BrmeA.17127.a
Glucose-6-phosphate isomerase (GPI) (EC 5.3.1.9) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI)
| CENTER ID: | BrmeA.17127.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.17127.a.A1.GE33604 | full length | 1 | 549 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.17127.a.A1.PW34214 | full length | 1 | 549 |
Structures
4EM6
DEPOSITED: 4/11/2012
DETERMINATION: XRay
CLONE: BrmeA.17127.a.A1.GE33604
PROTEIN: BrmeA.17127.a.A1.PW34214
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126980 |
| PATRIC ID: | fig|224914.11.peg.1816 |
| RefSeq: | NP_540553.1 |
| UniProt: | Q8YF86 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARDATKLEA TVAKLKKHWA ESAPRDMRAA FSADPGRFGR YSLCLDDLLF DWSKCRVNDE TMALLKELAV AADVEGRRAA MFAGEHINNT EDRAVLHVAL RDTSSKEVLV DGHNVLPDVK HVLDRMAAFA DGIRSGALKG ATGRKITDIV NIGIGGSDLG PVMATLALAP YHDEPRAHFV SNIDGAHIAD TLSPLDPAST LIIVASKTFT TIETMTNAQT ARKWVADTLG EAAVGAHFAA VSTALDKVAA FGIPEDRVFG FWDWVGGRYS VWSAIGLPVM IAVGPDNFRK FLAGAHAMDV HFRDAPLEKN LPVMLGLIGY WHRAICGYGS RAIIPYDQRL SRLPAYLQQL DMESNGKSVT LDGKPVSGPT GPVVWGEPGT NGQHAFFQLL HQGTDTIPLE FIVAAKGHEP TLDHQHEMLM ANCLAQSEAL MKGRTLDEAR AQLQAKNLPA SQVERIAPHR VFSGNRPSLT LIHDMLDPYT LGRLIALYEH RVFVEAQIFG INAFDQWGVE LGKELATELL PVVSGKEGAS GRDASTQGLV AHLHARRKA
NT Sequence
atggcaagag acgcgacgaa gcttgaagca acggtcgcaa agctgaaaaa gcattgggcg gaatcggccc cgcgcgatat gcgcgcggca ttcagcgccg accccggccg gttcgggcga tattcgctct gcctggatga tcttctgttc gactggtcga agtgccgggt taacgacgag accatggcgc tgttgaaaga gctggccgtc gctgccgatg tggaagggcg ccgcgccgcc atgttcgcgg gcgagcatat caacaatacc gaagaccgcg ccgttctgca tgtggcgctg cgcgacacgt cctcgaagga agtgctggtt gacgggcata atgtactgcc ggatgtgaaa catgtgctcg accgcatggc cgcttttgcg gatggcatcc ggtccggcgc gttgaagggc gcgaccggcc ggaagattac cgatatcgtg aatatcggca ttggcggttc cgatctgggg cctgtcatgg cgacgctggc gcttgcgccc tatcatgatg agccacgcgc gcatttcgtt tccaatatcg atggcgcgca tattgccgac acgctttcgc cgcttgatcc ggcatcgacg cttatcattg tcgcgtcgaa gacttttacg accattgaaa ccatgacgaa cgcgcagacg gcgcgcaaat gggtggcaga caccctgggc gaggcggcgg tgggcgcgca ttttgctgcc gtttccactg cgctcgacaa ggttgcggcc ttcggcattc ctgaagatcg tgttttcggc ttctgggact gggttggcgg gcgttattcg gtctggtcgg ccatcggcct gccggtgatg atcgccgtcg gcccggataa tttccgcaag ttccttgcgg gcgcacacgc catggatgtg catttccgcg atgcgccact ggaaaagaac ctgcccgtca tgctcggcct catcggctat tggcatcgcg caatctgcgg ttatggcagc cgcgccatca ttccttatga ccagcgcctt tcgcgcctcc cggcctattt gcagcagctc gatatggaat cgaacggcaa gagcgtgacg ctggatggca agccggtttc cggcccaacc ggccccgttg tctggggtga gcccggcacc aatggccagc acgccttttt ccagcttctg catcagggaa ccgatacgat cccgctggaa ttcatcgtgg cggccaaggg ccacgagccg acactggatc accagcatga aatgctgatg gcaaactgcc ttgcccagtc cgaagccctg atgaaaggcc gcacgctgga cgaggcgcgt gcgcagttgc aggcaaagaa cctgcctgcg tcgcaagtgg agcgcattgc cccgcaccgc gtcttttccg gcaaccgccc atccctgacg ctcatccatg acatgctcga cccctatacg ctgggtcgcc tgatcgcgct ttacgaacat cgtgttttcg tcgaagcgca gattttcggc atcaatgcct tcgatcaatg gggcgtggaa ctgggtaagg aactggcgac ggaacttttg ccggttgtat cgggcaagga aggggcgagt ggtcgcgacg cttccacgca agggcttgtc gcgcatctgc atgcgcgaag gaaagcttga
Details for BrmeA.17127.a.A1.GE33604
| HARVESTED ON: | 10/31/2011 |
| SEQUENCED ON: | 11/4/2011 |
| EXPECTED MW: | 61kDa |
| OBSERVED MW: | 61kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 59 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARDATKLE ATVAKLKKHW AESAPRDMRA AFSADPGRFG RYSLCLDDLL FDWSKCRVND ETMALLKELA VAADVEGRRA AMFAGEHINN TEDRAVLHVA LRDTSSKEVL VDGHNVLPDV KHVLDRMAAF ADGIRSGALK GATGRKITDI VNIGIGGSDL GPVMATLALA PYHDEPRAHF VSXIDGAHIA DTLSPLDPAS TLIIVASKTF TTIETMTNAQ TARKWVADTL GEAAVGAHFA AXSTALXXLX PSAXLXSXXX XGLGXXXXXX GRXXXXXMIX XXRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttntttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggca agagacgcga cgaagcttga agcaacggtc gcaaagctga aaaagcattg ggcggaatcg gccccgcgcg atatgcgcgc ggcattcagc gccgaccccg gccggttcgg gcgatattcg ctctgcctgg atgatcttct gttcgactgg tcgaagtgcc gggttaacga cgagaccatg gcgctgttga aagagctggc cgtcgctgcc gatgtggaag ggcgccgcgc cgccatgttc gcgggcgagc atatcaacaa taccgaagac cgcgccgttc tgcatgtggc gctgcgcgac acgtcctcga aggaagtgct ggttgacggg cataatgtac tgccggatgt gaaacatgtg ctcgaccgca tggccgcttt tgcggatggc atccggtccg gcgcgttgaa gggcgcgacc ggccggaaga ttaccgatat cgtgaatatc ggcattggcg gttccgatct ggggcctgtc atggcgacgc tggcgcttgc gccctatcat gatgagccac gcgcgcattt cgtttccnat atcgatggcg cgcatattgc cgacacgctt tcgccgcttg atccggcatc gacgcttatc attgtcgcgt cgaagacttt tacgaccatt gaaaccatga cgaacgcgca gacngcgcgc aaatgggtgg cagacaccct gggcgaggcg gcggtgggcg cgcattttgc tgccnnttcc actgcgctcg anangttgcn nccttcngca ttnctgannt cgngtttnnn tnngggactg ggntngcngg nntntnnnnc tggncggnnt cngnntnnnn nnatgatcnn nnnngnccgg annnnnnnnn nnnnnttnnc nnnnnnggnn cnnnnnnnnn nnnnnnnnnn nnnnnccncn nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARDATKLE ATVAKLKKHW AESAPRDMRA AFSADPGRFG RYSLCLDDLL FDWSKCRVND ETMALLKELA VAADVEGRRA AMFAGEHINN TEDRAVLHVA LRDTSSKEVL VDGHNVLPDV KHVLDRMAAF ADGIRSGALK GATGRKITDI VNIGIGGSDL GPVMATLALA PYHDEPRAHF VSNIDGAHIA DTLSPLDPAS TLIIVASKTF TTIETMTNAQ TARKWVADTL GEAAVGAHFA AVSTALDKVA AFGIPEDRVF GFWDWVGGRY SVWSAIGLPV MIAVGPDNFR KFLAGAHAMD VHFRDAPLEK NLPVMLGLIG YWHRAICGYG SRAIIPYDQR LSRLPAYLQQ LDMESNGKSV TLDGKPVSGP TGPVVWGEPG TNGQHAFFQL LHQGTDTIPL EFIVAAKGHE PTLDHQHEML MANCLAQSEA LMKGRTLDEA RAQLQAKNLP ASQVERIAPH RVFSGNRPSL TLIHDMLDPY TLGRLIALYE HRVFVEAQIF GINAFDQWGV ELGKELATEL LPVVSGKEGA SGRDASTQGL VAHLHARRKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaagagac gcgacgaagc ttgaagcaac ggtcgcaaag ctgaaaaagc attgggcgga atcggccccg cgcgatatgc gcgcggcatt cagcgccgac cccggccggt tcgggcgata ttcgctctgc ctggatgatc ttctgttcga ctggtcgaag tgccgggtta acgacgagac catggcgctg ttgaaagagc tggccgtcgc tgccgatgtg gaagggcgcc gcgccgccat gttcgcgggc gagcatatca acaataccga agaccgcgcc gttctgcatg tggcgctgcg cgacacgtcc tcgaaggaag tgctggttga cgggcataat gtactgccgg atgtgaaaca tgtgctcgac cgcatggccg cttttgcgga tggcatccgg tccggcgcgt tgaagggcgc gaccggccgg aagattaccg atatcgtgaa tatcggcatt ggcggttccg atctggggcc tgtcatggcg acgctggcgc ttgcgcccta tcatgatgag ccacgcgcgc atttcgtttc caatatcgat ggcgcgcata ttgccgacac gctttcgccg cttgatccgg catcgacgct tatcattgtc gcgtcgaaga cttttacgac cattgaaacc atgacgaacg cgcagacggc gcgcaaatgg gtggcagaca ccctgggcga ggcggcggtg ggcgcgcatt ttgctgccgt ttccactgcg ctcgacaagg ttgcggcctt cggcattcct gaagatcgtg ttttcggctt ctgggactgg gttggcgggc gttattcggt ctggtcggcc atcggcctgc cggtgatgat cgccgtcggc ccggataatt tccgcaagtt ccttgcgggc gcacacgcca tggatgtgca tttccgcgat gcgccactgg aaaagaacct gcccgtcatg ctcggcctca tcggctattg gcatcgcgca atctgcggtt atggcagccg cgccatcatt ccttatgacc agcgcctttc gcgcctcccg gcctatttgc agcagctcga tatggaatcg aacggcaaga gcgtgacgct ggatggcaag ccggtttccg gcccaaccgg ccccgttgtc tggggtgagc ccggcaccaa tggccagcac gcctttttcc agcttctgca tcagggaacc gatacgatcc cgctggaatt catcgtggcg gccaagggcc acgagccgac actggatcac cagcatgaaa tgctgatggc aaactgcctt gcccagtccg aagccctgat gaaaggccgc acgctggacg aggcgcgtgc gcagttgcag gcaaagaacc tgcctgcgtc gcaagtggag cgcattgccc cgcaccgcgt cttttccggc aaccgcccat ccctgacgct catccatgac atgctcgacc cctatacgct gggtcgcctg atcgcgcttt acgaacatcg tgttttcgtc gaagcgcaga ttttcggcat caatgccttc gatcaatggg gcgtggaact gggtaaggaa ctggcgacgg aacttttgcc ggttgtatcg ggcaaggaag gggcgagtgg tcgcgacgct tccacgcaag ggcttgtcgc gcatctgcat gcgcgaagga aagcttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrmeA.17127.a.A1.PW34214
| PURIFICATION DATe: | 12/16/2011 |
| CONCENTRATION: | 39.92mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 59 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARDATKLE ATVAKLKKHW AESAPRDMRA AFSADPGRFG RYSLCLDDLL FDWSKCRVND ETMALLKELA VAADVEGRRA AMFAGEHINN TEDRAVLHVA LRDTSSKEVL VDGHNVLPDV KHVLDRMAAF ADGIRSGALK GATGRKITDI VNIGIGGSDL GPVMATLALA PYHDEPRAHF VSXIDGAHIA DTLSPLDPAS TLIIVASKTF TTIETMTNAQ TARKWVADTL GEAAVGAHFA AXSTALXXLX PSAXLXSXXX XGLGXXXXXX GRXXXXXMIX XXRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttntttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggca agagacgcga cgaagcttga agcaacggtc gcaaagctga aaaagcattg ggcggaatcg gccccgcgcg atatgcgcgc ggcattcagc gccgaccccg gccggttcgg gcgatattcg ctctgcctgg atgatcttct gttcgactgg tcgaagtgcc gggttaacga cgagaccatg gcgctgttga aagagctggc cgtcgctgcc gatgtggaag ggcgccgcgc cgccatgttc gcgggcgagc atatcaacaa taccgaagac cgcgccgttc tgcatgtggc gctgcgcgac acgtcctcga aggaagtgct ggttgacggg cataatgtac tgccggatgt gaaacatgtg ctcgaccgca tggccgcttt tgcggatggc atccggtccg gcgcgttgaa gggcgcgacc ggccggaaga ttaccgatat cgtgaatatc ggcattggcg gttccgatct ggggcctgtc atggcgacgc tggcgcttgc gccctatcat gatgagccac gcgcgcattt cgtttccnat atcgatggcg cgcatattgc cgacacgctt tcgccgcttg atccggcatc gacgcttatc attgtcgcgt cgaagacttt tacgaccatt gaaaccatga cgaacgcgca gacngcgcgc aaatgggtgg cagacaccct gggcgaggcg gcggtgggcg cgcattttgc tgccnnttcc actgcgctcg anangttgcn nccttcngca ttnctgannt cgngtttnnn tnngggactg ggntngcngg nntntnnnnc tggncggnnt cngnntnnnn nnatgatcnn nnnngnccgg annnnnnnnn nnnnnttnnc nnnnnnggnn cnnnnnnnnn nnnnnnnnnn nnnnnccncn nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARDATKLE ATVAKLKKHW AESAPRDMRA AFSADPGRFG RYSLCLDDLL FDWSKCRVND ETMALLKELA VAADVEGRRA AMFAGEHINN TEDRAVLHVA LRDTSSKEVL VDGHNVLPDV KHVLDRMAAF ADGIRSGALK GATGRKITDI VNIGIGGSDL GPVMATLALA PYHDEPRAHF VSNIDGAHIA DTLSPLDPAS TLIIVASKTF TTIETMTNAQ TARKWVADTL GEAAVGAHFA AVSTALDKVA AFGIPEDRVF GFWDWVGGRY SVWSAIGLPV MIAVGPDNFR KFLAGAHAMD VHFRDAPLEK NLPVMLGLIG YWHRAICGYG SRAIIPYDQR LSRLPAYLQQ LDMESNGKSV TLDGKPVSGP TGPVVWGEPG TNGQHAFFQL LHQGTDTIPL EFIVAAKGHE PTLDHQHEML MANCLAQSEA LMKGRTLDEA RAQLQAKNLP ASQVERIAPH RVFSGNRPSL TLIHDMLDPY TLGRLIALYE HRVFVEAQIF GINAFDQWGV ELGKELATEL LPVVSGKEGA SGRDASTQGL VAHLHARRKA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAAGAGAC GCGACGAAGC TTGAAGCAAC GGTCGCAAAG CTGAAAAAGC ATTGGGCGGA ATCGGCCCCG CGCGATATGC GCGCGGCATT CAGCGCCGAC CCCGGCCGGT TCGGGCGATA TTCGCTCTGC CTGGATGATC TTCTGTTCGA CTGGTCGAAG TGCCGGGTTA ACGACGAGAC CATGGCGCTG TTGAAAGAGC TGGCCGTCGC TGCCGATGTG GAAGGGCGCC GCGCCGCCAT GTTCGCGGGC GAGCATATCA ACAATACCGA AGACCGCGCC GTTCTGCATG TGGCGCTGCG CGACACGTCC TCGAAGGAAG TGCTGGTTGA CGGGCATAAT GTACTGCCGG ATGTGAAACA TGTGCTCGAC CGCATGGCCG CTTTTGCGGA TGGCATCCGG TCCGGCGCGT TGAAGGGCGC GACCGGCCGG AAGATTACCG ATATCGTGAA TATCGGCATT GGCGGTTCCG ATCTGGGGCC TGTCATGGCG ACGCTGGCGC TTGCGCCCTA TCATGATGAG CCACGCGCGC ATTTCGTTTC CAATATCGAT GGCGCGCATA TTGCCGACAC GCTTTCGCCG CTTGATCCGG CATCGACGCT TATCATTGTC GCGTCGAAGA CTTTTACGAC CATTGAAACC ATGACGAACG CGCAGACGGC GCGCAAATGG GTGGCAGACA CCCTGGGCGA GGCGGCGGTG GGCGCGCATT TTGCTGCCGT TTCCACTGCG CTCGACAAGG TTGCGGCCTT CGGCATTCCT GAAGATCGTG TTTTCGGCTT CTGGGACTGG GTTGGCGGGC GTTATTCGGT CTGGTCGGCC ATCGGCCTGC CGGTGATGAT CGCCGTCGGC CCGGATAATT TCCGCAAGTT CCTTGCGGGC GCACACGCCA TGGATGTGCA TTTCCGCGAT GCGCCACTGG AAAAGAACCT GCCCGTCATG CTCGGCCTCA TCGGCTATTG GCATCGCGCA ATCTGCGGTT ATGGCAGCCG CGCCATCATT CCTTATGACC AGCGCCTTTC GCGCCTCCCG GCCTATTTGC AGCAGCTCGA TATGGAATCG AACGGCAAGA GCGTGACGCT GGATGGCAAG CCGGTTTCCG GCCCAACCGG CCCCGTTGTC TGGGGTGAGC CCGGCACCAA TGGCCAGCAC GCCTTTTTCC AGCTTCTGCA TCAGGGAACC GATACGATCC CGCTGGAATT CATCGTGGCG GCCAAGGGCC ACGAGCCGAC ACTGGATCAC CAGCATGAAA TGCTGATGGC AAACTGCCTT GCCCAGTCCG AAGCCCTGAT GAAAGGCCGC ACGCTGGACG AGGCGCGTGC GCAGTTGCAG GCAAAGAACC TGCCTGCGTC GCAAGTGGAG CGCATTGCCC CGCACCGCGT CTTTTCCGGC AACCGCCCAT CCCTGACGCT CATCCATGAC ATGCTCGACC CCTATACGCT GGGTCGCCTG ATCGCGCTTT ACGAACATCG TGTTTTCGTC GAAGCGCAGA TTTTCGGCAT CAATGCCTTC GATCAATGGG GCGTGGAACT GGGTAAGGAA CTGGCGACGG AACTTTTGCC GGTTGTATCG GGCAAGGAAG GGGCGAGTGG TCGCGACGCT TCCACGCAAG GGCTTGTCGC GCATCTGCAT GCGCGAAGGA AAGCTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA