BrmeA.17368.b
ABC transporter, substrate-binding protein
| CENTER ID: | BrmeA.17368.b |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.17368.b.B2.GE36702 | Mature protein( BrmeA.17368.b ) | 23 | 368 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4N0Q
DEPOSITED: 10/2/2013
DETERMINATION: XRay
CLONE: BrmeA.17368.b.B2.GE36702
PROTEIN: BrmeA.17368.b.B2.PS01844
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|224914.11.peg.2132 |
| RefSeq: | NP_540847.1 |
| UniProt: | Q8YEE8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLKLLSSVA FAATIGFASA AYADITIGVI APLTGPVAAF GDQVKKGAET AVEVINKAGG IKGEKVVLKF ADDAGEPKQG VSAANQIVGD GIKFVVGLVT TGVAVPVSDV LSENGVLMVT PTATGPDLTA RGLENVFRTC GRDGQQAEVM ADYVLKNMKD KKVAVIHDKG AYGKGLADAF KAAINKGGIT EVHYDSVTPG DKDFSALVTK LKSAGAEVVY FGGYHAEGGL LSRQLHDAGM QALVLGGEGL SNTEYWAIGG TNAQGTLFTN AKDATKNPAA KDAIQALKAK NIPAEAFTMN AYAAVEVIKA GIERAGSTDD SAAVAKALHD GKPIETAIGT LTYSETGDLS SPSFDIFKWD DGKIVGLE
NT Sequence
ATGAACCTGA AACTTCTGTC CAGCGTGGCT TTTGCCGCTA CCATCGGCTT TGCCAGCGCC GCTTACGCAG ACATCACCAT CGGTGTGATT GCACCGCTGA CGGGCCCGGT TGCCGCTTTC GGCGATCAGG TGAAAAAGGG CGCGGAAACC GCCGTGGAAG TCATCAACAA GGCTGGCGGC ATCAAGGGTG AGAAAGTCGT CCTGAAGTTT GCCGACGATG CGGGCGAGCC GAAGCAGGGC GTTTCCGCTG CCAACCAGAT CGTTGGCGAT GGCATCAAGT TTGTCGTCGG CCTGGTTACG ACCGGCGTTG CCGTCCCCGT TTCCGACGTT TTGTCTGAAA ACGGCGTCCT CATGGTCACC CCGACGGCTA CCGGCCCGGA CCTGACCGCG CGCGGCCTGG AAAACGTGTT CCGTACCTGC GGCCGCGACG GCCAGCAGGC TGAAGTCATG GCTGATTACG TCCTGAAAAA CATGAAGGAC AAGAAGGTTG CCGTGATCCA CGACAAGGGC GCTTATGGCA AGGGTCTTGC CGATGCGTTC AAGGCGGCCA TCAACAAGGG CGGCATCACC GAAGTCCATT ATGATTCGGT CACACCGGGC GACAAGGATT TTTCCGCGCT CGTCACCAAG CTGAAGTCTG CCGGTGCTGA AGTGGTCTAT TTCGGCGGCT ACCATGCTGA AGGCGGTCTC CTGTCGCGCC AGCTCCACGA TGCTGGCATG CAGGCCCTGG TGCTCGGCGG CGAAGGCCTC TCCAACACCG AATATTGGGC AATCGGCGGC ACAAACGCAC AGGGTACGCT CTTCACCAAC GCCAAGGATG CCACGAAGAA CCCGGCTGCC AAGGACGCTA TCCAAGCCCT GAAGGCGAAG AACATCCCGG CTGAAGCTTT CACGATGAAC GCCTATGCGG CTGTGGAAGT CATCAAGGCC GGCATCGAAC GCGCCGGTTC GACTGACGAT TCCGCTGCCG TCGCCAAGGC GCTGCATGAC GGCAAGCCGA TCGAAACCGC AATCGGCACG CTGACCTATA GCGAAACCGG CGACCTTAGC TCGCCAAGCT TTGATATCTT CAAGTGGGAT GACGGCAAGA TCGTCGGTCT TGAA
Details for BrmeA.17368.b.B2.GE36702
| HARVESTED ON: | 2/27/2013 |
| SEQUENCED ON: | 2/2/2013 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 88 |
Validated AA Sequence
MAHHHHHHMA DITIGVIAPL TGPVAAFGDQ VKKGAETAVE VINKAGGIKG EKVVLKFADD AGEPKQGVSA ANQIVGDGIK FVVGLVTTGV AVPVSDVLSE NGVLMVTPTA TGPDLTARGL ENVFRTCGRD GQQAEVMADY VLKNMKDKKV AVIHDKGAYG KGLADAFKAA INKGGITEVH YDSVTPGDKD FSALVTKLKS AGAEVVYFGG YHAEGGLLSR QLHDAGMQAL VLGGEGLSNT EYWAIGGTNA QGTLFTNAKD ATKNPAAKDA IQALKAKNIP AEAFTMNAYA AVEVIKAGIE RAGSTDDSAA VAK
Validated NT Sequence
agaccgacga tcttgccgtc atcccacttg aagatatcaa agcttggcga gctaaggtcg ccggtttcgc tataggtcag cgtgccgatt gcggtttcga tcggcttgcc gtcatgcagc gccttggcga cggcagcgga atcgtcagtc gaaccggcgc gttcgatgcc ggccttgatg acttccacag ccgcataggc gttcatcgtg aaagcttcag ccgggatgtt cttcgccttc agggcttgga tagcgtcctt ggcagccggg ttcttcgtgg catccttggc gttggtgaag agcgtaccct gtgcgtttgt gccgccgatt gcccaatatt cggtgttgga gaggccttcg ccgccgagca ccagggcctg catgccagca tcgtggagct ggcgcgacag gagaccgcct tcagcatggt agccgccgaa atagaccact tcagcaccgg cagacttcag cttggtgacg agcgcggaaa aatccttgtc gcccggtgtg accgaatcat aatggacttc ggtgatgccg cccttgttga tggccgcctt gaacgcatcg gcaagaccct tgccataagc gcccttgtcg tggatcacgg caaccttctt gtccttcatg tttttcagga cgtaatcagc catgacttca gcctgctggc cgtcgcggcc gcaggtacgg aacacgtttt ccaggccgcg cgcggtcagg tccgggccgg tagccgtcgg ggtgaccatg aggacgccgt tttcagacaa aacgtcggaa acggggacgg caacgccggt cgtaaccagg ccgacgacaa acttgatgcc atcgccaacg atctggttgg cagcggaaac gccctgcttc ggctcgcccg catcgtcggc aaacttcagg acgactttct cacccttgat gccgccagcc ttgttgatga cttccacggc ggtttccgcg ccctttttca cctgatcgcc gaaagcggca accgggcccg tcagcggtgc aatcacaccg atggtga
Expected Protein Sequence
MAHHHHHHAD ITIGVIAPLT GPVAAFGDQV KKGAETAVEV INKAGGIKGE KVVLKFADDA GEPKQGVSAA NQIVGDGIKF VVGLVTTGVA VPVSDVLSEN GVLMVTPTAT GPDLTARGLE NVFRTCGRDG QQAEVMADYV LKNMKDKKVA VIHDKGAYGK GLADAFKAAI NKGGITEVHY DSVTPGDKDF SALVTKLKSA GAEVVYFGGY HAEGGLLSRQ LHDAGMQALV LGGEGLSNTE YWAIGGTNAQ GTLFTNAKDA TKNPAAKDAI QALKAKNIPA EAFTMNAYAA VEVIKAGIER AGSTDDSAAV AKALHDGKPI ETAIGTLTYS ETGDLSSPSF DIFKWDDGKI VGLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaca tcaccatcgg tgtgattgca ccgctgacgg gcccggttgc cgctttcggc gatcaggtga aaaagggcgc ggaaaccgcc gtggaagtca tcaacaaggc tggcggcatc aagggtgaga aagtcgtcct gaagtttgcc gacgatgcgg gcgagccgaa gcagggcgtt tccgctgcca accagatcgt tggcgatggc atcaagtttg tcgtcggcct ggttacgacc ggcgttgccg tccccgtttc cgacgttttg tctgaaaacg gcgtcctcat ggtcaccccg acggctaccg gcccggacct gaccgcgcgc ggcctggaaa acgtgttccg tacctgcggc cgcgacggcc agcaggctga agtcatggct gattacgtcc tgaaaaacat gaaggacaag aaggttgccg tgatccacga caagggcgct tatggcaagg gtcttgccga tgcgttcaag gcggccatca acaagggcgg catcaccgaa gtccattatg attcggtcac accgggcgac aaggattttt ccgcgctcgt caccaagctg aagtctgccg gtgctgaagt ggtctatttc ggcggctacc atgctgaagg cggtctcctg tcgcgccagc tccacgatgc tggcatgcag gccctggtgc tcggcggcga aggcctctcc aacaccgaat attgggcaat cggcggcaca aacgcacagg gtacgctctt caccaacgcc aaggatgcca cgaagaaccc ggctgccaag gacgctatcc aagccctgaa ggcgaagaac atcccggctg aagctttcac gatgaacgcc tatgcggctg tggaagtcat caaggccggc atcgaacgcg ccggttcgac tgacgattcc gctgccgtcg ccaaggcgct gcatgacggc aagccgatcg aaaccgcaat cggcacgctg acctatagcg aaaccggcga ccttagctcg ccaagctttg atatcttcaa gtgggatgac ggcaagatcg tcggtcttga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat