BrmeA.01370.a
Phosphomannomutase (EC 5.4.2.8)
| CENTER ID: | BrmeA.01370.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01370.a.A1.GE33715 | full length | 1 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01370.a.A1.PS01305 | full length | 1 | 477 |
Structures
4HJH
DEPOSITED: 10/12/2012
DETERMINATION: XRay
CLONE: BrmeA.01370.a.A1.GE33715
PROTEIN: BrmeA.01370.a.A1.PS01305
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_163262 |
| PATRIC ID: | fig|224914.11.peg.3243 |
| RefSeq: | NP_541877.1 |
| UniProt: | Q8YBK1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSNSLKFGT SGLRGLAVEL NGLPAYAYTM AFVQMLAAKG QLQKGDKVFV GRDLRPSSPD IAALAMGAIE DAGFTPVNCG VLPTPALSYY AMGAKAPSIM VTGSHIPDDR NGLKFYRRDG EIDKDDEAAI SAAYRKLPAI LAARKHVGST ETDAALQAYA DRYAGFLGKG SLNGLRVGVY QHSSVARDLL MYLLTTLGVE PVALGRSDIF VPVDTEALRP EDIALLAQWG KSDRLDAIVS TDGDADRPLI ADEHGQFVRG DLAGAITATW VGADTLVTPV TSNTALESRF PKVLRTRVGS PYVIASMAQV STGNSGPVIG FEANGGVLLG STVERNGRSL TALPTRDALL PILACLATVH EKKTPLSTIA RSYGFRVALS DRLQNIPQEA STAFLALLED ADKRASLFPA GDAIVRVETI DGVKLFFQSG NAVHYRASGN APELRCYVES SDDTQAAKLQ ALGLEIARKA LKDATRP
NT Sequence
atgagcagca attccctcaa atttggcacg agcggccttc gcgggctggc ggtagaactg aacggcctgc ccgcctatgc ctatacgatg gcctttgtgc agatgcttgc tgcaaaaggg cagttgcaga agggcgacaa ggtgtttgtc ggcagggatt tgcgcccctc cagccctgat attgcagccc ttgccatggg tgccatcgaa gatgccggct tcacaccggt caattgcggc gtcctgccca cgcctgcgct gagctattat gcgatgggcg cgaaagcacc cagcatcatg gtcacgggaa gccatattcc agatgatcgc aacgggctga aattctatcg ccgcgacggt gaaatcgaca aggatgatga ggcggcaatc agtgcagcct atcgcaagct gcctgccatt ctcgctgccc gcaaacatgt cggctccacc gaaaccgatg cggccttgca ggcttatgcc gatcgctatg caggttttct tgggaaaggg agcctgaatg gcctgcgggt cggcgtttat caacattctt ccgtggcgcg cgatcttctg atgtacctgc tcacgacact cggcgtggaa cccgtggcgc tcggacgatc cgatatattc gtgccggtcg ataccgaggc attgcgcccc gaagacattg cgctgcttgc ccaatggggc aaaagcgaca ggcttgatgc catcgtctcc accgacggag acgcggatcg cccgctgatt gccgatgagc atggacaatt cgttcgcggc gatcttgctg gcgccatcac cgccacatgg gtgggggcgg atacgctcgt cacgccagtc acctccaaca ccgcattgga aagccgcttt cccaaggttt tgagaacgcg cgtcggttcg ccttatgtca tcgcaagcat ggcacaggta tccacgggca attccggccc ggtcatcggg tttgaggcca atggcggcgt tctgcttggc agcacggtcg agaggaatgg acgaagcctg acggccctgc cgacgcgcga cgccttgttg cccattctgg cttgccttgc cacggttcac gaaaagaaaa cgccgctttc aacaatcgcc cggtcctatg gcttccgcgt cgcgcttagc gaccggctgc aaaacattcc gcaggaggcg agcaccgcct tcctcgcgct cttggaggat gcggataaac gcgcctcgct ctttcctgct ggcgacgcaa tcgtgcgggt ggaaaccatc gacggcgtga agcttttctt tcaatcaggc aatgcggttc attatcgggc atcgggcaat gcgccggaac tgcgctgcta tgtggaatct tcggatgaca cacaagccgc caagcttcag gcgcttggct tggaaatcgc acgcaaagca ctaaaggatg cgacgaggcc atga
Details for BrmeA.01370.a.A1.GE33715
| HARVESTED ON: | 9/28/2011 |
| SEQUENCED ON: | 7/26/2016 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MGTLEAQTQG PGSMSSNSLK FGTSGLRGLA VELNGLPAYA YTMAFVQMLA AKGQLQKGDK VFVGRDLRPS SPDIAALAMG AIEDAGFTPV NCGVLPTPAL SYYAMGAKAP SIMVTGSHIP DDRNGLKFYR RDGEIDKDDE AAISAAYRKL PAILAARKHV GSTETDAALQ AYADRYAGFL GKGSLNGLRV GVYQHSSVAR DLLMYLLTTL GVEPVALGRS DIFVPVDTEA LRPEDIALLA QWGKSDRLDA IVXXDXXADR PLIADXXGQF VRGDLAGAIX ATWVGADTLX TPVTXNTAXE SRFPKVLRTR VGSPYVIASM AQVSTGNSGP VIGFEANGGV LLGSTVERNG RSLTALPTRD ALLPILACLA TVHEKKTPLS TIARSYGFRV ALSDRLQNIP QEASTAFLAL LEDADKRASL FPAGDAIVRV ETIDGVKLFF QSGNAVHYRA SGNAPELRCY VESSDDTQAA KLQALGLEIA RKALKDATRP
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagcagc aattccctca aatttggcac gagcggcctt cgcgggctgg cggtagaact gaacggcctg cccgcctatg cctatacgat ggcctttgtg cagatgcttg ctgcaaaagg gcagttgcag aagggcgaca aggtgtttgt cggcagggat ttgcgcccct ccagccctga tattgcagcc cttgccatgg gtgccatcga agatgccggc ttcacaccgg tcaattgcgg cgtcctgccc acgcctgcgc tgagctatta tgcgatgggc gcgaaagcac ccagcatcat ggtcacggga agccatattc cagatgatcg caacgggctg aaattctatc gccgcgacgg tgaaatcgac aaggatgatg aggcggcaat cagtgcagcc tatcgcaagc tgcctgccat tctcgctgcc cgcaaacatg tcggctccac cgaaaccgat gcggccttgc aggcttatgc cgatcgctat gcaggttttc ttgggaaagg gagcctgaat ggcctgcggg tcggcgttta tcaacattct tccgtggcgc gcgatcttct gatgtacctg ctcacgacac tcggcgtgga acccgtggcg ctcggacgat ccgatatatt cgtgccggtc gataccgagg cattgcgccc cgaagacatt gcgctgcttg cccaatgggg caaaagcgac aggcttgatg ccatcgtctn nnncgacnnn nncgcggatc gcccgctgat tgccgatgnn natggacaat tcgttcgcgg cgatcttgct ggcgccatca ncgccacatg ggtgggggcg gatacgctcg ncacgccagt cacntncaac accgcatngg aaagccgctt tcccaaggtt ttgagaacgc gcgtcggttc gccttatgtc atcgcaagca tggcacaggt atccacgggc aattccggcc cggtcatcgg gtttgaggcc aatggcggcg ttctgcttgg cagcacggtc gagaggaatg gacgaagcct gacggccctg ccgacgcgcg acgccttgtt gcccattctg gcttgccttg ccacggttca cgaaaagaaa acgccgcttt caacaatcgc ccggtcctat ggcttccgcg tcgcgcttag cgaccggctg caaaacattc cgcaggaggc gagcaccgcc ttcctcgcgc tcttggagga tgcggataaa cgcgcctcgc tctttcctgc tggcgacgca atcgtgcggg tggaaaccat cgacggcgtg aagcttttct ttcaatcagg caatgcggtt cattatcggg catcgggcaa tgcgccggaa ctgcgctgct atgtggaatc ttcggatgac acacaagccg ccaagcttca ggcgcttggc ttggaaatcg cacgcaaagc actaaaggat gcgacgaggc cataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSNSLKFG TSGLRGLAVE LNGLPAYAYT MAFVQMLAAK GQLQKGDKVF VGRDLRPSSP DIAALAMGAI EDAGFTPVNC GVLPTPALSY YAMGAKAPSI MVTGSHIPDD RNGLKFYRRD GEIDKDDEAA ISAAYRKLPA ILAARKHVGS TETDAALQAY ADRYAGFLGK GSLNGLRVGV YQHSSVARDL LMYLLTTLGV EPVALGRSDI FVPVDTEALR PEDIALLAQW GKSDRLDAIV STDGDADRPL IADEHGQFVR GDLAGAITAT WVGADTLVTP VTSNTALESR FPKVLRTRVG SPYVIASMAQ VSTGNSGPVI GFEANGGVLL GSTVERNGRS LTALPTRDAL LPILACLATV HEKKTPLSTI ARSYGFRVAL SDRLQNIPQE ASTAFLALLE DADKRASLFP AGDAIVRVET IDGVKLFFQS GNAVHYRASG NAPELRCYVE SSDDTQAAKL QALGLEIARK ALKDATRP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcagcaat tccctcaaat ttggcacgag cggccttcgc gggctggcgg tagaactgaa cggcctgccc gcctatgcct atacgatggc ctttgtgcag atgcttgctg caaaagggca gttgcagaag ggcgacaagg tgtttgtcgg cagggatttg cgcccctcca gccctgatat tgcagccctt gccatgggtg ccatcgaaga tgccggcttc acaccggtca attgcggcgt cctgcccacg cctgcgctga gctattatgc gatgggcgcg aaagcaccca gcatcatggt cacgggaagc catattccag atgatcgcaa cgggctgaaa ttctatcgcc gcgacggtga aatcgacaag gatgatgagg cggcaatcag tgcagcctat cgcaagctgc ctgccattct cgctgcccgc aaacatgtcg gctccaccga aaccgatgcg gccttgcagg cttatgccga tcgctatgca ggttttcttg ggaaagggag cctgaatggc ctgcgggtcg gcgtttatca acattcttcc gtggcgcgcg atcttctgat gtacctgctc acgacactcg gcgtggaacc cgtggcgctc ggacgatccg atatattcgt gccggtcgat accgaggcat tgcgccccga agacattgcg ctgcttgccc aatggggcaa aagcgacagg cttgatgcca tcgtctccac cgacggagac gcggatcgcc cgctgattgc cgatgagcat ggacaattcg ttcgcggcga tcttgctggc gccatcaccg ccacatgggt gggggcggat acgctcgtca cgccagtcac ctccaacacc gcattggaaa gccgctttcc caaggttttg agaacgcgcg tcggttcgcc ttatgtcatc gcaagcatgg cacaggtatc cacgggcaat tccggcccgg tcatcgggtt tgaggccaat ggcggcgttc tgcttggcag cacggtcgag aggaatggac gaagcctgac ggccctgccg acgcgcgacg ccttgttgcc cattctggct tgccttgcca cggttcacga aaagaaaacg ccgctttcaa caatcgcccg gtcctatggc ttccgcgtcg cgcttagcga ccggctgcaa aacattccgc aggaggcgag caccgccttc ctcgcgctct tggaggatgc ggataaacgc gcctcgctct ttcctgctgg cgacgcaatc gtgcgggtgg aaaccatcga cggcgtgaag cttttctttc aatcaggcaa tgcggttcat tatcgggcat cgggcaatgc gccggaactg cgctgctatg tggaatcttc ggatgacaca caagccgcca agcttcaggc gcttggcttg gaaatcgcac gcaaagcact aaaggatgcg acgaggccat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrmeA.01370.a.A1.PS01305
| PURIFICATION DATe: | 12/14/2011 |
| CONCENTRATION: | 44.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSSNSLK FGTSGLRGLA VELNGLPAYA YTMAFVQMLA AKGQLQKGDK VFVGRDLRPS SPDIAALAMG AIEDAGFTPV NCGVLPTPAL SYYAMGAKAP SIMVTGSHIP DDRNGLKFYR RDGEIDKDDE AAISAAYRKL PAILAARKHV GSTETDAALQ AYADRYAGFL GKGSLNGLRV GVYQHSSVAR DLLMYLLTTL GVEPVALGRS DIFVPVDTEA LRPEDIALLA QWGKSDRLDA IVXXDXXADR PLIADXXGQF VRGDLAGAIX ATWVGADTLX TPVTXNTAXE SRFPKVLRTR VGSPYVIASM AQVSTGNSGP VIGFEANGGV LLGSTVERNG RSLTALPTRD ALLPILACLA TVHEKKTPLS TIARSYGFRV ALSDRLQNIP QEASTAFLAL LEDADKRASL FPAGDAIVRV ETIDGVKLFF QSGNAVHYRA SGNAPELRCY VESSDDTQAA KLQALGLEIA RKALKDATRP
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagcagc aattccctca aatttggcac gagcggcctt cgcgggctgg cggtagaact gaacggcctg cccgcctatg cctatacgat ggcctttgtg cagatgcttg ctgcaaaagg gcagttgcag aagggcgaca aggtgtttgt cggcagggat ttgcgcccct ccagccctga tattgcagcc cttgccatgg gtgccatcga agatgccggc ttcacaccgg tcaattgcgg cgtcctgccc acgcctgcgc tgagctatta tgcgatgggc gcgaaagcac ccagcatcat ggtcacggga agccatattc cagatgatcg caacgggctg aaattctatc gccgcgacgg tgaaatcgac aaggatgatg aggcggcaat cagtgcagcc tatcgcaagc tgcctgccat tctcgctgcc cgcaaacatg tcggctccac cgaaaccgat gcggccttgc aggcttatgc cgatcgctat gcaggttttc ttgggaaagg gagcctgaat ggcctgcggg tcggcgttta tcaacattct tccgtggcgc gcgatcttct gatgtacctg ctcacgacac tcggcgtgga acccgtggcg ctcggacgat ccgatatatt cgtgccggtc gataccgagg cattgcgccc cgaagacatt gcgctgcttg cccaatgggg caaaagcgac aggcttgatg ccatcgtctn nnncgacnnn nncgcggatc gcccgctgat tgccgatgnn natggacaat tcgttcgcgg cgatcttgct ggcgccatca ncgccacatg ggtgggggcg gatacgctcg ncacgccagt cacntncaac accgcatngg aaagccgctt tcccaaggtt ttgagaacgc gcgtcggttc gccttatgtc atcgcaagca tggcacaggt atccacgggc aattccggcc cggtcatcgg gtttgaggcc aatggcggcg ttctgcttgg cagcacggtc gagaggaatg gacgaagcct gacggccctg ccgacgcgcg acgccttgtt gcccattctg gcttgccttg ccacggttca cgaaaagaaa acgccgcttt caacaatcgc ccggtcctat ggcttccgcg tcgcgcttag cgaccggctg caaaacattc cgcaggaggc gagcaccgcc ttcctcgcgc tcttggagga tgcggataaa cgcgcctcgc tctttcctgc tggcgacgca atcgtgcggg tggaaaccat cgacggcgtg aagcttttct ttcaatcagg caatgcggtt cattatcggg catcgggcaa tgcgccggaa ctgcgctgct atgtggaatc ttcggatgac acacaagccg ccaagcttca ggcgcttggc ttggaaatcg cacgcaaagc actaaaggat gcgacgaggc cataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSNSLKFG TSGLRGLAVE LNGLPAYAYT MAFVQMLAAK GQLQKGDKVF VGRDLRPSSP DIAALAMGAI EDAGFTPVNC GVLPTPALSY YAMGAKAPSI MVTGSHIPDD RNGLKFYRRD GEIDKDDEAA ISAAYRKLPA ILAARKHVGS TETDAALQAY ADRYAGFLGK GSLNGLRVGV YQHSSVARDL LMYLLTTLGV EPVALGRSDI FVPVDTEALR PEDIALLAQW GKSDRLDAIV STDGDADRPL IADEHGQFVR GDLAGAITAT WVGADTLVTP VTSNTALESR FPKVLRTRVG SPYVIASMAQ VSTGNSGPVI GFEANGGVLL GSTVERNGRS LTALPTRDAL LPILACLATV HEKKTPLSTI ARSYGFRVAL SDRLQNIPQE ASTAFLALLE DADKRASLFP AGDAIVRVET IDGVKLFFQS GNAVHYRASG NAPELRCYVE SSDDTQAAKL QALGLEIARK ALKDATRP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAGCAAT TCCCTCAAAT TTGGCACGAG CGGCCTTCGC GGGCTGGCGG TAGAACTGAA CGGCCTGCCC GCCTATGCCT ATACGATGGC CTTTGTGCAG ATGCTTGCTG CAAAAGGGCA GTTGCAGAAG GGCGACAAGG TGTTTGTCGG CAGGGATTTG CGCCCCTCCA GCCCTGATAT TGCAGCCCTT GCCATGGGTG CCATCGAAGA TGCCGGCTTC ACACCGGTCA ATTGCGGCGT CCTGCCCACG CCTGCGCTGA GCTATTATGC GATGGGCGCG AAAGCACCCA GCATCATGGT CACGGGAAGC CATATTCCAG ATGATCGCAA CGGGCTGAAA TTCTATCGCC GCGACGGTGA AATCGACAAG GATGATGAGG CGGCAATCAG TGCAGCCTAT CGCAAGCTGC CTGCCATTCT CGCTGCCCGC AAACATGTCG GCTCCACCGA AACCGATGCG GCCTTGCAGG CTTATGCCGA TCGCTATGCA GGTTTTCTTG GGAAAGGGAG CCTGAATGGC CTGCGGGTCG GCGTTTATCA ACATTCTTCC GTGGCGCGCG ATCTTCTGAT GTACCTGCTC ACGACACTCG GCGTGGAACC CGTGGCGCTC GGACGATCCG ATATATTCGT GCCGGTCGAT ACCGAGGCAT TGCGCCCCGA AGACATTGCG CTGCTTGCCC AATGGGGCAA AAGCGACAGG CTTGATGCCA TCGTCTCCAC CGACGGAGAC GCGGATCGCC CGCTGATTGC CGATGAGCAT GGACAATTCG TTCGCGGCGA TCTTGCTGGC GCCATCACCG CCACATGGGT GGGGGCGGAT ACGCTCGTCA CGCCAGTCAC CTCCAACACC GCATTGGAAA GCCGCTTTCC CAAGGTTTTG AGAACGCGCG TCGGTTCGCC TTATGTCATC GCAAGCATGG CACAGGTATC CACGGGCAAT TCCGGCCCGG TCATCGGGTT TGAGGCCAAT GGCGGCGTTC TGCTTGGCAG CACGGTCGAG AGGAATGGAC GAAGCCTGAC GGCCCTGCCG ACGCGCGACG CCTTGTTGCC CATTCTGGCT TGCCTTGCCA CGGTTCACGA AAAGAAAACG CCGCTTTCAA CAATCGCCCG GTCCTATGGC TTCCGCGTCG CGCTTAGCGA CCGGCTGCAA AACATTCCGC AGGAGGCGAG CACCGCCTTC CTCGCGCTCT TGGAGGATGC GGATAAACGC GCCTCGCTCT TTCCTGCTGG CGACGCAATC GTGCGGGTGG AAACCATCGA CGGCGTGAAG CTTTTCTTTC AATCAGGCAA TGCGGTTCAT TATCGGGCAT CGGGCAATGC GCCGGAACTG CGCTGCTATG TGGAATCTTC GGATGACACA CAAGCCGCCA AGCTTCAGGC GCTTGGCTTG GAAATCGCAC GCAAAGCACT AAAGGATGCG ACGAGGCCAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA