SSGCID: Target Page

BrmeA.10014.i

Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2)

CENTER ID:	BrmeA.10014.i
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.10014.i.B1.GE36694	Full length( BrmeA.10014.i )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_2580
BV-BRC:	fig\|224914.11.peg.2580
RefSeq:	NP_541266.1
UniProt:	Q8YD89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDMILQVRD VVREYQLARA SFFLKPASLR VLHGVSVEIE AGQSLGIVGE SGSGKSTLAR AVMGLERPQS GQILINGQDI YALDRSGLRE ARKGFQAIFQ DPYGSLDPRH TVKRIISEPI VSLERGTSAR ERDNRVAEVL EAVGLPKASA EKYPHEFSGG QRQRIAIARA LITRPALIVA DEPVSALDVS IQAQVLNLMM DLQEKFGLSY LFISHDLGVV RAITDRVAVI YRGNIVEEGP TAEVFDHPRH DYTRALVDAV PKPFSGRRKR ARRLNPMNSL PE

NT Sequence

ATGAGCGACA TGATCTTGCA GGTACGTGAT GTCGTGCGGG AGTATCAGCT TGCGCGCGCG TCGTTTTTCT TAAAGCCAGC TTCCTTGCGT GTTCTCCACG GTGTGAGCGT TGAAATCGAG GCGGGGCAGA GCCTTGGCAT CGTTGGGGAA AGCGGTTCTG GCAAATCCAC GCTGGCGCGT GCCGTCATGG GGCTGGAACG CCCGCAATCC GGTCAGATTC TCATCAATGG TCAGGATATT TATGCGCTTG ATCGTTCAGG CCTGCGCGAA GCGCGCAAGG GCTTTCAGGC GATTTTTCAG GATCCTTATG GCTCGCTCGA TCCGCGCCAT ACCGTCAAGC GTATCATTTC CGAGCCGATT GTTTCGCTGG AGCGCGGCAC GAGCGCCCGC GAGCGCGACA ACCGCGTGGC CGAAGTGCTG GAGGCCGTCG GCTTGCCAAA GGCTTCGGCG GAAAAATATC CGCATGAGTT TTCCGGCGGC CAGCGTCAGC GCATTGCCAT TGCACGCGCG CTGATTACCC GGCCCGCGCT GATCGTTGCC GATGAGCCGG TTTCGGCGCT GGATGTTTCC ATTCAGGCAC AGGTTTTGAA CCTGATGATG GATTTGCAGG AGAAATTTGG CCTCAGCTAT CTTTTCATCA GTCACGACCT TGGTGTCGTG CGCGCCATCA CCGACCGTGT CGCCGTGATC TATCGCGGCA ATATTGTTGA AGAGGGGCCG ACCGCAGAAG TATTCGATCA TCCCCGCCAT GATTATACGC GGGCGCTGGT CGATGCCGTG CCAAAGCCTT TTTCCGGACG CCGCAAGCGG GCGCGCCGCC TCAATCCCAT GAATAGCCTG CCAGAA

Details for BrmeA.10014.i.B1.GE36694

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMS DMILQVRDVV REYQLARASF FLKPASLRVL HGVSVEIEAG QSLGIVGESG SGKSTLARAV MGLERPQSGQ ILINGQDIYA LDRSGLREAR KGFQAIFQDP YGSLDPRHTV KRIISEPIVS LERGTSARER DNRVAEVLEA VGLPKASAEK YPHEFSGGQR QRIAIARALI TRPALIVADE PVSALDVSIQ AQVLNLMMDL QEKFGLSYLF ISHDLGVVRA ITDRVAVIYR GNIVEXGPXA EVFDHPRHDY TRALVDAVPK PFSGRRXGRA ASIP

Validated NT Sequence

anttnnnnnn ntttaagang gaganatacc atggctcacc accaccacca ccatatgagc gacatgatct tgcaggtacg tgatgtcgtg cgggagtatc agcttgcgcg cgcgtcgttt ttcttaaagc cagcttcctt gcgtgttctc cacggtgtga gcgttgaaat cgaggcgggg cagagccttg gcatcgttgg ggaaagcggt tctggcaaat ccacgctggc gcgtgccgtc atggggctgg aacgcccgca atccggtcag attctcatca atggtcagga tatttatgcg cttgatcgtt caggcctgcg cgaagcgcgc aagggctttc aggcgatttt tcaggatcct tatggctcgc tcgatccgcg ccataccgtc aagcgtatca tttccgagcc gattgtttcg ctggagcgcg gcacgagcgc ccgcgagcgc gacaaccgcg tggccgaagt gctggaggcc gtcggcttgc caaaggcttc ggcggaaaaa tatccgcatg agttttccgg cggccagcgt cagcgcattg ccattgcacg cgcgctgatt acccggcccg cgctgatcgt tgccgatgag ccggtttcgg cgctggatgt ttccattcag gcacaggttt tgaacctgat gatggatttg caggagaaat ttggcctcag ctatcttttc atcagtcacg accttggtgt cgtgcgcgcc atcaccgacc gtgtcgccgt gatctatcgc ggcaatattg ttgaagangg gccgancgca gaagtattcg atcatccccg ccatgattat acgcgggcgc tggtcgatgc cgtgccaaag cctttttccg gacgccgcnn cgggcgcgcc gcctcaatcc catgaatagc ctgccanaat aagtgagtaa nanaggatcc nnntgnnnnn ccccnannnn nnnnnnnnnc nnnnnnnnan cnnnnnnnan nnnnnnnnnn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acatgatctt gcaggtacgt gatgtcgtgc gggagtatca gcttgcgcgc gcgtcgtttt tcttaaagcc agcttccttg cgtgttctcc acggtgtgag cgttgaaatc gaggcggggc agagccttgg catcgttggg gaaagcggtt ctggcaaatc cacgctggcg cgtgccgtca tggggctgga acgcccgcaa tccggtcaga ttctcatcaa tggtcaggat atttatgcgc ttgatcgttc aggcctgcgc gaagcgcgca agggctttca ggcgattttt caggatcctt atggctcgct cgatccgcgc cataccgtca agcgtatcat ttccgagccg attgtttcgc tggagcgcgg cacgagcgcc cgcgagcgcg acaaccgcgt ggccgaagtg ctggaggccg tcggcttgcc aaaggcttcg gcggaaaaat atccgcatga gttttccggc ggccagcgtc agcgcattgc cattgcacgc gcgctgatta cccggcccgc gctgatcgtt gccgatgagc cggtttcggc gctggatgtt tccattcagg cacaggtttt gaacctgatg atggatttgc aggagaaatt tggcctcagc tatcttttca tcagtcacga ccttggtgtc gtgcgcgcca tcaccgaccg tgtcgccgtg atctatcgcg gcaatattgt tgaagagggg ccgaccgcag aagtattcga tcatccccgc catgattata cgcgggcgct ggtcgatgcc gtgccaaagc ctttttccgg acgccgcaag cgggcgcgcc gcctcaatcc catgaatagc ctgccagaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat