BrmeA.01048.a
Acyl-CoA dehydrogenase (EC 1.3.99.3)
| CENTER ID: | BrmeA.01048.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01048.a.A1.GE33714 | full length | 1 | 551 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.01048.a.A1.PS01389 | full length | 1 | 551 |
Structures
5EZ3
DEPOSITED: 11/26/2015
DETERMINATION: XRay
CLONE: BrmeA.01048.a.A1.GE33714
PROTEIN: BrmeA.01048.a.A1.PS01389
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132028 |
| PATRIC ID: | fig|224914.11.peg.3000 |
| RefSeq: | NP_541649.1 |
| UniProt: | Q8YC61 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQAKRNEPL KTHEVTNQTP PITGTNAYLG DPLLMQIAAR FPKELHTELE QAGRFVLSAE AQDLARLANT ELPKLRTHDR QGRRIDLVEY HPAYHALMRR SVAQGLHSSI WEDNPLESGR RHQARAARFY LTAQLEAGHL CPLTMTSASL AALMASPEVY KQWSPAVLSR KYDFSQKPAF RKQGVTLGMG MTEKQGGTDV RANATRAEPA IGGAWRLTGH KWFMSAPMSD AFLTLAQTKE GLSCFLLPRL GEKGESNGFF FQRLKDKLGN RSNASSEVEF DGALGQMIGS PGEGVKTIMD MVTLTRLDCA VASAGLMRSG LAEAVHHSRH RHVFGKPLVE QPLMQRVLAD MALDVAGATA LSMRLARAFD MAASDRAEAA FARSMTPVVK YWVCKIAPAL LYEAMECLGG NGYIEDGNLA RAYREAPVNA IWEGSGNVMA LDVARVLSRA PALFDGVLDW ISGQLGPRGQ GTIDVLRAAL QLTETDQGVA RLLTEQLAFA AAAAELRQLG ADDIADAFIE TRLGGLWRTT YGMLDARHNA MRIIDQLYPA S
NT Sequence
ttgaatcaag ccaaacggaa cgaacccttg aaaacgcacg aagtcaccaa ccagacgccg ccgatcaccg gcacgaacgc ttatctgggc gacccgcttc tcatgcagat tgcggcgcgt tttccaaaag agcttcatac agaactggag caggcgggcc gtttcgtact ttcggccgag gcgcaggatt tggctcgtct tgccaacacc gaattgccga agctgcgtac gcatgaccgg cagggccgcc gcatcgacct tgtcgaatat cacccggcct atcacgcgct gatgcgccgc tccgtggcgc aggggctgca ttcgtccata tgggaagaca atccgctgga gagtggccgc cgtcatcagg cgcgtgccgc ccgtttttat ctcactgcgc agcttgaggc cgggcacctt tgcccgctca ccatgacgag cgcctctctg gccgcactca tggcctcgcc ggaggtttac aagcaatggt ctccggcagt cctgtcgcgc aaatatgatt tttcgcaaaa gcctgccttc cgcaaacagg gtgttacgct tggcatgggc atgacggaaa aacagggcgg caccgatgtt cgcgccaacg ccacccgtgc cgaacctgcc attggcgggg catggcggct tacgggccat aaatggttca tgtcggcgcc gatgtcagat gcttttctga cactggcgca aacgaaggag gggctttcct gtttccttct gccgcgactg ggcgaaaagg gcgagagcaa tggctttttc ttccagcgcc tgaaagataa gctcggcaac agatccaatg cttcctcgga agtggaattt gacggggctt tgggccagat gatcggcagt ccgggcgagg gcgtgaagac gatcatggat atggtgacgt tgacgcggct tgattgcgcg gtagcttctg ccggacttat gcgctcaggg ctggctgagg ccgttcacca cagccgccac cgccatgtgt tcggcaagcc tttggtcgag cagccgctga tgcagcgcgt tctggccgat atggcgctgg acgtggcggg cgccacggcg ctttccatgc gtcttgcccg cgccttcgac atggcggcaa gcgaccgggc ggaagccgct tttgcgcgct ccatgacgcc ggtggtgaaa tattgggtct gcaagattgc gcctgcgctc ctttatgaag cgatggagtg ccttggcggc aacggctata tcgaagatgg caatctggcg cgggcctatc gtgaagcgcc ggtcaatgcg atatgggaag gttccggcaa tgtgatggcg ctggacgtgg cacgggtgct ctcgcgtgcg ccggcacttt tcgatggcgt gctggactgg atcagcggac aattgggccc gcgcgggcag ggcacgatcg acgtgctgcg cgccgccctt caactgaccg agacggatca gggtgtggca cgtcttttga ccgagcagct tgccttcgca gccgcagccg ctgaactgcg ccagcttggc gcggacgata ttgctgatgc ctttatcgaa acccgcctcg gcggcctgtg gcgcaccacc tacgggatgc tggatgcgcg ccacaatgcg atgcgtatca tcgaccagct ctatccggcg tcgtaa
Details for BrmeA.01048.a.A1.GE33714
| HARVESTED ON: | 9/28/2011 |
| SEQUENCED ON: | 10/4/2011 |
| EXPECTED MW: | 62kDa |
| OBSERVED MW: | 62kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MLNQAKRNEP LKTHEVTNQT PPITGTNAYL GDPLLMQIAA RFPKELHTEL EQAGRFVLSA EAQDLARLAN TELPKLRTHD RQGRRIDLVE YHPAYHALMR RSVAQGLHSS IWEDNPLESG RRHQARAARF YLTAQLEAGH LCPLTMTSAS LAALMASPEV YKQWSPAVLS RKYDFSQKPA FRKQGVTLGM GMTEKQGGTD VRANATRAEP AIGGAWRLTG HKWFMSAPMS DAFLTLAQTK XGLSCFLLPR LGEKGESNGF FFQRLKDKLG NRSNASSEVE FDGALGQMIG SPGEGVKTXM DMVTXTRLDC AVASAGLMRS GLAEAVHHSR HRHVFGKPLV EQPLMQRVLA DMALDVAGAT ALSMRLARAF DMAASDRAEA AFARSMTPVV KYWVCKIAPA LLYEAMECLG GNGYIEDGNL ARAYREAPVN AIWEGSGNVM ALDVARVLSR APALFDGVLD WISGQLGPRG QGTIDVLRAA LQLTETDQGV ARLLTEQLAF AAAAAELRQL GADDIADAFI ETRLGGLWRT TYGMLDARHN AMRIIDQLYP AS
Validated NT Sequence
acgacgccgg atagagctgg tcgatgatac gcatcgcatt gtggcgcgca tccagcatcc cgtaggtggt gcgccacagg ccgccgaggc gggtttcgat aaaggcatca gcaatatcgt ccgcgccaag ctggcgcagt tcagcggctg cggctgcgaa ggcaagctgc tcggtcaaaa gacgtgccac accctgatcc gtctcggtca gttgaagggc ggcgcgcagc acgtcgatcg tgccctgccc gcgcgggccc aattgtccgc tgatccagtc cagcacgcca tcgaaaagtg ccggcgcacg cgagagcacc cgtgccacgt ccagcgccat cacattgccg gaaccttccc atatcgcatt gaccggcgct tcacgatagg cccgcgccag attgccatct tcgatatagc cgttgccgcc aaggcactcc atcgcttcat aaaggagcgc aggcgcaatc ttgcagaccc aatatttcac caccggcgtc atggagcgcg caaaagcggc ttccgcccgg tcgcttgccg ccatgtcgaa ngcgcgggca agacgcatgg aaagcgccgt ggcgcccgcc acgtccagcg ccatatcggc cagaacgcgc tgcatcagcg gctgctcgac caaaggcttg ccgaacacat ggcggtggcg gctgtggtga acggcctcag ccagccctga gcgcataagt ccggcagaag ctaccgcgca atcaagccgc gtcnacgtca ccatatccat gancgtcttc acgccctcgc ccggactgcc gatcatctgg cccaaagccc cgtcaaattc cacttccgag gaagcattgg atctgttgcc gagcttatct ttcaggcgct ggaagaaaaa gccattgctc tcgccctttt cgcccagtcg cggcagaagg aaacaggaaa gcccntcctt cgtttgcgcc agtgtcagaa aagcatctga catcggcgcc gacatgaacc atttatggcc cgtaagccgc catgccccgc caatggcagg ttcggcacgg gtggcgttgg cgcgaacatc ggtgccgccc tgtttttccg tcatgcccat gccaagcgta acaccctgtt tgcggaaggc aggcttttgc gaaaaatcat atttgcgcga caggactgcc ggagaccatt gcttgtaaac ctccggcgag gccatgagtg cggccagaga ggcgctcgtc atggtgagcg ggcaaaggtg cccggcctca agctgcgcag tgagataaaa acgggcggca cgcgcctgat gacggcggcc actctccagc ggattgtctt cccatatgga cgaatgcagc ccctgcgcca cggagcggcg catcagcgcg tgataggccg ggtgatattc gacaaggtcg atgcggcggc cctgccggtc atgcgtacgc agcttcggca attcggtgtt ggcaagacga gccaaatcct gcgcctcggc cgaaagtacg aaacggcccg cctgctccag ttctgtatga agctcttttg gaaaacgcgc cgcaatctgc atgagaagcg ggtcgcccag ataagcgttc gtgccggtga tcggcggcgt ctggttggtg acttcgtgcg ttttcaaggg ttcgttccgt ttggcttgat tcaacatcga accaggaccc tgggtctgag cttccagggt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQAKRNE PLKTHEVTNQ TPPITGTNAY LGDPLLMQIA ARFPKELHTE LEQAGRFVLS AEAQDLARLA NTELPKLRTH DRQGRRIDLV EYHPAYHALM RRSVAQGLHS SIWEDNPLES GRRHQARAAR FYLTAQLEAG HLCPLTMTSA SLAALMASPE VYKQWSPAVL SRKYDFSQKP AFRKQGVTLG MGMTEKQGGT DVRANATRAE PAIGGAWRLT GHKWFMSAPM SDAFLTLAQT KEGLSCFLLP RLGEKGESNG FFFQRLKDKL GNRSNASSEV EFDGALGQMI GSPGEGVKTI MDMVTLTRLD CAVASAGLMR SGLAEAVHHS RHRHVFGKPL VEQPLMQRVL ADMALDVAGA TALSMRLARA FDMAASDRAE AAFARSMTPV VKYWVCKIAP ALLYEAMECL GGNGYIEDGN LARAYREAPV NAIWEGSGNV MALDVARVLS RAPALFDGVL DWISGQLGPR GQGTIDVLRA ALQLTETDQG VARLLTEQLA FAAAAAELRQ LGADDIADAF IETRLGGLWR TTYGMLDARH NAMRIIDQLY PAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaatcaa gccaaacgga acgaaccctt gaaaacgcac gaagtcacca accagacgcc gccgatcacc ggcacgaacg cttatctggg cgacccgctt ctcatgcaga ttgcggcgcg ttttccaaaa gagcttcata cagaactgga gcaggcgggc cgtttcgtac tttcggccga ggcgcaggat ttggctcgtc ttgccaacac cgaattgccg aagctgcgta cgcatgaccg gcagggccgc cgcatcgacc ttgtcgaata tcacccggcc tatcacgcgc tgatgcgccg ctccgtggcg caggggctgc attcgtccat atgggaagac aatccgctgg agagtggccg ccgtcatcag gcgcgtgccg cccgttttta tctcactgcg cagcttgagg ccgggcacct ttgcccgctc accatgacga gcgcctctct ggccgcactc atggcctcgc cggaggttta caagcaatgg tctccggcag tcctgtcgcg caaatatgat ttttcgcaaa agcctgcctt ccgcaaacag ggtgttacgc ttggcatggg catgacggaa aaacagggcg gcaccgatgt tcgcgccaac gccacccgtg ccgaacctgc cattggcggg gcatggcggc ttacgggcca taaatggttc atgtcggcgc cgatgtcaga tgcttttctg acactggcgc aaacgaagga ggggctttcc tgtttccttc tgccgcgact gggcgaaaag ggcgagagca atggcttttt cttccagcgc ctgaaagata agctcggcaa cagatccaat gcttcctcgg aagtggaatt tgacggggct ttgggccaga tgatcggcag tccgggcgag ggcgtgaaga cgatcatgga tatggtgacg ttgacgcggc ttgattgcgc ggtagcttct gccggactta tgcgctcagg gctggctgag gccgttcacc acagccgcca ccgccatgtg ttcggcaagc ctttggtcga gcagccgctg atgcagcgcg ttctggccga tatggcgctg gacgtggcgg gcgccacggc gctttccatg cgtcttgccc gcgccttcga catggcggca agcgaccggg cggaagccgc ttttgcgcgc tccatgacgc cggtggtgaa atattgggtc tgcaagattg cgcctgcgct cctttatgaa gcgatggagt gccttggcgg caacggctat atcgaagatg gcaatctggc gcgggcctat cgtgaagcgc cggtcaatgc gatatgggaa ggttccggca atgtgatggc gctggacgtg gcacgggtgc tctcgcgtgc gccggcactt ttcgatggcg tgctggactg gatcagcgga caattgggcc cgcgcgggca gggcacgatc gacgtgctgc gcgccgccct tcaactgacc gagacggatc agggtgtggc acgtcttttg accgagcagc ttgccttcgc agccgcagcc gctgaactgc gccagcttgg cgcggacgat attgctgatg cctttatcga aacccgcctc ggcggcctgt ggcgcaccac ctacgggatg ctggatgcgc gccacaatgc gatgcgtatc atcgaccagc tctatccggc gtcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrmeA.01048.a.A1.PS01389
| PURIFICATION DATe: | 2/21/2012 |
| CONCENTRATION: | 22.54mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MLNQAKRNEP LKTHEVTNQT PPITGTNAYL GDPLLMQIAA RFPKELHTEL EQAGRFVLSA EAQDLARLAN TELPKLRTHD RQGRRIDLVE YHPAYHALMR RSVAQGLHSS IWEDNPLESG RRHQARAARF YLTAQLEAGH LCPLTMTSAS LAALMASPEV YKQWSPAVLS RKYDFSQKPA FRKQGVTLGM GMTEKQGGTD VRANATRAEP AIGGAWRLTG HKWFMSAPMS DAFLTLAQTK XGLSCFLLPR LGEKGESNGF FFQRLKDKLG NRSNASSEVE FDGALGQMIG SPGEGVKTXM DMVTXTRLDC AVASAGLMRS GLAEAVHHSR HRHVFGKPLV EQPLMQRVLA DMALDVAGAT ALSMRLARAF DMAASDRAEA AFARSMTPVV KYWVCKIAPA LLYEAMECLG GNGYIEDGNL ARAYREAPVN AIWEGSGNVM ALDVARVLSR APALFDGVLD WISGQLGPRG QGTIDVLRAA LQLTETDQGV ARLLTEQLAF AAAAAELRQL GADDIADAFI ETRLGGLWRT TYGMLDARHN AMRIIDQLYP AS
Validated NT Sequence
acgacgccgg atagagctgg tcgatgatac gcatcgcatt gtggcgcgca tccagcatcc cgtaggtggt gcgccacagg ccgccgaggc gggtttcgat aaaggcatca gcaatatcgt ccgcgccaag ctggcgcagt tcagcggctg cggctgcgaa ggcaagctgc tcggtcaaaa gacgtgccac accctgatcc gtctcggtca gttgaagggc ggcgcgcagc acgtcgatcg tgccctgccc gcgcgggccc aattgtccgc tgatccagtc cagcacgcca tcgaaaagtg ccggcgcacg cgagagcacc cgtgccacgt ccagcgccat cacattgccg gaaccttccc atatcgcatt gaccggcgct tcacgatagg cccgcgccag attgccatct tcgatatagc cgttgccgcc aaggcactcc atcgcttcat aaaggagcgc aggcgcaatc ttgcagaccc aatatttcac caccggcgtc atggagcgcg caaaagcggc ttccgcccgg tcgcttgccg ccatgtcgaa ngcgcgggca agacgcatgg aaagcgccgt ggcgcccgcc acgtccagcg ccatatcggc cagaacgcgc tgcatcagcg gctgctcgac caaaggcttg ccgaacacat ggcggtggcg gctgtggtga acggcctcag ccagccctga gcgcataagt ccggcagaag ctaccgcgca atcaagccgc gtcnacgtca ccatatccat gancgtcttc acgccctcgc ccggactgcc gatcatctgg cccaaagccc cgtcaaattc cacttccgag gaagcattgg atctgttgcc gagcttatct ttcaggcgct ggaagaaaaa gccattgctc tcgccctttt cgcccagtcg cggcagaagg aaacaggaaa gcccntcctt cgtttgcgcc agtgtcagaa aagcatctga catcggcgcc gacatgaacc atttatggcc cgtaagccgc catgccccgc caatggcagg ttcggcacgg gtggcgttgg cgcgaacatc ggtgccgccc tgtttttccg tcatgcccat gccaagcgta acaccctgtt tgcggaaggc aggcttttgc gaaaaatcat atttgcgcga caggactgcc ggagaccatt gcttgtaaac ctccggcgag gccatgagtg cggccagaga ggcgctcgtc atggtgagcg ggcaaaggtg cccggcctca agctgcgcag tgagataaaa acgggcggca cgcgcctgat gacggcggcc actctccagc ggattgtctt cccatatgga cgaatgcagc ccctgcgcca cggagcggcg catcagcgcg tgataggccg ggtgatattc gacaaggtcg atgcggcggc cctgccggtc atgcgtacgc agcttcggca attcggtgtt ggcaagacga gccaaatcct gcgcctcggc cgaaagtacg aaacggcccg cctgctccag ttctgtatga agctcttttg gaaaacgcgc cgcaatctgc atgagaagcg ggtcgcccag ataagcgttc gtgccggtga tcggcggcgt ctggttggtg acttcgtgcg ttttcaaggg ttcgttccgt ttggcttgat tcaacatcga accaggaccc tgggtctgag cttccagggt a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQAKRNE PLKTHEVTNQ TPPITGTNAY LGDPLLMQIA ARFPKELHTE LEQAGRFVLS AEAQDLARLA NTELPKLRTH DRQGRRIDLV EYHPAYHALM RRSVAQGLHS SIWEDNPLES GRRHQARAAR FYLTAQLEAG HLCPLTMTSA SLAALMASPE VYKQWSPAVL SRKYDFSQKP AFRKQGVTLG MGMTEKQGGT DVRANATRAE PAIGGAWRLT GHKWFMSAPM SDAFLTLAQT KEGLSCFLLP RLGEKGESNG FFFQRLKDKL GNRSNASSEV EFDGALGQMI GSPGEGVKTI MDMVTLTRLD CAVASAGLMR SGLAEAVHHS RHRHVFGKPL VEQPLMQRVL ADMALDVAGA TALSMRLARA FDMAASDRAE AAFARSMTPV VKYWVCKIAP ALLYEAMECL GGNGYIEDGN LARAYREAPV NAIWEGSGNV MALDVARVLS RAPALFDGVL DWISGQLGPR GQGTIDVLRA ALQLTETDQG VARLLTEQLA FAAAAAELRQ LGADDIADAF IETRLGGLWR TTYGMLDARH NAMRIIDQLY PAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGAATCAA GCCAAACGGA ACGAACCCTT GAAAACGCAC GAAGTCACCA ACCAGACGCC GCCGATCACC GGCACGAACG CTTATCTGGG CGACCCGCTT CTCATGCAGA TTGCGGCGCG TTTTCCAAAA GAGCTTCATA CAGAACTGGA GCAGGCGGGC CGTTTCGTAC TTTCGGCCGA GGCGCAGGAT TTGGCTCGTC TTGCCAACAC CGAATTGCCG AAGCTGCGTA CGCATGACCG GCAGGGCCGC CGCATCGACC TTGTCGAATA TCACCCGGCC TATCACGCGC TGATGCGCCG CTCCGTGGCG CAGGGGCTGC ATTCGTCCAT ATGGGAAGAC AATCCGCTGG AGAGTGGCCG CCGTCATCAG GCGCGTGCCG CCCGTTTTTA TCTCACTGCG CAGCTTGAGG CCGGGCACCT TTGCCCGCTC ACCATGACGA GCGCCTCTCT GGCCGCACTC ATGGCCTCGC CGGAGGTTTA CAAGCAATGG TCTCCGGCAG TCCTGTCGCG CAAATATGAT TTTTCGCAAA AGCCTGCCTT CCGCAAACAG GGTGTTACGC TTGGCATGGG CATGACGGAA AAACAGGGCG GCACCGATGT TCGCGCCAAC GCCACCCGTG CCGAACCTGC CATTGGCGGG GCATGGCGGC TTACGGGCCA TAAATGGTTC ATGTCGGCGC CGATGTCAGA TGCTTTTCTG ACACTGGCGC AAACGAAGGA GGGGCTTTCC TGTTTCCTTC TGCCGCGACT GGGCGAAAAG GGCGAGAGCA ATGGCTTTTT CTTCCAGCGC CTGAAAGATA AGCTCGGCAA CAGATCCAAT GCTTCCTCGG AAGTGGAATT TGACGGGGCT TTGGGCCAGA TGATCGGCAG TCCGGGCGAG GGCGTGAAGA CGATCATGGA TATGGTGACG TTGACGCGGC TTGATTGCGC GGTAGCTTCT GCCGGACTTA TGCGCTCAGG GCTGGCTGAG GCCGTTCACC ACAGCCGCCA CCGCCATGTG TTCGGCAAGC CTTTGGTCGA GCAGCCGCTG ATGCAGCGCG TTCTGGCCGA TATGGCGCTG GACGTGGCGG GCGCCACGGC GCTTTCCATG CGTCTTGCCC GCGCCTTCGA CATGGCGGCA AGCGACCGGG CGGAAGCCGC TTTTGCGCGC TCCATGACGC CGGTGGTGAA ATATTGGGTC TGCAAGATTG CGCCTGCGCT CCTTTATGAA GCGATGGAGT GCCTTGGCGG CAACGGCTAT ATCGAAGATG GCAATCTGGC GCGGGCCTAT CGTGAAGCGC CGGTCAATGC GATATGGGAA GGTTCCGGCA ATGTGATGGC GCTGGACGTG GCACGGGTGC TCTCGCGTGC GCCGGCACTT TTCGATGGCG TGCTGGACTG GATCAGCGGA CAATTGGGCC CGCGCGGGCA GGGCACGATC GACGTGCTGC GCGCCGCCCT TCAACTGACC GAGACGGATC AGGGTGTGGC ACGTCTTTTG ACCGAGCAGC TTGCCTTCGC AGCCGCAGCC GCTGAACTGC GCCAGCTTGG CGCGGACGAT ATTGCTGATG CCTTTATCGA AACCCGCCTC GGCGGCCTGT GGCGCACCAC CTACGGGATG CTGGATGCGC GCCACAATGC GATGCGTATC ATCGACCAGC TCTATCCGGC GTCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA