BrmeA.10014.d

Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1)

CENTER ID: BrmeA.10014.d
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.10014.d.B1.GE36689 Full length( BrmeA.10014.d ) 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_0437
BV-BRC: fig|224914.11.peg.437
RefSeq: NP_539308.1
RefSeq: WP_004684117.1
UniProt: Q8YIQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDGRLQLRT GSVHALCGGN GAGKSTFLKI LMGIHARDGG HIRRRGKEVT YSSPAEALQA GIAIIEQELS PVPHMTVAEN IYLGREPLRR FGGIDFKTMN LNAQKLLDQL EFKIRANQFM MNLSVAQVQL VEIAKALSHD AEVIFMDEPT SAIGEKEAQQ LFAAIERLKA SGKGIVYVSH RLSEIFQIAD SYTVFRDGSY IDSGDLGGIT RAELIHMIVG RELGEEYIKT NAPTEIPGVE VSGLSAPGKI TDISFTAHKG EIFGIYGLMG SGRSEIFNCL FGLEKASQGT IRLEGEPVTV RRPADAMALG LAFVTEDRKL TGLNLGDSVR NNICLASLPE MSPHFSMNRR AESAASREMI ERFRIKVAHD TMAVSGLSGG NQQKVVLGKW FLRKPRVLLL DEPTRGVDVG AKQEIYRIIC EFAAQGGTVV MVSSEIDEVL GMSDRIMVMR GGQSVGIYRR AETDAQSLVH LST
NT Sequence
TTGCGGGATG GGCGGCTGCA ATTGCGTACC GGCTCGGTAC ATGCGCTGTG CGGCGGCAAT GGCGCGGGCA AATCCACGTT TTTGAAAATC CTGATGGGCA TTCATGCCCG CGATGGTGGA CATATCCGCC GGCGTGGAAA AGAGGTTACG TATTCCAGCC CCGCCGAAGC GTTGCAGGCG GGTATTGCGA TCATCGAACA GGAGCTAAGC CCCGTTCCCC ATATGACCGT GGCGGAGAAT ATCTATTTGG GCAGGGAGCC GCTTCGCCGT TTCGGTGGCA TCGATTTCAA GACGATGAAC CTCAATGCCC AGAAGCTTTT GGACCAGCTT GAGTTCAAGA TTCGCGCCAA CCAGTTCATG ATGAACCTGT CTGTCGCGCA GGTGCAGCTT GTGGAAATAG CCAAGGCGCT CAGTCACGAC GCTGAAGTCA TTTTCATGGA CGAACCGACA TCGGCCATTG GTGAAAAAGA AGCACAGCAG CTTTTTGCAG CGATAGAGCG TTTGAAAGCG AGCGGAAAAG GCATTGTCTA TGTCTCCCAT CGCCTGTCGG AAATTTTTCA GATTGCCGAT AGCTATACCG TGTTTCGCGA CGGCAGTTAT ATCGATAGCG GCGATCTCGG TGGCATCACC CGTGCGGAAC TGATCCATAT GATCGTCGGG CGGGAACTGG GTGAGGAATA TATCAAGACG AATGCGCCTA CGGAAATACC GGGCGTCGAG GTTTCCGGCC TCAGCGCGCC CGGCAAGATA ACCGACATTT CCTTTACCGC GCATAAGGGC GAGATTTTTG GCATTTACGG GTTGATGGGG TCAGGCCGCT CGGAAATATT CAACTGTCTT TTCGGATTGG AGAAAGCCTC CCAAGGAACA ATTCGGCTTG AAGGCGAACC CGTCACCGTC CGGCGCCCCG CGGACGCCAT GGCTCTGGGG CTGGCTTTTG TGACGGAAGA TCGCAAGCTG ACCGGCCTCA ATCTCGGCGA CAGCGTTCGC AACAATATAT GCCTTGCCAG CCTTCCCGAG ATGAGTCCGC ATTTTTCAAT GAATCGGCGT GCGGAATCGG CCGCCAGCAG GGAGATGATC GAGCGTTTCA GGATCAAGGT TGCGCACGAC ACGATGGCCG TTTCGGGCCT TTCCGGCGGC AACCAGCAGA AGGTCGTGCT CGGAAAATGG TTCCTGCGAA AGCCACGCGT TCTGCTGCTC GATGAACCCA CTCGCGGTGT CGATGTGGGT GCAAAACAGG AAATTTATCG CATCATTTGC GAATTTGCCG CCCAAGGCGG GACCGTCGTC ATGGTTTCTT CCGAGATTGA TGAAGTCCTC GGCATGTCTG ACCGCATCAT GGTCATGCGT GGCGGACAAT CGGTCGGCAT CTACCGCAGG GCGGAAACGG ACGCGCAGTC ATTGGTGCAT CTTTCGACA
Details for BrmeA.10014.d.B1.GE36689
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 54kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 69
Validated AA Sequence
MAHHHHHHML RDGRLQLRTG SVHALCGGNG AGKSTFLKIL MGIHARDGGH IRRRGKEVTY SSPAEALQAG IAIIEQELSP VPHMTVAENI YLGREPLRRF GGIDFKTMNL NAQKLLDQLE FKIRANQFMM NLSVAQVQLV EIAKALSHDA EVIFMDEPTS AIGEKEAQQL FAAIERLKAS GKGIVYVSHR LSEIFQIADS YTVFRDGSYI DSGDLGGITR AELIHMIVGR ELGEEYIKTN APTEIPGVEV SGLSAPGKIT DISFTAHXGE XFGIYGLMGS GRSEIFNCLF GLEKASXGTI RLEGXPXTVR RPRXPWLWGW LLXXXSQAXX XXXRXXXXXX XX
Validated NT Sequence
tnnnnnnnnt tnnnnnnntt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgttgcggg atgggcggct gcaattgcgt accggctcgg tacatgcgct gtgcggcggc aatggcgcgg gcaaatccac gtttttgaaa atcctgatgg gcattcatgc ccgcgatggt ggacatatcc gccggcgtgg aaaagaggtt acgtattcca gccccgccga agcgttgcag gcgggtattg cgatcatcga acaggagcta agccccgttc cccatatgac cgtggcggag aatatctatt tgggcaggga gccgcttcgc cgtttcggtg gcatcgattt caagacgatg aacctcaatg cccagaagct tttggaccag cttgagttca agattcgcgc caaccagttc atgatgaacc tgtctgtcgc gcaggtgcag cttgtggaaa tagccaaggc gctcagtcac gacgctgaag tcattttcat ggacgaaccg acatcggcca ttggtgaaaa agaagcacag cagctttttg cagcgataga gcgtttgaaa gcgagcggaa aaggcattgt ctatgtctcc catcgcctgt cggaaatttt tcagattgcc gatagctata ccgtgtttcg cgacggcagt tatatcgata gcggcgatct cggtggcatc acccgtgcgg aactgatcca tatgatcgtc gggcgggaac tgggtgagga atatatcaag acgaatgcgc ctacggaaat accgggcgtc gaggtttccg gcctcagcgc gcccggcaag ataaccgaca tttcctttac cgcgcataan ggcgagantt ttggcattta cgggttgatg gggtcaggnc gctcggaaat attcaactgt cttttcggat tggagaaagc ctcccnagga acaattcggc ttgaaggnna accnntcacc gtccggcgcc cgcggncgcc atggctctgg ggctggcttt tgnnannnan atcgcaagct gancnnnnnc nntnncgnnn nnnnnnnngc annnnnnnnt gncnt
Expected Protein Sequence
MAHHHHHHMR DGRLQLRTGS VHALCGGNGA GKSTFLKILM GIHARDGGHI RRRGKEVTYS SPAEALQAGI AIIEQELSPV PHMTVAENIY LGREPLRRFG GIDFKTMNLN AQKLLDQLEF KIRANQFMMN LSVAQVQLVE IAKALSHDAE VIFMDEPTSA IGEKEAQQLF AAIERLKASG KGIVYVSHRL SEIFQIADSY TVFRDGSYID SGDLGGITRA ELIHMIVGRE LGEEYIKTNA PTEIPGVEVS GLSAPGKITD ISFTAHKGEI FGIYGLMGSG RSEIFNCLFG LEKASQGTIR LEGEPVTVRR PADAMALGLA FVTEDRKLTG LNLGDSVRNN ICLASLPEMS PHFSMNRRAE SAASREMIER FRIKVAHDTM AVSGLSGGNQ QKVVLGKWFL RKPRVLLLDE PTRGVDVGAK QEIYRIICEF AAQGGTVVMV SSEIDEVLGM SDRIMVMRGG QSVGIYRRAE TDAQSLVHLS T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcggg atgggcggct gcaattgcgt accggctcgg tacatgcgct gtgcggcggc aatggcgcgg gcaaatccac gtttttgaaa atcctgatgg gcattcatgc ccgcgatggt ggacatatcc gccggcgtgg aaaagaggtt acgtattcca gccccgccga agcgttgcag gcgggtattg cgatcatcga acaggagcta agccccgttc cccatatgac cgtggcggag aatatctatt tgggcaggga gccgcttcgc cgtttcggtg gcatcgattt caagacgatg aacctcaatg cccagaagct tttggaccag cttgagttca agattcgcgc caaccagttc atgatgaacc tgtctgtcgc gcaggtgcag cttgtggaaa tagccaaggc gctcagtcac gacgctgaag tcattttcat ggacgaaccg acatcggcca ttggtgaaaa agaagcacag cagctttttg cagcgataga gcgtttgaaa gcgagcggaa aaggcattgt ctatgtctcc catcgcctgt cggaaatttt tcagattgcc gatagctata ccgtgtttcg cgacggcagt tatatcgata gcggcgatct cggtggcatc acccgtgcgg aactgatcca tatgatcgtc gggcgggaac tgggtgagga atatatcaag acgaatgcgc ctacggaaat accgggcgtc gaggtttccg gcctcagcgc gcccggcaag ataaccgaca tttcctttac cgcgcataag ggcgagattt ttggcattta cgggttgatg gggtcaggcc gctcggaaat attcaactgt cttttcggat tggagaaagc ctcccaagga acaattcggc ttgaaggcga acccgtcacc gtccggcgcc ccgcggacgc catggctctg gggctggctt ttgtgacgga agatcgcaag ctgaccggcc tcaatctcgg cgacagcgtt cgcaacaata tatgccttgc cagccttccc gagatgagtc cgcatttttc aatgaatcgg cgtgcggaat cggccgccag cagggagatg atcgagcgtt tcaggatcaa ggttgcgcac gacacgatgg ccgtttcggg cctttccggc ggcaaccagc agaaggtcgt gctcggaaaa tggttcctgc gaaagccacg cgttctgctg ctcgatgaac ccactcgcgg tgtcgatgtg ggtgcaaaac aggaaattta tcgcatcatt tgcgaatttg ccgcccaagg cgggaccgtc gtcatggttt cttccgagat tgatgaagtc ctcggcatgt ctgaccgcat catggtcatg cgtggcggac aatcggtcgg catctaccgc agggcggaaa cggacgcgca gtcattggtg catctttcga catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat