BrmeA.17543.a

Mannose-1-phosphate guanylyltransferase (GDP) ManC (EC 2.7.7.22) / Mannose-6-phosphate isomerase ManC (EC 5.3.1.8)

CENTER ID: BrmeA.17543.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.17543.a.B1.GE36707 Full length( BrmeA.17543.a ) 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_1558
BV-BRC: fig|224914.11.peg.1558
RefSeq: NP_540312.1
UniProt: Q8YFW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPVVLAGGK GTRLWPMSRS QRPKQFLALT GELSLFQMTL KRLSDPNRYS SPIIITNADY RFLVAEQALE CGVELSDVIL EPMARNTAPA IAAAALIASK DEQTLIHVVA SDHNIEINDA YFQAVDTACN AARMGRLATF GITPTEPATG FGYIEAGELE ESGACAIKRF VEKPKEEVAR EMLAAGGFYW NSGMFLFRSD VFLDECRRYA PAVYEAAEAS VATAQRDLDF VRLNSAEFEK APDISVDYAI FEKTKLASVV PSPIHWSDLG SWDAVWKESK QDPSGNVARG PVSLHNTNNS LVLSEGVHVA VNGVDDLTIV ASEDALYVGK LSEAQGVGTI VKALMKQPAT AKLAEEHRTI YRPWGGYTHL LSGDRLQVKR LFIKPGKQLS LQKHHHRSEH WVVVRGVAEI VIDDKSLFLS ENESTYISQG AIHRVSNPGK ILLELIEVQT GAYLGEDDIV RFEDDFGRV
NT Sequence
ATGAGACCAG TAGTTTTGGC CGGCGGAAAA GGTACGAGAT TGTGGCCGAT GTCGCGCTCG CAACGCCCAA AACAGTTTCT GGCTCTGACT GGCGAACTCA GCCTCTTTCA GATGACTTTG AAACGATTGT CGGACCCGAA TCGCTATTCC TCGCCGATTA TCATAACAAA CGCTGACTAT CGGTTTCTGG TGGCGGAACA GGCATTAGAA TGTGGTGTCG AACTTTCGGA TGTCATTCTG GAGCCCATGG CTCGTAACAC GGCACCGGCG ATCGCCGCTG CGGCTTTGAT CGCTTCCAAG GATGAGCAGA CCCTTATTCA TGTTGTGGCA TCTGATCACA ATATTGAAAT AAACGATGCA TATTTTCAAG CTGTGGATAC GGCCTGCAAT GCTGCTCGTA TGGGACGTCT TGCCACTTTT GGCATTACGC CAACGGAACC AGCGACCGGT TTTGGTTACA TCGAAGCAGG CGAGTTGGAA GAAAGTGGAG CTTGTGCGAT TAAGCGCTTT GTGGAAAAAC CGAAGGAAGA AGTTGCGCGC GAAATGCTTG CTGCAGGCGG CTTCTACTGG AACTCGGGGA TGTTTTTGTT CCGTTCGGAC GTTTTTCTCG ACGAATGCAG GCGTTACGCA CCAGCAGTAT ATGAGGCTGC TGAGGCGTCC GTTGCAACTG CACAGCGGGA TCTCGATTTT GTGCGTCTGA ACAGCGCCGA ATTTGAGAAA GCTCCGGATA TTTCCGTTGA CTATGCGATT TTCGAGAAGA CAAAACTCGC TTCTGTTGTT CCGTCACCAA TCCACTGGTC GGATCTTGGA TCGTGGGATG CTGTCTGGAA GGAAAGCAAA CAGGATCCGT CGGGAAATGT TGCTCGCGGA CCTGTCAGCC TGCACAACAC CAATAATTCG CTGGTCTTGT CTGAAGGCGT TCATGTTGCG GTCAATGGTG TTGACGATCT CACGATTGTG GCCTCGGAAG ATGCGCTTTA TGTTGGCAAG CTCTCTGAAG CGCAAGGTGT AGGCACGATC GTAAAGGCGC TGATGAAACA ACCCGCCACG GCGAAGCTTG CTGAGGAGCA TCGGACCATC TATCGCCCTT GGGGCGGCTA CACCCATTTG CTTTCGGGTG ATCGGCTTCA GGTAAAGCGT CTGTTCATCA AGCCGGGCAA ACAGCTCTCG CTACAGAAGC ATCACCATCG TTCAGAGCAT TGGGTGGTTG TTCGGGGCGT GGCCGAAATC GTGATCGATG ATAAGAGCCT TTTCCTGTCG GAGAACGAAT CGACATATAT TTCTCAAGGT GCGATACACC GTGTTTCAAA TCCGGGTAAA ATTCTGCTCG AACTGATTGA AGTTCAAACT GGAGCTTATC TGGGCGAAGA CGATATTGTG CGCTTCGAGG ATGATTTCGG ACGTGTT
Details for BrmeA.17543.a.B1.GE36707
HARVESTED ON: 2/27/2013
SEQUENCED ON: 3/1/2013
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR PVVLAGGKGT RLWPMSRSQR PKQFLALTGE LSLFQMTLKR LSDPNRYSSP IIITNADYRF LVAEQALECG VELSDVILEP MARNTAPAIA AAALIASKDE QTLIHVVASD HNIEINDAYF QAVDTACNAA RMGRLATFGI TPTEPATGFG YIEAGELEES GACAIKRFVE KPKEEVAREM LAAGGFYWNS GMFLFRSDVF LDECRRYAPA VYEAAEASVA TAQRDLDFVR LNSAEFEKAP DISVDYAIFE KTKLASVVPS PIHWSDLGSW DAVWKESKQD PSGNVARGPV SLHNTXNSLV LSEGVHVAVN GVDDLTIVAS EDALYVGKLX EAQGVGTIVK ALMKQPATAK LAEEHRTIYR PWGGYTHLLS GDRLQVKRLF IKPGKQLSLQ KHHHRSEHWV VVRGVAEIVI DDKSLFLSEN ESTYISQGAI HRVSNPGKIL LELIEVQTGA YLGEDDIV
Validated NT Sequence
atggctcacc accaccacca ccatatgaga ccagtagttt tggccggcgg aaaaggtacg agattgtggc cgatgtcgcg ctcgcaacgc ccaaaacagt ttctggctct gactggcgaa ctcagcctct ttcagatgac tttgaaacga ttgtcggacc cgaatcgcta ttcctcgccg attatcataa caaacgctga ctatcggttt ctggtggcgg aacaggcatt agaatgtggt gtcgaacttt cggatgtcat tctggagccc atggctcgta acacggcacc ggcgatcgcc gctgcggctt tgatcgcttc caaggatgag cagaccctta ttcatgttgt ggcatctgat cacaatattg aaataaacga tgcatatttt caagctgtgg atacggcctg caatgctgct cgtatgggac gtcttgccac ttttggcatt acgccaacgg aaccagcgac cggttttggt tacatcgaag caggcgagtt ggaagaaagt ggagcttgtg cgattaagcg ctttgtggaa aaaccgaagg aagaagttgc gcgcgaaatg cttgctgcag gcggcttcta ctggaactcg gggatgtttt tgttccgttc ggacgttttt ctcgacgaat gcaggcgtta cgcaccagca gtatatgagg ctgctgaggc gtccgttgca actgcacagc gggatctcga ttttgtgcgt ctgaacagcg ccgaatttga gaaagctccg gatatttccg ttgactatgc gattttcgag aagacaaaac tcgcttctgt tgttccgtca ccaatccact ggtcggatct tggatcgtgg gatgctgtct ggaaggaaag caaacaggat ccgtcgggaa atgttgctcg cggacctgtc agcctgcaca acaccnataa ttcgctggtc ttgtctgaag gcgttcatgt tgcggtcaat ggtgttgacg atctcacgat tgtggcctcg gaagatgcgc tttatgttgg caagctctnt gaagcgcaag gtgtaggcac gatcgtaaag gcgctgatga aacaacccgc cacggcgaag cttgctgagg agcatcggac catctatcgc ccttggggcg gctacaccca tttgctttcg ggtgatcggc ttcaggtaaa gcgtctgttc atcaagccgg gcaaacagct ctcgctacag aagcatcacc atcgttcaga gcattgggtg gttgttcggg gcgtggccga aatcgtgatc gatgataaga gccttttcct gtcggagaac gaatcgacat atatttctca aggtgcgata caccgtgttt caaatccggg taaaattctg ctcgaactga ttgaagttca aactggagct tatctgggcg aagacgatat tgtgcg
Expected Protein Sequence
MAHHHHHHMR PVVLAGGKGT RLWPMSRSQR PKQFLALTGE LSLFQMTLKR LSDPNRYSSP IIITNADYRF LVAEQALECG VELSDVILEP MARNTAPAIA AAALIASKDE QTLIHVVASD HNIEINDAYF QAVDTACNAA RMGRLATFGI TPTEPATGFG YIEAGELEES GACAIKRFVE KPKEEVAREM LAAGGFYWNS GMFLFRSDVF LDECRRYAPA VYEAAEASVA TAQRDLDFVR LNSAEFEKAP DISVDYAIFE KTKLASVVPS PIHWSDLGSW DAVWKESKQD PSGNVARGPV SLHNTNNSLV LSEGVHVAVN GVDDLTIVAS EDALYVGKLS EAQGVGTIVK ALMKQPATAK LAEEHRTIYR PWGGYTHLLS GDRLQVKRLF IKPGKQLSLQ KHHHRSEHWV VVRGVAEIVI DDKSLFLSEN ESTYISQGAI HRVSNPGKIL LELIEVQTGA YLGEDDIVRF EDDFGRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagac cagtagtttt ggccggcgga aaaggtacga gattgtggcc gatgtcgcgc tcgcaacgcc caaaacagtt tctggctctg actggcgaac tcagcctctt tcagatgact ttgaaacgat tgtcggaccc gaatcgctat tcctcgccga ttatcataac aaacgctgac tatcggtttc tggtggcgga acaggcatta gaatgtggtg tcgaactttc ggatgtcatt ctggagccca tggctcgtaa cacggcaccg gcgatcgccg ctgcggcttt gatcgcttcc aaggatgagc agacccttat tcatgttgtg gcatctgatc acaatattga aataaacgat gcatattttc aagctgtgga tacggcctgc aatgctgctc gtatgggacg tcttgccact tttggcatta cgccaacgga accagcgacc ggttttggtt acatcgaagc aggcgagttg gaagaaagtg gagcttgtgc gattaagcgc tttgtggaaa aaccgaagga agaagttgcg cgcgaaatgc ttgctgcagg cggcttctac tggaactcgg ggatgttttt gttccgttcg gacgtttttc tcgacgaatg caggcgttac gcaccagcag tatatgaggc tgctgaggcg tccgttgcaa ctgcacagcg ggatctcgat tttgtgcgtc tgaacagcgc cgaatttgag aaagctccgg atatttccgt tgactatgcg attttcgaga agacaaaact cgcttctgtt gttccgtcac caatccactg gtcggatctt ggatcgtggg atgctgtctg gaaggaaagc aaacaggatc cgtcgggaaa tgttgctcgc ggacctgtca gcctgcacaa caccaataat tcgctggtct tgtctgaagg cgttcatgtt gcggtcaatg gtgttgacga tctcacgatt gtggcctcgg aagatgcgct ttatgttggc aagctctctg aagcgcaagg tgtaggcacg atcgtaaagg cgctgatgaa acaacccgcc acggcgaagc ttgctgagga gcatcggacc atctatcgcc cttggggcgg ctacacccat ttgctttcgg gtgatcggct tcaggtaaag cgtctgttca tcaagccggg caaacagctc tcgctacaga agcatcacca tcgttcagag cattgggtgg ttgttcgggg cgtggccgaa atcgtgatcg atgataagag ccttttcctg tcggagaacg aatcgacata tatttctcaa ggtgcgatac accgtgtttc aaatccgggt aaaattctgc tcgaactgat tgaagttcaa actggagctt atctgggcga agacgatatt gtgcgcttcg aggatgattt cggacgtgtt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat