SSGCID: Target Page

BrmeA.18114.b

L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16)

CENTER ID:	BrmeA.18114.b
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18114.b.B1.GE36710	Full length( BrmeA.18114.b )		1	454

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18114.b.B2.PW39356	AlphaFold domain		50	450

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_2510
BV-BRC:	fig\|224914.11.peg.2510
RefSeq:	NP_541201.1
UniProt:	Q8YDF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRWRATSRRN GGQHPDFVSP ILNGRPIEKD TNLKMMNEIK ATGSPDSNAA LPLAGIKVLD LSRVLAGPWA TMSLADMGAE VWKIENIQGG DDTRAWSVPN YKGASTYFLC ANRGKKSLAL DLKSREGLEI IHELAKQADV VVENFRSGTV ERLKIDYESL KALNPGIVYC SISGYGQTGP EAQRPGYDFV VQAESGLMSI TGQIDGEPTR IGVAMTDIVA GMVATQSVLA ALYQRKTTGL GQYIDVSLYE CALNTLINVG SAHLNGGHVP ARFGNAHPTV VPYQIFECSD GAFALAVGND RQFAILCERI IDLPELAADE RFKTASGRAL NRAALIPPMA ERFRTNTRQH WMSACLKMGV PAGQVKTVPE AFESPNVKAR QVVQKLESAH LGPISLVRPA QGLKAQENAA YKAPPMLGED SASVLGDVLG LDGNKLADLI AAGVIYQYQP KDCE

NT Sequence

ATGAGGTGGC GTGCCACTTC CCGGAGGAAT GGCGGGCAGC ATCCTGATTT TGTTTCACCA ATTTTGAACG GGCGACCGAT CGAGAAGGAT ACGAATTTGA AAATGATGAA TGAAATAAAG GCGACGGGCT CGCCCGACAG CAATGCAGCC TTGCCGCTTG CCGGGATAAA GGTTCTGGAC CTGTCGCGGG TGCTTGCCGG ACCCTGGGCG ACGATGAGCC TTGCCGACAT GGGAGCCGAG GTCTGGAAGA TTGAGAATAT CCAGGGCGGC GACGATACCC GCGCCTGGTC GGTGCCCAAT TACAAGGGGG CGAGCACCTA TTTCCTGTGC GCCAATCGCG GCAAGAAAAG CCTGGCTCTC GATCTGAAAT CCCGTGAAGG CCTGGAAATC ATCCATGAAC TGGCCAAGCA GGCCGATGTG GTTGTCGAGA ACTTTCGTTC AGGGACGGTC GAACGGCTGA AGATCGATTA TGAAAGCCTG AAAGCGCTCA ATCCCGGCAT CGTCTATTGC TCGATTTCCG GTTATGGCCA GACCGGCCCG GAGGCGCAGC GACCGGGTTA TGATTTCGTC GTGCAGGCGG AATCCGGCCT GATGTCGATT ACGGGGCAGA TCGATGGAGA GCCGACGCGC ATCGGCGTTG CCATGACGGA TATCGTTGCG GGCATGGTTG CCACGCAATC GGTCCTCGCC GCCCTTTATC AGCGCAAGAC GACGGGGCTT GGTCAATATA TCGACGTTTC ACTTTATGAA TGCGCGCTCA ATACATTGAT CAATGTCGGC AGCGCGCATC TGAATGGGGG GCATGTTCCC GCCCGCTTCG GCAATGCCCA TCCGACGGTC GTTCCCTACC AGATTTTTGA ATGTTCCGAC GGTGCGTTCG CGCTGGCGGT CGGCAATGAT CGCCAGTTTG CCATTCTTTG CGAACGGATC ATCGATCTGC CCGAACTGGC CGCAGACGAG CGCTTCAAAA CGGCAAGCGG TCGTGCCTTA AACCGCGCCG CGCTGATCCC GCCAATGGCG GAACGCTTTC GAACCAATAC CCGCCAGCAC TGGATGTCCG CATGCCTGAA GATGGGTGTG CCCGCAGGGC AGGTGAAAAC GGTGCCGGAG GCCTTTGAAA GCCCGAACGT GAAGGCGCGG CAGGTGGTGC AGAAACTGGA AAGCGCGCAT CTGGGCCCCA TTTCGCTGGT GCGGCCCGCA CAGGGGCTGA AAGCGCAGGA AAACGCCGCC TACAAGGCGC CGCCGATGCT TGGTGAAGAC AGTGCCTCTG TGCTCGGAGA TGTTCTCGGA CTGGATGGAA ACAAGCTGGC CGATCTGATC GCGGCCGGCG TCATTTATCA ATATCAGCCG AAGGATTGCG AA

Details for BrmeA.18114.b.B1.GE36710

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	51kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	89

Validated AA Sequence

MMNEIKATGS PDSNAALPLA GIKVLDLSRV LAGPWATMSL ADMGAEVWKI ENIQGGDDTR AWSVPNYKGA STYFLCANRG KKSLALDLKS REGLEIIHEL AKQADVVVEN FRSGTVERLK IDYESLKALN PGIVYCSISG YGQTGPEAQR PGYDFVVQAE SGLMSITGQI DGEPTRIGVA MTDIVAGMVA TQSVLAALYQ RKTTGLGQYI DVSLYECALN TLINVGSAHL NGGHVPARFX NAHPTVVPYQ IFECSDGAFX LAVGNDRQFA ILCERIIDLP ELAADERFKT ASGRAXXRAA LIPPXXERFR TNTRQHWXSA CLKMGVPAGQ VKTVPEAFES PNVKARQVVQ KLESAHXGPI XXVRPAQGLK AQENAAYKAP PMLGEDSASV LGDVLGLDGN KLADLIAAGV IY

Validated NT Sequence

gataaatgac gccggccgcg atcagatcgg ccagcttgtt tccatccagt ccgagaacat ctccgagcac agaggcactg tcttcaccaa gcatcggcgg cgccttgtag gcggcgtttt cctgcgcttt cagcccctgt gcgggccgca ccancnaaat ggggcccana tgcgcgcttt ccagtttctg caccacctgc cgcgccttca cgttcgggct ttcaaaggcc tccggcaccg ttttcacctg ccctgcgggc acacccatct tcaggcatgc ngacntccag tgctggcggg tattggttcg aaagcgttcc nccnttggcg ggatcagcgc ggcgcggntt anggcacgac cgcttgccgt tttgaancgc tcgtctgcgg ccagttcggg cagatcgatg atccgttcgc aaagaatggc aaactggcga tcattgccga ccgccagnnc gaacgcaccg tcggaacatt caaaaatctg gtagggaacg accgtcggat gggcattgnc gaagcgggcg ggaacatgcc ccccattcag atgcgcgctg ccgacattga tcaatgtatt gagcgcgcat tcataaagtg aaacgtcgat atattgacca agccccgtcg tcttgcgctg ataaagggcg gcgaggaccg attgcgtggc aaccatgccc gcaacgatat ccgtcatggc aacgccgatg cgcgtcggct ctccatcgat ctgccccgta atcgacatca ggccggattc cgcctgcacg acgaaatcat aacccggtcg ctgcgcctcc gggccggtct ggccataacc ggaaatcgag caatagacga tgccgggatt gagcgctttc aggctttcat aatcgatctt cagccgttcg accgtccctg aacgaaagtt ctcgacaacc acatcggcct gcttggccag ttcatggatg atttccaggc cttcacggga tttcagatcg agagccaggc ttttcttgcc gcgattggcg cacaggaaat aggtgctcgc ccccttgtaa ttgggcaccg accaggcgcg ggtatcgtcg ccgccctgga tattctcaat cttccagacc tcggctccca tgtcggcaag gctcatcgtc gcccagggtc cggcaagcac ccgcgacagg tccagaacct ttatcccggc aagcggcaag gctgcattgc tgtcgggcga gcccgtcgcc tttatttcat tcatcatttt caaattcgta tccttctcga tcggtcgccc gttcaaaatt ggtgaaacaa aatcaggatg ctgcccgcca ttcctccggg aagtggcacg cca

Expected Protein Sequence

MAHHHHHHMR WRATSRRNGG QHPDFVSPIL NGRPIEKDTN LKMMNEIKAT GSPDSNAALP LAGIKVLDLS RVLAGPWATM SLADMGAEVW KIENIQGGDD TRAWSVPNYK GASTYFLCAN RGKKSLALDL KSREGLEIIH ELAKQADVVV ENFRSGTVER LKIDYESLKA LNPGIVYCSI SGYGQTGPEA QRPGYDFVVQ AESGLMSITG QIDGEPTRIG VAMTDIVAGM VATQSVLAAL YQRKTTGLGQ YIDVSLYECA LNTLINVGSA HLNGGHVPAR FGNAHPTVVP YQIFECSDGA FALAVGNDRQ FAILCERIID LPELAADERF KTASGRALNR AALIPPMAER FRTNTRQHWM SACLKMGVPA GQVKTVPEAF ESPNVKARQV VQKLESAHLG PISLVRPAQG LKAQENAAYK APPMLGEDSA SVLGDVLGLD GNKLADLIAA GVIYQYQPKD CE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggt ggcgtgccac ttcccggagg aatggcgggc agcatcctga ttttgtttca ccaattttga acgggcgacc gatcgagaag gatacgaatt tgaaaatgat gaatgaaata aaggcgacgg gctcgcccga cagcaatgca gccttgccgc ttgccgggat aaaggttctg gacctgtcgc gggtgcttgc cggaccctgg gcgacgatga gccttgccga catgggagcc gaggtctgga agattgagaa tatccagggc ggcgacgata cccgcgcctg gtcggtgccc aattacaagg gggcgagcac ctatttcctg tgcgccaatc gcggcaagaa aagcctggct ctcgatctga aatcccgtga aggcctggaa atcatccatg aactggccaa gcaggccgat gtggttgtcg agaactttcg ttcagggacg gtcgaacggc tgaagatcga ttatgaaagc ctgaaagcgc tcaatcccgg catcgtctat tgctcgattt ccggttatgg ccagaccggc ccggaggcgc agcgaccggg ttatgatttc gtcgtgcagg cggaatccgg cctgatgtcg attacggggc agatcgatgg agagccgacg cgcatcggcg ttgccatgac ggatatcgtt gcgggcatgg ttgccacgca atcggtcctc gccgcccttt atcagcgcaa gacgacgggg cttggtcaat atatcgacgt ttcactttat gaatgcgcgc tcaatacatt gatcaatgtc ggcagcgcgc atctgaatgg ggggcatgtt cccgcccgct tcggcaatgc ccatccgacg gtcgttccct accagatttt tgaatgttcc gacggtgcgt tcgcgctggc ggtcggcaat gatcgccagt ttgccattct ttgcgaacgg atcatcgatc tgcccgaact ggccgcagac gagcgcttca aaacggcaag cggtcgtgcc ttaaaccgcg ccgcgctgat cccgccaatg gcggaacgct ttcgaaccaa tacccgccag cactggatgt ccgcatgcct gaagatgggt gtgcccgcag ggcaggtgaa aacggtgccg gaggcctttg aaagcccgaa cgtgaaggcg cggcaggtgg tgcagaaact ggaaagcgcg catctgggcc ccatttcgct ggtgcggccc gcacaggggc tgaaagcgca ggaaaacgcc gcctacaagg cgccgccgat gcttggtgaa gacagtgcct ctgtgctcgg agatgttctc ggactggatg gaaacaagct ggccgatctg atcgcggccg gcgtcattta tcaatatcag ccgaaggatt gcgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BrmeA.18114.b.B2.PW39356

PURIFICATION DATe:	12/17/2024
CONCENTRATION:	20.7mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHAL PLAGIKVLDL SRVLAGPWAT MSLADMGAEV WKIENIQGGD DTRAWSVPNY KGASTYFLCA NRGKKSLALD LKSREGLEII HELAKQADVV VENFRSGTVE RLKIDYESLK ALNPGIVYCS ISGYGQTGPE AQRPGYDFVV QAESGLMSIT GQIDGEPTRI GVAMTDIVAG MVATQSVLAA LYQRKTTGLG QYIDVSLYEC ALNTLINVGS AHLNGGHVPA RFGNAHPTVV PYQIFECSDG AFALAVGNDR QFAILCERII DLPELAADER FKTASGRALN RAALIPPMAE RFRTNTRQHW MSACLKMGVP AGQVKTVPEA FESPNVKARQ VVQKLESAHL GPISLVRPAQ GLKAQENAAY KAPPMLGEDS ASVLGDVLGL DGNKLADLIA AGVIYQYQP

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCTTGC CGCTTGCCGG GATAAAGGTT CTGGACCTGT CGCGGGTGCT TGCCGGACCC TGGGCGACGA TGAGCCTTGC CGACATGGGA GCCGAGGTCT GGAAGATTGA GAATATCCAG GGCGGCGACG ATACCCGCGC CTGGTCGGTG CCCAATTACA AGGGGGCGAG CACCTATTTC CTGTGCGCCA ATCGCGGCAA GAAAAGCCTG GCTCTCGATC TGAAATCCCG TGAAGGCCTG GAAATCATCC ATGAACTGGC CAAGCAGGCC GATGTGGTTG TCGAGAACTT TCGTTCAGGG ACGGTCGAAC GGCTGAAGAT CGATTATGAA AGCCTGAAAG CGCTCAATCC CGGCATCGTC TATTGCTCGA TTTCCGGTTA TGGCCAGACC GGCCCGGAGG CGCAGCGACC GGGTTATGAT TTCGTCGTGC AGGCGGAATC CGGCCTGATG TCGATTACGG GGCAGATCGA TGGAGAGCCG ACGCGCATCG GCGTTGCCAT GACGGATATC GTTGCGGGCA TGGTTGCCAC GCAATCGGTC CTCGCCGCCC TTTATCAGCG CAAGACGACG GGGCTTGGTC AATATATCGA CGTTTCACTT TATGAATGCG CGCTCAATAC ATTGATCAAT GTCGGCAGCG CGCATCTGAA TGGGGGGCAT GTTCCCGCCC GCTTCGGCAA TGCCCATCCG ACGGTCGTTC CCTACCAGAT TTTTGAATGT TCCGACGGTG CGTTCGCGCT GGCGGTCGGC AATGATCGCC AGTTTGCCAT TCTTTGCGAA CGGATCATCG ATCTGCCCGA ACTGGCCGCA GACGAGCGCT TCAAAACGGC AAGCGGTCGT GCCTTAAACC GCGCCGCGCT GATCCCGCCA ATGGCGGAAC GCTTTCGAAC CAATACCCGC CAGCACTGGA TGTCCGCATG CCTGAAGATG GGTGTGCCCG CAGGGCAGGT GAAAACGGTG CCGGAGGCCT TTGAAAGCCC GAACGTGAAG GCGCGGCAGG TGGTGCAGAA ACTGGAAAGC GCGCATCTGG GCCCCATTTC GCTGGTGCGG CCCGCACAGG GGCTGAAAGC GCAGGAAAAC GCCGCCTACA AGGCGCCGCC GATGCTTGGT GAAGACAGTG CCTCTGTGCT CGGAGATGTT CTCGGACTGG ATGGAAACAA GCTGGCCGAT CTGATCGCGG CCGGCGTCAT TTATCAATAT CAGCCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T