SSGCID: Target Page

BrmeA.00507.a

Ketol-acid reductoisomerase (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (Alpha-keto-beta-hydroxylacil reductoisomerase)

CENTER ID:	BrmeA.00507.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00507.a.A1.GE33696	full length		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00507.a.A1.PS01290	full length		1	339

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.700
RefSeq:	NP_539541.1
UniProt:	Q8YI21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVYYDRDAD VNLIKSKKVV IVGYGSQGRA HALNLKDSGA ANVRVALREG SATVQKAQAD GFEVMNVADA AKWADLMMMA TPDELQADIY RDHIHNNLRD GAAIAFAHGL NVHCGLIEPK KTVDVMMIAP KGPGHTVRGE YQKGGGVPCL IAIHQDASGN AHDLALSYAS GVGGGRSGVI ETTFKEECET DLFGEQAVLC GGVVELIRTG FEVLVEAGYA PEMAYFECLN EMKLIVDLIY EGGIANMNYS ISNTAEWGEY VTGPRIITAE TKAEMKRVLK DIQTGKFTSD WMQEWKAGAA RFKGIRRLND AHQIEEVGGK LRAMMPWIEK NKLVDKARN

NT Sequence

atgcgcgttt attacgatcg ggatgcagac gttaacctga tcaagtcgaa gaaggtggtt atcgtcggtt atggcagcca gggccgcgcc catgcgctca acctgaagga ctctggcgcg gccaatgtgc gcgttgccct tcgtgaaggc tccgccacgg ttcagaaggc acaggccgat ggctttgaag tcatgaacgt cgccgatgcc gccaagtggg ccgacctcat gatgatggcg acccctgacg aattgcaggc cgacatctac cgcgaccaca tccacaacaa tctgcgcgat ggcgctgcga ttgcttttgc ccatggcctc aacgtgcatt gcggcctgat tgagccgaag aagacggtcg atgtcatgat gatcgcgccg aagggtcccg gccacacggt tcgcggtgaa tatcagaagg gcggcggcgt tccctgcctc atcgccattc atcaggatgc atctggcaat gcccatgatc tggccctttc ctacgcttcg ggcgttggcg gcggccgttc gggcgttatc gagacgacct tcaaggaaga gtgcgaaacc gatctcttcg gcgaacaggc tgttctgtgc ggcggcgtgg tcgagctgat ccgcaccggc tttgaagttc tggtcgaagc tggctatgcg ccggaaatgg cctatttcga gtgcctgaac gaaatgaagc tgatcgtgga cctgatctac gaaggcggca ttgccaacat gaactattcg atctccaaca ctgccgagtg gggcgaatat gtgaccggcc cgcgcatcat caccgccgaa accaaggccg aaatgaagcg tgttctgaag gatatccaga ccggcaagtt cacctctgac tggatgcagg aatggaaagc cggcgctgcc cgcttcaagg gtatccgccg tctcaacgac gcccatcaga tcgaagaagt cggtggcaag ctacgcgcca tgatgccgtg gatcgaaaag aacaaactgg tcgataaagc ccgcaactga

Details for BrmeA.00507.a.A1.GE33696

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	86

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRVYYDRDA DVNLIKSKKV VIVGYGSQGR AHALNLKDSG AANVRVALRE GSATVQKAQA DGFEVMNVAD AAKWADLMMM ATPDELQADI YRDHIHNNLR DGAAIAFAHG LNVHCGLIEP KKTVDVMMIA PKGPGHTVRG EYQKGGGVPC LIAIHQDASG NAHDLALSYA SGVGGGRSGV IETTFKEECE TDLFGEQAVL CGGVVELIRT GFEVLVEAGY APEMAYFECL NEMKLIVDLI YEGGIANMNY SISNTAEWGE YVTGPRIITA ETXGRNEACS EGYPDRQVHL

Validated NT Sequence

attttnttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg tttattacga tcgggatgca gacgttaacc tgatcaagtc gaagaaggtg gttatcgtcg gttatggcag ccagggccgc gcccatgcgc tcaacctgaa ggactctggc gcggccaatg tgcgcgttgc ccttcgtgaa ggctccgcca cggttcagaa ggcacaggcc gatggctttg aagtcatgaa cgtcgccgat gccgccaagt gggccgacct catgatgatg gcgacccctg acgaattgca ggccgacatc taccgcgacc acatccacaa caatctgcgc gatggcgctg cgattgcttt tgcccatggc ctcaacgtgc attgcggcct gattgagccg aagaagacgg tcgatgtcat gatgatcgcg ccgaagggtc ccggccacac ggttcgcggt gaatatcaga agggcggcgg cgttccctgc ctcatcgcca ttcatcagga tgcatctggc aatgcccatg atctggccct ttcctacgct tcgggcgttg gcggcggccg ttcgggcgtt atcgagacga ccttcaagga agagtgcgaa accgatctct tcggcgaaca ggctgttctg tgcggcggcg tggtcgagct gatccgcacc ggctttgaag ttctggtcga agctggctat gcgccggaaa tggcctattt cgagtgcctg aacgaaatga agctgatcgt ggacctgatc tacgaaggcg gcattgccaa catgaactat tcgatctcca acactgccga gtggggcgaa tatgtgaccg gcccgcgcat catcaccgcc gaaaccnang gccgaaatga agcgtgttct gaaggatatc cagaccggca agttcacctc tgactggatg cnggaatggn aagccggcgc tgcccgcttc angggnatcc gccgtctcaa cgacncccat canatcnana ntnggtggna nctacncncn tgntncnngg ntcnaaaaga acnaactnnn ganannncng nannannnnn nnacnannna nnnngcannt nnnnnnngnn nnnnnncnnn nannnnnnnn ntnnnnnnnn nnnnnnnann nnannnngnn nnnnnnnnnn tnnntnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgtttat tacgatcggg atgcagacgt taacctgatc aagtcgaaga aggtggttat cgtcggttat ggcagccagg gccgcgccca tgcgctcaac ctgaaggact ctggcgcggc caatgtgcgc gttgcccttc gtgaaggctc cgccacggtt cagaaggcac aggccgatgg ctttgaagtc atgaacgtcg ccgatgccgc caagtgggcc gacctcatga tgatggcgac ccctgacgaa ttgcaggccg acatctaccg cgaccacatc cacaacaatc tgcgcgatgg cgctgcgatt gcttttgccc atggcctcaa cgtgcattgc ggcctgattg agccgaagaa gacggtcgat gtcatgatga tcgcgccgaa gggtcccggc cacacggttc gcggtgaata tcagaagggc ggcggcgttc cctgcctcat cgccattcat caggatgcat ctggcaatgc ccatgatctg gccctttcct acgcttcggg cgttggcggc ggccgttcgg gcgttatcga gacgaccttc aaggaagagt gcgaaaccga tctcttcggc gaacaggctg ttctgtgcgg cggcgtggtc gagctgatcc gcaccggctt tgaagttctg gtcgaagctg gctatgcgcc ggaaatggcc tatttcgagt gcctgaacga aatgaagctg atcgtggacc tgatctacga aggcggcatt gccaacatga actattcgat ctccaacact gccgagtggg gcgaatatgt gaccggcccg cgcatcatca ccgccgaaac caaggccgaa atgaagcgtg ttctgaagga tatccagacc ggcaagttca cctctgactg gatgcaggaa tggaaagccg gcgctgcccg cttcaagggt atccgccgtc tcaacgacgc ccatcagatc gaagaagtcg gtggcaagct acgcgccatg atgccgtgga tcgaaaagaa caaactggtc gataaagccc gcaactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BrmeA.00507.a.A1.PS01290

PURIFICATION DATe:	11/16/2011
CONCENTRATION:	7.46mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	86

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCGTTTAT TACGATCGGG ATGCAGACGT TAACCTGATC AAGTCGAAGA AGGTGGTTAT CGTCGGTTAT GGCAGCCAGG GCCGCGCCCA TGCGCTCAAC CTGAAGGACT CTGGCGCGGC CAATGTGCGC GTTGCCCTTC GTGAAGGCTC CGCCACGGTT CAGAAGGCAC AGGCCGATGG CTTTGAAGTC ATGAACGTCG CCGATGCCGC CAAGTGGGCC GACCTCATGA TGATGGCGAC CCCTGACGAA TTGCAGGCCG ACATCTACCG CGACCACATC CACAACAATC TGCGCGATGG CGCTGCGATT GCTTTTGCCC ATGGCCTCAA CGTGCATTGC GGCCTGATTG AGCCGAAGAA GACGGTCGAT GTCATGATGA TCGCGCCGAA GGGTCCCGGC CACACGGTTC GCGGTGAATA TCAGAAGGGC GGCGGCGTTC CCTGCCTCAT CGCCATTCAT CAGGATGCAT CTGGCAATGC CCATGATCTG GCCCTTTCCT ACGCTTCGGG CGTTGGCGGC GGCCGTTCGG GCGTTATCGA GACGACCTTC AAGGAAGAGT GCGAAACCGA TCTCTTCGGC GAACAGGCTG TTCTGTGCGG CGGCGTGGTC GAGCTGATCC GCACCGGCTT TGAAGTTCTG GTCGAAGCTG GCTATGCGCC GGAAATGGCC TATTTCGAGT GCCTGAACGA AATGAAGCTG ATCGTGGACC TGATCTACGA AGGCGGCATT GCCAACATGA ACTATTCGAT CTCCAACACT GCCGAGTGGG GCGAATATGT GACCGGCCCG CGCATCATCA CCGCCGAAAC CAAGGCCGAA ATGAAGCGTG TTCTGAAGGA TATCCAGACC GGCAAGTTCA CCTCTGACTG GATGCAGGAA TGGAAAGCCG GCGCTGCCCG CTTCAAGGGT ATCCGCCGTC TCAACGACGC CCATCAGATC GAAGAAGTCG GTGGCAAGCT ACGCGCCATG ATGCCGTGGA TCGAAAAGAA CAAACTGGTC GATAAAGCCC GCAACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA