BrmeA.18116.b
hypothetical protein
| CENTER ID: | BrmeA.18116.b |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18116.b.B2.GE36711 | Mature protein( BrmeA.18116.b ) | 15 | 215 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.18116.b.B2.PS01846 | Mature protein( BrmeA.18116.b ) | 15 | 215 |
Structures
6V45
DEPOSITED: 11/27/2019
DETERMINATION: XRay
CLONE: BrmeA.18116.b.B2.GE36711
PROTEIN: BrmeA.18116.b.B2.PS01846
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|224914.11.peg.955 |
| RefSeq: | NP_539765.1 |
| RefSeq: | WP_011005171.1 |
| UniProt: | Q8YHF3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGAAAPAFVA SPVNAEAQLD EAAANVQKVA LASNAKANPD KPKKKTAART NNTAKANKYS IDPKFRPQDV TFTGYKPGTI VIDPKKRFLY LVETSTTARR YGIAVGKQGL EFQGKATISA KREWPRWIPT KEMIERDPAH YGRFKNGMDG GPGNPLGSRA MYLFQGNKDT YIRIHGTVQP WTIGSSASNG CFRMINEDVM DLYDRVTLGT EVVVL
NT Sequence
ATGGGAGCCG CTGCTCCTGC CTTTGTTGCT TCGCCGGTCA ATGCTGAAGC GCAGCTCGAT GAAGCTGCGG CAAATGTCCA GAAGGTCGCT CTGGCGAGCA ACGCCAAGGC AAACCCGGAC AAGCCGAAGA AGAAAACGGC TGCCAGGACC AACAATACGG CCAAGGCCAA CAAATATAGC ATCGATCCGA AGTTTCGTCC GCAGGACGTG ACCTTCACCG GCTACAAGCC CGGCACAATC GTGATCGATC CCAAGAAGCG CTTCCTCTAT CTGGTGGAAA CTTCCACGAC CGCGCGCCGC TATGGCATTG CCGTTGGCAA GCAGGGACTG GAATTTCAGG GCAAGGCCAC CATCAGCGCC AAGCGCGAAT GGCCGCGCTG GATTCCCACC AAGGAAATGA TTGAGCGCGA TCCGGCCCAT TATGGCCGCT TCAAGAACGG CATGGATGGT GGCCCGGGCA ATCCGCTCGG CTCGCGTGCG ATGTATCTCT TCCAGGGCAA CAAAGACACC TATATCCGCA TCCACGGTAC GGTGCAGCCA TGGACCATCG GCAGCTCTGC CTCCAATGGC TGCTTCCGCA TGATCAACGA AGACGTGATG GATCTCTACG ACCGCGTTAC GCTTGGTACG GAAGTGGTTG TTCTG
Details for BrmeA.18116.b.B2.GE36711
| HARVESTED ON: | 2/27/2013 |
| SEQUENCED ON: | 2/2/2013 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA EAQLDEAAAN VQKVALASNA KANPDKPKKK TAARTNNTAK ANKYSIDPKF RPQDVTFTGY KPGTIVIDPK KRFLYLVETS TTARRYGIAV GKQGLEFQGK ATISAKREWP RWIPTKEMIE RDPAHYGRFK NGMDGGPGNP LGSRAMYLFQ GNKDTYIRIH GTVQPWTIGS SASNGCFRMI NEDVMDLYDR VTLGTEVVVL
Validated NT Sequence
atcttactca cttacagaac aaccacttcc gtaccaagcg taacgcggtc gtagagatcc atcacgtctt cgttgatcat gcggaagcag ccattggagg cagagctgcc gatggtccat ggctgcaccg taccgtggat gcggatatag gtgtctttgt tgccctggaa gagatacatc gcacgcgagc cgagcggatt gcccgggcca ccatccatgc cgttcttgaa gcggccataa tgggccggat cgcgctcaat catttccttg gtgggaatcc agcgcggcca ttcgcgcttg gcgctgatgg tggccttgcc ctgaaattcc agtccctgct tgccaacggc aatgccatag cggcgcgcgg tcgtggaagt ttccaccaga tagaggaagc gcttcttggg atcgatcacg attgtgccgg gcttgtagcc ggtgaaggtc acgtcctgcg gacgaaactt cggatcgatg ctatatttgt tggccttggc cgtattgttg gtcctggcag ccgttttctt cttcggcttg tccgggtttg ccttggcgtt gctcgccaga gcgaccttct ggacatttgc cgcagcttca tcgagctgcg cttcagccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAE AQLDEAAANV QKVALASNAK ANPDKPKKKT AARTNNTAKA NKYSIDPKFR PQDVTFTGYK PGTIVIDPKK RFLYLVETST TARRYGIAVG KQGLEFQGKA TISAKREWPR WIPTKEMIER DPAHYGRFKN GMDGGPGNPL GSRAMYLFQG NKDTYIRIHG TVQPWTIGSS ASNGCFRMIN EDVMDLYDRV TLGTEVVVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgaag cgcagctcga tgaagctgcg gcaaatgtcc agaaggtcgc tctggcgagc aacgccaagg caaacccgga caagccgaag aagaaaacgg ctgccaggac caacaatacg gccaaggcca acaaatatag catcgatccg aagtttcgtc cgcaggacgt gaccttcacc ggctacaagc ccggcacaat cgtgatcgat cccaagaagc gcttcctcta tctggtggaa acttccacga ccgcgcgccg ctatggcatt gccgttggca agcagggact ggaatttcag ggcaaggcca ccatcagcgc caagcgcgaa tggccgcgct ggattcccac caaggaaatg attgagcgcg atccggccca ttatggccgc ttcaagaacg gcatggatgg tggcccgggc aatccgctcg gctcgcgtgc gatgtatctc ttccagggca acaaagacac ctatatccgc atccacggta cggtgcagcc atggaccatc ggcagctctg cctccaatgg ctgcttccgc atgatcaacg aagacgtgat ggatctctac gaccgcgtta cgcttggtac ggaagtggtt gttctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrmeA.18116.b.B2.PS01846
| PURIFICATION DATe: | 6/19/2013 |
| CONCENTRATION: | 18.1mg/ml |
| OBSERVED MW: | 24kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA EAQLDEAAAN VQKVALASNA KANPDKPKKK TAARTNNTAK ANKYSIDPKF RPQDVTFTGY KPGTIVIDPK KRFLYLVETS TTARRYGIAV GKQGLEFQGK ATISAKREWP RWIPTKEMIE RDPAHYGRFK NGMDGGPGNP LGSRAMYLFQ GNKDTYIRIH GTVQPWTIGS SASNGCFRMI NEDVMDLYDR VTLGTEVVVL
Validated NT Sequence
atcttactca cttacagaac aaccacttcc gtaccaagcg taacgcggtc gtagagatcc atcacgtctt cgttgatcat gcggaagcag ccattggagg cagagctgcc gatggtccat ggctgcaccg taccgtggat gcggatatag gtgtctttgt tgccctggaa gagatacatc gcacgcgagc cgagcggatt gcccgggcca ccatccatgc cgttcttgaa gcggccataa tgggccggat cgcgctcaat catttccttg gtgggaatcc agcgcggcca ttcgcgcttg gcgctgatgg tggccttgcc ctgaaattcc agtccctgct tgccaacggc aatgccatag cggcgcgcgg tcgtggaagt ttccaccaga tagaggaagc gcttcttggg atcgatcacg attgtgccgg gcttgtagcc ggtgaaggtc acgtcctgcg gacgaaactt cggatcgatg ctatatttgt tggccttggc cgtattgttg gtcctggcag ccgttttctt cttcggcttg tccgggtttg ccttggcgtt gctcgccaga gcgaccttct ggacatttgc cgcagcttca tcgagctgcg cttcagccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHAE AQLDEAAANV QKVALASNAK ANPDKPKKKT AARTNNTAKA NKYSIDPKFR PQDVTFTGYK PGTIVIDPKK RFLYLVETST TARRYGIAVG KQGLEFQGKA TISAKREWPR WIPTKEMIER DPAHYGRFKN GMDGGPGNPL GSRAMYLFQG NKDTYIRIHG TVQPWTIGSS ASNGCFRMIN EDVMDLYDRV TLGTEVVVL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGAAG CGCAGCTCGA TGAAGCTGCG GCAAATGTCC AGAAGGTCGC TCTGGCGAGC AACGCCAAGG CAAACCCGGA CAAGCCGAAG AAGAAAACGG CTGCCAGGAC CAACAATACG GCCAAGGCCA ACAAATATAG CATCGATCCG AAGTTTCGTC CGCAGGACGT GACCTTCACC GGCTACAAGC CCGGCACAAT CGTGATCGAT CCCAAGAAGC GCTTCCTCTA TCTGGTGGAA ACTTCCACGA CCGCGCGCCG CTATGGCATT GCCGTTGGCA AGCAGGGACT GGAATTTCAG GGCAAGGCCA CCATCAGCGC CAAGCGCGAA TGGCCGCGCT GGATTCCCAC CAAGGAAATG ATTGAGCGCG ATCCGGCCCA TTATGGCCGC TTCAAGAACG GCATGGATGG TGGCCCGGGC AATCCGCTCG GCTCGCGTGC GATGTATCTC TTCCAGGGCA ACAAAGACAC CTATATCCGC ATCCACGGTA CGGTGCAGCC ATGGACCATC GGCAGCTCTG CCTCCAATGG CTGCTTCCGC ATGATCAACG AAGACGTGAT GGATCTCTAC GACCGCGTTA CGCTTGGTAC GGAAGTGGTT GTTCTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T