BrmeA.10014.k

N-Acetyl-D-glucosamine ABC transport system ATP-binding protein

CENTER ID: BrmeA.10014.k
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.k.B1.GE36696 Full length( BrmeA.10014.k ) 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2857
BV-BRC: fig|224914.11.peg.2857
RefSeq: NP_541522.1
UniProt: Q8YCI4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEVSLRGIS KTFGQLTVLD RIDLEIHSGE FLVLVGPSGC GKSTLLRMVA GLEPISGGDL VIGGERANEL PPQKRNIAMV FQSYALFPHM TARENIGFGP RIRGEKAAET AAKVDHAASI LNLHSYLDRY PRQLSGGQRQ RVAMGRAIVR EPSVFLFDEP LSNLDAQLRV QMRTEIKALH QRLKSTVIYV THDQIEAMTM ADRIVVMNQG KIQQIGAPLD LYDRPANKFV AGFIGSPSMS FIPGTVADGF FCTGEGEKIA VSAAAKGARA AEAGIRPENF VIAREGAGLT LVVEVIEPTG PETHIYGRIA GEPVRAVFRE RIQLAPGEQV PVTAGSEHIH LFDKESGLPL
NT Sequence
ATGAAAGAGG TTTCGCTACG CGGAATTAGC AAGACTTTCG GACAGTTGAC TGTTCTCGAT CGGATTGATC TGGAGATCCA TAGCGGAGAG TTTCTCGTGC TTGTCGGCCC GTCCGGTTGC GGAAAATCCA CGCTTTTGCG CATGGTGGCG GGGCTGGAGC CGATTTCCGG CGGCGATCTG GTCATTGGCG GCGAGCGCGC CAACGAATTG CCGCCGCAAA AACGCAATAT TGCGATGGTG TTCCAGTCCT ATGCGCTGTT TCCACATATG ACCGCGCGCG AGAATATTGG TTTCGGCCCC CGTATTCGCG GCGAGAAAGC GGCGGAAACT GCGGCCAAGG TGGATCATGC GGCCTCCATT CTCAATCTCC ATTCCTATCT TGACCGATAT CCGCGCCAGC TTTCGGGCGG GCAGCGCCAG CGCGTGGCCA TGGGCCGCGC CATCGTGCGC GAGCCTTCGG TATTTCTCTT TGACGAGCCG CTTTCCAATC TCGATGCGCA ATTGCGCGTC CAGATGCGCA CCGAAATCAA GGCGCTGCAT CAGCGCCTGA AATCGACCGT GATCTATGTC ACGCATGACC AGATCGAGGC CATGACCATG GCCGACCGCA TCGTGGTGAT GAATCAGGGC AAGATCCAGC AGATAGGCGC GCCGCTCGAC CTCTATGATC GCCCAGCCAA TAAATTTGTG GCGGGCTTTA TCGGCTCGCC ATCGATGAGC TTCATTCCCG GCACGGTGGC CGATGGCTTT TTCTGTACGG GCGAGGGCGA AAAAATTGCC GTTTCGGCGG CCGCAAAGGG CGCACGCGCG GCGGAGGCGG GCATTCGCCC GGAAAATTTC GTTATCGCGC GCGAAGGCGC AGGCCTGACG CTTGTGGTGG AAGTGATCGA GCCGACCGGC CCGGAAACCC ATATCTATGG GCGCATTGCG GGAGAACCCG TCCGCGCCGT GTTCCGCGAG CGGATACAGC TTGCGCCGGG CGAGCAGGTT CCTGTAACGG CTGGGAGCGA ACATATTCAT CTCTTTGATA AGGAAAGCGG TTTGCCGCTT
Details for BrmeA.10014.k.B1.GE36696
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMK EVSLRGISKT FGQLTVLDRI DLEIHSGEFL VLVGPSGCGK STLLRMVAGL EPISGGDLVI GGERANELPP QKRNIAMVFQ SYALFPHMTA RENIGFGPRI RGEKAAETAA KVDHAASILN LHSYLDRYPR QLSGGQRQRV AMGRAIVREP SVFLFDEPLS NLDAQLRVQM RTEIKALHQR LKSTVIYVTH DQIEAMTMAD RIVVMNQGKI QQIGAPLDLY DRPANKFVAG FIGSPSMSFI PGTVADGFFC TGEGEKIAFR RPQRAHARRX XAFAXXNFVI AREGXX
Validated NT Sequence
ctannnnatt ttnnttanct ttaagaagga ganataccat ggctcaccac caccaccacc atatgaaaga ggtttcgcta cgcggaatta gcaagacttt cggacagttg actgttctcg atcggattga tctggagatc catagcggag agtttctcgt gcttgtcggc ccgtccggtt gcggaaaatc cacgcttttg cgcatggtgg cggggctgga gccgatttcc ggcggcgatc tggtcattgg cggcgagcgc gccaacgaat tgccgccgca aaaacgcaat attgcgatgg tgttccagtc ctatgcgctg tttccacata tgaccgcgcg cgagaatatt ggtttcggcc cccgtattcg cggcgagaaa gcggcggaaa ctgcggccaa ggtggatcat gcggcctcca ttctcaatct ccattcctat cttgaccgat atccgcgcca gctttcgggc gggcagcgcc agcgcgtggc catgggccgc gccatcgtgc gcgagccttc ggtatttctc tttgacgagc cgctttccaa tctcgatgcg caattgcgcg tccagatgcg caccgaaatc aaggcgctgc atcagcgcct gaaatcgacc gtgatctatg tcacgcatga ccagatcgag gccatgacca tggccgaccg catcgtggtg atgaatcagg gcaagatcca gcagataggc gcgccgctcg acctctatga tcgcccagcc aataaatttg tggcgggctt tatcggctcg ccatcgatga gcttcattcc cggcacggtg gccgatggct ttttctgtac gggcgagggc gaaaaaattg cgtttcggcg gccgcaaagg gcgcacgcgc ggcggnnnng ggcattcgcc cngnaaaatt tcgttatcgc gcgcgaaggn ncnnnntgan ncttgngagt gatcgagccn acngnccnna nnccatatct atggncncat tnnngnanan cnntcnnncn ngntcncnan nngatncngc ttnnnnnngn nnannngtnc nngnnang
Expected Protein Sequence
MAHHHHHHMK EVSLRGISKT FGQLTVLDRI DLEIHSGEFL VLVGPSGCGK STLLRMVAGL EPISGGDLVI GGERANELPP QKRNIAMVFQ SYALFPHMTA RENIGFGPRI RGEKAAETAA KVDHAASILN LHSYLDRYPR QLSGGQRQRV AMGRAIVREP SVFLFDEPLS NLDAQLRVQM RTEIKALHQR LKSTVIYVTH DQIEAMTMAD RIVVMNQGKI QQIGAPLDLY DRPANKFVAG FIGSPSMSFI PGTVADGFFC TGEGEKIAVS AAAKGARAAE AGIRPENFVI AREGAGLTLV VEVIEPTGPE THIYGRIAGE PVRAVFRERI QLAPGEQVPV TAGSEHIHLF DKESGLPL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aggtttcgct acgcggaatt agcaagactt tcggacagtt gactgttctc gatcggattg atctggagat ccatagcgga gagtttctcg tgcttgtcgg cccgtccggt tgcggaaaat ccacgctttt gcgcatggtg gcggggctgg agccgatttc cggcggcgat ctggtcattg gcggcgagcg cgccaacgaa ttgccgccgc aaaaacgcaa tattgcgatg gtgttccagt cctatgcgct gtttccacat atgaccgcgc gcgagaatat tggtttcggc ccccgtattc gcggcgagaa agcggcggaa actgcggcca aggtggatca tgcggcctcc attctcaatc tccattccta tcttgaccga tatccgcgcc agctttcggg cgggcagcgc cagcgcgtgg ccatgggccg cgccatcgtg cgcgagcctt cggtatttct ctttgacgag ccgctttcca atctcgatgc gcaattgcgc gtccagatgc gcaccgaaat caaggcgctg catcagcgcc tgaaatcgac cgtgatctat gtcacgcatg accagatcga ggccatgacc atggccgacc gcatcgtggt gatgaatcag ggcaagatcc agcagatagg cgcgccgctc gacctctatg atcgcccagc caataaattt gtggcgggct ttatcggctc gccatcgatg agcttcattc ccggcacggt ggccgatggc tttttctgta cgggcgaggg cgaaaaaatt gccgtttcgg cggccgcaaa gggcgcacgc gcggcggagg cgggcattcg cccggaaaat ttcgttatcg cgcgcgaagg cgcaggcctg acgcttgtgg tggaagtgat cgagccgacc ggcccggaaa cccatatcta tgggcgcatt gcgggagaac ccgtccgcgc cgtgttccgc gagcggatac agcttgcgcc gggcgagcag gttcctgtaa cggctgggag cgaacatatt catctctttg ataaggaaag cggtttgccg ctttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat