BrmeA.00771.a

Diaminopimelate decarboxylase (EC 4.1.1.20)

CENTER ID: BrmeA.00771.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrmeA.00771.a.A1.GE33719 full length 1 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrmeA.00771.a.A1.PS01273 full length 1 421
Structures
3VAB
DEPOSITED: 12/29/2011
DETERMINATION: XRay
CLONE: BrmeA.00771.a.A1.GE33719
PROTEIN: BrmeA.00771.a.A1.PS01273
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126750
PATRIC ID: fig|224914.11.peg.86
RefSeq: NP_539002.1
UniProt: Q8YJJ9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHFEYRNGV LHAENVSLPE IAKAVGTPFY VYSRATIERH FRVFHDAFAD MDTLVTYALK ANSNQAVLTA LAKLGAGADT VSQGEIRRAL AAGIPANRIV FSGVGKTPRE MDFALEAGIY CFNVESEPEL EILSARAVAA GKVAPVSLRI NPDVDAKTHA KISTGKSENK FGIPRDKARA AYARAASLPG LNVVGIDMHI GSQIIDLEPF DNAFALMAEL VKELQADGHN IRHVDVGGGL GIPYRTPNTP PPPPVAYAQI VAKHIKPLGL KTVFEPGRLI VGNAGLLVTE VIFVKEGDAK NFVIVDAAMN DLIRPTLYDA FHDIRPVIMP NDNAPRIRAD FVGPVCETGD YLGLDREVAK PAPGDLIAIC TTGAYGAVLS STYNSRLLIP EVLGDGERYH VVRPRRTYEE LLALDSVPDW L
NT Sequence
gtgaaccact ttgaatatcg caacggcgtt ctccacgctg aaaatgtcag cctgcccgaa atagccaagg ccgtcggcac gcctttctat gtctattcgc gcgcaaccat cgagcgccat tttcgcgtgt tccatgacgc ctttgccgat atggacacgc tcgtcaccta tgcgctgaag gcgaattcca accaggctgt gctgacggcg ctggcaaagc ttggcgcggg cgcggacacg gtttcgcagg gcgagatccg ccgtgcgctt gccgcgggca ttccggcaaa caggattgta ttttccggcg tcggcaagac cccgcgcgaa atggattttg cgctggaagc tggcatttac tgtttcaacg tcgaatccga gccggagctg gaaattcttt cagcccgcgc cgtcgccgcc ggcaaggtgg cgcctgtctc gctgcgtatc aacccggatg tggatgcgaa gacccacgcg aaaatctcca ccggcaagtc ggagaacaag ttcggcatcc cgcgcgacaa ggcgcgcgcg gcctatgccc gcgcggcaag cctgcccggc ctcaacgtcg tcggcatcga catgcacatc ggaagccaga tcatcgatct tgagcctttc gacaatgctt tcgcgctgat ggcggaactg gtgaaggaat tgcaggccga tggccacaat atccgccatg tcgatgtggg cggcgggctg ggcattccct accgcacccc caacacgccg ccgccgccac ctgttgccta tgcccaaata gtggcgaaac acatcaagcc gctcggcctc aagaccgtgt ttgagccggg ccgcctgatc gtcggcaatg ccgggcttct cgtgacggaa gtaattttcg tgaaggaagg cgatgcgaag aatttcgtga tcgtggacgc ggcgatgaac gatctcatcc gcccgacgct ttacgatgcc tttcacgata tcaggccggt cattatgccg aacgacaatg ccccgcgcat ccgcgccgat ttcgtcggtc cggtctgcga gacgggcgac tatctgggcc tcgaccgcga ggtggcaaag cccgcccccg gcgacctgat cgccatctgc accaccggcg cttatggcgc ggtgctttcc agcacctata acagccgcct tctcattccc gaagtgctgg gggacggcga gcgttaccat gtggttcgtc cgcgccgcac ctatgaggaa ttgctggcgc tcgattccgt gccggactgg ctgtaa
Details for BrmeA.00771.a.A1.GE33719
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHFEYRN GVLHAENVSL PEIAKAVGTP FYVYSRATIE RHFRVFHDAF ADMDTLVTYA LKANSNQAVL TALAKLGAGA DTVSQGEIRR ALAAGIPANR IVFSGVGKTP REMDFALEAG IYCFNVESEP ELEILSARAV AAGKVAPVSL RINPDVDAKT HAKISTGKSE NKFGIPRDKA RAAYARAASL PGLNVVGIDM HIGSQIIDLE PFDNAFALMA ELVKELQADG HNIRHVDVGG GLGIPYRTDN TPPPPPVAYA QIVAKHIKPL GLKXVFEPGR LIVGNAGLLV TEVIFVXXXX AKNFXIXXAA XNDLIXRXXX XXXXIXXXXX TXXPRIXXFX XXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
ttgtttnntt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgaacc actttgaata tcgcaacggc gttctccacg ctgaaaatgt cagcctgccc gaaatagcca aggccgtcgg cacgcctttc tatgtctatt cgcgcgcaac catcgagcgc cattttcgcg tgttccatga cgcctttgcc gatatggaca cgctcgtcac ctatgcgctg aaggcgaatt ccaaccaggc tgtgctgacg gcgctggcaa agcttggcgc gggcgcggac acggtttcgc agggcgagat ccgccgtgcg cttgccgcgg gcattccggc aaacaggatt gtattttccg gcgtcggcaa gaccccgcgc gaaatggatt ttgcgctgga agctggcatt tactgtttca acgtcgaatc cgagccggag ctggaaattc tttcagcccg cgccgtcgcc gccggcaagg tggcgcctgt ctcgctgcgt atcaacccgg atgtggatgc gaagacccac gcgaaaatct ccaccggcaa gtcggagaac aagttcggca tcccgcgcga caaggcgcgc gcggcctatg cccgcgcggc aagcctgccc ggcctcaacg tcgtcggcat cgacatgcac atcggaagcc agatcatcga tcttgagcct ttcgacaatg ctttcgcgct gatggcggaa ctggtgaagg aattgcaggc cgatggccac aatatccgcc atgtcgatgt gggcggcggg ctgggcattc cctaccgcac cgacaacacg ccgccgccgc cacctgttgc ctatgcccaa atagtggcga aacacatcaa gccgctcggc ctcaagancg tgtttgagcc gggccgcctg atcgtcggca atgccgggct tctcgtgacg gaagtaattt tcgtgaanna nnnnnangcg aagaatttcg ngatcgnngn ngcngcgatn aacgatctca tcnnccgang cnnncnatnn nttcnnnata tcngnnncat nntncnaacg annnncccgc gcatcnnnnn atttcnnnnn cnnntgnnnn nnggnnantn nnnnnnnnna cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncnnnnnnng nnnnnnnngn nnnggnnnnn ttnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHFEYRN GVLHAENVSL PEIAKAVGTP FYVYSRATIE RHFRVFHDAF ADMDTLVTYA LKANSNQAVL TALAKLGAGA DTVSQGEIRR ALAAGIPANR IVFSGVGKTP REMDFALEAG IYCFNVESEP ELEILSARAV AAGKVAPVSL RINPDVDAKT HAKISTGKSE NKFGIPRDKA RAAYARAASL PGLNVVGIDM HIGSQIIDLE PFDNAFALMA ELVKELQADG HNIRHVDVGG GLGIPYRTPN TPPPPPVAYA QIVAKHIKPL GLKTVFEPGR LIVGNAGLLV TEVIFVKEGD AKNFVIVDAA MNDLIRPTLY DAFHDIRPVI MPNDNAPRIR ADFVGPVCET GDYLGLDREV AKPAPGDLIA ICTTGAYGAV LSSTYNSRLL IPEVLGDGER YHVVRPRRTY EELLALDSVP DWL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaccac tttgaatatc gcaacggcgt tctccacgct gaaaatgtca gcctgcccga aatagccaag gccgtcggca cgcctttcta tgtctattcg cgcgcaacca tcgagcgcca ttttcgcgtg ttccatgacg cctttgccga tatggacacg ctcgtcacct atgcgctgaa ggcgaattcc aaccaggctg tgctgacggc gctggcaaag cttggcgcgg gcgcggacac ggtttcgcag ggcgagatcc gccgtgcgct tgccgcgggc attccggcaa acaggattgt attttccggc gtcggcaaga ccccgcgcga aatggatttt gcgctggaag ctggcattta ctgtttcaac gtcgaatccg agccggagct ggaaattctt tcagcccgcg ccgtcgccgc cggcaaggtg gcgcctgtct cgctgcgtat caacccggat gtggatgcga agacccacgc gaaaatctcc accggcaagt cggagaacaa gttcggcatc ccgcgcgaca aggcgcgcgc ggcctatgcc cgcgcggcaa gcctgcccgg cctcaacgtc gtcggcatcg acatgcacat cggaagccag atcatcgatc ttgagccttt cgacaatgct ttcgcgctga tggcggaact ggtgaaggaa ttgcaggccg atggccacaa tatccgccat gtcgatgtgg gcggcgggct gggcattccc taccgcaccc ccaacacgcc gccgccgcca cctgttgcct atgcccaaat agtggcgaaa cacatcaagc cgctcggcct caagaccgtg tttgagccgg gccgcctgat cgtcggcaat gccgggcttc tcgtgacgga agtaattttc gtgaaggaag gcgatgcgaa gaatttcgtg atcgtggacg cggcgatgaa cgatctcatc cgcccgacgc tttacgatgc ctttcacgat atcaggccgg tcattatgcc gaacgacaat gccccgcgca tccgcgccga tttcgtcggt ccggtctgcg agacgggcga ctatctgggc ctcgaccgcg aggtggcaaa gcccgccccc ggcgacctga tcgccatctg caccaccggc gcttatggcg cggtgctttc cagcacctat aacagccgcc ttctcattcc cgaagtgctg ggggacggcg agcgttacca tgtggttcgt ccgcgccgca cctatgagga attgctggcg ctcgattccg tgccggactg gctgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrmeA.00771.a.A1.PS01273
PURIFICATION DATe: 11/9/2011
CONCENTRATION: 36.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 83
PERCENT COVERAGE: 88
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHFEYRN GVLHAENVSL PEIAKAVGTP FYVYSRATIE RHFRVFHDAF ADMDTLVTYA LKANSNQAVL TALAKLGAGA DTVSQGEIRR ALAAGIPANR IVFSGVGKTP REMDFALEAG IYCFNVESEP ELEILSARAV AAGKVAPVSL RINPDVDAKT HAKISTGKSE NKFGIPRDKA RAAYARAASL PGLNVVGIDM HIGSQIIDLE PFDNAFALMA ELVKELQADG HNIRHVDVGG GLGIPYRTDN TPPPPPVAYA QIVAKHIKPL GLKXVFEPGR LIVGNAGLLV TEVIFVXXXX AKNFXIXXAA XNDLIXRXXX XXXXIXXXXX TXXPRIXXFX XXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
ttgtttnntt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgaacc actttgaata tcgcaacggc gttctccacg ctgaaaatgt cagcctgccc gaaatagcca aggccgtcgg cacgcctttc tatgtctatt cgcgcgcaac catcgagcgc cattttcgcg tgttccatga cgcctttgcc gatatggaca cgctcgtcac ctatgcgctg aaggcgaatt ccaaccaggc tgtgctgacg gcgctggcaa agcttggcgc gggcgcggac acggtttcgc agggcgagat ccgccgtgcg cttgccgcgg gcattccggc aaacaggatt gtattttccg gcgtcggcaa gaccccgcgc gaaatggatt ttgcgctgga agctggcatt tactgtttca acgtcgaatc cgagccggag ctggaaattc tttcagcccg cgccgtcgcc gccggcaagg tggcgcctgt ctcgctgcgt atcaacccgg atgtggatgc gaagacccac gcgaaaatct ccaccggcaa gtcggagaac aagttcggca tcccgcgcga caaggcgcgc gcggcctatg cccgcgcggc aagcctgccc ggcctcaacg tcgtcggcat cgacatgcac atcggaagcc agatcatcga tcttgagcct ttcgacaatg ctttcgcgct gatggcggaa ctggtgaagg aattgcaggc cgatggccac aatatccgcc atgtcgatgt gggcggcggg ctgggcattc cctaccgcac cgacaacacg ccgccgccgc cacctgttgc ctatgcccaa atagtggcga aacacatcaa gccgctcggc ctcaagancg tgtttgagcc gggccgcctg atcgtcggca atgccgggct tctcgtgacg gaagtaattt tcgtgaanna nnnnnangcg aagaatttcg ngatcgnngn ngcngcgatn aacgatctca tcnnccgang cnnncnatnn nttcnnnata tcngnnncat nntncnaacg annnncccgc gcatcnnnnn atttcnnnnn cnnntgnnnn nnggnnantn nnnnnnnnna cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncnnnnnnng nnnnnnnngn nnnggnnnnn ttnnnnnnnn c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHFEYRN GVLHAENVSL PEIAKAVGTP FYVYSRATIE RHFRVFHDAF ADMDTLVTYA LKANSNQAVL TALAKLGAGA DTVSQGEIRR ALAAGIPANR IVFSGVGKTP REMDFALEAG IYCFNVESEP ELEILSARAV AAGKVAPVSL RINPDVDAKT HAKISTGKSE NKFGIPRDKA RAAYARAASL PGLNVVGIDM HIGSQIIDLE PFDNAFALMA ELVKELQADG HNIRHVDVGG GLGIPYRTPN TPPPPPVAYA QIVAKHIKPL GLKTVFEPGR LIVGNAGLLV TEVIFVKEGD AKNFVIVDAA MNDLIRPTLY DAFHDIRPVI MPNDNAPRIR ADFVGPVCET GDYLGLDREV AKPAPGDLIA ICTTGAYGAV LSSTYNSRLL IPEVLGDGER YHVVRPRRTY EELLALDSVP DWL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAACCAC TTTGAATATC GCAACGGCGT TCTCCACGCT GAAAATGTCA GCCTGCCCGA AATAGCCAAG GCCGTCGGCA CGCCTTTCTA TGTCTATTCG CGCGCAACCA TCGAGCGCCA TTTTCGCGTG TTCCATGACG CCTTTGCCGA TATGGACACG CTCGTCACCT ATGCGCTGAA GGCGAATTCC AACCAGGCTG TGCTGACGGC GCTGGCAAAG CTTGGCGCGG GCGCGGACAC GGTTTCGCAG GGCGAGATCC GCCGTGCGCT TGCCGCGGGC ATTCCGGCAA ACAGGATTGT ATTTTCCGGC GTCGGCAAGA CCCCGCGCGA AATGGATTTT GCGCTGGAAG CTGGCATTTA CTGTTTCAAC GTCGAATCCG AGCCGGAGCT GGAAATTCTT TCAGCCCGCG CCGTCGCCGC CGGCAAGGTG GCGCCTGTCT CGCTGCGTAT CAACCCGGAT GTGGATGCGA AGACCCACGC GAAAATCTCC ACCGGCAAGT CGGAGAACAA GTTCGGCATC CCGCGCGACA AGGCGCGCGC GGCCTATGCC CGCGCGGCAA GCCTGCCCGG CCTCAACGTC GTCGGCATCG ACATGCACAT CGGAAGCCAG ATCATCGATC TTGAGCCTTT CGACAATGCT TTCGCGCTGA TGGCGGAACT GGTGAAGGAA TTGCAGGCCG ATGGCCACAA TATCCGCCAT GTCGATGTGG GCGGCGGGCT GGGCATTCCC TACCGCACCC CCAACACGCC GCCGCCGCCA CCTGTTGCCT ATGCCCAAAT AGTGGCGAAA CACATCAAGC CGCTCGGCCT CAAGACCGTG TTTGAGCCGG GCCGCCTGAT CGTCGGCAAT GCCGGGCTTC TCGTGACGGA AGTAATTTTC GTGAAGGAAG GCGATGCGAA GAATTTCGTG ATCGTGGACG CGGCGATGAA CGATCTCATC CGCCCGACGC TTTACGATGC CTTTCACGAT ATCAGGCCGG TCATTATGCC GAACGACAAT GCCCCGCGCA TCCGCGCCGA TTTCGTCGGT CCGGTCTGCG AGACGGGCGA CTATCTGGGC CTCGACCGCG AGGTGGCAAA GCCCGCCCCC GGCGACCTGA TCGCCATCTG CACCACCGGC GCTTATGGCG CGGTGCTTTC CAGCACCTAT AACAGCCGCC TTCTCATTCC CGAAGTGCTG GGGGACGGCG AGCGTTACCA TGTGGTTCGT CCGCGCCGCA CCTATGAGGA ATTGCTGGCG CTCGATTCCG TGCCGGACTG GCTGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA