BrmeA.18113.c

Epi-inositol hydrolase (EC 3.7.1.-)

CENTER ID: BrmeA.18113.c
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.18113.c.B1.GE36709 Full length( BrmeA.18113.c ) 1 607
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2884
BV-BRC: fig|224914.11.peg.2884
RefSeq: NP_541549.1
RefSeq: WP_011005644.1
UniProt: Q8YCF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTVRLTAAQ ALVRYLANQM TPEGDTFIAG VWAIFGHGNV AGLGEALYRA REHMPTWRGH NEQTMAHAAI AYTKQLGRRR ACAVTSSIGP GATNMVTAAA LAHVNRLPVL FVPGDVFANR GPDPVLQQIE DFNDGTMTVN DCFRPVSRYF DRITRPEQLL TALPRAFRTM TDPADCGPVT LAFCQDVQAQ AYDWPEEFFT PKVWHWRRPP PDERELETAA AAIKTALKPV IVAGGGVHYS GAHEVLRAFV ETHGIPVIET QAGKSALPWD HPLNFGPVGV TGADSANAVA AEADLVIGVG TRFQDFTTGS WALFRHPGRR LLSLNVQPYD GAKHNSLPLV ADAKIGLEAL SGAIGAYRRQ AVDASLKNRW FAAADRFTAA PSDGNALPTD MQVIGAVQRQ ARENSVVMCA AGTMPGELHQ LWKAGRPMSY HMEYGFSCMG YEIAGGLGIK MAEPDRDVIV MVGDGSYMMA NSELATSIMM GQKITVVVTD NRGFGCISRL QMATGGAEFN NLLDHAAHVN PSQIDFAAHA AAMGADAKKV GSIRELEGAL AEARDSLRTT VIVIDTDPYP TPETGGWWWD VAVPEVSERD EVRAARKNYE AQIKERN
NT Sequence
ATGAAAACCG TTCGACTGAC CGCCGCGCAG GCACTGGTGC GCTATCTGGC CAACCAGATG ACGCCGGAGG GCGACACATT CATCGCGGGC GTCTGGGCCA TTTTCGGGCA CGGCAATGTG GCGGGGCTGG GCGAGGCGCT CTACCGCGCT CGCGAGCATA TGCCCACCTG GCGCGGCCAT AACGAGCAGA CCATGGCCCA TGCGGCCATC GCCTATACCA AGCAGCTTGG CCGCAGGCGC GCCTGCGCCG TCACCTCCTC CATCGGCCCC GGCGCAACCA ATATGGTGAC AGCGGCAGCA CTTGCCCATG TGAACCGCCT GCCCGTTCTG TTCGTTCCGG GCGATGTCTT CGCCAATCGC GGTCCCGATC CAGTTCTCCA GCAGATCGAG GATTTCAACG ACGGCACCAT GACCGTCAAC GACTGTTTCC GCCCGGTAAG CCGTTATTTC GACCGTATCA CCCGGCCGGA ACAGCTTCTC ACCGCCCTGC CTCGCGCCTT TCGCACCATG ACCGACCCAG CCGATTGCGG CCCGGTGACG CTCGCCTTCT GTCAGGATGT GCAGGCGCAA GCCTATGACT GGCCGGAAGA ATTCTTCACG CCCAAGGTCT GGCACTGGCG CCGCCCGCCG CCGGACGAGC GGGAACTGGA AACCGCTGCC GCCGCCATCA AAACCGCGCT GAAGCCCGTC ATTGTGGCGG GCGGCGGCGT GCATTATTCC GGCGCGCATG AGGTGCTGCG CGCTTTTGTA GAAACACATG GCATCCCCGT TATTGAAACA CAGGCGGGCA AATCCGCACT GCCATGGGAC CATCCGCTGA ATTTCGGTCC TGTCGGCGTG ACCGGCGCTG ACAGCGCCAA TGCGGTTGCG GCAGAAGCCG ATCTGGTGAT CGGTGTCGGC ACACGCTTTC AGGACTTCAC CACCGGCTCA TGGGCCCTGT TCAGGCATCC GGGGCGCAGG CTTCTTTCGC TCAATGTACA GCCCTATGAC GGAGCCAAGC ATAATTCTCT GCCGCTGGTA GCAGATGCGA AGATTGGGCT TGAGGCACTC TCCGGGGCCA TTGGCGCTTA CAGACGCCAG GCCGTCGATG CCAGCCTCAA AAACAGATGG TTTGCCGCTG CCGACCGTTT CACCGCCGCG CCCAGCGACG GCAATGCGCT ACCGACCGAC ATGCAGGTTA TCGGTGCGGT GCAGCGGCAA GCGCGCGAAA ATTCCGTTGT CATGTGCGCG GCAGGCACCA TGCCGGGTGA GTTGCATCAG CTCTGGAAGG CCGGGCGCCC CATGTCCTAC CACATGGAAT ATGGCTTTTC CTGCATGGGC TATGAGATTG CCGGCGGCCT TGGCATCAAG ATGGCCGAAC CGGACCGCGA CGTCATCGTG ATGGTGGGCG ACGGTTCCTA TATGATGGCC AATTCGGAAC TGGCCACCAG CATCATGATG GGGCAGAAAA TCACCGTCGT CGTGACCGAC AATCGCGGTT TCGGCTGCAT CAGCCGGCTG CAAATGGCGA CCGGCGGCGC GGAATTCAAC AATCTGCTCG ACCATGCCGC CCATGTGAAC CCGTCGCAGA TCGATTTTGC CGCGCACGCC GCCGCCATGG GTGCGGATGC GAAAAAGGTC GGCTCCATCC GCGAACTGGA GGGCGCACTG GCCGAAGCAC GCGACAGCCT GCGCACGACC GTCATTGTTA TCGACACCGA CCCTTACCCC ACGCCGGAAA CCGGCGGCTG GTGGTGGGAT GTCGCCGTAC CGGAAGTCTC GGAACGCGAT GAGGTGCGCG CCGCACGCAA AAACTATGAA GCCCAGATCA AAGAAAGAAA C
Details for BrmeA.18113.c.B1.GE36709
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 35
Validated AA Sequence
MQVIGAVQRQ ARENSVVMCA AGTMPGELHQ LWKAXRPMSY HMEYGFSCMG YEIAGGLGIK MAEPDRDVIV MVGDGSYMMA NSELATSIMM GQKITVVVTD NRGFGCISRL QMATGGAEFN NLLDHAAHVN PSQIDFAAHA AAMGADAKKV GSIRELEGAL AEARDSLRTT VIVIDTDPYP TPETGGWWWD VAVPEVSERD EVRAARKNYE AQIKERN
Validated NT Sequence
ttcgggcttt gttagcagcc ggatcctatc ttactcactt agtttctttc tttgatctgg gcttcatagt ttttgcgtgc ggcgcgcacc tcatcgcgtt ccgagacttc cggtacggcg acatcccacc accagccgcc ggtttccggc gtggggtaag ggtcggtgtc gataacaatg acggtcgtgc gcaggctgtc gcgtgcttcg gccagtgcgc cctccagttc gcggatggag ccgacctttt tcgcatccgc acccatggcg gcggcgtgcg cggcaaaatc gatctgcgac gggttcacat gggcggcatg gtcgagcaga ttgttgaatt ccgcgccgcc ggtcgccatt tgcagccggc tgatgcancc gaaaccgcga ttgtcggtca cgacnacggt gattttctgc cccatcatga tgctggtggc cagttccgaa ttggccatca tatangaacc gtcncccacc atcacgatga cgtcncggtc cggttcggcc atcttgatgc caaggccgcc ggcaatctca tagcccatgc aggaaaagcc atattccatg tggtangaca tggggcgccn ngccttccag agctgatgca actcacccgg catggtgcct gccgcgcaca tgacaacgga attttcgcgc gcttgccgct gcaccgcacc gataacctgc atgtcggtcg gtagcgcatt gccgtcgctg ggcgcggcgg tgaaacgntc ggcagcgnca aaccatctgt ttttgaggct ggcatcnacg gcctggcgtc tgtaagcgcc aatgnccccn gagagtgcct caagcccaat cttcgcatct gctaccagcg gcaganaatt atgcttggct ccgtcatang gntgtacatt gagcgaaaga agcctgcgcc ccngatgcct gaacangnnc natgagcnnn tggtgaantc ctgaangcgt gtgcnacnng atcaccnnat cggcttcngc nncnncngca nnnnngctgn cancnnnnna nnncgnnnng ncnnnnngcg nngnnnntgn nantnngnnn tgcnncnnnn ttnannnngg nnnnngnnnn nnnnnnnnnn ngcannnnnn nnnnnnnnnn nnnnnnnnnn nnnttna
Expected Protein Sequence
MAHHHHHHMK TVRLTAAQAL VRYLANQMTP EGDTFIAGVW AIFGHGNVAG LGEALYRARE HMPTWRGHNE QTMAHAAIAY TKQLGRRRAC AVTSSIGPGA TNMVTAAALA HVNRLPVLFV PGDVFANRGP DPVLQQIEDF NDGTMTVNDC FRPVSRYFDR ITRPEQLLTA LPRAFRTMTD PADCGPVTLA FCQDVQAQAY DWPEEFFTPK VWHWRRPPPD ERELETAAAA IKTALKPVIV AGGGVHYSGA HEVLRAFVET HGIPVIETQA GKSALPWDHP LNFGPVGVTG ADSANAVAAE ADLVIGVGTR FQDFTTGSWA LFRHPGRRLL SLNVQPYDGA KHNSLPLVAD AKIGLEALSG AIGAYRRQAV DASLKNRWFA AADRFTAAPS DGNALPTDMQ VIGAVQRQAR ENSVVMCAAG TMPGELHQLW KAGRPMSYHM EYGFSCMGYE IAGGLGIKMA EPDRDVIVMV GDGSYMMANS ELATSIMMGQ KITVVVTDNR GFGCISRLQM ATGGAEFNNL LDHAAHVNPS QIDFAAHAAA MGADAKKVGS IRELEGALAE ARDSLRTTVI VIDTDPYPTP ETGGWWWDVA VPEVSERDEV RAARKNYEAQ IKERN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa ccgttcgact gaccgccgcg caggcactgg tgcgctatct ggccaaccag atgacgccgg agggcgacac attcatcgcg ggcgtctggg ccattttcgg gcacggcaat gtggcggggc tgggcgaggc gctctaccgc gctcgcgagc atatgcccac ctggcgcggc cataacgagc agaccatggc ccatgcggcc atcgcctata ccaagcagct tggccgcagg cgcgcctgcg ccgtcacctc ctccatcggc cccggcgcaa ccaatatggt gacagcggca gcacttgccc atgtgaaccg cctgcccgtt ctgttcgttc cgggcgatgt cttcgccaat cgcggtcccg atccagttct ccagcagatc gaggatttca acgacggcac catgaccgtc aacgactgtt tccgcccggt aagccgttat ttcgaccgta tcacccggcc ggaacagctt ctcaccgccc tgcctcgcgc ctttcgcacc atgaccgacc cagccgattg cggcccggtg acgctcgcct tctgtcagga tgtgcaggcg caagcctatg actggccgga agaattcttc acgcccaagg tctggcactg gcgccgcccg ccgccggacg agcgggaact ggaaaccgct gccgccgcca tcaaaaccgc gctgaagccc gtcattgtgg cgggcggcgg cgtgcattat tccggcgcgc atgaggtgct gcgcgctttt gtagaaacac atggcatccc cgttattgaa acacaggcgg gcaaatccgc actgccatgg gaccatccgc tgaatttcgg tcctgtcggc gtgaccggcg ctgacagcgc caatgcggtt gcggcagaag ccgatctggt gatcggtgtc ggcacacgct ttcaggactt caccaccggc tcatgggccc tgttcaggca tccggggcgc aggcttcttt cgctcaatgt acagccctat gacggagcca agcataattc tctgccgctg gtagcagatg cgaagattgg gcttgaggca ctctccgggg ccattggcgc ttacagacgc caggccgtcg atgccagcct caaaaacaga tggtttgccg ctgccgaccg tttcaccgcc gcgcccagcg acggcaatgc gctaccgacc gacatgcagg ttatcggtgc ggtgcagcgg caagcgcgcg aaaattccgt tgtcatgtgc gcggcaggca ccatgccggg tgagttgcat cagctctgga aggccgggcg ccccatgtcc taccacatgg aatatggctt ttcctgcatg ggctatgaga ttgccggcgg ccttggcatc aagatggccg aaccggaccg cgacgtcatc gtgatggtgg gcgacggttc ctatatgatg gccaattcgg aactggccac cagcatcatg atggggcaga aaatcaccgt cgtcgtgacc gacaatcgcg gtttcggctg catcagccgg ctgcaaatgg cgaccggcgg cgcggaattc aacaatctgc tcgaccatgc cgcccatgtg aacccgtcgc agatcgattt tgccgcgcac gccgccgcca tgggtgcgga tgcgaaaaag gtcggctcca tccgcgaact ggagggcgca ctggccgaag cacgcgacag cctgcgcacg accgtcattg ttatcgacac cgacccttac cccacgccgg aaaccggcgg ctggtggtgg gatgtcgccg taccggaagt ctcggaacgc gatgaggtgc gcgccgcacg caaaaactat gaagcccaga tcaaagaaag aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat