SSGCID: Target Page

BrmeA.17270.a

Trigger factor

CENTER ID:	BrmeA.17270.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.17270.a.A1.GE34622	full length		1	477

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.1200
RefSeq:	NP_539986.1
UniProt:	Q8YGT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVTETLNEG LKREIKVVVP AGDLEAKLAE RLETARGRAR INGFRPGKVP TAHLRKMYGK SFMAEIVNEI LNDSSRSILA ERNEKSATQP EVIMSEDEKE AEKVLDGKAD FVFSLNYEVL PAIEVKDFSK IAVTREVVDI SDEEVDEQVK RIASSTRTFE TKKGKAENED RVTIDYLGKL DGEPFEGGAD NDAQLVLGSG QFIPGFEEQL IGLKAGDEKV ITVTFPAEYG AAHLAGKEAT FDIKVKEVAK PNELVLDDET AKKLGIESLE RLRQVVREQI ESQYGQITRQ KVKRQILDAL DGDYQFETPQ KLVDAEFNNI WQQINFDLQQ AGRTFEDEET TEEAAREEYR KLAERRVRLG LVLSEIGEKA GVEVTEEELQ RAVYDQVRRY PGQEKEIYDF LRRTPDAVAN LRAPIFEEKV VDHLLANINV TDKKVSKEEL TAEDEDAASE AKPAKKAAAK KKAAPKKKAE EGKSEEA

NT Sequence

atgacaagaa gtgaaggttt gaacatgcag gttaccgaaa cgctcaatga agggctgaag cgcgagatca aagtcgtggt tccggccggg gatcttgaag ccaagctcgc tgagcggctc gaaaccgcgc gcggccgcgc ccgcatcaac ggcttccgtc cgggcaaggt gccgacggct cacctgcgca agatgtacgg caagtccttc atggccgaga tcgtcaacga aatcctcaac gattcgtcgc gttccatcct tgccgaacgc aacgaaaagt cggcgaccca gcctgaagtc atcatgtcgg aagacgaaaa agaagccgag aaggttctcg acggcaaggc cgatttcgtt ttctcgctga actatgaagt gctgccggca atcgaggtca aggacttctc caagatcgcc gtgacccgtg aagtcgtcga catttccgat gaggaagtcg atgaacaggt caagcgcatt gcgtcgtcga cccgcacctt tgaaaccaag aagggcaagg ccgaaaacga agatcgcgtc acgatcgact atctgggcaa gctcgacggc gagccgtttg aaggcggtgc agacaatgac gcacagctcg ttctcggttc cggccagttc attccgggct ttgaagaaca gctcattggc ttgaaggctg gcgacgagaa ggtcatcacc gtaacgttcc cggctgaata cggcgctgcg catctggctg gcaaggaagc aaccttcgac atcaaggtga aggaagttgc caagccgaac gaactcgttc tcgatgacga aaccgcaaag aagctcggca ttgagtcgct tgagcgtctg cgtcaggttg tgcgcgaaca gatcgaaagc cagtacggcc agatcacccg ccagaaagtg aagcgtcaga ttctcgacgc ccttgatggt gattatcagt ttgaaacccc gcagaagctg gttgacgccg agttcaacaa catctggcag cagatcaact tcgacctgca gcaggctggc cgcacctttg aagacgagga aacgacggaa gaagctgctc gcgaagaata tcgcaagctt gcagaacgcc gcgttcgtct tggcctcgtt ctctccgaaa tcggcgagaa ggcaggcgtg gaagtgaccg aagaagaact gcagcgcgct gtttacgatc aggttcgccg ctatccgggt caggaaaagg aaatctacga cttcctgcgc cgtacgccgg atgccgtcgc caatctgcgc gcgccgatct ttgaagaaaa ggtcgtcgat catctgctgg ccaacatcaa tgtgaccgac aagaaggtct cgaaggaaga gctgacggct gaagacgaag atgcagcttc ggaagcaaag cctgccaaga aggcggctgc gaagaagaag gctgcgccga agaagaaggc cgaagaaggc aagtccgaag aggcttga

Details for BrmeA.17270.a.A1.GE34622

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	55kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	57

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacaa gaagtgaagg tttgaacatg caggttaccg aaacgctcaa tgaagggctg aagcgcgaga tcaaagtcgt ggttccggcc ggggatcttg aagccaagct cgctgagcgg ctcgaaaccg cgcgcggccg cgcccgcatc aacggcttcc gtccgggcaa ggtgccgacg gctcacctgc gcaagatgta cggcaagtcc ttcatggccg agatcgtcaa cgaaatcctc aacgattcgt cgcgttccat ccttgccgaa cgcaacgaaa agtcggcgac ccagcctgaa gtcatcatgt cggaagacga aaaagaagcc gagaaggttc tcgacggcaa ggccgatttc gttttctcgc tgaactatga agtgctgccg gcaatcgagg tcaaggactt ctccaagatc gccgtgaccc gtgaagtcgt cgacatttcc gatgaggaag tcgatgaaca ggtcaagcgc attgcgtcgt cgacccgcac ctttgaaacc aagaagggca aggccgaaaa cgaagatcgc gtcacgatcg actatctggg caagctcgac ggcgagccgt ttgaaggcgg tgcagacaat gacgcacagc tcgttctcgg ttccggccag ttcattccgg gctttgaaga acagctcatt ggcttgaagg ctggcgacga gaaggtcatc accgtaacgt tcccggctga atacggcgct gcgcatctgg ctggcaagga agcaaccttt cgacatcaag gtgaaggaag ttgccnagcc gaacgaactc gttctcgatg acgaaaccgc aagaagctcg gcattgagtc gcttgagcgt ctgcgtcngg ttgtgcgcga acngatcgaa ngccagtacg gccagatcac ccgncngaaa nngnnnnnnn nnannnnnnn ngnnnnnnnn nntnnnnnnt nnnnnnnnnn nnnnngagac cacaacggtt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnangnn tcntcnccat cnccntcana nnngtaccct ggaagctcag acccagggtc nngntnngat ggccgagaac aacgccgtcc ctacccaccn atcgntgntn gattttccgg aggtcnatct gcgcgcgatc gcgcngtcat gggagancna ncagttcaag cgggagctgc tcgccgatnc ncnggangcg ctcnagcgnt atttcgacnn nngcngcccg tggatcntga atnngaaggt cgccgaagtg ccgccggacg agtnncntta nggnngggac gncaagcggc agcgctngaa tctgcccgtc aacacgttgc gcgtcggcat tccgacgccg cngaagcacc tcgccgagga aggcatcgcn ctcgccgcat acancgacgc gggcccggcg tacctgttca cctgctgnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTRSEGLNM QVTETLNEGL KREIKVVVPA GDLEAKLAER LETARGRARI NGFRPGKVPT AHLRKMYGKS FMAEIVNEIL NDSSRSILAE RNEKSATQPE VIMSEDEKEA EKVLDGKADF VFSLNYEVLP AIEVKDFSKI AVTREVVDIS DEEVDEQVKR IASSTRTFET KKGKAENEDR VTIDYLGKLD GEPFEGGADN DAQLVLGSGQ FIPGFEEQLI GLKAGDEKVI TVTFPAEYGA AHLAGKEATF DIKVKEVAKP NELVLDDETA KKLGIESLER LRQVVREQIE SQYGQITRQK VKRQILDALD GDYQFETPQK LVDAEFNNIW QQINFDLQQA GRTFEDEETT EEAAREEYRK LAERRVRLGL VLSEIGEKAG VEVTEEELQR AVYDQVRRYP GQEKEIYDFL RRTPDAVANL RAPIFEEKVV DHLLANINVT DKKVSKEELT AEDEDAASEA KPAKKAAAKK KAAPKKKA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaagaagt gaaggtttga acatgcaggt taccgaaacg ctcaatgaag ggctgaagcg cgagatcaaa gtcgtggttc cggccgggga tcttgaagcc aagctcgctg agcggctcga aaccgcgcgc ggccgcgccc gcatcaacgg cttccgtccg ggcaaggtgc cgacggctca cctgcgcaag atgtacggca agtccttcat ggccgagatc gtcaacgaaa tcctcaacga ttcgtcgcgt tccatccttg ccgaacgcaa cgaaaagtcg gcgacccagc ctgaagtcat catgtcggaa gacgaaaaag aagccgagaa ggttctcgac ggcaaggccg atttcgtttt ctcgctgaac tatgaagtgc tgccggcaat cgaggtcaag gacttctcca agatcgccgt gacccgtgaa gtcgtcgaca tttccgatga ggaagtcgat gaacaggtca agcgcattgc gtcgtcgacc cgcacctttg aaaccaagaa gggcaaggcc gaaaacgaag atcgcgtcac gatcgactat ctgggcaagc tcgacggcga gccgtttgaa ggcggtgcag acaatgacgc acagctcgtt ctcggttccg gccagttcat tccgggcttt gaagaacagc tcattggctt gaaggctggc gacgagaagg tcatcaccgt aacgttcccg gctgaatacg gcgctgcgca tctggctggc aaggaagcaa ccttcgacat caaggtgaag gaagttgcca agccgaacga actcgttctc gatgacgaaa ccgcaaagaa gctcggcatt gagtcgcttg agcgtctgcg tcaggttgtg cgcgaacaga tcgaaagcca gtacggccag atcacccgcc agaaagtgaa gcgtcagatt ctcgacgccc ttgatggtga ttatcagttt gaaaccccgc agaagctggt tgacgccgag ttcaacaaca tctggcagca gatcaacttc gacctgcagc aggctggccg cacctttgaa gacgaggaaa cgacggaaga agctgctcgc gaagaatatc gcaagcttgc agaacgccgc gttcgtcttg gcctcgttct ctccgaaatc ggcgagaagg caggcgtgga agtgaccgaa gaagaactgc agcgcgctgt ttacgatcag gttcgccgct atccgggtca ggaaaaggaa atctacgact tcctgcgccg tacgccggat gccgtcgcca atctgcgcgc gccgatcttt gaagaaaagg tcgtcgatca tctgctggcc aacatcaatg tgaccgacaa gaaggtctcg aaggaagagc tgacggctga agacgaagat gcagcttcgg aagcaaagcc tgccaagaag gcggctgcga agaagaaggc tgcgccgaag aagaaggcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa