SSGCID: Target Page

BrmeA.17389.a

Methionyl-tRNA formyltransferase (EC 2.1.2.9)

CENTER ID:	BrmeA.17389.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.17389.a.B1.GE36706	Full length( BrmeA.17389.a )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_2554
BV-BRC:	fig\|224914.11.peg.2554
RefSeq:	NP_541242.1
UniProt:	P64132

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVVFMGTPE FSVPILTAII GHGYEVVAAY TQPPRPAGRR GLELTRSPVH EKAEQFGIPV FTPKSLKGAE EQDVFASLEA DVAIVVAYGL LLPKAILDAP RLGCYNGHAS LLPRWRGAAP IQRAIMAGDA ETGMMIMKMD EGLDTGPVAM AEKVAITPDM TAGELHDRLS MIGADLMIRA LGALERESLA LQPQAEEGVT YAAKIDKAEA RIDWSKPAKD VHNSIRGLSP FPGAWCEMEI NGAVERVKLQ RSTLGEGSGA PGTVLDDRLT IACGEGAVRL ATLQRSGGKP LPAQEFLRGQ RVTKVL

NT Sequence

ATGCGTGTTG TTTTCATGGG GACGCCGGAA TTTTCCGTGC CGATCCTCAC CGCCATCATC GGTCACGGTT ATGAGGTCGT TGCAGCCTAT ACGCAGCCGC CGCGCCCGGC AGGCCGCAGA GGTCTGGAAC TCACCAGATC GCCGGTGCAT GAAAAAGCAG AGCAATTCGG CATTCCGGTT TTCACGCCGA AGAGCCTCAA GGGCGCGGAA GAGCAGGATG TTTTCGCAAG CCTTGAAGCC GATGTGGCGA TTGTCGTGGC CTATGGGCTT TTGCTGCCGA AGGCCATTCT CGATGCGCCG CGGCTTGGCT GCTATAACGG CCATGCGTCG CTTTTGCCGC GTTGGCGCGG TGCAGCGCCC ATCCAGCGCG CTATTATGGC AGGCGATGCG GAAACCGGCA TGATGATCAT GAAGATGGAT GAGGGGCTTG ATACCGGGCC TGTCGCCATG GCCGAAAAGG TGGCCATCAC GCCGGATATG ACGGCGGGCG AACTGCATGA TCGCCTGAGC ATGATTGGCG CTGACCTGAT GATCCGCGCG CTGGGCGCGC TTGAGCGCGA AAGCCTTGCC TTGCAGCCGC AGGCGGAAGA AGGCGTTACT TATGCCGCAA AGATCGACAA GGCGGAGGCC CGCATCGACT GGTCGAAGCC TGCAAAAGAC GTGCACAATA GCATTCGCGG CCTGTCACCC TTTCCGGGCG CGTGGTGCGA GATGGAAATC AACGGTGCTG TCGAGCGCGT GAAGTTGCAG CGTTCCACGC TGGGCGAAGG TTCGGGCGCG CCGGGCACGG TGCTGGATGA TCGCCTGACG ATTGCCTGCG GCGAGGGCGC GGTGCGGCTT GCCACGCTGC AACGTTCCGG CGGAAAACCC TTGCCTGCAC AAGAGTTTCT GCGTGGGCAG CGCGTCACGA AGGTTCTC

Details for BrmeA.17389.a.B1.GE36706

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR VVFMGTPEFS VPILTAIIGH GYEVVAAYTQ PPRPAGRRGL ELTRSPVHEK AEQFGIPVFT PKSLKGAEEQ DVFASLEADV AIVVAYGLLL PKAILDAPRL GCYNGHASLL PRWRGAAPIQ RAIMAGDAET GMMIMKMDEG LDTGPVAMAE KVAITPDMTA GELHDRLSMI GADLMIRALG ALERESLALQ PQAEEGVTYA AKIDKAEARI DWSKPAKDVH NSIRGLSPFP GAWCEMEING AVERVKLQRS TLGEGSGAPG TVLDDRLTIA CGEGAVRLAT LQRSGGKPLP AQEFLRGQRV TKVL

Validated NT Sequence

ctcacttaga gaaccttcgt gacgcgctgc ccacgcagaa actcttgtgc aggcaagggt tttccgccgg aacgttgcag cgtggcaagc cgcaccgcgc cctcgccgca ggcaatcgtc aggcgatcat ccagcaccgt gcccggcgcg cccgaacctt cgcccagcgt ggaacgctgc aacttcacgc gctcgacagc accgttgatt tccatctcgc accacgcgcc cggaaagggt gacaggccgc gaatgctatt gtgcacgtct tttgcaggct tcgaccagtc gatgcgggcc tccgccttgt cgatctttgc ggcataagta acgccttctt ccgcctgcgg ctgcaaggca aggctttcgc gctcaagcgc gcccagcgcg cggatcatca ggtcagcgcc aatcatgctc aggcgatcat gcagttcgcc cgccgtcata tccggcgtga tggccacctt ttcggccatg gcgacaggcc cggtatcaag cccctcatcc atcttcatga tcatcatgcc ggtttccgca tcgcctgcca taatagcgcg ctggatgggc gctgcaccgc gccaacgcgg caaaagcgac gcatggccgt tatagcagcc aagccgcggc gcatcgagaa tggccttcgg cagcaaaagc ccataggcca cgacaatcgc cacatcggct tcaaggcttg cgaaaacatc ctgctcttcc gcgcccttga ggctcttcgg cgtgaaaacc ggaatgccga attgctctgc tttttcatgc accggcgatc tggtgagttc cagacctctg cggcctgccg ggcgcggcgg ctgcgtatag gctgcaacga cctcataacc gtgaccgatg atggcggtga ggatcggcac ggaaaattcc ggcgtcccca tgaaaacaac acgcatatgg tggtggtggt ggtgagccat ggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg ttgttttcat ggggacgccg gaattttccg tgccgatcct caccgccatc atcggtcacg gttatgaggt cgttgcagcc tatacgcagc cgccgcgccc ggcaggccgc agaggtctgg aactcaccag atcgccggtg catgaaaaag cagagcaatt cggcattccg gttttcacgc cgaagagcct caagggcgcg gaagagcagg atgttttcgc aagccttgaa gccgatgtgg cgattgtcgt ggcctatggg cttttgctgc cgaaggccat tctcgatgcg ccgcggcttg gctgctataa cggccatgcg tcgcttttgc cgcgttggcg cggtgcagcg cccatccagc gcgctattat ggcaggcgat gcggaaaccg gcatgatgat catgaagatg gatgaggggc ttgataccgg gcctgtcgcc atggccgaaa aggtggccat cacgccggat atgacggcgg gcgaactgca tgatcgcctg agcatgattg gcgctgacct gatgatccgc gcgctgggcg cgcttgagcg cgaaagcctt gccttgcagc cgcaggcgga agaaggcgtt acttatgccg caaagatcga caaggcggag gcccgcatcg actggtcgaa gcctgcaaaa gacgtgcaca atagcattcg cggcctgtca ccctttccgg gcgcgtggtg cgagatggaa atcaacggtg ctgtcgagcg cgtgaagttg cagcgttcca cgctgggcga aggttcgggc gcgccgggca cggtgctgga tgatcgcctg acgattgcct gcggcgaggg cgcggtgcgg cttgccacgc tgcaacgttc cggcggaaaa cccttgcctg cacaagagtt tctgcgtggg cagcgcgtca cgaaggttct ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat