BrmeA.10014.l

Ferric iron ABC transporter, ATP-binding protein

CENTER ID: BrmeA.10014.l
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.l.B1.GE36697 Full length( BrmeA.10014.l ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2880
BV-BRC: fig|224914.11.peg.2880
RefSeq: NP_541545.1
UniProt: Q8YCG3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAIRPGSVT FENVTKKFGN FTALPNLSLT VEPGTLVTLL GLSGCGKTTT LRLLAGLEHP TSGRILIGGK DVTNLPANER DVSMVFQSYA LFPHMTSLEN VAYGLESSGF KKNEARERAE EGLKLVGLGG MGHRLPAELS GGQQQRVAVA RALVLEPQVL LLDEPLSNLD ARLRRRVRTE IRELQQRLGF TAVYVTHDQD EALAVSDTII VMKEGGIAQK GSPRDLYEAP ASAFIADFMG EANVVPCEVI SAENGEAVIR VAGLTHRVPA RNAQPGPAQL AIRPNAVTLQ PQAGGGFSGT VAHSAYLGDH IEYEIETEHG KLFIVDPAVE QSLPLQTDVS IQFKTRGLAI INQ
NT Sequence
ATGACCGCGA TCCGCCCCGG CTCCGTAACC TTTGAAAACG TCACCAAAAA ATTCGGCAAT TTCACCGCAT TGCCCAATCT GTCGCTGACG GTGGAACCCG GTACGCTGGT CACGCTGCTG GGGCTTTCCG GCTGCGGCAA GACCACGACG CTGCGCCTGC TGGCCGGTCT GGAACATCCG ACTTCGGGGC GCATTCTCAT CGGTGGCAAG GATGTGACCA ATCTGCCTGC CAATGAGCGT GACGTGTCGA TGGTCTTCCA GTCCTATGCG CTTTTTCCGC ATATGACCTC GCTTGAAAAC GTCGCCTATG GGCTTGAATC TTCCGGCTTC AAGAAGAACG AAGCGCGCGA GCGCGCGGAA GAGGGATTGA AGCTGGTGGG CCTCGGCGGG ATGGGGCATC GCTTGCCAGC CGAATTGTCT GGCGGGCAGC AGCAGCGCGT GGCAGTGGCC CGCGCGCTCG TGCTGGAGCC GCAGGTTCTG CTTCTGGATG AGCCGCTTTC CAACCTCGAT GCACGCCTTC GCCGCCGCGT GCGCACGGAA ATCCGTGAAT TGCAGCAGCG GCTTGGTTTC ACCGCCGTTT ATGTGACGCA TGACCAGGAC GAGGCGCTCG CCGTTTCGGA TACGATCATT GTTATGAAAG AGGGCGGAAT TGCCCAGAAG GGCAGCCCGC GCGATCTTTA CGAGGCTCCA GCTTCGGCCT TCATCGCCGA TTTCATGGGC GAGGCCAATG TCGTGCCCTG CGAGGTGATA AGCGCGGAAA ATGGCGAGGC TGTCATCCGC GTGGCTGGCC TTACCCATCG CGTGCCAGCA CGAAATGCCC AACCCGGACC GGCACAGCTT GCCATCCGGC CCAATGCCGT CACCTTGCAG CCGCAGGCGG GTGGCGGGTT TTCCGGCACG GTCGCACATT CTGCCTATCT CGGCGATCAT ATCGAATATG AGATAGAGAC CGAACACGGA AAGCTCTTCA TCGTCGATCC GGCCGTGGAA CAGTCCTTGC CGCTCCAGAC GGATGTTTCC ATCCAGTTCA AGACACGCGG CCTTGCCATC ATCAACCAA
Details for BrmeA.10014.l.B1.GE36697
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMT AIRPGSVTFE NVTKKFGNFT ALPNLSLTVE PGTLVTLLGL SGCGKTTTLR LLAGLEHPTS GRILIGGKDV TNLPANERDV SMVFQSYALF PHMTSLENVA YGLESSGFKK NEARERAEEG LKLVGLGGMG HRLPAELSGG QQQRVAVARA LVLEPQVLLL DEPLSNLDAR LRRRVRTEIR ELQQRLGFTA VYVTHDQDEA LAVSDTIIVM KEGGIAQKGS PRDLYXAPAS AFIAXFMGEA NVVPCEVISA ENGEAVIRVA GLTHRVXARN AQPGPAQLAI XXQCRHLAAX GGGFXXHGXT FXLSRXSYRX XXXXRXXKXX XRXSXXG
Validated NT Sequence
cnnnnnnnnt ttnnttanct ttaagaagga ganataccat ggctcaccac caccaccacc atatgaccgc gatccgcccc ggctccgtaa cctttgaaaa cgtcaccaaa aaattcggca atttcaccgc attgcccaat ctgtcgctga cggtggaacc cggtacgctg gtcacgctgc tggggctttc cggctgcggc aagaccacga cgctgcgcct gctggccggt ctggaacatc cgacttcggg gcgcattctc atcggtggca aggatgtgac caatctgcct gccaatgagc gtgacgtgtc gatggtcttc cagtcctatg cgctttttcc gcatatgacc tcgcttgaaa acgtcgccta tgggcttgaa tcttccggct tcaagaagaa cgaagcgcgc gagcgcgcgg aagagggatt gaagctggtg ggcctcggcg ggatggggca tcgcttgcca gccgaattgt ctggcgggca gcagcagcgc gtggcagtgg cccgcgcgct cgtgctggag ccgcaggttc tgcttctgga tgagccgctt tccaacctcg atgcacgcct tcgccgccgc gtgcgcacgg aaatccgtga attgcagcag cggcttggtt tcaccgccgt ttatgtgacg catgaccagg acgaggcgct cgccgtttcg gatacgatca ttgttatgaa agagggcgga attgcccaga agggcagccc gcgcgatctt tacnaggctc cagcttcggc cttcatcgcc ganttcatgg gcgaggccaa tgtcgtgccc tgcgaggtga taagcgcgga aaatggcgag gctgtcatcc gcgtggctgg ccttacccat cgcgtgncag cacgaaatgc ccaacccgga ccggcacagc ttgccatccn gncccaatgc cgtcaccttg cagccncggg tggcgggttt tncnggcacg gtcncacatt cngcctatct cgnnnatcat atcgannnnn anannnanac cgaannngga aanntnntcn ncgtnnatcn nnccnnggaa na
Expected Protein Sequence
MAHHHHHHMT AIRPGSVTFE NVTKKFGNFT ALPNLSLTVE PGTLVTLLGL SGCGKTTTLR LLAGLEHPTS GRILIGGKDV TNLPANERDV SMVFQSYALF PHMTSLENVA YGLESSGFKK NEARERAEEG LKLVGLGGMG HRLPAELSGG QQQRVAVARA LVLEPQVLLL DEPLSNLDAR LRRRVRTEIR ELQQRLGFTA VYVTHDQDEA LAVSDTIIVM KEGGIAQKGS PRDLYEAPAS AFIADFMGEA NVVPCEVISA ENGEAVIRVA GLTHRVPARN AQPGPAQLAI RPNAVTLQPQ AGGGFSGTVA HSAYLGDHIE YEIETEHGKL FIVDPAVEQS LPLQTDVSIQ FKTRGLAIIN Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cgatccgccc cggctccgta acctttgaaa acgtcaccaa aaaattcggc aatttcaccg cattgcccaa tctgtcgctg acggtggaac ccggtacgct ggtcacgctg ctggggcttt ccggctgcgg caagaccacg acgctgcgcc tgctggccgg tctggaacat ccgacttcgg ggcgcattct catcggtggc aaggatgtga ccaatctgcc tgccaatgag cgtgacgtgt cgatggtctt ccagtcctat gcgctttttc cgcatatgac ctcgcttgaa aacgtcgcct atgggcttga atcttccggc ttcaagaaga acgaagcgcg cgagcgcgcg gaagagggat tgaagctggt gggcctcggc gggatggggc atcgcttgcc agccgaattg tctggcgggc agcagcagcg cgtggcagtg gcccgcgcgc tcgtgctgga gccgcaggtt ctgcttctgg atgagccgct ttccaacctc gatgcacgcc ttcgccgccg cgtgcgcacg gaaatccgtg aattgcagca gcggcttggt ttcaccgccg tttatgtgac gcatgaccag gacgaggcgc tcgccgtttc ggatacgatc attgttatga aagagggcgg aattgcccag aagggcagcc cgcgcgatct ttacgaggct ccagcttcgg ccttcatcgc cgatttcatg ggcgaggcca atgtcgtgcc ctgcgaggtg ataagcgcgg aaaatggcga ggctgtcatc cgcgtggctg gccttaccca tcgcgtgcca gcacgaaatg cccaacccgg accggcacag cttgccatcc ggcccaatgc cgtcaccttg cagccgcagg cgggtggcgg gttttccggc acggtcgcac attctgccta tctcggcgat catatcgaat atgagataga gaccgaacac ggaaagctct tcatcgtcga tccggccgtg gaacagtcct tgccgctcca gacggatgtt tccatccagt tcaagacacg cggccttgcc atcatcaacc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat