BrmeA.10014.h

Oligopeptide transport ATP-binding protein OppD (TC 3.A.1.5.1)

CENTER ID: BrmeA.10014.h
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.h.B1.GE36693 Full length( BrmeA.10014.h ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2492
BV-BRC: fig|224914.11.peg.2492
RefSeq: NP_541183.1
UniProt: Q8YDH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTISLKTAPL LEVSNLSVDF RTDGGWINAV DDVNFTLAPR ETLGLVGESG SGKSVTALSL LRLHDQRNSR LGGSVRYKGE DLFTLATSRL QQIRGHEIAM VFQDPIHTLN PVLTIGRQIE EGLRLHHGLQ GREARKRAIE LLDRVRIPDV ARRIDEYPHR MSGGQRQRVM IAIAIAGDPK ILIADEPTTA LDVTVQAQIM ELLRNLRDEL SMSVILISHD LGLVSEFADR AMVMYAGQPV ETGPINKIFD EPLHPYTEGL LSAIPDLDDD LDRLPTIPGS IPEPSRRPPG CRFAPRCTFA QASCVKPQPI MSLTGGRASR CPPRLPTEEC VL
NT Sequence
ATGACGATTT CCCTCAAGAC TGCTCCTCTG CTCGAAGTCT CCAATCTCAG CGTGGATTTT CGCACCGACG GCGGCTGGAT CAATGCCGTG GATGATGTAA ACTTCACGCT CGCGCCACGC GAGACGCTGG GCCTTGTGGG CGAAAGCGGA TCGGGGAAAA GCGTGACCGC TCTATCCCTG CTGCGCCTTC ACGACCAGCG CAACAGCCGG CTGGGCGGCT CTGTCCGCTA CAAGGGCGAA GACCTGTTCA CGCTTGCGAC CAGCCGCCTT CAGCAAATCC GCGGACATGA GATCGCCATG GTGTTTCAGG ACCCGATCCA TACATTGAAC CCGGTTCTCA CCATTGGCAG GCAGATCGAG GAAGGCCTGC GGCTGCACCA TGGCCTGCAA GGCCGTGAAG CGCGCAAACG CGCTATCGAA CTGCTTGACC GCGTCCGCAT TCCCGATGTC GCCCGCCGCA TTGATGAATA TCCTCACCGC ATGTCGGGCG GGCAGCGCCA ACGTGTAATG ATCGCTATTG CGATTGCCGG CGATCCGAAA ATCCTGATCG CCGACGAACC GACGACCGCG CTCGATGTCA CGGTTCAGGC GCAGATCATG GAGCTACTCC GCAATCTGCG CGATGAGCTT TCCATGTCCG TCATCCTGAT TTCCCATGAT CTTGGACTGG TATCGGAATT CGCGGATCGG GCAATGGTCA TGTATGCAGG CCAGCCGGTG GAAACGGGTC CTATCAACAA GATTTTCGAT GAACCGCTTC ACCCTTATAC GGAAGGACTT CTCTCTGCGA TTCCGGATCT GGATGACGAT CTAGACCGGC TGCCAACCAT TCCGGGTTCC ATCCCCGAAC CGTCGCGTCG CCCGCCTGGA TGCCGCTTCG CCCCGCGCTG CACCTTCGCA CAGGCAAGCT GCGTCAAACC CCAACCCATA ATGAGCCTGA CGGGTGGGCG GGCCAGCCGC TGTCCGCCGC GGCTGCCCAC CGAGGAGTGT GTCCTA
Details for BrmeA.10014.h.B1.GE36693
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMT ISLKTAPLLE VSNLSVDFRT DGGWINAVDD VNFTLAPRET LGLVGESGSG KSVTALSLLR LHDQRNSRLG GSVRYKGEDL FTLATSRLQQ IRGHEIAMVF QDPIHTLNPV LTIGRQIEEG LRLHHGLQGR EARKRAIELL DRVRIPDVAR RIDEYPHRMS GGQRQRVMIA IAIAGDPKIL IADEPTTALD VTVQAQIMEL LRNLRDELSM SVILISHDLG LVSEFADRAM VMYAGQPVET GPINKIFDEP LHPYTEGLLS AIPDLDDDLX RLPTIPGSIP EPSRRPPGXR FAPRCTFAQA SCVKPXXXMX LXGGXXXXXR XXXXXXXXXX XXXXX
Validated NT Sequence
ctnnnnnnnn ttnnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgacgat ttccctcaag actgctcctc tgctcgaagt ctccaatctc agcgtggatt ttcgcaccga cggcggctgg atcaatgccg tggatgatgt aaacttcacg ctcgcgccac gcgagacgct gggccttgtg ggcgaaagcg gatcggggaa aagcgtgacc gctctatccc tgctgcgcct tcacgaccag cgcaacagcc ggctgggcgg ctctgtccgc tacaagggcg aagacctgtt cacgcttgcg accagccgcc ttcagcaaat ccgcggacat gagatcgcca tggtgtttca ggacccgatc catacattga acccggttct caccattggc aggcagatcg aggaaggcct gcggctgcac catggcctgc aaggccgtga agcgcgcaaa cgcgctatcg aactgcttga ccgcgtccgc attcccgatg tcgcccgccg cattgatgaa tatcctcacc gcatgtcggg cgggcagcgc caacgtgtaa tgatcgctat tgcgattgcc ggcgatccga aaatcctgat cgccgacgaa ccgacgaccg cgctcgatgt cacggttcag gcgcagatca tggagctact ccgcaatctg cgcgatgagc tttccatgtc cgtcatcctg atttcccatg atcttggact ggtatcggaa ttcgcggatc gggcaatggt catgtatgca ggccagccgg tggaaacggg tcctatcaac aagattttcg atgaaccgct tcacccttat acggaaggac ttctctctgc gattccggat ctggatgacg atctanaccg gctgccaacc attccgggtt ccatccccga accgtcgcgt cgcccgcctg gnngccgctt cgccccgcgc tgcaccttcg cacaggcaag ctgcgtcaaa ccccnacnca tnatgancct gannggnggn cngncancng nnnnccgcnn nngntncncn ncnnnnannn nnncnnnnan nnnntannnn nnnnc
Expected Protein Sequence
MAHHHHHHMT ISLKTAPLLE VSNLSVDFRT DGGWINAVDD VNFTLAPRET LGLVGESGSG KSVTALSLLR LHDQRNSRLG GSVRYKGEDL FTLATSRLQQ IRGHEIAMVF QDPIHTLNPV LTIGRQIEEG LRLHHGLQGR EARKRAIELL DRVRIPDVAR RIDEYPHRMS GGQRQRVMIA IAIAGDPKIL IADEPTTALD VTVQAQIMEL LRNLRDELSM SVILISHDLG LVSEFADRAM VMYAGQPVET GPINKIFDEP LHPYTEGLLS AIPDLDDDLD RLPTIPGSIP EPSRRPPGCR FAPRCTFAQA SCVKPQPIMS LTGGRASRCP PRLPTEECVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga tttccctcaa gactgctcct ctgctcgaag tctccaatct cagcgtggat tttcgcaccg acggcggctg gatcaatgcc gtggatgatg taaacttcac gctcgcgcca cgcgagacgc tgggccttgt gggcgaaagc ggatcgggga aaagcgtgac cgctctatcc ctgctgcgcc ttcacgacca gcgcaacagc cggctgggcg gctctgtccg ctacaagggc gaagacctgt tcacgcttgc gaccagccgc cttcagcaaa tccgcggaca tgagatcgcc atggtgtttc aggacccgat ccatacattg aacccggttc tcaccattgg caggcagatc gaggaaggcc tgcggctgca ccatggcctg caaggccgtg aagcgcgcaa acgcgctatc gaactgcttg accgcgtccg cattcccgat gtcgcccgcc gcattgatga atatcctcac cgcatgtcgg gcgggcagcg ccaacgtgta atgatcgcta ttgcgattgc cggcgatccg aaaatcctga tcgccgacga accgacgacc gcgctcgatg tcacggttca ggcgcagatc atggagctac tccgcaatct gcgcgatgag ctttccatgt ccgtcatcct gatttcccat gatcttggac tggtatcgga attcgcggat cgggcaatgg tcatgtatgc aggccagccg gtggaaacgg gtcctatcaa caagattttc gatgaaccgc ttcaccctta tacggaagga cttctctctg cgattccgga tctggatgac gatctagacc ggctgccaac cattccgggt tccatccccg aaccgtcgcg tcgcccgcct ggatgccgct tcgccccgcg ctgcaccttc gcacaggcaa gctgcgtcaa accccaaccc ataatgagcc tgacgggtgg gcgggccagc cgctgtccgc cgcggctgcc caccgaggag tgtgtcctat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat