BrmeA.00363.a

Zinc protease

CENTER ID: BrmeA.00363.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00363.a.A1.GE33713 full length 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00363.a.A1.PS01313 full length 1 464
BrmeA.00363.a.B2.PW39342 AlphaFold domain 5 437

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.3393
RefSeq: NP_542015.1
UniProt: Q8YB63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQAALPEIS RLDGVSNFTL PNGMQVVVIP DHRAPVVTQM VWYHVGAADE APGVSGIAHF LEHLMFKGTK NHPAGEFSAR IASIGGQENA FTSYDYTAYF QRVSPEALEM VMDFESDRME NLVLDEEAVK TEREVILEER RMRIDSNPGA MLMENTDAVL FYNHPYRKPV IGWQQEMEKL SLKNAIDFYN QYYTPNNATL VIAGDVTPER VRELAMKTWA NVHKRAEVLL RERPQEPAKH AARVVTLHDE RVSTPSFRIS WLVPSYANEK RFANVKPGDA PALDLLSEIL GGSQLSRLYQ QLIVKQGIAA ETGASYDGDA LDDGTFSVYG VPRNGASLGD VEKAVAAQVD RIIRDGVTQA ELDQARNRFL KAVIFARDSQ TGMARIYGSA LSVGQTVDDI QKWPDLIKSV TVDQIKDVAR RYLVKDQAVT SYLLPPDTEA ESARKKPNAP ANDASASGAG GAIQ
NT Sequence
gtggcgcagg cagccctgcc ggagatttcc cggttggacg gggtcagcaa tttcacgctt ccaaacggta tgcaggtggt ggtcattccc gatcaccgtg cccctgtcgt cacccagatg gtctggtatc atgtcggtgc ggcggatgaa gcccccggcg tttcgggcat tgcgcatttt ctcgaacatc tgatgttcaa gggcaccaaa aaccacccgg caggcgagtt ttcggccaga atcgcctcaa tcggcgggca ggaaaacgct ttcacctctt atgattatac ggcctacttc cagcgtgttt cgccggaagc gctggaaatg gtgatggatt ttgaatccga ccggatggaa aatctggttc tggacgagga agcggtgaag accgagcgcg aggtcattct ggaagagcgc cggatgcgca tcgattccaa ccctggcgca atgctcatgg aaaacactga tgctgtgctt ttctataacc atccctatcg caagccggtg atcggctggc agcaggaaat ggaaaagctg agcctgaaga atgccatcga tttctacaat caatattata cgcccaataa tgcgacgctg gtgattgcgg gtgatgtgac gccggagcgt gtgcgcgagc ttgcgatgaa gacctgggcg aacgtccaca aacgcgccga ggttttgctg cgcgaacgcc cgcaggaacc ggcgaaacat gcagcccgtg tcgtaaccct gcatgatgag cgtgtcagca caccctcctt ccgcatttca tggctggtgc cttcctatgc caatgaaaag cgatttgcga atgtgaaacc cggcgatgcg ccggcgcttg atcttttgag tgaaatactg ggcgggtcgc agctttcgcg gctttaccag caattgatcg tcaagcaggg catcgctgct gaaacaggcg caagctatga cggcgatgcg ctggatgacg gcacgttctc ggtctatggc gtaccgcgca acggcgcatc gcttggcgat gtcgaaaaag ctgtcgcagc gcaagtggat cgtatcatcc gggatggtgt cacacaggct gaactcgatc aggcgcgcaa ccgtttcctc aaggcggtga ttttcgcgcg cgacagccag accggcatgg cgcgcatcta tggttcggcg ctttcggttg gccagacggt ggatgatatc cagaaatggc ccgatctcat caaaagcgta accgtcgatc agatcaagga tgtagccagg cgttatctgg tcaaggacca agccgtgacg agctatcttc tgccgcccga taccgaagca gaaagcgccc gcaagaagcc gaacgcgcca gcaaatgacg caagcgcaag cggtgcggga ggagcgatcc agtga
Details for BrmeA.00363.a.A1.GE33713
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MVAQAALPEI SRLDGVSNFT LPNGMQVVVI PDHRAPVVTQ MVWYHVGAAD EAPGVSGIAH FLEHLMFKGT KNHPAGEFSA RIASIGGQEN AFTSYDYTAY FQRVSPEALE MVMDFESDRM ENLVLDEEAV KTEREVILEE RRMRIDSNPG AMLMENTDAV LFYNHPYRKP VIGWQQEMEK LSLKNAIDFY NQYYTPNNAT LVIAGDVTPE RVRELAMKTW ANVHKRAEVL LRERPQEPAK HAARVVTLHD ERVSTPSFRI SWLVPSYANE KRFANVKPGD APALDLLSEI LGGSQLSRLY QQLIVKQGIA AETGASYDGD ALDDGTFSVY GVPRNGASLG DVEKAVAAQV DRIIRDGVTQ AELDQARNRF LKAVIFARDS QTGMARIYGS ALSVGQTVDD IQKWPDLIKS VTVDQIKDVA RRYLVKDQAV TSYLLPPDTE AESARKKPNA PANDASASGA GGAI
Validated NT Sequence
ctggatcgct cctcccgcac cgcttgcgct tgcgtcattt gctggcgcgt tcggcttctt gcgggcgctt tctgcttcgg tatcgggcgg cagaagatag ctcgtcacgg cttggtcctt gaccagataa cgcctggcta catccttgat ctgatcgacg gttacgcttt tgatgagatc gggccatttc tggatatcat ccaccgtctg gccaaccgaa agcgccgaac catagatgcg cgccatgccg gtctggctgt cgcgcgcgaa aatcaccgcc ttgaggaaac ggttgcgcgc ctgatcgagt tcagcctgtg tgacaccatc ccggatgata cgatccactt gcgctgcgac agctttttcg acatcgccaa gcgatgcgcc gttgcgcggt acgccataga ccgagaacgt gccgtcatcc agcgcatcgc cgtcatagct tgcgcctgtt tcagcagcga tgccctgctt gacgatcaat tgctggtaaa gccgcgaaag ctgcgacccg cccagtattt cactcaaaag atcaagcgcc ggcgcatcgc cgggtttcac attcgcaaat cgcttttcat tggcatagga aggcaccagc catgaaatgc ggaaggaggg tgtgctgaca cgctcatcat gcagggttac gacacgggct gcatgtttcg ccggttcctg cgggcgttcg cgcagcaaaa cctcggcgcg tttgtggacg ttcgcccagg tcttcatcgc aagctcgcgc acacgctcng gcgtcacatc acccgcaatc accagcgtcg cattattggg cgtataatat tgattgtaga aatcgatggc attcttcagg ctcagctttt ccatttcctg ctgccagccg atcaccggct tgcgataggg atggttatag aaaagcacag catcagtgtt ttccatgagc attgcgccag ggttggaatc gatgcgcatc cggcgctctt ccagaatgac ctcgcgctcg gtcttcaccg cttcctcgtc cagaaccaga ttttccatcc ggtcggattc aaaatccatc accatttcca gcgcttccgg cgaaacacgc tggaagtagg ccgtataatc ataagaggtg aaagcgtttt cctgcccgcc gattgaggcg attctggccg aaaactcgcc tgccgggtgg tttttggtgc ccttgaacat cagatgttcg agaaaatgcg caatgcccga aacgccgggg gcttcatccg ccgcaccgac atgataccag accatctggg tgacgacagg ggcacggtga tcgggaatga ccaccacctg cataccgttt ggaagcgtga aattgctgac cccgtccaac cgggaaatct ccggcagggc tgcctgcgcc accatcgaac caggaccctg ggtctgagct tccagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQAALPE ISRLDGVSNF TLPNGMQVVV IPDHRAPVVT QMVWYHVGAA DEAPGVSGIA HFLEHLMFKG TKNHPAGEFS ARIASIGGQE NAFTSYDYTA YFQRVSPEAL EMVMDFESDR MENLVLDEEA VKTEREVILE ERRMRIDSNP GAMLMENTDA VLFYNHPYRK PVIGWQQEME KLSLKNAIDF YNQYYTPNNA TLVIAGDVTP ERVRELAMKT WANVHKRAEV LLRERPQEPA KHAARVVTLH DERVSTPSFR ISWLVPSYAN EKRFANVKPG DAPALDLLSE ILGGSQLSRL YQQLIVKQGI AAETGASYDG DALDDGTFSV YGVPRNGASL GDVEKAVAAQ VDRIIRDGVT QAELDQARNR FLKAVIFARD SQTGMARIYG SALSVGQTVD DIQKWPDLIK SVTVDQIKDV ARRYLVKDQA VTSYLLPPDT EAESARKKPN APANDASASG AGGAIQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgcag gcagccctgc cggagatttc ccggttggac ggggtcagca atttcacgct tccaaacggt atgcaggtgg tggtcattcc cgatcaccgt gcccctgtcg tcacccagat ggtctggtat catgtcggtg cggcggatga agcccccggc gtttcgggca ttgcgcattt tctcgaacat ctgatgttca agggcaccaa aaaccacccg gcaggcgagt tttcggccag aatcgcctca atcggcgggc aggaaaacgc tttcacctct tatgattata cggcctactt ccagcgtgtt tcgccggaag cgctggaaat ggtgatggat tttgaatccg accggatgga aaatctggtt ctggacgagg aagcggtgaa gaccgagcgc gaggtcattc tggaagagcg ccggatgcgc atcgattcca accctggcgc aatgctcatg gaaaacactg atgctgtgct tttctataac catccctatc gcaagccggt gatcggctgg cagcaggaaa tggaaaagct gagcctgaag aatgccatcg atttctacaa tcaatattat acgcccaata atgcgacgct ggtgattgcg ggtgatgtga cgccggagcg tgtgcgcgag cttgcgatga agacctgggc gaacgtccac aaacgcgccg aggttttgct gcgcgaacgc ccgcaggaac cggcgaaaca tgcagcccgt gtcgtaaccc tgcatgatga gcgtgtcagc acaccctcct tccgcatttc atggctggtg ccttcctatg ccaatgaaaa gcgatttgcg aatgtgaaac ccggcgatgc gccggcgctt gatcttttga gtgaaatact gggcgggtcg cagctttcgc ggctttacca gcaattgatc gtcaagcagg gcatcgctgc tgaaacaggc gcaagctatg acggcgatgc gctggatgac ggcacgttct cggtctatgg cgtaccgcgc aacggcgcat cgcttggcga tgtcgaaaaa gctgtcgcag cgcaagtgga tcgtatcatc cgggatggtg tcacacaggc tgaactcgat caggcgcgca accgtttcct caaggcggtg attttcgcgc gcgacagcca gaccggcatg gcgcgcatct atggttcggc gctttcggtt ggccagacgg tggatgatat ccagaaatgg cccgatctca tcaaaagcgt aaccgtcgat cagatcaagg atgtagccag gcgttatctg gtcaaggacc aagccgtgac gagctatctt ctgccgcccg ataccgaagc agaaagcgcc cgcaagaagc cgaacgcgcc agcaaatgac gcaagcgcaa gcggtgcggg aggagcgatc cagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrmeA.00363.a.A1.PS01313
PURIFICATION DATe: 12/22/2011
CONCENTRATION: 26.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MVAQAALPEI SRLDGVSNFT LPNGMQVVVI PDHRAPVVTQ MVWYHVGAAD EAPGVSGIAH FLEHLMFKGT KNHPAGEFSA RIASIGGQEN AFTSYDYTAY FQRVSPEALE MVMDFESDRM ENLVLDEEAV KTEREVILEE RRMRIDSNPG AMLMENTDAV LFYNHPYRKP VIGWQQEMEK LSLKNAIDFY NQYYTPNNAT LVIAGDVTPE RVRELAMKTW ANVHKRAEVL LRERPQEPAK HAARVVTLHD ERVSTPSFRI SWLVPSYANE KRFANVKPGD APALDLLSEI LGGSQLSRLY QQLIVKQGIA AETGASYDGD ALDDGTFSVY GVPRNGASLG DVEKAVAAQV DRIIRDGVTQ AELDQARNRF LKAVIFARDS QTGMARIYGS ALSVGQTVDD IQKWPDLIKS VTVDQIKDVA RRYLVKDQAV TSYLLPPDTE AESARKKPNA PANDASASGA GGAI
Validated NT Sequence
ctggatcgct cctcccgcac cgcttgcgct tgcgtcattt gctggcgcgt tcggcttctt gcgggcgctt tctgcttcgg tatcgggcgg cagaagatag ctcgtcacgg cttggtcctt gaccagataa cgcctggcta catccttgat ctgatcgacg gttacgcttt tgatgagatc gggccatttc tggatatcat ccaccgtctg gccaaccgaa agcgccgaac catagatgcg cgccatgccg gtctggctgt cgcgcgcgaa aatcaccgcc ttgaggaaac ggttgcgcgc ctgatcgagt tcagcctgtg tgacaccatc ccggatgata cgatccactt gcgctgcgac agctttttcg acatcgccaa gcgatgcgcc gttgcgcggt acgccataga ccgagaacgt gccgtcatcc agcgcatcgc cgtcatagct tgcgcctgtt tcagcagcga tgccctgctt gacgatcaat tgctggtaaa gccgcgaaag ctgcgacccg cccagtattt cactcaaaag atcaagcgcc ggcgcatcgc cgggtttcac attcgcaaat cgcttttcat tggcatagga aggcaccagc catgaaatgc ggaaggaggg tgtgctgaca cgctcatcat gcagggttac gacacgggct gcatgtttcg ccggttcctg cgggcgttcg cgcagcaaaa cctcggcgcg tttgtggacg ttcgcccagg tcttcatcgc aagctcgcgc acacgctcng gcgtcacatc acccgcaatc accagcgtcg cattattggg cgtataatat tgattgtaga aatcgatggc attcttcagg ctcagctttt ccatttcctg ctgccagccg atcaccggct tgcgataggg atggttatag aaaagcacag catcagtgtt ttccatgagc attgcgccag ggttggaatc gatgcgcatc cggcgctctt ccagaatgac ctcgcgctcg gtcttcaccg cttcctcgtc cagaaccaga ttttccatcc ggtcggattc aaaatccatc accatttcca gcgcttccgg cgaaacacgc tggaagtagg ccgtataatc ataagaggtg aaagcgtttt cctgcccgcc gattgaggcg attctggccg aaaactcgcc tgccgggtgg tttttggtgc ccttgaacat cagatgttcg agaaaatgcg caatgcccga aacgccgggg gcttcatccg ccgcaccgac atgataccag accatctggg tgacgacagg ggcacggtga tcgggaatga ccaccacctg cataccgttt ggaagcgtga aattgctgac cccgtccaac cgggaaatct ccggcagggc tgcctgcgcc accatcgaac caggaccctg ggtctgagct tccagggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQAALPE ISRLDGVSNF TLPNGMQVVV IPDHRAPVVT QMVWYHVGAA DEAPGVSGIA HFLEHLMFKG TKNHPAGEFS ARIASIGGQE NAFTSYDYTA YFQRVSPEAL EMVMDFESDR MENLVLDEEA VKTEREVILE ERRMRIDSNP GAMLMENTDA VLFYNHPYRK PVIGWQQEME KLSLKNAIDF YNQYYTPNNA TLVIAGDVTP ERVRELAMKT WANVHKRAEV LLRERPQEPA KHAARVVTLH DERVSTPSFR ISWLVPSYAN EKRFANVKPG DAPALDLLSE ILGGSQLSRL YQQLIVKQGI AAETGASYDG DALDDGTFSV YGVPRNGASL GDVEKAVAAQ VDRIIRDGVT QAELDQARNR FLKAVIFARD SQTGMARIYG SALSVGQTVD DIQKWPDLIK SVTVDQIKDV ARRYLVKDQA VTSYLLPPDT EAESARKKPN APANDASASG AGGAIQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCGCAG GCAGCCCTGC CGGAGATTTC CCGGTTGGAC GGGGTCAGCA ATTTCACGCT TCCAAACGGT ATGCAGGTGG TGGTCATTCC CGATCACCGT GCCCCTGTCG TCACCCAGAT GGTCTGGTAT CATGTCGGTG CGGCGGATGA AGCCCCCGGC GTTTCGGGCA TTGCGCATTT TCTCGAACAT CTGATGTTCA AGGGCACCAA AAACCACCCG GCAGGCGAGT TTTCGGCCAG AATCGCCTCA ATCGGCGGGC AGGAAAACGC TTTCACCTCT TATGATTATA CGGCCTACTT CCAGCGTGTT TCGCCGGAAG CGCTGGAAAT GGTGATGGAT TTTGAATCCG ACCGGATGGA AAATCTGGTT CTGGACGAGG AAGCGGTGAA GACCGAGCGC GAGGTCATTC TGGAAGAGCG CCGGATGCGC ATCGATTCCA ACCCTGGCGC AATGCTCATG GAAAACACTG ATGCTGTGCT TTTCTATAAC CATCCCTATC GCAAGCCGGT GATCGGCTGG CAGCAGGAAA TGGAAAAGCT GAGCCTGAAG AATGCCATCG ATTTCTACAA TCAATATTAT ACGCCCAATA ATGCGACGCT GGTGATTGCG GGTGATGTGA CGCCGGAGCG TGTGCGCGAG CTTGCGATGA AGACCTGGGC GAACGTCCAC AAACGCGCCG AGGTTTTGCT GCGCGAACGC CCGCAGGAAC CGGCGAAACA TGCAGCCCGT GTCGTAACCC TGCATGATGA GCGTGTCAGC ACACCCTCCT TCCGCATTTC ATGGCTGGTG CCTTCCTATG CCAATGAAAA GCGATTTGCG AATGTGAAAC CCGGCGATGC GCCGGCGCTT GATCTTTTGA GTGAAATACT GGGCGGGTCG CAGCTTTCGC GGCTTTACCA GCAATTGATC GTCAAGCAGG GCATCGCTGC TGAAACAGGC GCAAGCTATG ACGGCGATGC GCTGGATGAC GGCACGTTCT CGGTCTATGG CGTACCGCGC AACGGCGCAT CGCTTGGCGA TGTCGAAAAA GCTGTCGCAG CGCAAGTGGA TCGTATCATC CGGGATGGTG TCACACAGGC TGAACTCGAT CAGGCGCGCA ACCGTTTCCT CAAGGCGGTG ATTTTCGCGC GCGACAGCCA GACCGGCATG GCGCGCATCT ATGGTTCGGC GCTTTCGGTT GGCCAGACGG TGGATGATAT CCAGAAATGG CCCGATCTCA TCAAAAGCGT AACCGTCGAT CAGATCAAGG ATGTAGCCAG GCGTTATCTG GTCAAGGACC AAGCCGTGAC GAGCTATCTT CTGCCGCCCG ATACCGAAGC AGAAAGCGCC CGCAAGAAGC CGAACGCGCC AGCAAATGAC GCAAGCGCAA GCGGTGCGGG AGGAGCGATC CAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrmeA.00363.a.B2.PW39342
PURIFICATION DATe: 12/2/2024
CONCENTRATION: 18.1mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 23
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHAL PEISRLDGVS NFTLPNGMQV VVIPDHRAPV VTQMVWYHVG AADEAPGVSG IAHFLEHLMF KGTKNHPAGE FSARIASIGG QENAFTSYDY TAYFQRVSPE ALEMVMDFES DRMENLVLDE EAVKTEREVI LEERRMRIDS NPGAMLMENT DAVLFYNHPY RKPVIGWQQE MEKLSLKNAI DFYNQYYTPN NATLVIAGDV TPERVRELAM KTWANVHKRA EVLLRERPQE PAKHAARVVT LHDERVSTPS FRISWLVPSY ANEKRFANVK PGDAPALDLL SEILGGSQLS RLYQQLIVKQ GIAAETGASY DGDALDDGTF SVYGVPRNGA SLGDVEKAVA AQVDRIIRDG VTQAELDQAR NRFLKAVIFA RDSQTGMARI YGSALSVGQT VDDIQKWPDL IKSVTVDQIK DVARRYLVKD QAVTSYLLPP D
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCCTGC CGGAGATTTC CCGGTTGGAC GGGGTCAGCA ATTTCACGCT TCCAAACGGT ATGCAGGTGG TGGTCATTCC CGATCACCGT GCCCCTGTCG TCACCCAGAT GGTCTGGTAT CATGTCGGTG CGGCGGATGA AGCCCCCGGC GTTTCGGGCA TTGCGCATTT TCTCGAACAT CTGATGTTCA AGGGCACCAA AAACCACCCG GCAGGCGAGT TTTCGGCCAG AATCGCCTCA ATCGGCGGGC AGGAAAACGC TTTCACCTCT TATGATTATA CGGCCTACTT CCAGCGTGTT TCGCCGGAAG CGCTGGAAAT GGTGATGGAT TTTGAATCCG ACCGGATGGA AAATCTGGTT CTGGACGAGG AAGCGGTGAA GACCGAGCGC GAGGTCATTC TGGAAGAGCG CCGGATGCGC ATCGATTCCA ACCCTGGCGC AATGCTCATG GAAAACACTG ATGCTGTGCT TTTCTATAAC CATCCCTATC GCAAGCCGGT GATCGGCTGG CAGCAGGAAA TGGAAAAGCT GAGCCTGAAG AATGCCATCG ATTTCTACAA TCAATATTAT ACGCCCAATA ATGCGACGCT GGTGATTGCG GGTGATGTGA CGCCGGAGCG TGTGCGCGAG CTTGCGATGA AGACCTGGGC GAACGTCCAC AAACGCGCCG AGGTTTTGCT GCGCGAACGC CCGCAGGAAC CGGCGAAACA TGCAGCCCGT GTCGTAACCC TGCATGATGA GCGTGTCAGC ACACCCTCCT TCCGCATTTC ATGGCTGGTG CCTTCCTATG CCAATGAAAA GCGATTTGCG AATGTGAAAC CCGGCGATGC GCCGGCGCTT GATCTTTTGA GTGAAATACT GGGCGGGTCG CAGCTTTCGC GGCTTTACCA GCAATTGATC GTCAAGCAGG GCATCGCTGC TGAAACAGGC GCAAGCTATG ACGGCGATGC GCTGGATGAC GGCACGTTCT CGGTCTATGG CGTACCGCGC AACGGCGCAT CGCTTGGCGA TGTCGAAAAA GCTGTCGCAG CGCAAGTGGA TCGTATCATC CGGGATGGTG TCACACAGGC TGAACTCGAT CAGGCGCGCA ACCGTTTCCT CAAGGCGGTG ATTTTCGCGC GCGACAGCCA GACCGGCATG GCGCGCATCT ATGGTTCGGC GCTTTCGGTT GGCCAGACGG TGGATGATAT CCAGAAATGG CCCGATCTCA TCAAAAGCGT AACCGTCGAT CAGATCAAGG ATGTAGCCAG GCGTTATCTG GTCAAGGACC AAGCCGTGAC GAGCTATCTT CTGCCGCCCG ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT