SSGCID: Target Page

BrmeA.01147.a

Cysteine synthase (EC 2.5.1.47)

CENTER ID:	BrmeA.01147.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.01147.a.A1.GE33708	full length		1	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.1045
RefSeq:	NP_539850.1
UniProt:	Q8YH71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFNSVLDTIG NTPLIRLSKA SELTGCDIYG KAEFLNPGQS VKDRAALYII RDAEKRGLLR PGGVIVEGTA GNTGIGLTMV AKALGYRTAI VIPETQSQEK KEVLRLLGAE LIEVPAAPYR NPNNYVRLSG RLAEQLAKTE PNGAIWANQF DNTVNRQAHI ETTAQEIWRD TSDQIDGFVA AVGSGGTLAG TAIGLKERNH NIKIALADPH GAALHAFYTT GELKAEGDSI TEGIGQGRIT ANLEGFTPDF SYQIPDAEAL DILFALVEEE GLCLGGSSGI NIAGAIRLAK DLGPGHTIVT VLCDYGNRYQ SKLFNPAFLR GKSLPVPRWL EKKTEIDIPF EG

NT Sequence

atgttcaatt cggtactcga cacaatcggc aacacgccgt tgatccgcct cagcaaggct tccgagctca caggatgcga tatttacggg aaagcagagt ttctcaatcc gggccagtcg gtcaaggacc gtgcggcgct ctacattatc cgcgatgcgg aaaaacgcgg gcttttgcgg cccggaggcg tgattgtgga aggaaccgcg ggcaataccg gcattggcct cacgatggtc gccaaggcat tgggctaccg cacggcgatc gtgattcctg aaacgcagag ccaggaaaaa aaggaagtct tgcgcctgct cggtgcagag ctgatcgagg ttcccgccgc cccctatcgc aacccgaaca attatgtgcg cctttcaggg cgtcttgccg agcaactggc caaaaccgag ccgaatggcg caatatgggc caatcagttc gacaatacgg tcaaccggca agcccatatc gagaccacgg cgcaggaaat ctggcgcgat acaagcgacc agatcgatgg ttttgtggcc gctgtgggat ctggcggtac gcttgccgga acggcgattg gcctgaagga acggaaccac aatatcaaaa ttgctctggc ggacccgcat ggcgcggcgc ttcacgcctt ctatacgacc ggcgaattga aggcagaagg cgattcgatc accgaaggca tcgggcaggg gcgcatcacc gccaatcttg aaggcttcac ccctgatttt tcctaccaga ttcccgatgc ggaggcgctc gatatcctgt ttgccctcgt ggaagaggag gggctttgcc tcggcggctc gtccggcatc aacattgccg gtgcaatccg gcttgcaaaa gatctcgggc cgggccacac aattgtgacc gtgctttgtg actacggtaa tcgttaccag tcaaagctgt tcaatcccgc cttcctccgc ggcaagagcc tgcccgtgcc gcgctggctg gagaagaaaa ccgaaataga cataccgttc gaggggtaa

Details for BrmeA.01147.a.A1.GE33708

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	108

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFNSVLDTI GNTPLIRLSK ASELTGCDIY GKAEFLNPGQ SVKDRAALYI IRDAEKRGLL RPGGVIVEGT AGNTGIGLTM VAKALGYRTA IVIPETQSQE KKEVLRLLGA ELIEVPAAPY RNPNNYVRLS GRLAEQLAKT EPNGAIWANQ FDNTVNRQAH IETTAQEIWR DTSDQIDGFV AAVGSGGTLA GTAIGLKERN HNIKIALADP HGAALHAFYT TGELKAEGDS ITEGIGQGRI TANLEXFTPD FSXQIPDAXA LDILFALVEX EGLCLGGSSG INIXRCNPAC KRSRAGXTIV TXLXDYGXRY QSKLXXPXXX XXXXXXXXLV XXXXXXXXXX XGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX

Validated NT Sequence

ttnnttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttcaat tcggtactcg acacaatcgg caacacgccg ttgatccgcc tcagcaaggc ttccgagctc acaggatgcg atatttacgg gaaagcagag tttctcaatc cgggccagtc ggtcaaggac cgtgcggcgc tctacattat ccgcgatgcg gaaaaacgcg ggcttttgcg gcccggaggc gtgattgtgg aaggaaccgc gggcaatacc ggcattggcc tcacgatggt cgccaaggca ttgggctacc gcacggcgat cgtgattcct gaaacgcaga gccaggaaaa aaaggaagtc ttgcgcctgc tcggtgcaga gctgatcgag gttcccgccg ccccctatcg caacccgaac aattatgtgc gcctttcagg gcgtcttgcc gagcaactgg ccaaaaccga gccgaatggc gcaatatggg ccaatcagtt cgacaatacg gtcaaccggc aagcccatat cgagaccacg gcgcaggaaa tctggcgcga tacaagcgac cagatcgatg gttttgtggc cgctgtggga tctggcggta cgcttgccgg aacggcgatt ggcctgaagg aacggaacca caatatcaaa attgctctgg cggacccgca tggcgcggcg cttcacgcct tctatacgac cggcgaattg aaggcagaag gcgattcgat caccgaaggc atcgggcagg ggcgcatcac cgccaatctt gaangcttca cccctgattt ttcctancag attcccgatg cggangcgct cgatatcctg tttgccctcg tggaaganga ggggctttgc ctcggcggct cgtccggcat caacattgnc cggtgcaatc cggcttgcaa aagatctcgg gccgggcnca caattgtgac cgngctttnn gactacggta ntcgttacca gtcaaagctg ntcantcccn cntncnncnc ngnanannnt gncngnncnn nnctngtgnn nannaanncn aantanannn tancnnncnn nggnnnntna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctnnnn nannnnaann nntnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn annnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMFNSVLDTI GNTPLIRLSK ASELTGCDIY GKAEFLNPGQ SVKDRAALYI IRDAEKRGLL RPGGVIVEGT AGNTGIGLTM VAKALGYRTA IVIPETQSQE KKEVLRLLGA ELIEVPAAPY RNPNNYVRLS GRLAEQLAKT EPNGAIWANQ FDNTVNRQAH IETTAQEIWR DTSDQIDGFV AAVGSGGTLA GTAIGLKERN HNIKIALADP HGAALHAFYT TGELKAEGDS ITEGIGQGRI TANLEGFTPD FSYQIPDAEA LDILFALVEE EGLCLGGSSG INIAGAIRLA KDLGPGHTIV TVLCDYGNRY QSKLFNPAFL RGKSLPVPRW LEKKTEIDIP FEG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcaattcg gtactcgaca caatcggcaa cacgccgttg atccgcctca gcaaggcttc cgagctcaca ggatgcgata tttacgggaa agcagagttt ctcaatccgg gccagtcggt caaggaccgt gcggcgctct acattatccg cgatgcggaa aaacgcgggc ttttgcggcc cggaggcgtg attgtggaag gaaccgcggg caataccggc attggcctca cgatggtcgc caaggcattg ggctaccgca cggcgatcgt gattcctgaa acgcagagcc aggaaaaaaa ggaagtcttg cgcctgctcg gtgcagagct gatcgaggtt cccgccgccc cctatcgcaa cccgaacaat tatgtgcgcc tttcagggcg tcttgccgag caactggcca aaaccgagcc gaatggcgca atatgggcca atcagttcga caatacggtc aaccggcaag cccatatcga gaccacggcg caggaaatct ggcgcgatac aagcgaccag atcgatggtt ttgtggccgc tgtgggatct ggcggtacgc ttgccggaac ggcgattggc ctgaaggaac ggaaccacaa tatcaaaatt gctctggcgg acccgcatgg cgcggcgctt cacgccttct atacgaccgg cgaattgaag gcagaaggcg attcgatcac cgaaggcatc gggcaggggc gcatcaccgc caatcttgaa ggcttcaccc ctgatttttc ctaccagatt cccgatgcgg aggcgctcga tatcctgttt gccctcgtgg aagaggaggg gctttgcctc ggcggctcgt ccggcatcaa cattgccggt gcaatccggc ttgcaaaaga tctcgggccg ggccacacaa ttgtgaccgt gctttgtgac tacggtaatc gttaccagtc aaagctgttc aatcccgcct tcctccgcgg caagagcctg cccgtgccgc gctggctgga gaagaaaacc gaaatagaca taccgttcga ggggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa