BrmeA.10014.n

Oligopeptide transport ATP-binding protein OppD (TC 3.A.1.5.1)

CENTER ID: BrmeA.10014.n
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.n.B1.GE36699 Full length( BrmeA.10014.n ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_3206
BV-BRC: fig|224914.11.peg.3206
RefSeq: NP_541841.1
UniProt: Q8YBN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRQPILDIK GLRTVFRTRA REIVAVNDVD IVVNPGETVA LVGESGSGKS VTSLSIMRLL ARKVGFIDAG SIILRGKSGQ TVDLAAIDEE AMRRIRGNDI GMVFQEPMTS LNPVYTIGDQ IGEPLRVHRG TSRREALEAA VELLDRVGIP DARRRAGQYP HELSGGMRQR ATIAMALICN PTLLIADEPT TALDVTIQAQ ILDLMQKLQS ESGMGMLFVT HNLGVVAEIA QRVVVMYAGR IVESGPVKEV FRNPRHPYTM GLLRSMPRLG DATEMKRRGE KLNTIPGMVP GLANLPSGCA FAPRCSFAVE ACHAAVPPLA SVNKHHGSRC IRWQEIAA
NT Sequence
ATGAGCCGGC AACCGATCCT CGATATCAAG GGACTGCGCA CCGTCTTCCG CACCCGCGCG CGCGAGATCG TCGCGGTCAA TGATGTGGAC ATTGTCGTCA ATCCCGGCGA GACCGTGGCT CTGGTCGGCG AATCCGGCTC AGGCAAATCG GTAACGAGCC TTTCGATCAT GCGGCTTCTT GCCCGAAAGG TGGGCTTCAT CGATGCGGGC TCCATTATCC TGCGCGGCAA GAGCGGGCAG ACCGTGGACC TTGCCGCCAT TGATGAGGAA GCCATGCGCC GCATTCGCGG CAACGATATC GGCATGGTTT TCCAGGAACC CATGACGAGC CTCAATCCGG TCTATACGAT CGGCGACCAG ATCGGCGAAC CCTTGCGGGT TCACCGGGGC ACAAGCCGCC GCGAGGCGCT TGAAGCGGCA GTGGAACTTC TCGACCGGGT GGGCATTCCC GATGCCCGCC GCCGTGCCGG GCAATATCCG CATGAATTGT CGGGCGGCAT GCGCCAGCGC GCAACGATAG CCATGGCGCT GATATGCAAT CCGACGCTCT TGATCGCCGA TGAACCGACG ACGGCATTGG ACGTTACCAT TCAGGCCCAG ATTCTGGACC TCATGCAAAA GCTTCAAAGC GAAAGCGGCA TGGGCATGTT GTTCGTCACC CATAATCTGG GCGTTGTCGC GGAAATCGCC CAGCGGGTCG TGGTGATGTA TGCCGGGCGG ATTGTCGAGA GCGGCCCGGT GAAGGAAGTG TTCCGCAATC CGCGCCATCC CTACACGATG GGGCTGCTGC GTTCCATGCC GCGGCTTGGC GATGCGACGG AAATGAAAAG GCGCGGCGAA AAACTGAACA CTATTCCCGG CATGGTGCCG GGGCTTGCCA ATCTGCCTTC AGGCTGCGCT TTCGCACCGC GCTGTTCCTT CGCTGTCGAG GCGTGCCATG CGGCCGTTCC GCCGCTTGCA TCCGTCAACA AACACCATGG CAGCCGCTGC ATTCGCTGGC AGGAGATCGC CGCA
Details for BrmeA.10014.n.B1.GE36699
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMS RQPILDIKGL RTVFRTRARE IVAVNDVDIV VNPGETVALV GESGSGKSVT SLSIMRLLAR KVGFIDAGSI ILRGKSGQTV DLAAIDEEAM RRIRGNDIGM VFQEPMTSLN PVYTIGDQIG EPLRVHRGTS RREALEAAVE LLDRVGIPDA RRRAGQYPHE LSGGMRQRAT IAMALICNPT LLIADEPTTA LDVTIQAQIL DLMQKLQSES GMGMLFVTHN LGVVAEIAQR VVXMYAGRIV ESGPVKEVFR NPRHPYTMGL LRSMPRLXMX XXEKXRRKTE HYSRXGAGLX XLPSXALSXX AVPSXXXXXX
Validated NT Sequence
tannnatttt nnttacttta agaaggagan ataccatggc tcaccaccac caccaccata tgagccggca accgatcctc gatatcaagg gactgcgcac cgtcttccgc acccgcgcgc gcgagatcgt cgcggtcaat gatgtggaca ttgtcgtcaa tcccggcgag accgtggctc tggtcggcga atccggctca ggcaaatcgg taacgagcct ttcgatcatg cggcttcttg cccgaaaggt gggcttcatc gatgcgggct ccattatcct gcgcggcaag agcgggcaga ccgtggacct tgccgccatt gatgaggaag ccatgcgccg cattcgcggc aacgatatcg gcatggtttt ccaggaaccc atgacgagcc tcaatccggt ctatacgatc ggcgaccaga tcggcgaacc cttgcgggtt caccggggca caagccgccg cgaggcgctt gaagcggcag tggaacttct cgaccgggtg ggcattcccg atgcccgccg ccgtgccggg caatatccgc atgaattgtc gggcggcatg cgccagcgcg caacgatagc catggcgctg atatgcaatc cgacgctctt gatcgccgat gaaccgacga cggcattgga cgttaccatt caggcccaga ttctggacct catgcaaaag cttcaaagcg aaagcggcat gggcatgttg ttcgtcaccc ataatctggg cgttgtcgcg gaaatcgccc agcgggtcgt gntgatgtat gccgggcgga ttgtcgagag cggcccggtg aaggaagtgt tccgcaatcc gcgccatccc tacacgatgg ggctgctgcg ttccatgccg cggcttnnna tgcnacngan ngaaaagnnn cggcgaaaaa ctgaacacta ttcccggnat ggtgcngggc ttgnnnatct gccttcnnnt gcnctttcnn ancncgcngt tccttcnnnn ncnagnngnc nngnngc
Expected Protein Sequence
MAHHHHHHMS RQPILDIKGL RTVFRTRARE IVAVNDVDIV VNPGETVALV GESGSGKSVT SLSIMRLLAR KVGFIDAGSI ILRGKSGQTV DLAAIDEEAM RRIRGNDIGM VFQEPMTSLN PVYTIGDQIG EPLRVHRGTS RREALEAAVE LLDRVGIPDA RRRAGQYPHE LSGGMRQRAT IAMALICNPT LLIADEPTTA LDVTIQAQIL DLMQKLQSES GMGMLFVTHN LGVVAEIAQR VVVMYAGRIV ESGPVKEVFR NPRHPYTMGL LRSMPRLGDA TEMKRRGEKL NTIPGMVPGL ANLPSGCAFA PRCSFAVEAC HAAVPPLASV NKHHGSRCIR WQEIAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc ggcaaccgat cctcgatatc aagggactgc gcaccgtctt ccgcacccgc gcgcgcgaga tcgtcgcggt caatgatgtg gacattgtcg tcaatcccgg cgagaccgtg gctctggtcg gcgaatccgg ctcaggcaaa tcggtaacga gcctttcgat catgcggctt cttgcccgaa aggtgggctt catcgatgcg ggctccatta tcctgcgcgg caagagcggg cagaccgtgg accttgccgc cattgatgag gaagccatgc gccgcattcg cggcaacgat atcggcatgg ttttccagga acccatgacg agcctcaatc cggtctatac gatcggcgac cagatcggcg aacccttgcg ggttcaccgg ggcacaagcc gccgcgaggc gcttgaagcg gcagtggaac ttctcgaccg ggtgggcatt cccgatgccc gccgccgtgc cgggcaatat ccgcatgaat tgtcgggcgg catgcgccag cgcgcaacga tagccatggc gctgatatgc aatccgacgc tcttgatcgc cgatgaaccg acgacggcat tggacgttac cattcaggcc cagattctgg acctcatgca aaagcttcaa agcgaaagcg gcatgggcat gttgttcgtc acccataatc tgggcgttgt cgcggaaatc gcccagcggg tcgtggtgat gtatgccggg cggattgtcg agagcggccc ggtgaaggaa gtgttccgca atccgcgcca tccctacacg atggggctgc tgcgttccat gccgcggctt ggcgatgcga cggaaatgaa aaggcgcggc gaaaaactga acactattcc cggcatggtg ccggggcttg ccaatctgcc ttcaggctgc gctttcgcac cgcgctgttc cttcgctgtc gaggcgtgcc atgcggccgt tccgccgctt gcatccgtca acaaacacca tggcagccgc tgcattcgct ggcaggagat cgccgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at