SSGCID: Target Page

BrmeA.00808.a

L-asparaginase (EC 3.5.1.1)

CENTER ID:	BrmeA.00808.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00808.a.B1.GE36686	Full length( BrmeA.00808.a )		1	314

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel92729_0108
BV-BRC:	fig\|224914.11.peg.108
RefSeq:	NP_539023.1
UniProt:	Q8YJH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLISIGTLG GTIAMVEGGS GGGVVPTLTA DALIAAVPQI RNYAQIKAQS LFQLPSPSLK FRHGLEVIGW AENEVKSGAD GIVLTQGTDT LEEMAFLLDL LWEHPQPLVL TGAMRSPRAP GADGPANLVA AVLTAASRLS RERGVLVAMN DTIHAARWIT KSHALSVQTF VSSDSGPLGY VYEETPVYIN TLSRLPKIDR TQFNADVKIG FYESLLDGDA QMLRSMFESG RYDGIVVAGF GAGHVSGDEA DIIERYASRI PVVVASRTYG GRTATKTYGF HGSEIDLQTK GAMLAGWLSP PQGAVIAMGT TGSG

NT Sequence

ATGTCTTTAA TTTCTATCGG CACCCTCGGG GGTACGATTG CGATGGTTGA GGGGGGAAGC GGAGGCGGTG TTGTGCCGAC CCTGACCGCG GATGCACTCA TTGCCGCTGT GCCGCAGATT AGAAATTATG CGCAGATCAA GGCCCAATCT CTTTTTCAGC TTCCCAGCCC CTCGCTAAAA TTTCGGCACG GCCTTGAAGT GATAGGCTGG GCTGAAAACG AGGTGAAATC GGGAGCGGAC GGCATAGTGC TGACGCAGGG AACCGATACG CTTGAGGAAA TGGCATTCCT TCTGGATTTA TTGTGGGAAC ATCCGCAGCC CCTCGTACTG ACCGGCGCCA TGCGCTCGCC GCGCGCGCCG GGTGCTGACG GTCCGGCCAA TCTGGTGGCG GCCGTATTGA CAGCCGCGAG CAGGCTTAGC CGGGAACGCG GTGTCCTTGT TGCAATGAAC GATACAATCC ACGCGGCACG TTGGATAACA AAATCTCATG CGCTCAGTGT CCAGACATTC GTATCGTCGG ACAGCGGGCC GTTGGGATAT GTGTATGAGG AGACGCCGGT TTACATCAAC ACCCTTTCGC GGTTGCCAAA AATTGACCGT ACGCAGTTCA ACGCCGATGT GAAGATCGGC TTTTACGAAT CGCTGCTGGA TGGTGACGCG CAGATGCTGC GAAGCATGTT TGAGAGCGGC AGATATGATG GAATCGTCGT CGCCGGTTTT GGAGCAGGAC ATGTGTCGGG CGACGAAGCC GACATTATCG AGCGCTATGC GTCCCGCATT CCGGTCGTCG TAGCCAGCCG GACCTATGGC GGAAGGACCG CCACGAAGAC TTATGGGTTC CATGGCTCCG AGATTGATCT TCAGACGAAA GGCGCTATGC TCGCAGGCTG GCTATCCCCC CCGCAAGGCG CGGTTATTGC TATGGGCACT ACTGGCAGCG GG

Details for BrmeA.00808.a.B1.GE36686

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	88

Validated AA Sequence

MAHHHHHHMS LISIGTLGGT IAMVEGGSGG GVVPTLTADA LIAAVPQIRN YAQIKAQSLF QLPSPSLKFR HGLEVIGWAE NEVKSGADGI VLTQGTDTLE EMAFLLDLLW EHPQPLVLTG AMRSPRAPGA DGPANLVAAV LTAASRLSRE RGVLVAMNDT IHAARWITKS HALSVQTFVS SDSGPLGYVY EETPVYINTL SRLPKIDRTQ FNADVKIGFY ESLLDGDAQM LRSMFESGRY DGIVVAGFGA TCVGRRSRHY RALCVPHSGR RXXPXLWRKD RHEDLWVPWL XX

Validated NT Sequence

tnnnnnnnnt tnnttacttt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgtctttaa tttctatcgg caccctcggg ggtacgattg cgatggttga ggggggaagc ggaggcggtg ttgtgccgac cctgaccgcg gatgcactca ttgccgctgt gccgcagatt agaaattatg cgcagatcaa ggcccaatct ctttttcagc ttcccagccc ctcgctaaaa tttcggcacg gccttgaagt gataggctgg gctgaaaacg aggtgaaatc gggagcggac ggcatagtgc tgacgcaggg aaccgatacg cttgaggaaa tggcattcct tctggattta ttgtgggaac atccgcagcc cctcgtactg accggcgcca tgcgctcgcc gcgcgcgccg ggtgctgacg gtccggccaa tctggtggcg gccgtattga cagccgcgag caggcttagc cgggaacgcg gtgtccttgt tgcaatgaac gatacaatcc acgcggcacg ttggataaca aaatctcatg cgctcagtgt ccagacattc gtatcgtcgg acagcgggcc gttgggatat gtgtatgagg agacgccggt ttacatcaac accctttcgc ggttgccaaa aattgaccgt acgcagttca acgccgatgt gaagatcggc ttttacgaat cgctgctgga tggtgacgcg cagatgctgc gaagcatgtt tgagagcggc agatatgatg gaatcgtcgt cgccggtttt ggagcnacat gtgtcgggcg acgaagccga cattatcgag cgctatgcgt cccgcattcc ggtcgtcgta nccngccgga nctatggcgg aaggaccgcc acgaagactt atgggttcca tggctncnan attgatcttc anannaaagg nnctatgctc gcnnntggct atcccccccn gcaaggngcg gtnnntnnta tgggnntact ggcancgggt aantgannaa nana

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt taatttctat cggcaccctc gggggtacga ttgcgatggt tgagggggga agcggaggcg gtgttgtgcc gaccctgacc gcggatgcac tcattgccgc tgtgccgcag attagaaatt atgcgcagat caaggcccaa tctctttttc agcttcccag cccctcgcta aaatttcggc acggccttga agtgataggc tgggctgaaa acgaggtgaa atcgggagcg gacggcatag tgctgacgca gggaaccgat acgcttgagg aaatggcatt ccttctggat ttattgtggg aacatccgca gcccctcgta ctgaccggcg ccatgcgctc gccgcgcgcg ccgggtgctg acggtccggc caatctggtg gcggccgtat tgacagccgc gagcaggctt agccgggaac gcggtgtcct tgttgcaatg aacgatacaa tccacgcggc acgttggata acaaaatctc atgcgctcag tgtccagaca ttcgtatcgt cggacagcgg gccgttggga tatgtgtatg aggagacgcc ggtttacatc aacacccttt cgcggttgcc aaaaattgac cgtacgcagt tcaacgccga tgtgaagatc ggcttttacg aatcgctgct ggatggtgac gcgcagatgc tgcgaagcat gtttgagagc ggcagatatg atggaatcgt cgtcgccggt tttggagcag gacatgtgtc gggcgacgaa gccgacatta tcgagcgcta tgcgtcccgc attccggtcg tcgtagccag ccggacctat ggcggaagga ccgccacgaa gacttatggg ttccatggct ccgagattga tcttcagacg aaaggcgcta tgctcgcagg ctggctatcc cccccgcaag gcgcggttat tgctatgggc actactggca gcgggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat