn nnnnnnnngg nnnnggnngn nnnnnnngnn nngnnnnagg nnnangnnnn cttnnnnnng nccnnnnnnn nnnnncnngt nttnntnncc cnnngnannn ngnnnnnnnn nnncnnnnnn ngnnnnnngg nnnnnnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttattctg ggggccgtgg ccgggcaact ggtcggaaaa ggactccgca ttctcgaaga agaacgtcat atggatatct atatgacggc tctgcgcgat tactccagcc ggctgctcgc ctccgcgagg aatactctgg aggctgcgaa taattctccc ttcgagatat gttccccgca ggatcttgcc tatcagcgca agctcgtctt tgccgcctat cacatcaagg atatcgggcg tctgcgcgat gaccaactta tctgctcgac gcttctgagt gatatcaaaa ctcagccgcg ccgttccgtt gccaatgaca atttgaagga cggaacctat atctatgccg atgatgcctt gataacgccg ggtagccatg ggccgatcat cggacgaggg aatgcgaatg tggtcttgag ctcggttgcc ttcgatctcc tgcacacgcc gcgctatgct ttttccattt ttgccaccga cgaggaaaag aagcatttcg cgctgcttta caattattca ggtaatgccg agcctggacc tgagccgtcc tctggtgatt gggcgcagaa attcgtggaa gcgccggatg gcaaggtgtt gcgggagaat agctgtgacc cggatacggg cgtctgcatc agcctgtcag caacgatcga ccgaaccagc acggcagcac ggctgaaaaa ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BrmeA.18138.a.A3.GE33803

HARVESTED ON:	10/4/2011
SEQUENCED ON:	10/7/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRNRDHSLL YRLNKALAAG EPHLLYQPVV DMADGKVLGF EALIRWEIRK GDFVPPDVFV ARAEEAGTAT KITIYVLERL IEDMGDTLRQ RRDLRININI TASDLQSPAF MSRMERRLAA ADIEPQQVGL ELTERTAVDF SKATDGIRRL REQGHRIYID DFGTGYSSLA YLGELHVDAI KIDKAFTRTV GNDYATVSII PQIISMAKEH GLDIVVEGVE TEAQVLYFRK LGTPLAAQGW FFGKPLCVSD AQALVATVKK KQSRVKLARK TTQKAK

Validated NT Sequence

ttattatttc gccttttggg tcgttttgcg ggccaacttc acgcgcgatt gcttcttctt cactgtagcg accagcgcct gcgcatcgga aacgcacagc ggcttgccaa aaaaccagcc ttgtgcggcc aggggcgtgc ccagtttacg aaaatagagg acctgtgcct ccgtttccac cccttcgacc acgatatcga ggccatgttc cttcgccatg gaaatgatct gcggaatgat ggagaccgtt gcgtaatcat tgccgacagt gcgcgtgaaa gccttgtcga tcttgattgc gtccacatgc aattcgccga gataggcaag gctcgaatag ccggtgccga aatcgtcgat ataaatccgg tgcccctgct cgcgcaggcg cctgatgccg tccgtcgcct tggaaaaatc gaccgccgtg cgttccgtca gttccaaccc cacctgttgt ggttcgatat cagccgccgc aaggcgcctt tccatccggc tcatgaaggc cgggctttgc aggtcgctgg cggtaatgtt gatattgata cgcaaatcac ggcgctggcg cagcgtgtcg cccatgtctt ctatcagacg ttccagcaca tagatcgtta tctttgtagc ggtgcccgcc tcctccgccc gcgcaacgaa gacatccggc gggacgaaat cgcccttgcg gatttcccat cggatcaatg cttcaaagcc gagcaccttg ccatccgcca tatccaccac cggctggtag agcaaatgcg gctctccggc agcgagtgcc ttgttcagcc tgtagagcaa tgagtggtcg cgattgcgca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcaatcgc gaccactcat tgctctacag gctgaacaag gcactcgctg ccggagagcc gcatttgctc taccagccgg tggtggatat ggcggatggc aaggtgctcg gctttgaagc attgatccga tgggaaatcc gcaagggcga tttcgtcccg ccggatgtct tcgttgcgcg ggcggaggag gcgggcaccg ctacaaagat aacgatctat gtgctggaac gtctgataga agacatgggc gacacgctgc gccagcgccg tgatttgcgt atcaatatca acattaccgc cagcgacctg caaagcccgg ccttcatgag ccggatggaa aggcgccttg cggcggctga tatcgaacca caacaggtgg ggttggaact gacggaacgc acggcggtcg atttttccaa ggcgacggac ggcatcaggc gcctgcgcga gcaggggcac cggatttata tcgacgattt cggcaccggc tattcgagcc ttgcctatct cggcgaattg catgtggacg caatcaagat cgacaaggct ttcacgcgca ctgtcggcaa tgattacgca acggtctcca tcattccgca gatcatttcc atggcgaagg aacatggcct cgatatcgtg gtcgaagggg tggaaacgga ggcacaggtc ctctattttc gtaaactggg cacgcccctg gccgcacaag gctggttttt tggcaagccg ctgtgcgttt ccgatgcgca ggcgctggtc gctacagtga agaagaagca atcgcgcgtg aagttggccc gcaaaacgac ccaaaaggcg aaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BrmeA.18138.a.A3.PS01269

PURIFICATION DATe:	11/23/2011
CONCENTRATION:	16.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCAATCGC GACCACTCAT TGCTCTACAG GCTGAACAAG GCACTCGCTG CCGGAGAGCC GCATTTGCTC TACCAGCCGG TGGTGGATAT GGCGGATGGC AAGGTGCTCG GCTTTGAAGC ATTGATCCGA TGGGAAATCC GCAAGGGCGA TTTCGTCCCG CCGGATGTCT TCGTTGCGCG GGCGGAGGAG GCGGGCACCG CTACAAAGAT AACGATCTAT GTGCTGGAAC GTCTGATAGA AGACATGGGC GACACGCTGC GCCAGCGCCG TGATTTGCGT ATCAATATCA ACATTACCGC CAGCGACCTG CAAAGCCCGG CCTTCATGAG CCGGATGGAA AGGCGCCTTG CGGCGGCTGA TATCGAACCA CAACAGGTGG GGTTGGAACT GACGGAACGC ACGGCGGTCG ATTTTTCCAA GGCGACGGAC GGCATCAGGC GCCTGCGCGA GCAGGGGCAC CGGATTTATA TCGACGATTT CGGCACCGGC TATTCGAGCC TTGCCTATCT CGGCGAATTG CATGTGGACG CAATCAAGAT CGACAAGGCT TTCACGCGCA CTGTCGGCAA TGATTACGCA ACGGTCTCCA TCATTCCGCA GATCATTTCC ATGGCGAAGG AACATGGCCT CGATATCGTG GTCGAAGGGG TGGAAACGGA GGCACAGGTC CTCTATTTTC GTAAACTGGG CACGCCCCTG GCCGCACAAG GCTGGTTTTT TGGCAAGCCG CTGTGCGTTT CCGATGCGCA GGCGCTGGTC GCTACAGTGA AGAAGAAGCA ATCGCGCGTG AAGTTGGCCC GCAAAACGAC CCAAAAGGCG AAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA