SSGCID: Target Page

BrmeA.10014.c

Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1)

CENTER ID:	BrmeA.10014.c
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.10014.c.B1.GE36688	Full length( BrmeA.10014.c )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_0285
BV-BRC:	fig\|224914.11.peg.285
RefSeq:	NP_539179.1
UniProt:	Q8YJ25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMQAETMQKK QPLLSVEKVE TYYGNICALK GIDMTVDEGE IVALIGANGA GKSTLMMTIF GSPRARTGRI LFNGKDITSM PPHEIAKLRI AQSPEGRRIF PRMTVLENLQ MGASLDNQQY FDEDVKLMFD LFPRLKERIN QRGGTLSGGE QQMLAIARAL MVRPKLLLLD EPSLGLAPLI VKQIFEAIKE LNRTQGLTVF LVEQNAFGAL KLADRGYVMV NGSITMSGSG RELLSDPEVR AAYLEGGRH

NT Sequence

ATGATGCAGG CTGAAACCAT GCAGAAAAAG CAACCGCTCC TATCCGTCGA GAAGGTCGAG ACCTATTACG GCAATATCTG TGCGCTCAAG GGCATCGATA TGACCGTGGA TGAGGGTGAG ATCGTGGCGC TGATCGGCGC CAACGGTGCG GGCAAATCCA CATTGATGAT GACGATCTTC GGCTCGCCGC GCGCGCGCAC CGGTCGCATT CTCTTCAATG GCAAGGACAT TACCTCCATG CCGCCGCACG AGATTGCGAA GCTACGTATT GCGCAGTCGC CGGAAGGCCG TCGCATCTTC CCGCGCATGA CGGTTCTGGA AAACCTCCAG ATGGGCGCGA GCCTCGATAA TCAGCAATAT TTCGATGAAG ACGTAAAGCT GATGTTCGAT CTGTTCCCGC GTCTGAAGGA GCGTATCAAC CAGCGCGGCG GCACGCTTTC GGGCGGCGAA CAGCAGATGC TGGCCATTGC GCGCGCGCTG ATGGTGCGTC CGAAGCTCCT GCTTCTCGAT GAGCCGTCAC TGGGTCTTGC GCCGCTGATC GTGAAGCAGA TCTTCGAGGC CATCAAGGAA TTGAACCGCA CACAGGGTTT GACGGTATTC CTCGTCGAAC AGAACGCATT CGGTGCGCTG AAGCTTGCCG ACCGCGGCTA TGTGATGGTC AACGGATCAA TCACGATGAG TGGTTCGGGA CGTGAACTTT TGTCCGACCC GGAAGTGCGT GCGGCTTATC TCGAAGGCGG AAGGCAT

Details for BrmeA.10014.c.B1.GE36688

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	30kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMM QAETMQKKQP LLSVEKVETY YGNICALKGI DMTVDEGEIV ALIGANGAGK STLMMTIFGS PRARTGRILF NGKDITSMPP HEIAKLRIAQ SPEGRRIFPR MTVLENLQMG ASLDNQQYFD EDVKLMFDLF PRLKERINQR GGTLSGGEQQ MLAIARALMV RPKLLLLDEP SLGLAPLIVK QIFEAIKELN RTQGLTVFLV EQNAFGALKL ADRGYVMVNG SITMSGSGRE LLSDPEVRAA YLEGGRH

Validated NT Sequence

cagccggntc ctatcttact cacttaatgc cttccgcctt cgagataagc cgcacgcact tccgggtcgg acaaaagttc acgtcccgaa ccactcatcg tgattgatcc gttgaccatc acatagccgc ggtcggcaag cttcagcgca ccgaatgcgt tctgttcgac gaggaatacc gtcaaaccct gtgtgcggtt caattccttg atggcctcga agatctgctt cacgatcagc ggcgcaagac ccagtgacgg ctcatcgaga agcaggagct tcggacgcac catcagcgcg cgcgcaatgg ccagcatctg ctgttcgccg cccgaaagcg tgccgccgcg ctggttgata cgctccttca gacgcgggaa cagatcgaac atcagcttta cgtcttcatc gaaatattgc tgattatcga ggctcgcgcc catctggagg ttttccagaa ccgtcatgcg cgggaagatg cgacggcctt ccggcgactg cgcaatacgt agcttcgcaa tctcgtgcgg cggcatggag gtaatgtcct tgccattgaa gagaatgcga ccggtgcgcg cgcgcggcga gccgaagatc gtcatcatca atgtggattt gcccgcaccg ttggcgccga tcagcgccac gatctcaccc tcatccacgg tcatatcgat gcccttgagc gcacagatat tgccgtaata ggtctcgacc ttctcgacgg ataggagcgg ttgctttttc tgcatggttt cagcctgcat catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgc aggctgaaac catgcagaaa aagcaaccgc tcctatccgt cgagaaggtc gagacctatt acggcaatat ctgtgcgctc aagggcatcg atatgaccgt ggatgagggt gagatcgtgg cgctgatcgg cgccaacggt gcgggcaaat ccacattgat gatgacgatc ttcggctcgc cgcgcgcgcg caccggtcgc attctcttca atggcaagga cattacctcc atgccgccgc acgagattgc gaagctacgt attgcgcagt cgccggaagg ccgtcgcatc ttcccgcgca tgacggttct ggaaaacctc cagatgggcg cgagcctcga taatcagcaa tatttcgatg aagacgtaaa gctgatgttc gatctgttcc cgcgtctgaa ggagcgtatc aaccagcgcg gcggcacgct ttcgggcggc gaacagcaga tgctggccat tgcgcgcgcg ctgatggtgc gtccgaagct cctgcttctc gatgagccgt cactgggtct tgcgccgctg atcgtgaagc agatcttcga ggccatcaag gaattgaacc gcacacaggg tttgacggta ttcctcgtcg aacagaacgc attcggtgcg ctgaagcttg ccgaccgcgg ctatgtgatg gtcaacggat caatcacgat gagtggttcg ggacgtgaac ttttgtccga cccggaagtg cgtgcggctt atctcgaagg cggaaggcat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat