BrmeA.01059.a
Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2)
| CENTER ID: | BrmeA.01059.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01059.a.A1.GE33722 | full length | 1 | 205 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01059.a.A1.PS01278 | full length | 1 | 205 |
Structures
4DS3
DEPOSITED: 2/17/2012
DETERMINATION: XRay
CLONE: BrmeA.01059.a.A1.GE33722
PROTEIN: BrmeA.01059.a.A1.PS01278
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129694 |
| PATRIC ID: | fig|224914.11.peg.1392 |
| RefSeq: | NP_540158.1 |
| UniProt: | Q8YGB8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRNRVVIFI SGGGSNMEAL IRAAQAPGFP AEIVAVFSDK AEAGGLAKAE AAGIATQVFK RKDFASKEAH EDAILAALDV LKPDIICLAG YMRLLSGRFI APYEGRILNI HPSLLPLFPG LHTHQRALDA GMKLAGCTVH LVTEGMDEGP ILAQAAVPVL DGDTAETLAA RVLKAEHRLY PLALQKFAAG EKASNQFSDG MVLSA
NT Sequence
atgaaacgca accgggtcgt catctttatt tcaggcggcg gctccaacat ggaagcgctg atccgcgcgg cacaagcgcc cggttttccg gcggaaatcg tagcggtctt ttccgacaag gcagaagcgg gcggccttgc caaggcagaa gcggcaggca ttgccacgca ggttttcaag cgcaaggact ttgcttccaa agaagcgcat gaggatgcga tccttgccgc tctcgacgtt ctcaagccgg atattatctg ccttgcaggc tatatgcgcc ttctgtcagg ccgctttatt gccccttacg aggggcgcat tcttaatatc cacccttccc tgctaccgct ttttccgggc ctgcacacgc accagcgcgc gctcgatgcg ggcatgaagc ttgcgggctg caccgtccat ctggtgacgg aaggcatgga cgaagggcca atcctcgcac aagccgcagt gccggtgctg gacggcgata cagccgaaac gctggccgcg cgcgtgctga aggccgaaca ccggctttat ccgcttgcgc ttcaaaagtt tgcggctgga gagaaggctt ccaaccagtt ttcggacggc atggtgctca gcgcctga
Details for BrmeA.01059.a.A1.GE33722
| HARVESTED ON: | 9/28/2011 |
| SEQUENCED ON: | 10/4/2011 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKRNRVVIF ISGGGSNMEA LIRAAQAPGF PAEIVAVFSD KAEAGGLAKA EAAGIATQVF KRKDFASKEA HEDAILAALD VLKPDIICLA GYMRLLSGRF IAPYEGRILN IHPSLLPLFP GLHTHQRALD AGMKLAGCTV HLVTEGMDEG PILAQAAVPV LDGDTAETLA ARVLKAEHRL YPLALQKFAA GEKASNQFSD GMVLSA
Validated NT Sequence
ttattaggcg ctgagcacca tgccgtccga aaactggttg gaagccttct ctccagccgc aaacttttga agcgcaagcg gataaagccg gtgttcggcc ttcagcacgc gcgcggccag cgtttcggct gtatcgccgt ccagcaccgg cactgcggct tgtgcgagga ttggcccttc gtccatgcct tccgtcacca gatggacggt gcagcccgca agcttcatgc ccgcatcgag cgcgcgctgg tgcgtgtgca ggcccggaaa aagcggtagc agggaagggt ggatattaag aatgcgcccc tcgtaagggg caataaagcg gcctgacaga aggcgcatat agcctgcaag gcagataata tccggcttga gaacgtcgag agcggcaagg atcgcatcct catgcgcttc tttggaagca aagtccttgc gcttgaaaac ctgcgtggca atgcctgccg cttctgcctt ggcaaggccg cccgcttctg ccttgtcgga aaagaccgct acgatttccg ccggaaaacc gggcgcttgt gccgcgcgga tcagcgcttc catgttggag ccgccgcctg aaataaagat gacgacccgg ttgcgtttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKRNRVVIF ISGGGSNMEA LIRAAQAPGF PAEIVAVFSD KAEAGGLAKA EAAGIATQVF KRKDFASKEA HEDAILAALD VLKPDIICLA GYMRLLSGRF IAPYEGRILN IHPSLLPLFP GLHTHQRALD AGMKLAGCTV HLVTEGMDEG PILAQAAVPV LDGDTAETLA ARVLKAEHRL YPLALQKFAA GEKASNQFSD GMVLSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacgcaac cgggtcgtca tctttatttc aggcggcggc tccaacatgg aagcgctgat ccgcgcggca caagcgcccg gttttccggc ggaaatcgta gcggtctttt ccgacaaggc agaagcgggc ggccttgcca aggcagaagc ggcaggcatt gccacgcagg ttttcaagcg caaggacttt gcttccaaag aagcgcatga ggatgcgatc cttgccgctc tcgacgttct caagccggat attatctgcc ttgcaggcta tatgcgcctt ctgtcaggcc gctttattgc cccttacgag gggcgcattc ttaatatcca cccttccctg ctaccgcttt ttccgggcct gcacacgcac cagcgcgcgc tcgatgcggg catgaagctt gcgggctgca ccgtccatct ggtgacggaa ggcatggacg aagggccaat cctcgcacaa gccgcagtgc cggtgctgga cggcgataca gccgaaacgc tggccgcgcg cgtgctgaag gccgaacacc ggctttatcc gcttgcgctt caaaagtttg cggctggaga gaaggcttcc aaccagtttt cggacggcat ggtgctcagc gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrmeA.01059.a.A1.PS01278
| PURIFICATION DATe: | 11/10/2011 |
| CONCENTRATION: | 81.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKRNRVVIF ISGGGSNMEA LIRAAQAPGF PAEIVAVFSD KAEAGGLAKA EAAGIATQVF KRKDFASKEA HEDAILAALD VLKPDIICLA GYMRLLSGRF IAPYEGRILN IHPSLLPLFP GLHTHQRALD AGMKLAGCTV HLVTEGMDEG PILAQAAVPV LDGDTAETLA ARVLKAEHRL YPLALQKFAA GEKASNQFSD GMVLSA
Validated NT Sequence
ttattaggcg ctgagcacca tgccgtccga aaactggttg gaagccttct ctccagccgc aaacttttga agcgcaagcg gataaagccg gtgttcggcc ttcagcacgc gcgcggccag cgtttcggct gtatcgccgt ccagcaccgg cactgcggct tgtgcgagga ttggcccttc gtccatgcct tccgtcacca gatggacggt gcagcccgca agcttcatgc ccgcatcgag cgcgcgctgg tgcgtgtgca ggcccggaaa aagcggtagc agggaagggt ggatattaag aatgcgcccc tcgtaagggg caataaagcg gcctgacaga aggcgcatat agcctgcaag gcagataata tccggcttga gaacgtcgag agcggcaagg atcgcatcct catgcgcttc tttggaagca aagtccttgc gcttgaaaac ctgcgtggca atgcctgccg cttctgcctt ggcaaggccg cccgcttctg ccttgtcgga aaagaccgct acgatttccg ccggaaaacc gggcgcttgt gccgcgcgga tcagcgcttc catgttggag ccgccgcctg aaataaagat gacgacccgg ttgcgtttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKRNRVVIF ISGGGSNMEA LIRAAQAPGF PAEIVAVFSD KAEAGGLAKA EAAGIATQVF KRKDFASKEA HEDAILAALD VLKPDIICLA GYMRLLSGRF IAPYEGRILN IHPSLLPLFP GLHTHQRALD AGMKLAGCTV HLVTEGMDEG PILAQAAVPV LDGDTAETLA ARVLKAEHRL YPLALQKFAA GEKASNQFSD GMVLSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACGCAAC CGGGTCGTCA TCTTTATTTC AGGCGGCGGC TCCAACATGG AAGCGCTGAT CCGCGCGGCA CAAGCGCCCG GTTTTCCGGC GGAAATCGTA GCGGTCTTTT CCGACAAGGC AGAAGCGGGC GGCCTTGCCA AGGCAGAAGC GGCAGGCATT GCCACGCAGG TTTTCAAGCG CAAGGACTTT GCTTCCAAAG AAGCGCATGA GGATGCGATC CTTGCCGCTC TCGACGTTCT CAAGCCGGAT ATTATCTGCC TTGCAGGCTA TATGCGCCTT CTGTCAGGCC GCTTTATTGC CCCTTACGAG GGGCGCATTC TTAATATCCA CCCTTCCCTG CTACCGCTTT TTCCGGGCCT GCACACGCAC CAGCGCGCGC TCGATGCGGG CATGAAGCTT GCGGGCTGCA CCGTCCATCT GGTGACGGAA GGCATGGACG AAGGGCCAAT CCTCGCACAA GCCGCAGTGC CGGTGCTGGA CGGCGATACA GCCGAAACGC TGGCCGCGCG CGTGCTGAAG GCCGAACACC GGCTTTATCC GCTTGCGCTT CAAAAGTTTG CGGCTGGAGA GAAGGCTTCC AACCAGTTTT CGGACGGCAT GGTGCTCAGC GCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA