BrmeA.10014.j

ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component

CENTER ID: BrmeA.10014.j
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.j.B1.GE36695 Full length( BrmeA.10014.j ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2849
BV-BRC: fig|224914.11.peg.2849
RefSeq: NP_541515.1
UniProt: Q8YCJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMLSARNVCW AAGCAEILHD VSLDVVKGEF LGIIGPNGSG KTTLLSLLSG IRRPRSGTVL LGNAPIQKFN RREIARRLAL VEQQAETTER ITARQAVELG RTPYLGALSP WSAADDAIVG KALENVDMAH LAGRCWHTLS GGERQRLHIA RALAQEPQIL LLDEPTNHLD IGHQIGLLDL VSRQGLTVVA ALHDLNHAAM FCDRIAVMDT GQLVALGSPR EVLSAESIRA VFGVEVDVEY HGVRGCHIRY RLPDRPALEL RCLA
NT Sequence
ATGATGCTTT CGGCACGCAA TGTTTGCTGG GCTGCGGGGT GCGCGGAAAT CCTGCATGAT GTCTCGCTCG ATGTGGTGAA GGGCGAATTT CTCGGCATTA TCGGGCCGAA CGGTTCAGGC AAGACAACCC TGCTTTCGCT GCTTTCCGGC ATCAGGCGGC CTCGCTCGGG CACAGTGCTT CTGGGCAATG CTCCGATTCA GAAGTTTAAC CGCCGCGAGA TCGCCCGACG TCTGGCGCTG GTGGAACAGC AGGCTGAAAC CACCGAGCGC ATTACGGCGC GCCAAGCCGT GGAACTGGGC CGTACACCCT ATCTGGGGGC GCTTTCGCCC TGGTCGGCGG CTGACGATGC GATTGTTGGG AAGGCGCTTG AGAACGTCGA TATGGCGCAT CTGGCCGGGC GCTGCTGGCA TACGCTTTCG GGCGGCGAGC GCCAGCGGCT CCATATCGCC CGCGCACTTG CACAGGAGCC GCAAATCCTG CTTCTGGACG AACCAACCAA CCATCTTGAT ATCGGCCACC AGATCGGGCT TCTCGATCTC GTCAGCCGTC AGGGGCTGAC GGTGGTGGCG GCGCTGCACG ACCTCAACCA CGCGGCCATG TTCTGTGATC GCATCGCGGT TATGGACACG GGGCAGCTCG TGGCGCTTGG GTCCCCGCGT GAGGTGTTGA GTGCTGAGTC TATCCGCGCC GTGTTCGGCG TGGAGGTCGA TGTCGAATAT CACGGTGTTC GCGGCTGCCA TATCCGCTAT CGCTTACCAG ACAGGCCAGC CCTAGAACTG AGGTGTTTGG CA
Details for BrmeA.10014.j.B1.GE36695
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMM LSARNVCWAA GCAEILHDVS LDVVKGEFLG IIGPNGSGKT TLLSLLSGIR RPRSGTVLLG NAPIQKFNRR EIARRLALVE QQAETTERIT ARQAVELGRT PYLGALSPWS AADDAIVGKA LENVDMAHLA GRCWHTLSGG ERQRLHIARA LAQEPQILLL DEPTNHLDIG HQIGLLDLVS RQGLTVVAAL HDLNHAAMFC DRIAVMDTGQ LVALGSPREV LSAESIRAVF GVXVDVEYHG VRGCHIRYRL PDRPALELRC LA
Validated NT Sequence
ctannnnttt tnnttaactt taagaaggag anataccatg gctcaccacc accaccacca tatgatgctt tcggcacgca atgtttgctg ggctgcgggg tgcgcggaaa tcctgcatga tgtctcgctc gatgtggtga agggcgaatt tctcggcatt atcgggccga acggttcagg caagacaacc ctgctttcgc tgctttccgg catcaggcgg cctcgctcgg gcacagtgct tctgggcaat gctccgattc agaagtttaa ccgccgcgag atcgcccgac gtctggcgct ggtggaacag caggctgaaa ccaccgagcg cattacggcg cgccaagccg tggaactggg ccgtacaccc tatctggggg cgctttcgcc ctggtcggcg gctgacgatg cgattgttgg gaaggcgctt gagaacgtcg atatggcgca tctggccggg cgctgctggc atacgctttc gggcggcgag cgccagcggc tccatatcgc ccgcgcactt gcacaggagc cgcaaatcct gcttctggac gaaccaacca accatcttga tatcggccac cagatcgggc ttctcgatct cgtcagccgt caggggctga cggtggtggc ggcgctgcac gacctcaacc acgcggccat gttctgtgat cgcatcgcgg ttatggacac ggggcagctc gtggcgcttg ggtccccgcg tgaggtgttg agtgctgagt ctatccgcgc cgtgttcggc gtgnaggtcg atgtcgaata tcacggtgtt cgcggctgcc atatccgcta tcgcttacca gacaggccag ccctagaact gaggtgtttg gcataagtga gtaanatagn ancnnnnnnn nnnncccccn nnnnnnannn nnaaannnng nnnnntgnnn nnnnnnccgg gccnnatgnc nnannnacnt nntnnnnnnn nntncnnnna ncnnnnnnnn gcnnccnnnn nggnnannnn nnncnccnnn nnnnnnnnnn gncnnntccn cgnnnnnccn na
Expected Protein Sequence
MAHHHHHHMM LSARNVCWAA GCAEILHDVS LDVVKGEFLG IIGPNGSGKT TLLSLLSGIR RPRSGTVLLG NAPIQKFNRR EIARRLALVE QQAETTERIT ARQAVELGRT PYLGALSPWS AADDAIVGKA LENVDMAHLA GRCWHTLSGG ERQRLHIARA LAQEPQILLL DEPTNHLDIG HQIGLLDLVS RQGLTVVAAL HDLNHAAMFC DRIAVMDTGQ LVALGSPREV LSAESIRAVF GVEVDVEYHG VRGCHIRYRL PDRPALELRC LA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgc tttcggcacg caatgtttgc tgggctgcgg ggtgcgcgga aatcctgcat gatgtctcgc tcgatgtggt gaagggcgaa tttctcggca ttatcgggcc gaacggttca ggcaagacaa ccctgctttc gctgctttcc ggcatcaggc ggcctcgctc gggcacagtg cttctgggca atgctccgat tcagaagttt aaccgccgcg agatcgcccg acgtctggcg ctggtggaac agcaggctga aaccaccgag cgcattacgg cgcgccaagc cgtggaactg ggccgtacac cctatctggg ggcgctttcg ccctggtcgg cggctgacga tgcgattgtt gggaaggcgc ttgagaacgt cgatatggcg catctggccg ggcgctgctg gcatacgctt tcgggcggcg agcgccagcg gctccatatc gcccgcgcac ttgcacagga gccgcaaatc ctgcttctgg acgaaccaac caaccatctt gatatcggcc accagatcgg gcttctcgat ctcgtcagcc gtcaggggct gacggtggtg gcggcgctgc acgacctcaa ccacgcggcc atgttctgtg atcgcatcgc ggttatggac acggggcagc tcgtggcgct tgggtccccg cgtgaggtgt tgagtgctga gtctatccgc gccgtgttcg gcgtggaggt cgatgtcgaa tatcacggtg ttcgcggctg ccatatccgc tatcgcttac cagacaggcc agccctagaa ctgaggtgtt tggcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat