SSGCID: Target Page

BrmeA.10014.m

Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1)

CENTER ID:	BrmeA.10014.m
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.10014.m.B1.GE36698	Full length( BrmeA.10014.m )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_2951
BV-BRC:	fig\|224914.11.peg.2951
RefSeq:	NP_541606.1
UniProt:	Q8YCA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAASLEIRN FSSGYGPTRV IEDVSFKAEP GSRLAILGRN GVGKTSLFAT IAGQTRRYGG EILLDGRNIA TLPSAARALA GLGYVPQTRD IFPTLTVEEN LFVGLKNRPK DALEEAYAMF PRLKERRRNL GSQLSGGEQQ MLSTARSILG QPTVLLLDEP LEGLAPVICE ELMAAFSALA QKGDMTILLV EQRIQSALDF ADHVIILERG RIAWSGTAAE LADNPQTVEE LLGVGGLH

NT Sequence

ATGAGTGCCG CCTCGCTTGA AATCCGCAAT TTCTCCTCCG GCTACGGTCC CACGCGTGTT ATCGAGGATG TATCCTTCAA GGCCGAACCC GGCTCGCGAC TTGCCATCCT TGGCCGCAAC GGCGTTGGCA AGACCAGTCT TTTTGCCACT ATCGCTGGCC AGACCCGCCG CTATGGCGGT GAAATTCTGC TGGACGGGCG GAATATAGCT ACGCTTCCTT CCGCCGCGCG CGCCCTTGCC GGCCTTGGCT ATGTGCCGCA GACTCGCGAT ATTTTTCCCA CGCTCACCGT TGAGGAAAAC CTTTTTGTCG GCCTGAAGAA CCGGCCAAAA GATGCGCTGG AAGAAGCCTA TGCGATGTTT CCGCGCCTGA AGGAACGCAG GCGCAATCTC GGCTCGCAGC TTTCAGGCGG CGAACAGCAG ATGCTTTCCA CCGCGCGCAG CATTCTCGGC CAGCCGACCG TGCTTTTGCT GGACGAGCCG CTGGAGGGGC TGGCACCCGT CATCTGCGAG GAACTCATGG CCGCGTTTTC TGCCCTTGCG CAGAAAGGCG ACATGACGAT CCTGCTGGTT GAACAACGCA TCCAAAGCGC GCTCGACTTT GCCGATCACG TCATCATTCT GGAGCGTGGA CGCATCGCCT GGTCAGGCAC CGCGGCGGAA CTCGCCGACA ACCCGCAGAC CGTTGAGGAA CTGCTGGGGG TCGGTGGCCT GCAC

Details for BrmeA.10014.m.B1.GE36698

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMS AASLEIRNFS SGYGPTRVIE DVSFKAEPGS RLAILGRNGV GKTSLFATIA GQTRRYGGEI LLDGRNIATL PSAARALAGL GYVPQTRDIF PTLTVEENLF VGLKNRPKDA LEEAYAMFPR LKERRRNLGS QLSGGEQQML STARSILGQP TVLLLDEPLE GLAPVICEEL MAAFSALAQK GDMTILLVEQ RIQSALDFAD HVIILERGRI AWSGTAAELA DNPQTVEELL GVGGLH

Validated NT Sequence

annntttnnt tantttaaga aggaganata ccatggctca ccaccaccac caccatatga gtgccgcctc gcttgaaatc cgcaatttct cctccggcta cggtcccacg cgtgttatcg aggatgtatc cttcaaggcc gaacccggct cgcgacttgc catccttggc cgcaacggcg ttggcaagac cagtcttttt gccactatcg ctggccagac ccgccgctat ggcggtgaaa ttctgctgga cgggcggaat atagctacgc ttccttccgc cgcgcgcgcc cttgccggcc ttggctatgt gccgcagact cgcgatattt ttcccacgct caccgttgag gaaaaccttt ttgtcggcct gaagaaccgg ccaaaagatg cgctggaaga agcctatgcg atgtttccgc gcctgaagga acgcaggcgc aatctcggct cgcagctttc aggcggcgaa cagcagatgc tttccaccgc gcgcagcatt ctcggccagc cgaccgtgct tttgctggac gagccgctgg aggggctggc acccgtcatc tgcgaggaac tcatggccgc gttttctgcc cttgcgcaga aaggcgacat gacgatcctg ctggttgaac aacgcatcca aagcgcgctc gactttgccg atcacgtcat cattctggag cgtggacgca tcgcctggtc aggcaccgcg gcggaactcg ccgacaaccc gcagaccgtt gaggaactgc tgggggtcgg tggcctgcac taagtgagta aataggatcc ggctgctaac aanncccgaa agannnccnc cncnnnnnnn ncnncnncnn ncnnnnnnnn ncttnncnnt gnncnnnnan nngnncgccn nnnncnnnnn ctnnnnnnnn nnnnnnnnnn nctnnnaatn nncntnnnnn ncnnnnnnnn nnnnnnnntc nncnnnnnnn nc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg ccgcctcgct tgaaatccgc aatttctcct ccggctacgg tcccacgcgt gttatcgagg atgtatcctt caaggccgaa cccggctcgc gacttgccat ccttggccgc aacggcgttg gcaagaccag tctttttgcc actatcgctg gccagacccg ccgctatggc ggtgaaattc tgctggacgg gcggaatata gctacgcttc cttccgccgc gcgcgccctt gccggccttg gctatgtgcc gcagactcgc gatatttttc ccacgctcac cgttgaggaa aacctttttg tcggcctgaa gaaccggcca aaagatgcgc tggaagaagc ctatgcgatg tttccgcgcc tgaaggaacg caggcgcaat ctcggctcgc agctttcagg cggcgaacag cagatgcttt ccaccgcgcg cagcattctc ggccagccga ccgtgctttt gctggacgag ccgctggagg ggctggcacc cgtcatctgc gaggaactca tggccgcgtt ttctgccctt gcgcagaaag gcgacatgac gatcctgctg gttgaacaac gcatccaaag cgcgctcgac tttgccgatc acgtcatcat tctggagcgt ggacgcatcg cctggtcagg caccgcggcg gaactcgccg acaacccgca gaccgttgag gaactgctgg gggtcggtgg cctgcactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at