SSGCID: Target Page

BrmeA.18116.a

Protein ybis

CENTER ID:	BrmeA.18116.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18116.a.A1.GE33704	full length		1	206

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.2000
RefSeq:	NP_540726.1
UniProt:	Q8YER8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSRVLKLTA ALFLGAALAG CSTAGGSFYT ASYSSVSDAG YAIPAIPSEK IPAQYRRQVV KYATDEKPGT IIVDTREKFL YLIMPEGKAV RYGIGVGRRG FEWSGTARVA MKREWPTWTP PSAMIKRQPE LAKYRNGMDP GLRNPLGARA LYLYNKGGDT GYRLHGTPEW WSIGKAMSSG CIRLMNQDII DLYNRVEQGA KVVVKQ

NT Sequence

atggattctc gcgttctcaa gctgacagcg gctctttttc ttggagcagc acttgctggt tgctccacag ccggtggttc tttctacacg gcgtcttact cgtcagtcag cgatgctggt tacgccatcc cggcaatccc gagcgaaaag ataccggcgc aatatcgccg tcaggttgta aaatatgcga cggacgaaaa gccgggcacc atcattgtcg atacgcgtga gaaatttctc tatctcatca tgcctgaggg caaggcggtg cgctacggca ttggtgttgg ccgccgtggc tttgaatggt ctggcacggc ccgtgtggcg atgaagcgcg aatggccgac ctggacgccg ccttccgcca tgatcaagcg ccagccggaa ttggccaaat atcgcaatgg catggatccg ggcctgcgca atccgctcgg cgcgcgtgcg ctctatctct acaacaaggg cggggatacg ggctatcgcc tgcacggaac gccggaatgg tggtccatcg gcaaagccat gtcttccggc tgcatccgtc tgatgaacca ggacattatc gatctctata accgtgtcga acagggcgcg aaggttgtgg tgaaacagta a

Details for BrmeA.18116.a.A1.GE33704

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDSRVLKLT AALFLGAALA GCSTAGGSFY TASYSSVSDA GYAIPAIPSE KIPAQYRRQV VKYATDEKPG TIIVDTREKF LYLIMPEGKA VRYGIGVGRR GFEWSGTARV AMKREWPTWT PPSAMIKRQP ELAKYRNGMD PGLRNPLGAR ALYLYNKGGD TGYRLHGTPE WWSIGKAMSS GCIRLMNQDI IDLYNRVEQG AKVVVKQ

Validated NT Sequence

ttngtttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggattct cgcgttctca agctgacagc ggctcttttt cttggagcag cacttgctgg ttgctccaca gccggtggtt ctttctacac ggcgtcttac tcgtcagtca gcgatgctgg ttacgccatc ccggcaatcc cgagcgaaaa gataccggcg caatatcgcc gtcaggttgt aaaatatgcg acggacgaaa agccgggcac catcattgtc gatacgcgtg agaaatttct ctatctcatc atgcctgagg gcaaggcggt gcgctacggc attggtgttg gccgccgtgg ctttgaatgg tctggcacgg cccgtgtggc gatgaagcgc gaatggccga cctggacgcc gccttccgcc atgatcaagc gccagccgga attggccaaa tatcgcaatg gcatggatcc gggcctgcgc aatccgctcg gcgcgcgtgc gctctatctc tacaacaagg gcggggatac gggctatcgc ctgcacggaa cgccggaatg gtggtccatc ggcaaagcca tgtcttccgg ctgcatccgt ctgatgaacc aggacattat cgatctctat aaccgtgtcg aacagggcgc gaaggttgtg gtgaaacagt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctganttg gctgctgcca ccgctnannn nnnnaannta gnaanaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggattctcgc gttctcaagc tgacagcggc tctttttctt ggagcagcac ttgctggttg ctccacagcc ggtggttctt tctacacggc gtcttactcg tcagtcagcg atgctggtta cgccatcccg gcaatcccga gcgaaaagat accggcgcaa tatcgccgtc aggttgtaaa atatgcgacg gacgaaaagc cgggcaccat cattgtcgat acgcgtgaga aatttctcta tctcatcatg cctgagggca aggcggtgcg ctacggcatt ggtgttggcc gccgtggctt tgaatggtct ggcacggccc gtgtggcgat gaagcgcgaa tggccgacct ggacgccgcc ttccgccatg atcaagcgcc agccggaatt ggccaaatat cgcaatggca tggatccggg cctgcgcaat ccgctcggcg cgcgtgcgct ctatctctac aacaagggcg gggatacggg ctatcgcctg cacggaacgc cggaatggtg gtccatcggc aaagccatgt cttccggctg catccgtctg atgaaccagg acattatcga tctctataac cgtgtcgaac agggcgcgaa ggttgtggtg aaacagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa