BrmeA.18134.b

Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)

CENTER ID: BrmeA.18134.b
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18134.b.B1.GE36712 Full length( BrmeA.18134.b ) 1 500
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_1936
BV-BRC: fig|224914.11.peg.1936
RefSeq: NP_540666.1
RefSeq: WP_011005390.1
UniProt: Q8YEX8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVQEKQFDIF IIGGGINGCG IARDAVGRGF TVGLAEMNDL ASGTSSRATK LIHGGLRYLE HYEFRLVREA LMEREVLWAN APHIIHPMRF VLPYHKGGLR PAWLLRLGLF LYDHLGGRKK LPATRTLNMR TDKAGEPLKP LFTKAFEYSD CWVDDARFVA LTARDAADRG AKIATRTSVV AASRDGQGWT ITLEDTGTGR RENVHARLLV NAAGPWADKV LQSVEGDRQL HNIRLVQGSH IVVRRKFSDP RAYFFQNNDG RIIFAIPYED DFTLIGTTDQ DYKGDPAKVA ITDSETDYLC QAASEYFREP VRREDIVWTY SGVRPLYDDG ASKAQEATRD YVLKEDAPDG LAPLINVFGG KLTTARKLAE HMLQKIEHWL GRKGAPWTHA APLPGGDFED VAFETELKKL EAAYPFLDAR RLFRLYGTRA YKLLGQASSL GDLGRHFGSD LYEAEVRYLV ENEWARSAED ILWRRTKLGL RLTAAEVAAV QGFVEPAIAA
NT Sequence
ATGGTGCAGG AAAAACAATT CGATATTTTT ATTATAGGTG GCGGCATCAA TGGCTGCGGC ATTGCCCGTG ATGCGGTGGG GCGGGGCTTC ACCGTTGGGC TGGCCGAGAT GAACGATCTG GCAAGCGGCA CCTCTTCGCG CGCAACCAAG CTCATTCACG GTGGTTTGCG CTATCTGGAA CATTATGAAT TCCGGCTGGT GCGCGAGGCG CTGATGGAGC GCGAGGTGCT GTGGGCCAAT GCGCCGCATA TCATCCATCC GATGCGTTTC GTGCTGCCTT ATCATAAGGG CGGCCTACGT CCCGCCTGGC TGTTGCGGCT TGGCCTGTTT CTTTACGACC ATCTGGGCGG CCGCAAGAAG CTTCCCGCCA CGCGCACGCT CAACATGCGC ACCGACAAGG CCGGCGAACC GCTGAAACCG CTTTTCACCA AGGCTTTTGA ATATTCCGAT TGCTGGGTGG ACGATGCGCG CTTCGTGGCG CTCACCGCCC GCGATGCGGC TGATCGCGGC GCGAAGATCG CGACCCGAAC GAGCGTCGTT GCCGCAAGCC GCGACGGGCA GGGCTGGACC ATCACACTGG AAGATACCGG CACCGGCAGG CGTGAGAACG TACACGCCCG CCTTCTGGTC AATGCCGCCG GGCCGTGGGC CGACAAGGTT CTGCAAAGTG TGGAAGGCGA CCGGCAATTG CACAATATCC GTCTGGTGCA GGGCAGCCAT ATCGTCGTGC GCAGGAAGTT TTCCGACCCG CGCGCCTATT TCTTCCAGAA CAACGACGGG CGCATCATCT TCGCCATTCC TTATGAAGAT GATTTCACCC TGATTGGCAC CACGGATCAG GATTATAAGG GCGACCCGGC CAAGGTGGCG ATCACCGACA GCGAGACCGA TTATCTCTGC CAGGCGGCAA GCGAATATTT CCGCGAGCCG GTGCGCCGGG AGGATATCGT CTGGACCTAT TCCGGCGTGC GCCCGCTCTA TGATGACGGG GCCAGCAAGG CGCAGGAGGC AACCCGCGAC TATGTGCTGA AGGAAGATGC GCCAGATGGT CTGGCGCCGC TCATCAATGT TTTCGGCGGC AAGCTCACCA CAGCACGCAA GCTTGCCGAA CATATGCTGC AGAAAATCGA GCACTGGCTT GGCCGCAAGG GCGCCCCATG GACCCATGCA GCACCATTGC CAGGCGGGGA TTTCGAGGAT GTGGCTTTTG AAACGGAACT GAAGAAGCTT GAGGCAGCCT ATCCTTTCCT TGATGCGCGC CGCCTGTTCC GGCTCTATGG CACCCGGGCA TATAAGCTTT TGGGGCAGGC TTCTTCGCTG GGCGATCTTG GCCGTCATTT TGGCAGCGAT CTCTATGAGG CGGAAGTGCG CTATCTTGTC GAAAATGAAT GGGCGCGAAG CGCGGAAGAC ATTTTGTGGC GGCGCACCAA GCTCGGCCTG CGCCTGACGG CGGCGGAAGT CGCGGCCGTG CAGGGCTTTG TGGAGCCTGC AATCGCCGCC
Details for BrmeA.18134.b.B1.GE36712
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 91
Validated AA Sequence
MNDLASGTSS RATKLIHGGL RYLEHYEFRL VREALMEREV LWANAPHIIH PMRFVLPYHK GGLRPAWLLR LGLFLYDHLG GRKKLPATRT LNMRTDKAGE PLKPLFTKAF EYSDCWVDDA RFVALTARDA ADRGAKIATR TSVVAASRDG QGWTITLEDT GTGRRENVHA RLLVNAAGPX ADKVLQSVEG DRQLHNIRLV QGSHIVVRRK FSDPRAYFFQ NNDGRIIFAI PYEDDFTLIG TTDQDYKGDP AKVAIXDSET DYLCQAAXEY XREPVRREDX VWTYSGVRPL YDXGASKAQE ATXXYVXKED APDGLAPLIN VFGGKLTTAR KLAEHMLQKI EHWLGRKGAP WTHAAPLPGG DXEDVAFETE LKKLEAAYPF LDARRLFRLY GTRAYKLLGQ ASSLGDLGRH FGSDLYEAEV RYLVENEWAR SAEDILWRRT KLGLRLTAAE VAAVQGFVEP AIAA
Validated NT Sequence
atcttactca cttaggcggc gattgcaggc tccacaaagc cctgcacggc cgcgacttcc gccgccgtca ggcgcaggcc gagcttggtg cgccgccaca aaatgtcttc cgcgcttcgc gcccattcat tttcgacaag atagcgcact tccgcctcat agagatcgct gccaaaatga cggccaagat cgcccagcga agaagcctgc cccaaaagct tatatgcccg ggtgccatan agccggaaca ggcggcgcgc atcaaggaaa ggataggctg cctcaagctt cttcagttcc gtttcaaaag ccacatcctc naaatccccg cctggcaatg gtgctgcatg ggtccatggg gcgcccttgc ggccaagcca gtgctcgatt ttctgcagca tatgttcggc aagcttgcgt gctgtggtga gcttgccgcc gaaaacattg atgagcggcg ccagaccatc tggcgcatct tccttcnnca catagncnng ggttgcctcc tgcgccttgc tggccccgnc atcatagagc gggcgcacgc cngaatangt ccagacnata tcctcccggc gcaccggctc gcgnaaatat tcncttgccg cctggcagag ataatcggtc tcgctgtcgg ngatcgccac cttggccggg tcgcccttat aatcctgatc cgtggtgcca atcagggtga aatcatcttc ataaggaatg gcgaagatga tgcgcccgtc gttgttctgg aagaaatagg cgcgcgggtc ggaaaacttc ctgcgcacga cgatatggct gccctgcacc agacggatat tgtgcaattg ccggtcgcct tccacacttt gcagaacctt gtcggcncac ggcccggcgg cattgaccag aaggcgggcg tgtacgttct cacgcctgcc ggtgccggta tcttccagtg tgatggtcca gccctgcccg tcgcggcttg cggcaacgac gctcgttcgg gtcgcgatct tcgcgccgcg atcagccgca tcgcgggcgg tgagcgccac gaagcgcgca tcgtccaccc agcaatcgga atattcaaaa gccttggtga aaagcggttt cagcggttcg ccggccttgt cggtgcgcat gttgagcgtg cgcgtggcgg gaagcttctt gcggccgccc agatggtcgt aaagaaacag gccaagccgc aacagccagg cgggacgtag gccgccctta tgataaggca gcacgaaacg catcggatgg atgatatgcg gcgcattggc ccacagcacc tcgcgctcca tcagcgcctc gcgcaccagc cggaattcat aatgttccag atagcgcaaa ccaccgtgaa tgagcttggt tgcgcgcgaa gaggtgccgc ttgccagatc gttcatctcg gccagcccaa cggtgaagcc ccgccccacc gcatcacggg caatgccgca gccattgatg ccgccaccta taataaaaat atcgaattgt ttttcct
Expected Protein Sequence
MAHHHHHHMV QEKQFDIFII GGGINGCGIA RDAVGRGFTV GLAEMNDLAS GTSSRATKLI HGGLRYLEHY EFRLVREALM EREVLWANAP HIIHPMRFVL PYHKGGLRPA WLLRLGLFLY DHLGGRKKLP ATRTLNMRTD KAGEPLKPLF TKAFEYSDCW VDDARFVALT ARDAADRGAK IATRTSVVAA SRDGQGWTIT LEDTGTGRRE NVHARLLVNA AGPWADKVLQ SVEGDRQLHN IRLVQGSHIV VRRKFSDPRA YFFQNNDGRI IFAIPYEDDF TLIGTTDQDY KGDPAKVAIT DSETDYLCQA ASEYFREPVR REDIVWTYSG VRPLYDDGAS KAQEATRDYV LKEDAPDGLA PLINVFGGKL TTARKLAEHM LQKIEHWLGR KGAPWTHAAP LPGGDFEDVA FETELKKLEA AYPFLDARRL FRLYGTRAYK LLGQASSLGD LGRHFGSDLY EAEVRYLVEN EWARSAEDIL WRRTKLGLRL TAAEVAAVQG FVEPAIAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc aggaaaaaca attcgatatt tttattatag gtggcggcat caatggctgc ggcattgccc gtgatgcggt ggggcggggc ttcaccgttg ggctggccga gatgaacgat ctggcaagcg gcacctcttc gcgcgcaacc aagctcattc acggtggttt gcgctatctg gaacattatg aattccggct ggtgcgcgag gcgctgatgg agcgcgaggt gctgtgggcc aatgcgccgc atatcatcca tccgatgcgt ttcgtgctgc cttatcataa gggcggccta cgtcccgcct ggctgttgcg gcttggcctg tttctttacg accatctggg cggccgcaag aagcttcccg ccacgcgcac gctcaacatg cgcaccgaca aggccggcga accgctgaaa ccgcttttca ccaaggcttt tgaatattcc gattgctggg tggacgatgc gcgcttcgtg gcgctcaccg cccgcgatgc ggctgatcgc ggcgcgaaga tcgcgacccg aacgagcgtc gttgccgcaa gccgcgacgg gcagggctgg accatcacac tggaagatac cggcaccggc aggcgtgaga acgtacacgc ccgccttctg gtcaatgccg ccgggccgtg ggccgacaag gttctgcaaa gtgtggaagg cgaccggcaa ttgcacaata tccgtctggt gcagggcagc catatcgtcg tgcgcaggaa gttttccgac ccgcgcgcct atttcttcca gaacaacgac gggcgcatca tcttcgccat tccttatgaa gatgatttca ccctgattgg caccacggat caggattata agggcgaccc ggccaaggtg gcgatcaccg acagcgagac cgattatctc tgccaggcgg caagcgaata tttccgcgag ccggtgcgcc gggaggatat cgtctggacc tattccggcg tgcgcccgct ctatgatgac ggggccagca aggcgcagga ggcaacccgc gactatgtgc tgaaggaaga tgcgccagat ggtctggcgc cgctcatcaa tgttttcggc ggcaagctca ccacagcacg caagcttgcc gaacatatgc tgcagaaaat cgagcactgg cttggccgca agggcgcccc atggacccat gcagcaccat tgccaggcgg ggatttcgag gatgtggctt ttgaaacgga actgaagaag cttgaggcag cctatccttt ccttgatgcg cgccgcctgt tccggctcta tggcacccgg gcatataagc ttttggggca ggcttcttcg ctgggcgatc ttggccgtca ttttggcagc gatctctatg aggcggaagt gcgctatctt gtcgaaaatg aatgggcgcg aagcgcggaa gacattttgt ggcggcgcac caagctcggc ctgcgcctga cggcggcgga agtcgcggcc gtgcagggct ttgtggagcc tgcaatcgcc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat