SSGCID: Target Page

BrmeA.00587.a

Transcriptional regulatory protein chvi

CENTER ID:	BrmeA.00587.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00587.a.A1.GE33721	full length		1	239

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.2247
RefSeq:	NP_540953.1
UniProt:	Q8YE42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKEASATQTI ALVDDDRNIL TSVSIALESE GYRVETYTDG ASALDGLMAR PPNLAIFDIK MPRMDGMELL RRLRQKSDLP VIFLTSKDDE IDELFGLKMG ADDFITKPFS QRLLVERVKA VLRRVAARDG TAKPAGQQAK SLERGQLVMD QERHTCTWKG EPVTLTVTEF LILHSLAQRP GVVKSRDALM DAAYDEQVYV DDRTIDSHIK RLRKKFKAVD DSFEMIETLY GVGYRFREA

NT Sequence

atgaaggaag cttcggcaac gcagacgatt gcgctggttg atgatgaccg caacatcctg acctccgttt ccattgcgct ggagtcggaa ggttatcgcg tcgaaaccta taccgacggc gcttctgcgc tggatgggtt gatggcgcgt ccgcccaatc ttgcgatctt cgatatcaag atgccgcgca tggacggtat ggagcttctg cgccgcctgc gccagaagtc cgatctgccg gtcatcttcc tcacttccaa ggacgatgag atcgatgaac tcttcggcct caagatgggg gcggacgatt tcatcaccaa gcctttttcg cagcgtcttc tggtcgagcg tgtcaaggct gttctgcgcc gcgtggcggc gcgcgacggc acggcaaagc ccgccggcca gcaggccaag tcccttgagc gcggacagct cgtcatggat caggagcgcc atacctgcac ctggaagggt gagccggtta cgctgactgt taccgaattt ctcatccttc actcacttgc ccagcgcccc ggcgtggtca aaagccgtga cgccctgatg gatgcggcct atgatgaaca ggtctatgtg gatgatcgca ccatcgacag ccacatcaag cgtctgcgca agaagttcaa ggcggttgac gacagcttcg agatgatcga aacgctctac ggcgtcggtt atcgtttccg ggaagcgtaa

Details for BrmeA.00587.a.A1.GE33721

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKEASATQT IALVDDDRNI LTSVSIALES EGYRVETYTD GASALDGLMA RPPNLAIFDI KMPRMDGMEL LRRLRQKSDL PVIFLTSKDD EIDELFGLKM GADDFITKPF SQRLLVERVK AVLRRVAARD GTAKPAGQQA KSLERGQLVM DQERHTCTWK GEPVTLTVTE FLILHSLAQR PGVVKSRDAL MDAAYDEQVY VDDRTIDSHI KRLRKKFKAV DDSFEMIETL YGVXYRFREA

Validated NT Sequence

tttgtttnnc tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaagga agcttcggca acgcagacga ttgcgctggt tgatgatgac cgcaacatcc tgacctccgt ttccattgcg ctggagtcgg aaggttatcg cgtcgaaacc tataccgacg gcgcttctgc gctggatggg ttgatggcgc gtccgcccaa tcttgcgatc ttcgatatca agatgccgcg catggacggt atggagcttc tgcgccgcct gcgccagaag tccgatctgc cggtcatctt cctcacttcc aaggacgatg agatcgatga actcttcggc ctcaagatgg gggcggacga tttcatcacc aagccttttt cgcagcgtct tctggtcgag cgtgtcaagg ctgttctgcg ccgcgtggcg gcgcgcgacg gcacggcaaa gcccgccggc cagcaggcca agtcccttga gcgcggacag ctcgtcatgg atcaggagcg ccatacctgc acctggaagg gtgagccggt tacgctgact gttaccgaat ttctcatcct tcactcactt gcccagcgcc ccggcgtggt caaaagccgt gacgccctga tggatgcggc ctatgatgaa caggtctatg tggatgatcg caccatcgac agccacatca agcgtctgcg caagaagttc aaggcggttg acgacagctt cgagatgatc gaaacgctct acggcgtcng ttatcgtttc cgggaagcgt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctganttg gctgctgccn nnnnnnnnna aaaannnnaa aaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggaagct tcggcaacgc agacgattgc gctggttgat gatgaccgca acatcctgac ctccgtttcc attgcgctgg agtcggaagg ttatcgcgtc gaaacctata ccgacggcgc ttctgcgctg gatgggttga tggcgcgtcc gcccaatctt gcgatcttcg atatcaagat gccgcgcatg gacggtatgg agcttctgcg ccgcctgcgc cagaagtccg atctgccggt catcttcctc acttccaagg acgatgagat cgatgaactc ttcggcctca agatgggggc ggacgatttc atcaccaagc ctttttcgca gcgtcttctg gtcgagcgtg tcaaggctgt tctgcgccgc gtggcggcgc gcgacggcac ggcaaagccc gccggccagc aggccaagtc ccttgagcgc ggacagctcg tcatggatca ggagcgccat acctgcacct ggaagggtga gccggttacg ctgactgtta ccgaatttct catccttcac tcacttgccc agcgccccgg cgtggtcaaa agccgtgacg ccctgatgga tgcggcctat gatgaacagg tctatgtgga tgatcgcacc atcgacagcc acatcaagcg tctgcgcaag aagttcaagg cggttgacga cagcttcgag atgatcgaaa cgctctacgg cgtcggttat cgtttccggg aagcgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa