BrmeA.00629.a

Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases

CENTER ID: BrmeA.00629.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00629.a.B1.GE36684 Full length( BrmeA.00629.a ) 1 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_3154
BV-BRC: fig|224914.11.peg.3154
RefSeq: NP_541793.1
RefSeq: WP_011005714.1
UniProt: Q8YBS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTESPASARL DRYDDAVAQF DIAKAIALLA GNPDTGINAC IECCDRYTGE NRVALHAISI DNTLTELTFE DLRDMSARVG NMLADAGISA GDVVAGLLPR TPELVATILG AWRIGAIYQP LFTAFGPKAI EQRFGTSGAK LVVTNLANRS KLAEVENCPR VATILAPGES LPEGDIDFRA AVAAASTECE PVMRKGSDLF MMMSTSGTAG LPKGVPVPLR ALMAFGAYMR DAWAAQRRYF WNIADPGWAY GLYYAVTGPL LLGVPTILNE GGFTAENTYD IIERLGVTSL AGSPTAFRLL MAAGPESAAR VKGRLRVASS AGEPLNPEVI RWFDACLGAP IHDHYGQTEL GMVVNNHHGL EHPVRQGSAG YAMPGYRVAV LDEAGKEVGP NEPGVLAIDI DNSPLLWFTG YYKKDTPSIS GGYYRTGDTV EFEPHGSISF IGRADDVITS SGYRIGPFDV ESALLEHPAV NEAAVVGVPD PQRTEIVKAF VILAPGFEGT PELAEELALH VKKQLSAHAY PRQIDFVAEL PKTPSGKIQR FLLRKAEVEK QQQQN
NT Sequence
ATGACTGAAA GCCCTGCTTC AGCACGGCTC GACCGCTACG ATGATGCCGT TGCGCAGTTC GATATTGCAA AAGCCATTGC GCTTCTTGCA GGAAATCCTG ATACGGGTAT CAATGCCTGC ATTGAATGCT GCGACCGATA TACGGGCGAA AATCGCGTGG CGCTGCACGC CATTTCCATC GACAATACGT TGACTGAACT CACCTTTGAA GACCTGCGCG ATATGTCCGC GCGGGTCGGC AATATGCTGG CCGATGCCGG GATCAGCGCA GGCGATGTGG TTGCCGGTCT TCTCCCCCGC ACGCCTGAAC TCGTCGCCAC CATTCTCGGC GCCTGGCGCA TCGGCGCAAT CTATCAGCCG CTTTTCACCG CCTTCGGCCC AAAAGCCATC GAACAGCGTT TCGGCACCAG TGGCGCCAAA CTCGTCGTCA CCAACCTGGC CAATCGTTCC AAATTGGCCG AAGTGGAAAA CTGCCCCCGC GTCGCAACCA TTCTTGCCCC CGGTGAAAGC CTGCCGGAAG GCGATATCGA CTTTCGCGCA GCCGTGGCGG CAGCATCGAC CGAATGCGAG CCCGTCATGC GCAAGGGCAG CGACCTTTTC ATGATGATGT CCACCTCCGG CACGGCGGGC CTGCCCAAAG GCGTCCCGGT GCCGCTGCGC GCCCTGATGG CTTTCGGCGC CTATATGCGC GATGCGTGGG CTGCGCAGCG ACGATATTTC TGGAATATCG CCGATCCGGG CTGGGCTTAT GGGCTTTACT ATGCCGTGAC GGGTCCCCTG CTTCTGGGTG TGCCGACGAT CCTGAACGAA GGCGGTTTCA CCGCCGAAAA CACCTATGAC ATCATCGAAC GCCTCGGCGT GACGAGCCTT GCGGGCTCTC CGACTGCCTT CCGCCTGCTT ATGGCCGCCG GGCCGGAATC TGCTGCCCGC GTCAAGGGCC GCTTGCGCGT GGCATCCAGC GCGGGCGAGC CGCTGAACCC GGAAGTCATT CGCTGGTTCG ATGCCTGTCT TGGCGCGCCG ATCCACGATC ATTACGGCCA GACGGAACTG GGCATGGTGG TGAACAACCA TCATGGATTG GAACATCCGG TGCGGCAGGG TTCCGCGGGC TATGCCATGC CGGGCTACAG GGTTGCGGTT CTGGATGAAG CGGGCAAAGA AGTCGGCCCC AATGAACCGG GCGTTCTGGC CATTGATATC GACAATTCGC CGCTTCTCTG GTTCACCGGC TATTATAAAA AGGATACGCC CTCCATCTCC GGCGGATATT ATCGCACCGG CGATACGGTG GAATTCGAGC CGCATGGGTC TATCAGCTTC ATCGGCCGCG CCGATGACGT CATCACCTCC TCCGGCTATC GCATCGGGCC TTTCGATGTG GAAAGCGCGC TTCTGGAACA TCCTGCGGTC AACGAGGCTG CGGTGGTCGG CGTTCCCGAT CCGCAACGCA CCGAAATCGT GAAGGCCTTT GTCATCCTGG CCCCCGGCTT CGAGGGTACG CCGGAACTGG CGGAAGAACT GGCGCTTCAC GTCAAGAAAC AGCTTTCCGC CCATGCCTAT CCGCGGCAGA TCGACTTTGT GGCGGAGCTT CCCAAGACGC CGAGCGGAAA AATACAGCGC TTCCTCCTGC GCAAGGCGGA AGTCGAAAAG CAACAGCAAC AGAAT
Details for BrmeA.00629.a.B1.GE36684
HARVESTED ON: data unavailable
SEQUENCED ON: 2/2/2013
EXPECTED MW: 62kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT ESPASARLDR YDDAVAQFDI AKAIALLAGN PDTGINACIE CCDRYTGENR VALHAISIDN TLTELTFEDL RDMSARVGNM LADAGISAGD VVAGLLPRTP ELVATILGAW RIGAIYQPLF TAFGPKAIEQ RFGTSGAKLV VTNLANRSKL AEVENCPRVA TILAPGESLP EGDIDFRAAV AAASTECEPV MRKGSDLFMM MSTSGTAGLP KGVPVPLRAL MAFGAYMRDA WAAQRRYFWN IADPGWAYGL YYAVTGPLLL GVPTILNEGG FTAENTYDII ERLGVTSLAG SPTAFRLLMA AGPESAARVK GRLRVASSAG EPLNPEVIRW FDACLGAPIH DHYGQTELGM VVNNHHGLEH PVRQGSAGYA MPGYRVAVLD EAGKEVGPNE PGVLAIDIDN SPLLWFTGYY KKDTPSISGG YYRTGDTVEF EPHGSISFIG RADDVITSSG YRIGPFDVES ALLEHPAVNE AAVVGVPDPQ RTEIVKAFVI LAPGFEGTPE LAEELALHVK KQLSAHAYPR QIDFVAELPK TPSGKIQRFL LRKAEVEKQQ QQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aaagccctgc ttcagcacgg ctcgaccgct acgatgatgc cgttgcgcag ttcgatattg caaaagccat tgcgcttctt gcaggaaatc ctgatacggg tatcaatgcc tgcattgaat gctgcgaccg atatacgggc gaaaatcgcg tggcgctgca cgccatttcc atcgacaata cgttgactga actcaccttt gaagacctgc gcgatatgtc cgcgcgggtc ggcaatatgc tggccgatgc cgggatcagc gcaggcgatg tggttgccgg tcttctcccc cgcacgcctg aactcgtcgc caccattctc ggcgcctggc gcatcggcgc aatctatcag ccgcttttca ccgccttcgg cccaaaagcc atcgaacagc gtttcggcac cagtggcgcc aaactcgtcg tcaccaacct ggccaatcgt tccaaattgg ccgaagtgga aaactgcccc cgcgtcgcaa ccattcttgc ccccggtgaa agcctgccgg aaggcgatat cgactttcgc gcagccgtgg cggcagcatc gaccgaatgc gagcccgtca tgcgcaaggg cagcgacctt ttcatgatga tgtccacctc cggcacggcg ggcctgccca aaggcgtccc ggtgccgctg cgcgccctga tggctttcgg cgcctatatg cgcgatgcgt gggctgcgca gcgacgatat ttctggaata tcgccgatcc gggctgggct tatgggcttt actatgccgt gacgggtccc ctgcttctgg gtgtgccgac gatcctgaac gaaggcggtt tcaccgccga aaacacctat gacatcatcg aacgcctcgg cgtgacgagc cttgcgggct ctccgactgc cttccgcctg cttatggccg ccgggccgga atctgctgcc cgcgtcaagg gccgcttgcg cgtggcatcc agcgcgggcg agccgctgaa cccggaagtc attcgctggt tcgatgcctg tcttggcgcg ccgatccacg atcattacgg ccagacggaa ctgggcatgg tggtgaacaa ccatcatgga ttggaacatc cggtgcggca gggttccgcg ggctatgcca tgccgggcta cagggttgcg gttctggatg aagcgggcaa agaagtcggc cccaatgaac cgggcgttct ggccattgat atcgacaatt cgccgcttct ctggttcacc ggctattata aaaaggatac gccctccatc tccggcggat attatcgcac cggcgatacg gtggaattcg agccgcatgg gtctatcagc ttcatcggcc gcgccgatga cgtcatcacc tcctccggct atcgcatcgg gcctttcgat gtggaaagcg cgcttctgga acatcctgcg gtcaacgagg ctgcggtggt cggcgttccc gatccgcaac gcaccgaaat cgtgaaggcc tttgtcatcc tggcccccgg cttcgagggt acgccggaac tggcggaaga actggcgctt cacgtcaaga aacagctttc cgcccatgcc tatccgcggc agatcgactt tgtggcggag cttcccaaga cgccgagcgg aaaaatacag cgcttcctcc tgcgcaaggc ggaagtcgaa aagcaacagc aacagaattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat