SSGCID: Target Page

BrmeA.18113.b

COG0028: Thiamine pyrophosphate-requiring enzymes

CENTER ID:	BrmeA.18113.b
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.18113.b.B1.GE36708	Full length( BrmeA.18113.b )		1	500

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_2654
BV-BRC:	fig\|224914.11.peg.2654
RefSeq:	NP_541329.1
UniProt:	Q8YD26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEAYGKLTG KPGICFVTRG PGATNASIGV HTAFQDSTPM LLFIGQVASD QVEREAFQEI DYRRMFGQMA KWVVQIDDAA RIPELVSQAF HRAVNGRPGP VVVALPEDML TSYAAVSDAP AYKQVAMHPG GEQLQAMRAL IEKAERPLMI VGGGGWTQDA VRDLRQFAEN MSLPVAASFR CQDMFDNTHP LYAGEMGTSI SARLVQRVKD SDLLVVVGAR LGEMTTQGYE LVTIPVPQQK LVHIHPGAEE LGRVYHADVP INAGMPAFCA AAAKLAPVAD PRWKAWADSA NADYRDNIKS PVISGEVQMG EVMEWLRAHL PADAIITNGA GNYSAWPHRF YQYRTYRSQL APTNGSMGYG VPAAVAAKLT APERTVVAFA GDGCFLMNGQ ELATAAQYGA NAIFIVVNNG MYGTIRMHQE RTYPARISGT ALANPDFAAL ARAYGLHGEV VEKTADFAPA FKRCEQSGKP ALIEIRIDPE ALSPKMSLSQ MREQGFAKQR

NT Sequence

ATGGCAGAGG CCTATGGCAA ACTGACGGGT AAGCCCGGCA TTTGCTTCGT CACGCGCGGC CCCGGCGCCA CCAATGCATC GATTGGCGTG CACACGGCTT TTCAGGATTC GACGCCCATG CTGCTCTTCA TCGGTCAGGT CGCAAGCGAT CAGGTCGAGC GTGAGGCATT TCAGGAAATC GATTATCGCC GCATGTTCGG CCAGATGGCC AAATGGGTCG TGCAGATTGA CGATGCCGCG CGCATTCCCG AACTGGTGAG CCAGGCATTC CATCGCGCGG TGAACGGGCG TCCCGGTCCG GTGGTGGTCG CCTTGCCGGA AGACATGCTG ACGTCCTATG CGGCGGTCAG CGATGCGCCT GCCTATAAGC AGGTGGCCAT GCATCCGGGT GGCGAACAGT TGCAGGCAAT GCGGGCGCTG ATCGAAAAGG CAGAGCGTCC GCTGATGATC GTCGGCGGCG GTGGCTGGAC ACAGGATGCC GTGCGCGACC TGCGCCAGTT TGCCGAAAAT ATGTCTCTGC CGGTTGCCGC ATCCTTCCGC TGTCAGGATA TGTTCGACAA TACGCATCCC CTTTATGCGG GCGAAATGGG GACCTCGATC AGTGCCAGGC TTGTCCAGCG GGTGAAGGAT TCCGACCTGC TTGTCGTGGT TGGCGCGCGC CTTGGTGAAA TGACGACACA GGGCTATGAA CTCGTCACCA TTCCGGTGCC GCAGCAGAAG CTTGTCCATA TCCATCCGGG CGCGGAGGAA CTGGGCCGGG TCTATCATGC CGATGTTCCG ATCAATGCCG GGATGCCTGC CTTCTGTGCT GCGGCTGCAA AGCTTGCGCC GGTTGCCGAT CCGCGCTGGA AGGCATGGGC CGACAGCGCC AATGCCGACT ATCGCGACAA TATCAAGTCG CCGGTCATTT CGGGCGAAGT GCAGATGGGC GAGGTGATGG AGTGGCTGCG CGCGCATCTG CCCGCCGATG CCATTATCAC CAATGGCGCG GGCAATTATT CCGCATGGCC GCATCGCTTT TACCAGTATC GCACCTATCG CAGCCAGTTG GCCCCGACCA ATGGCTCCAT GGGCTATGGT GTGCCGGCTG CTGTTGCGGC CAAGCTCACC GCGCCGGAGC GGACCGTGGT GGCTTTCGCG GGAGATGGCT GTTTCCTCAT GAACGGGCAG GAGCTTGCAA CTGCCGCGCA ATATGGCGCG AATGCCATTT TCATCGTCGT GAACAACGGC ATGTATGGCA CGATCCGTAT GCATCAGGAG CGCACCTATC CGGCGCGCAT TTCGGGTACG GCCCTGGCCA ATCCCGACTT TGCGGCGCTT GCGCGGGCCT ATGGGCTGCA TGGTGAAGTA GTGGAAAAGA CCGCTGATTT CGCACCCGCC TTCAAGCGGT GCGAACAGTC CGGCAAGCCG GCGCTGATCG AGATCAGGAT CGATCCCGAG GCACTGTCGC CCAAGATGAG CCTCAGCCAG ATGCGGGAGC AGGGTTTCGC GAAACAGCGC

Details for BrmeA.18113.b.B1.GE36708

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	62

Validated AA Sequence

Validated NT Sequence

ctananattt nnnttnnctt taagaaggag anataccatg gctcaccacc accaccacca tatggcagag gcctatggca aactgacggg taagcccggc atttgcttcg tcacgcgcgg ccccggcgcc accaatgcat cgattggcgt gcacacggct tttcaggatt cgacgcccat gctgctcttc atcggtcagg tcgcaagcga tcaggtcgag cgtgaggcat ttcaggaaat cgattatcgc cgcatgttcg gccagatggc caaatgggtc gtgcagattg acgatgccgc gcgcattccc gaactggtga gccaggcatt ccatcgcgcg gtgaacgggc gtcccggtcc ggtggtggtc gccttgccgg aagacatgct gacgtcctat gcggcggtca gcgatgcgcc tgcctataag caggtggcca tgcatccggg tggcgaacag ttgcaggcaa tgcgggcgct gatcgaaaag gcagagcgtc cgctgatgat cgtcggcggc ggtggctgga cacaggatgc cgtgcgcgac ctgcgccagt ttgccgaaaa tatgtctctg ccggttgccg catccttccg ctgtcaggat atgttcgaca atacgcatcc cctttatgcg ggcgaaatgg ggacctcgat cagtgccagg cttgtccagc gggtgaagga ttccgacctg cttgtcgtgn ttggcgcgcg ccttggtgaa atgacgacac agggctatga actcgtcacc attccggtgc cgcagcanaa gcttgtccat atccntccgg gcgcgganga actgggccgg gtctatcatg ncgatgttcc natcaatgcc gggatgcctg ccttctgtgc tgnggctgca aagcttgnnc cggttgncna tccncnctgg aagnnnnggg ccnacagcgc natgncnact atcnnnanat atcantcnnc ngtcatttcg ggcnaantgn nnangggcna gnnnangnan tggctgnnnn nnnca

Expected Protein Sequence

MAHHHHHHMA EAYGKLTGKP GICFVTRGPG ATNASIGVHT AFQDSTPMLL FIGQVASDQV EREAFQEIDY RRMFGQMAKW VVQIDDAARI PELVSQAFHR AVNGRPGPVV VALPEDMLTS YAAVSDAPAY KQVAMHPGGE QLQAMRALIE KAERPLMIVG GGGWTQDAVR DLRQFAENMS LPVAASFRCQ DMFDNTHPLY AGEMGTSISA RLVQRVKDSD LLVVVGARLG EMTTQGYELV TIPVPQQKLV HIHPGAEELG RVYHADVPIN AGMPAFCAAA AKLAPVADPR WKAWADSANA DYRDNIKSPV ISGEVQMGEV MEWLRAHLPA DAIITNGAGN YSAWPHRFYQ YRTYRSQLAP TNGSMGYGVP AAVAAKLTAP ERTVVAFAGD GCFLMNGQEL ATAAQYGANA IFIVVNNGMY GTIRMHQERT YPARISGTAL ANPDFAALAR AYGLHGEVVE KTADFAPAFK RCEQSGKPAL IEIRIDPEAL SPKMSLSQMR EQGFAKQR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aggcctatgg caaactgacg ggtaagcccg gcatttgctt cgtcacgcgc ggccccggcg ccaccaatgc atcgattggc gtgcacacgg cttttcagga ttcgacgccc atgctgctct tcatcggtca ggtcgcaagc gatcaggtcg agcgtgaggc atttcaggaa atcgattatc gccgcatgtt cggccagatg gccaaatggg tcgtgcagat tgacgatgcc gcgcgcattc ccgaactggt gagccaggca ttccatcgcg cggtgaacgg gcgtcccggt ccggtggtgg tcgccttgcc ggaagacatg ctgacgtcct atgcggcggt cagcgatgcg cctgcctata agcaggtggc catgcatccg ggtggcgaac agttgcaggc aatgcgggcg ctgatcgaaa aggcagagcg tccgctgatg atcgtcggcg gcggtggctg gacacaggat gccgtgcgcg acctgcgcca gtttgccgaa aatatgtctc tgccggttgc cgcatccttc cgctgtcagg atatgttcga caatacgcat cccctttatg cgggcgaaat ggggacctcg atcagtgcca ggcttgtcca gcgggtgaag gattccgacc tgcttgtcgt ggttggcgcg cgccttggtg aaatgacgac acagggctat gaactcgtca ccattccggt gccgcagcag aagcttgtcc atatccatcc gggcgcggag gaactgggcc gggtctatca tgccgatgtt ccgatcaatg ccgggatgcc tgccttctgt gctgcggctg caaagcttgc gccggttgcc gatccgcgct ggaaggcatg ggccgacagc gccaatgccg actatcgcga caatatcaag tcgccggtca tttcgggcga agtgcagatg ggcgaggtga tggagtggct gcgcgcgcat ctgcccgccg atgccattat caccaatggc gcgggcaatt attccgcatg gccgcatcgc ttttaccagt atcgcaccta tcgcagccag ttggccccga ccaatggctc catgggctat ggtgtgccgg ctgctgttgc ggccaagctc accgcgccgg agcggaccgt ggtggctttc gcgggagatg gctgtttcct catgaacggg caggagcttg caactgccgc gcaatatggc gcgaatgcca ttttcatcgt cgtgaacaac ggcatgtatg gcacgatccg tatgcatcag gagcgcacct atccggcgcg catttcgggt acggccctgg ccaatcccga ctttgcggcg cttgcgcggg cctatgggct gcatggtgaa gtagtggaaa agaccgctga tttcgcaccc gccttcaagc ggtgcgaaca gtccggcaag ccggcgctga tcgagatcag gatcgatccc gaggcactgt cgcccaagat gagcctcagc cagatgcggg agcagggttt cgcgaaacag cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat