BrmeA.00545.a
Threonine synthase (EC 4.2.3.1)
| CENTER ID: | BrmeA.00545.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.00545.a.A1.GE33698 | full length | 1 | 463 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.00545.a.A1.PS01322 | full length | 1 | 463 |
Structures
4F4F
DEPOSITED: 5/10/2012
DETERMINATION: XRay
CLONE: BrmeA.00545.a.A1.GE33698
PROTEIN: BrmeA.00545.a.A1.PS01322
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128662 |
| PATRIC ID: | fig|224914.11.peg.1616 |
| RefSeq: | NP_540367.1 |
| UniProt: | Q8YFS0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYVSTRGEA PVLGFSDALL AGLARDGGLY LPQEYPQFTA EQIRALRGKS YVEVALAVLT PFTGGEIPAA DFERMVREAY GTFRHDAVCP LVQTDANEFV LELFHGPTLA FKDVAMQLLA RMMDYVLAQR GERATIVGAT SGDTGGAAIE AFGGRDNTDI FILFPNGRVS PVQQRQMTSS GFSNVHALSI EGNFDDCQNL VKGMFNDLEF CDALSLSGVN SINWARIMPQ VVYYFTAALS LGAPDRAVSF TVPTGNFGDI FAGYVAKRMG LPIEQLIIAT NDNDILSRTL ESGAYEMRGV AQTTSPSMDI QISSNFERLL FEAHGRDAAA VRGLMQGLKQ SGGFTISEKP LSAIRSEFSA GRSTVDETAA TIESVLSKDG YLLDPHSAIG VKVAREKASG TAPMVVLATA HPAKFPDAVK AACGVEPQLP AWLCDLMQRK ESFTVLHNEL KIVEEYVRHH SRA
NT Sequence
atgaaatatg taagtacgcg tggggaagca ccggttctgg gattcagcga tgcattgctc gcaggcctcg cccgtgatgg cggtctttat ctgccgcagg aatatccgca attcacagcc gaacagatcc gcgccctgcg tggaaaatcc tatgtcgaag tcgcgctggc cgtcctcacg ccctttaccg gcggtgaaat tccggcagcc gatttcgagc gcatggtccg cgaagcttac ggaaccttcc gtcacgatgc cgtctgcccg ttggtgcaga ccgacgcgaa cgaattcgtg ctggagcttt tccatggccc cacccttgcc ttcaaggatg tcgccatgca gcttctggcc cgcatgatgg attatgttct ggcacagcgc ggcgagcgcg caacgatcgt cggcgctaca tccggcgata cgggcggcgc agccatcgaa gcctttggcg ggcgcgacaa cacagacatt ttcatcctgt tccccaatgg gcgggtctcg ccggtacagc aacgccagat gacgtcttcc ggcttttcca atgtccatgc gctttccatc gagggcaatt tcgacgattg ccagaacctc gtaaaaggca tgttcaatga tctggaattc tgtgacgccc tgtcgctttc cggcgtgaac tcgatcaact gggcgcgcat catgccgcag gttgtctatt atttcacggc agcactcagc ctcggcgcac cggaccgcgc cgtgtccttc acggtgccca ccggcaattt cggcgatatt ttcgcaggct acgtcgccaa gcgcatgggc ctgcccatcg agcaactcat catcgccacc aacgacaacg atattctttc gcgcacgctg gaaagcgggg cctatgagat gcgcggcgta gcgcagacca cttcgccctc aatggatatc cagatttcct ccaatttcga gcggcttctg ttcgaggcac acgggcgcga tgcggcggcc gtgcgcgggc tgatgcaggg cctgaagcaa tctggcggat ttacgatttc cgaaaagccg ctttcggcca tccgcagcga attttcggct ggccgctcca ccgtcgatga gacggcggcg actatcgaat cggttctttc caaagacggc tatctgcttg atccgcattc ggcgatcggt gtgaaggtcg cgcgtgaaaa agcatccggc accgccccga tggtggttct ggcgaccgcc catccggcca aattcccgga tgcggtgaag gctgcatgtg gggtcgagcc gcaattgccg gcatggcttt gcgacctgat gcaacgaaaa gagagcttca cagttcttca caacgagctg aaaatcgtgg aagaatatgt gcgccaccat tcccgagcct ga
Details for BrmeA.00545.a.A1.GE33698
| HARVESTED ON: | 9/28/2011 |
| SEQUENCED ON: | 10/4/2011 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MKYVSTRGEA PVLGFSDALL AGLARDGGLY LPQEYPQFTA EQIRALRGKS YVEVALAVLT PFTGGEIPAA DFERMVREAY GTFRHDAVCP LVQTDANEFV LELFHGPTLA FKDVAMQLLA RMMDYVLAQR GERATIVGAT SGDTGGAAIE AFGGRDNTDI FILFPNGRVS PVQQRQMTSS GFSNVHALSI EGNFDDCQNL VKGMFNDLEF CDALSLSGVN SINWARIMPX VVYYFTAALS LGAPDRAVSF TVPTGNFGDI FAGYVAKRMG LPIEQLIIAT NDNDILSRTL ESGAYEMRGV AQTTSPSMDI QISSNFERLL FEAHGRDAAA VRGLMQGLKQ SGGFTISEKP LSAIRSEFSA GRSTVDETAA TIESVLSKDG YLLDPHSAIG VKVAREKASG TAPMVVLATA HPAKFPDAVK AACGVEPQLP AWLCDLMQRK ESFTVLHNEL KIVEEYVRHH SRA
Validated NT Sequence
attaggctcg ggaatggtgg cgcacatatt cttccacgat tttcagctcg ttgtgaagaa ctgtgaagct ctcttttcgt tgcatcaggt cgcaaagcca tgccggcaat tgcggctcga ccccacatgc agccttcacc gcatccggga atttggccgg atgggcggtc gccagaacca ccatcggggc ggtgccggat gctttttcac gcgcgacctt cacaccgatc gccgaatgcg gatcaagcag atagccgtct ttggaaagaa ccgattcgat agtcgccgcc gtctcatcga cggtggagcg gccagccgaa aattcgctgc ggatggccga aagcggcttt tcggaaatcg taaatccgcc agattgcttc aggccctgca tcagcccgcg cacggccgcc gcatcgcgcc cgtgtgcctc gaacagaagc cgctcgaaat tggaggaaat ctggatatcc attgagggcg aagtggtctg cgctacgccg cgcatctcat aggccccgct ttccagcgtg cgcgaaagaa tatcgttgtc gttggtggcg atgatgagtt gctcgatggg caggcccatg cgcttggcga cgtancctgc gaaaatatcg ccgaaattgc cggtgggcac cgtgaaggac acggcgcggt ccggtgcgcc gaggctgagt gctgccgtga aataatagac aaccngcggc atgatgcgcg cccagttgat cgagttcacg ccggaaagcg acagggcgtc acagaattcc agatcattga acatgccttt tacgaggttc tggcaatcgt cgaaattgcc ctcgatggaa agcgcatgga cattggaaaa gccggaagac gtcatctggc gttgctgtac cggcgagacc cgcccattgg ggaacaggat gaaaatgtct gtgttgtcgc gcccgccaaa ggcttcgatg gctgcgccgc ccgtatcgcc ggatgtagcg ccgacgatcg ttgcgcgctc gccgcgctgt gccagaacat aatccatcat gcgggccaga agctgcatgg cgacatcctt gaaggcaagg gtggggccat ggaaaagctc cagcacgaat tcgttcgcgt cggtctgcac caacgggcag acggcatcgt gacggaaggt tccgtaagct tcgcggacca tgcgctcgaa atcggctgcc ggaatttcac cgccggtaaa gggcgtgagg acggccagcg cgacttcgac ataggatttt ccacgcaggg cgcggatctg ttcggctgtg aattgcggat attcctgcgg cagataaaga ccgccatcac gggcgaggcc tgcgagcaat gcatcgctga atcccagaac cggtgcttcc ccacgcgtac ttacatattt catcgaacca ggaccctggg tctgagcttc cagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKYVSTRGE APVLGFSDAL LAGLARDGGL YLPQEYPQFT AEQIRALRGK SYVEVALAVL TPFTGGEIPA ADFERMVREA YGTFRHDAVC PLVQTDANEF VLELFHGPTL AFKDVAMQLL ARMMDYVLAQ RGERATIVGA TSGDTGGAAI EAFGGRDNTD IFILFPNGRV SPVQQRQMTS SGFSNVHALS IEGNFDDCQN LVKGMFNDLE FCDALSLSGV NSINWARIMP QVVYYFTAAL SLGAPDRAVS FTVPTGNFGD IFAGYVAKRM GLPIEQLIIA TNDNDILSRT LESGAYEMRG VAQTTSPSMD IQISSNFERL LFEAHGRDAA AVRGLMQGLK QSGGFTISEK PLSAIRSEFS AGRSTVDETA ATIESVLSKD GYLLDPHSAI GVKVAREKAS GTAPMVVLAT AHPAKFPDAV KAACGVEPQL PAWLCDLMQR KESFTVLHNE LKIVEEYVRH HSRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatatgta agtacgcgtg gggaagcacc ggttctggga ttcagcgatg cattgctcgc aggcctcgcc cgtgatggcg gtctttatct gccgcaggaa tatccgcaat tcacagccga acagatccgc gccctgcgtg gaaaatccta tgtcgaagtc gcgctggccg tcctcacgcc ctttaccggc ggtgaaattc cggcagccga tttcgagcgc atggtccgcg aagcttacgg aaccttccgt cacgatgccg tctgcccgtt ggtgcagacc gacgcgaacg aattcgtgct ggagcttttc catggcccca cccttgcctt caaggatgtc gccatgcagc ttctggcccg catgatggat tatgttctgg cacagcgcgg cgagcgcgca acgatcgtcg gcgctacatc cggcgatacg ggcggcgcag ccatcgaagc ctttggcggg cgcgacaaca cagacatttt catcctgttc cccaatgggc gggtctcgcc ggtacagcaa cgccagatga cgtcttccgg cttttccaat gtccatgcgc tttccatcga gggcaatttc gacgattgcc agaacctcgt aaaaggcatg ttcaatgatc tggaattctg tgacgccctg tcgctttccg gcgtgaactc gatcaactgg gcgcgcatca tgccgcaggt tgtctattat ttcacggcag cactcagcct cggcgcaccg gaccgcgccg tgtccttcac ggtgcccacc ggcaatttcg gcgatatttt cgcaggctac gtcgccaagc gcatgggcct gcccatcgag caactcatca tcgccaccaa cgacaacgat attctttcgc gcacgctgga aagcggggcc tatgagatgc gcggcgtagc gcagaccact tcgccctcaa tggatatcca gatttcctcc aatttcgagc ggcttctgtt cgaggcacac gggcgcgatg cggcggccgt gcgcgggctg atgcagggcc tgaagcaatc tggcggattt acgatttccg aaaagccgct ttcggccatc cgcagcgaat tttcggctgg ccgctccacc gtcgatgaga cggcggcgac tatcgaatcg gttctttcca aagacggcta tctgcttgat ccgcattcgg cgatcggtgt gaaggtcgcg cgtgaaaaag catccggcac cgccccgatg gtggttctgg cgaccgccca tccggccaaa ttcccggatg cggtgaaggc tgcatgtggg gtcgagccgc aattgccggc atggctttgc gacctgatgc aacgaaaaga gagcttcaca gttcttcaca acgagctgaa aatcgtggaa gaatatgtgc gccaccattc ccgagcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrmeA.00545.a.A1.PS01322
| PURIFICATION DATe: | 11/16/2011 |
| CONCENTRATION: | 53.43mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MKYVSTRGEA PVLGFSDALL AGLARDGGLY LPQEYPQFTA EQIRALRGKS YVEVALAVLT PFTGGEIPAA DFERMVREAY GTFRHDAVCP LVQTDANEFV LELFHGPTLA FKDVAMQLLA RMMDYVLAQR GERATIVGAT SGDTGGAAIE AFGGRDNTDI FILFPNGRVS PVQQRQMTSS GFSNVHALSI EGNFDDCQNL VKGMFNDLEF CDALSLSGVN SINWARIMPX VVYYFTAALS LGAPDRAVSF TVPTGNFGDI FAGYVAKRMG LPIEQLIIAT NDNDILSRTL ESGAYEMRGV AQTTSPSMDI QISSNFERLL FEAHGRDAAA VRGLMQGLKQ SGGFTISEKP LSAIRSEFSA GRSTVDETAA TIESVLSKDG YLLDPHSAIG VKVAREKASG TAPMVVLATA HPAKFPDAVK AACGVEPQLP AWLCDLMQRK ESFTVLHNEL KIVEEYVRHH SRA
Validated NT Sequence
attaggctcg ggaatggtgg cgcacatatt cttccacgat tttcagctcg ttgtgaagaa ctgtgaagct ctcttttcgt tgcatcaggt cgcaaagcca tgccggcaat tgcggctcga ccccacatgc agccttcacc gcatccggga atttggccgg atgggcggtc gccagaacca ccatcggggc ggtgccggat gctttttcac gcgcgacctt cacaccgatc gccgaatgcg gatcaagcag atagccgtct ttggaaagaa ccgattcgat agtcgccgcc gtctcatcga cggtggagcg gccagccgaa aattcgctgc ggatggccga aagcggcttt tcggaaatcg taaatccgcc agattgcttc aggccctgca tcagcccgcg cacggccgcc gcatcgcgcc cgtgtgcctc gaacagaagc cgctcgaaat tggaggaaat ctggatatcc attgagggcg aagtggtctg cgctacgccg cgcatctcat aggccccgct ttccagcgtg cgcgaaagaa tatcgttgtc gttggtggcg atgatgagtt gctcgatggg caggcccatg cgcttggcga cgtancctgc gaaaatatcg ccgaaattgc cggtgggcac cgtgaaggac acggcgcggt ccggtgcgcc gaggctgagt gctgccgtga aataatagac aaccngcggc atgatgcgcg cccagttgat cgagttcacg ccggaaagcg acagggcgtc acagaattcc agatcattga acatgccttt tacgaggttc tggcaatcgt cgaaattgcc ctcgatggaa agcgcatgga cattggaaaa gccggaagac gtcatctggc gttgctgtac cggcgagacc cgcccattgg ggaacaggat gaaaatgtct gtgttgtcgc gcccgccaaa ggcttcgatg gctgcgccgc ccgtatcgcc ggatgtagcg ccgacgatcg ttgcgcgctc gccgcgctgt gccagaacat aatccatcat gcgggccaga agctgcatgg cgacatcctt gaaggcaagg gtggggccat ggaaaagctc cagcacgaat tcgttcgcgt cggtctgcac caacgggcag acggcatcgt gacggaaggt tccgtaagct tcgcggacca tgcgctcgaa atcggctgcc ggaatttcac cgccggtaaa gggcgtgagg acggccagcg cgacttcgac ataggatttt ccacgcaggg cgcggatctg ttcggctgtg aattgcggat attcctgcgg cagataaaga ccgccatcac gggcgaggcc tgcgagcaat gcatcgctga atcccagaac cggtgcttcc ccacgcgtac ttacatattt catcgaacca ggaccctggg tctgagcttc cagggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKYVSTRGE APVLGFSDAL LAGLARDGGL YLPQEYPQFT AEQIRALRGK SYVEVALAVL TPFTGGEIPA ADFERMVREA YGTFRHDAVC PLVQTDANEF VLELFHGPTL AFKDVAMQLL ARMMDYVLAQ RGERATIVGA TSGDTGGAAI EAFGGRDNTD IFILFPNGRV SPVQQRQMTS SGFSNVHALS IEGNFDDCQN LVKGMFNDLE FCDALSLSGV NSINWARIMP QVVYYFTAAL SLGAPDRAVS FTVPTGNFGD IFAGYVAKRM GLPIEQLIIA TNDNDILSRT LESGAYEMRG VAQTTSPSMD IQISSNFERL LFEAHGRDAA AVRGLMQGLK QSGGFTISEK PLSAIRSEFS AGRSTVDETA ATIESVLSKD GYLLDPHSAI GVKVAREKAS GTAPMVVLAT AHPAKFPDAV KAACGVEPQL PAWLCDLMQR KESFTVLHNE LKIVEEYVRH HSRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATATGTA AGTACGCGTG GGGAAGCACC GGTTCTGGGA TTCAGCGATG CATTGCTCGC AGGCCTCGCC CGTGATGGCG GTCTTTATCT GCCGCAGGAA TATCCGCAAT TCACAGCCGA ACAGATCCGC GCCCTGCGTG GAAAATCCTA TGTCGAAGTC GCGCTGGCCG TCCTCACGCC CTTTACCGGC GGTGAAATTC CGGCAGCCGA TTTCGAGCGC ATGGTCCGCG AAGCTTACGG AACCTTCCGT CACGATGCCG TCTGCCCGTT GGTGCAGACC GACGCGAACG AATTCGTGCT GGAGCTTTTC CATGGCCCCA CCCTTGCCTT CAAGGATGTC GCCATGCAGC TTCTGGCCCG CATGATGGAT TATGTTCTGG CACAGCGCGG CGAGCGCGCA ACGATCGTCG GCGCTACATC CGGCGATACG GGCGGCGCAG CCATCGAAGC CTTTGGCGGG CGCGACAACA CAGACATTTT CATCCTGTTC CCCAATGGGC GGGTCTCGCC GGTACAGCAA CGCCAGATGA CGTCTTCCGG CTTTTCCAAT GTCCATGCGC TTTCCATCGA GGGCAATTTC GACGATTGCC AGAACCTCGT AAAAGGCATG TTCAATGATC TGGAATTCTG TGACGCCCTG TCGCTTTCCG GCGTGAACTC GATCAACTGG GCGCGCATCA TGCCGCAGGT TGTCTATTAT TTCACGGCAG CACTCAGCCT CGGCGCACCG GACCGCGCCG TGTCCTTCAC GGTGCCCACC GGCAATTTCG GCGATATTTT CGCAGGCTAC GTCGCCAAGC GCATGGGCCT GCCCATCGAG CAACTCATCA TCGCCACCAA CGACAACGAT ATTCTTTCGC GCACGCTGGA AAGCGGGGCC TATGAGATGC GCGGCGTAGC GCAGACCACT TCGCCCTCAA TGGATATCCA GATTTCCTCC AATTTCGAGC GGCTTCTGTT CGAGGCACAC GGGCGCGATG CGGCGGCCGT GCGCGGGCTG ATGCAGGGCC TGAAGCAATC TGGCGGATTT ACGATTTCCG AAAAGCCGCT TTCGGCCATC CGCAGCGAAT TTTCGGCTGG CCGCTCCACC GTCGATGAGA CGGCGGCGAC TATCGAATCG GTTCTTTCCA AAGACGGCTA TCTGCTTGAT CCGCATTCGG CGATCGGTGT GAAGGTCGCG CGTGAAAAAG CATCCGGCAC CGCCCCGATG GTGGTTCTGG CGACCGCCCA TCCGGCCAAA TTCCCGGATG CGGTGAAGGC TGCATGTGGG GTCGAGCCGC AATTGCCGGC ATGGCTTTGC GACCTGATGC AACGAAAAGA GAGCTTCACA GTTCTTCACA ACGAGCTGAA AATCGTGGAA GAATATGTGC GCCACCATTC CCGAGCCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA