BrmeA.18135.a
D-erythrulose 4-phosphate dehydrogenase (EC 1.1.1.-)
| CENTER ID: | BrmeA.18135.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18135.a.A1.GE33787 | full length | 1 | 310 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18135.a.A1.PS01411 | full length | 1 | 310 |
Structures
4K3Z
DEPOSITED: 4/11/2013
DETERMINATION: XRay
CLONE: BrmeA.18135.a.A1.GE33787
PROTEIN: BrmeA.18135.a.A1.PS01411
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | NO_GROUP |
| PATRIC ID: | fig|224914.11.peg.2735 |
| RefSeq: | NP_541406.1 |
| UniProt: | Q8YCV0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALTLSLNTN PLVNRFAEPD DLIETVARDL RLRDLQLTHE FINPSWQAST IRRLTRDMDR ALQRTGVRVT SGMTGPYGRL NHFGHPDRDV RRYYVDWFKT FADIIGDLGG KSVGTQFAIF TYKDFDDPAR REELIKIAID CWAEVAEHAA GAGLDYVFWE PMSIGREFGE TIAECMKLQD RLTAANMAIP MWMMADIDHG DVTSANPDDY DPYAWARTVP KVSPIIHIKQ SLMDKGGHRP FTAAFNAKGR IQPEPLLKAF AEGGAVDNEI CLELSFKERE PNDREVIPQI AESVAFWAPH IDTGAKDLKI
NT Sequence
atggccctga cgctttcgct caacaccaat ccgctggtca accgctttgc cgagccggac gacctgatcg aaacggttgc ccgcgacctg cgcctgcgtg acctccagct tacccatgag ttcatcaatc caagctggca ggcctcgacc atccgccgcc tcacccgcga catggacagg gccttgcagc gcaccggcgt ccgcgtcacc tccggcatga ccggccccta tggccgcctc aaccattttg gccatcccga ccgggacgtg cgccgctatt atgtggactg gttcaagacc tttgccgata ttatcggcga tcttggcggc aagtccgtcg gtacgcagtt tgcaatcttc acctataagg atttcgatga tccggcgcgc cgcgaagaac ttatcaagat cgccatcgac tgctgggccg aggtggccga acatgcggca ggtgcgggcc tcgactatgt gttctgggag ccgatgagca tcgggcgcga atttggcgag acgattgccg aatgcatgaa gcttcaggat cggctcaccg ccgctaacat ggcgatcccc atgtggatga tggccgatat cgaccatggt gacgtgacat ccgctaaccc ggacgactac gatccttacg catgggcccg caccgtgccg aaagtctcgc ccatcatcca tatcaagcaa agcctgatgg acaagggcgg gcatcgtcct ttcacagccg cgttcaatgc caagggccgc atccagccgg aaccgctttt gaaagccttt gccgaaggcg gcgcggtgga taatgaaatc tgccttgaac tttcgttcaa ggagcgcgag ccgaacgacc gtgaagtcat tccacagatt gcagaaagtg tggctttctg ggcgccgcac attgacaccg gcgctaagga cttgaagata tag
Details for BrmeA.18135.a.A1.GE33787
| HARVESTED ON: | 10/4/2011 |
| SEQUENCED ON: | 10/7/2011 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MALTLSLNTN PLVNRFAEPD DLIETVARDL RLRDLQLTHE FINPSWQAST IRRLTRDMDR ALQRTGVRVT SGMTGPYGRL NHFGHPDRDV RRYYVDWFKT FADIIGDLGG KSVGTQFAIF TYKDFDDPAR REELIKIAID CWAEVAEHAA GAGLDYVFWE PMSIGREFGE TIAECMKLQD RLTAANMAIP MWMMADIDHG DVTSANPDDY DPYAWARTVP KVSPIIHIKQ SLMDKGGHRP FTAAFNAKGR IQPEPLLKAF AEGGAVDNEI CLELSFKERE PNDREVIPQI AESVAFWAPH IDTGAKDL
Validated NT Sequence
tcaagtcctt agcgccggtg tcaatgtgcg gcgcccagaa agccacactt tctgcaatct gtggaatgac ttcacggtcg ttcggctcgc gctccttgaa cgaaagttca aggcagattt cattatccac cgcgccgcct tcggcaaagg ctttcaaaag cggttccggc tggatgcggc ccttggcatt gaacgcggct gtgaaaggac gatgcccgcc cttgtccatc aggctttgct tgatatggat gatgggcgag actttcggca cggtgcgggc ccatgcgtaa ggatcgtagt cgtccgggtt agcggatgtc acgtcaccat ggtcgatatc ggccatcatc cacatgggga tcgccatgtt agcggcggtg agccgatcct gaagcttcat gcattcggca atcgtctcgc caaattcgcg cccgatgctc atcggctccc agaacacata gtcgaggccc gcacctgccg catgttcggc cacctcggcc cagcagtcga tggcgatctt gataagttct tcgcggcgcg ccggatcatc gaaatcctta taggtgaaga ttgcaaactg cgtaccgacg gacttgccgc caagatcgcc gataatatcg gcaaaggtct tgaaccagtc cacataatag cggcgcacgt cccggtcggg atggccaaaa tggttgaggc ggccataggg gccggtcatg ccggaggtga cgcggacgcc ggtgcgctgc aaggccctgt ccatgtcgcg ggtgaggcgg cggatggtcg aggcctgcca gcttggattg atgaactcat gggtaagctg gaggtcacgc aggcgcaggt cgcgggcaac cgtttcgatc aggtcgtccg gctcggcaaa gcggttgacc agcggattgg tgttgagcga aagcgtcagg gccatcgaac caggaccctg ggtctgagct tccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALTLSLNT NPLVNRFAEP DDLIETVARD LRLRDLQLTH EFINPSWQAS TIRRLTRDMD RALQRTGVRV TSGMTGPYGR LNHFGHPDRD VRRYYVDWFK TFADIIGDLG GKSVGTQFAI FTYKDFDDPA RREELIKIAI DCWAEVAEHA AGAGLDYVFW EPMSIGREFG ETIAECMKLQ DRLTAANMAI PMWMMADIDH GDVTSANPDD YDPYAWARTV PKVSPIIHIK QSLMDKGGHR PFTAAFNAKG RIQPEPLLKA FAEGGAVDNE ICLELSFKER EPNDREVIPQ IAESVAFWAP HIDTGAKDLK I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccctgacg ctttcgctca acaccaatcc gctggtcaac cgctttgccg agccggacga cctgatcgaa acggttgccc gcgacctgcg cctgcgtgac ctccagctta cccatgagtt catcaatcca agctggcagg cctcgaccat ccgccgcctc acccgcgaca tggacagggc cttgcagcgc accggcgtcc gcgtcacctc cggcatgacc ggcccctatg gccgcctcaa ccattttggc catcccgacc gggacgtgcg ccgctattat gtggactggt tcaagacctt tgccgatatt atcggcgatc ttggcggcaa gtccgtcggt acgcagtttg caatcttcac ctataaggat ttcgatgatc cggcgcgccg cgaagaactt atcaagatcg ccatcgactg ctgggccgag gtggccgaac atgcggcagg tgcgggcctc gactatgtgt tctgggagcc gatgagcatc gggcgcgaat ttggcgagac gattgccgaa tgcatgaagc ttcaggatcg gctcaccgcc gctaacatgg cgatccccat gtggatgatg gccgatatcg accatggtga cgtgacatcc gctaacccgg acgactacga tccttacgca tgggcccgca ccgtgccgaa agtctcgccc atcatccata tcaagcaaag cctgatggac aagggcgggc atcgtccttt cacagccgcg ttcaatgcca agggccgcat ccagccggaa ccgcttttga aagcctttgc cgaaggcggc gcggtggata atgaaatctg ccttgaactt tcgttcaagg agcgcgagcc gaacgaccgt gaagtcattc cacagattgc agaaagtgtg gctttctggg cgccgcacat tgacaccggc gctaaggact tgaagatata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrmeA.18135.a.A1.PS01411
| PURIFICATION DATe: | 3/8/2012 |
| CONCENTRATION: | 53.68mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MALTLSLNTN PLVNRFAEPD DLIETVARDL RLRDLQLTHE FINPSWQAST IRRLTRDMDR ALQRTGVRVT SGMTGPYGRL NHFGHPDRDV RRYYVDWFKT FADIIGDLGG KSVGTQFAIF TYKDFDDPAR REELIKIAID CWAEVAEHAA GAGLDYVFWE PMSIGREFGE TIAECMKLQD RLTAANMAIP MWMMADIDHG DVTSANPDDY DPYAWARTVP KVSPIIHIKQ SLMDKGGHRP FTAAFNAKGR IQPEPLLKAF AEGGAVDNEI CLELSFKERE PNDREVIPQI AESVAFWAPH IDTGAKDL
Validated NT Sequence
tcaagtcctt agcgccggtg tcaatgtgcg gcgcccagaa agccacactt tctgcaatct gtggaatgac ttcacggtcg ttcggctcgc gctccttgaa cgaaagttca aggcagattt cattatccac cgcgccgcct tcggcaaagg ctttcaaaag cggttccggc tggatgcggc ccttggcatt gaacgcggct gtgaaaggac gatgcccgcc cttgtccatc aggctttgct tgatatggat gatgggcgag actttcggca cggtgcgggc ccatgcgtaa ggatcgtagt cgtccgggtt agcggatgtc acgtcaccat ggtcgatatc ggccatcatc cacatgggga tcgccatgtt agcggcggtg agccgatcct gaagcttcat gcattcggca atcgtctcgc caaattcgcg cccgatgctc atcggctccc agaacacata gtcgaggccc gcacctgccg catgttcggc cacctcggcc cagcagtcga tggcgatctt gataagttct tcgcggcgcg ccggatcatc gaaatcctta taggtgaaga ttgcaaactg cgtaccgacg gacttgccgc caagatcgcc gataatatcg gcaaaggtct tgaaccagtc cacataatag cggcgcacgt cccggtcggg atggccaaaa tggttgaggc ggccataggg gccggtcatg ccggaggtga cgcggacgcc ggtgcgctgc aaggccctgt ccatgtcgcg ggtgaggcgg cggatggtcg aggcctgcca gcttggattg atgaactcat gggtaagctg gaggtcacgc aggcgcaggt cgcgggcaac cgtttcgatc aggtcgtccg gctcggcaaa gcggttgacc agcggattgg tgttgagcga aagcgtcagg gccatcgaac caggaccctg ggtctgagct tccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALTLSLNT NPLVNRFAEP DDLIETVARD LRLRDLQLTH EFINPSWQAS TIRRLTRDMD RALQRTGVRV TSGMTGPYGR LNHFGHPDRD VRRYYVDWFK TFADIIGDLG GKSVGTQFAI FTYKDFDDPA RREELIKIAI DCWAEVAEHA AGAGLDYVFW EPMSIGREFG ETIAECMKLQ DRLTAANMAI PMWMMADIDH GDVTSANPDD YDPYAWARTV PKVSPIIHIK QSLMDKGGHR PFTAAFNAKG RIQPEPLLKA FAEGGAVDNE ICLELSFKER EPNDREVIPQ IAESVAFWAP HIDTGAKDLK I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCCTGACG CTTTCGCTCA ACACCAATCC GCTGGTCAAC CGCTTTGCCG AGCCGGACGA CCTGATCGAA ACGGTTGCCC GCGACCTGCG CCTGCGTGAC CTCCAGCTTA CCCATGAGTT CATCAATCCA AGCTGGCAGG CCTCGACCAT CCGCCGCCTC ACCCGCGACA TGGACAGGGC CTTGCAGCGC ACCGGCGTCC GCGTCACCTC CGGCATGACC GGCCCCTATG GCCGCCTCAA CCATTTTGGC CATCCCGACC GGGACGTGCG CCGCTATTAT GTGGACTGGT TCAAGACCTT TGCCGATATT ATCGGCGATC TTGGCGGCAA GTCCGTCGGT ACGCAGTTTG CAATCTTCAC CTATAAGGAT TTCGATGATC CGGCGCGCCG CGAAGAACTT ATCAAGATCG CCATCGACTG CTGGGCCGAG GTGGCCGAAC ATGCGGCAGG TGCGGGCCTC GACTATGTGT TCTGGGAGCC GATGAGCATC GGGCGCGAAT TTGGCGAGAC GATTGCCGAA TGCATGAAGC TTCAGGATCG GCTCACCGCC GCTAACATGG CGATCCCCAT GTGGATGATG GCCGATATCG ACCATGGTGA CGTGACATCC GCTAACCCGG ACGACTACGA TCCTTACGCA TGGGCCCGCA CCGTGCCGAA AGTCTCGCCC ATCATCCATA TCAAGCAAAG CCTGATGGAC AAGGGCGGGC ATCGTCCTTT CACAGCCGCG TTCAATGCCA AGGGCCGCAT CCAGCCGGAA CCGCTTTTGA AAGCCTTTGC CGAAGGCGGC GCGGTGGATA ATGAAATCTG CCTTGAACTT TCGTTCAAGG AGCGCGAGCC GAACGACCGT GAAGTCATTC CACAGATTGC AGAAAGTGTG GCTTTCTGGG CGCCGCACAT TGACACCGGC GCTAAGGACT TGAAGATATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A