BrmeA.00204.a

Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (Carbamoyl-phosphate synthetase glutamine chain)

CENTER ID: BrmeA.00204.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00204.a.A1.GE33695 full length 1 407
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00204.a.A1.PS01394 full length 1 407
BrmeA.00204.a.A1.PS01393 full length 1 407

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.592
RefSeq: NP_539443.2
UniProt: Q8YIB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTETTPKTAP WTVQKRTAVL VLADGTVIEG KGLGATGAVE AEVVFNTALT GYEEILTDPS YAGQIVTFTF PHIGNVGANA EDIEDLTPAN RHGAVGAIFK ADITAPSNFR AAKDLDSWLK HRGIIALAGI DTRALTALIR ERGAQNAVIA HDPNGNFDLD ALKARAANWC GLENLDLAKD VTIGQSLVWK ELPWTLQDGY GEQDAPQYHV VALDFGVKRN ILRLLTGLGA KVTVLPATAT AEDVLAHNPD GVFLSNGPGD PAATGEYAVP TIGKLVETGI PLFGICLGHQ MLALALGGRT EKMHQGHHGA NHPVKDYTTG KVEIVSMNHG FAVDSDSLPE NVEETHVSLF DGTNCGLRVV GKPVFSVQHH PEASPGPQDS HYLFRRFINL IRERKGQAPL PEREQAA
NT Sequence
atgacagcac aggctggcga tgccaacgaa acaggagtgc acactatgac tgaaacgacc ccgaaaacag caccctggac cgttcagaag cgaaccgccg ttctcgtcct cgccgatgga accgtgatcg aaggcaaggg cctgggcgcg accggcgcgg ttgaggccga agtcgtcttc aacaccgctc tcaccggcta tgaggaaata ctgaccgacc cgtcctatgc cggacagatc gtcaccttca ctttcccaca tatcggcaat gtcggcgcca atgccgaaga tatcgaagat ctgacacccg ccaaccgcca cggcgcagtc ggcgcgatct tcaaggcaga catcaccgca ccctcgaatt tccgtgccgc aaaagacctc gatagctggc tcaagcatcg cggaatcatt gcgcttgccg gcatcgacac ccgcgccctc accgccctca tccgcgagcg tggcgcgcaa aacgccgtta tcgcgcatga cccgaacgga aatttcgatc tcgacgccct gaaagcgcgc gccgccaact ggtgcggcct tgaaaatctc gatctcgcca aggatgtcac catcggccag agcctcgtct ggaaggaact gccatggaca ctccaggacg gctatggcga acaggacgca ccgcaatatc acgtcgtcgc tctcgatttc ggtgtgaagc gcaatattct gcgccttctg accgggcttg gcgcaaaggt cacggtttta ccggccacgg caacagccga agacgttctg gcgcataatc ccgatggcgt tttcctgtcc aatggtcccg gcgatccggc ggcaacgggc gaatatgccg tgccgaccat cggcaagctg gtggaaaccg gcattcccct tttcggcatc tgcctcggcc accagatgct ggcgctggcg ctcggcggac gcacggaaaa gatgcatcag ggccatcacg gtgcaaacca cccggtgaag gattatacga ccggcaaggt ggaaatcgtc tcgatgaatc acggtttcgc agttgattcg gactcgctgc cggaaaatgt cgaggaaacc catgtgtcgc tcttcgacgg caccaattgc ggcctgcgtg tcgtcggcaa gccagtcttc tccgtgcagc atcacccgga agcctccccc ggcccgcagg attcgcacta tctgttccgc cggttcatca atctcatccg cgagaggaaa ggccaggctc ctctgccgga gcgcgaacag gctgcctga
Details for BrmeA.00204.a.A1.GE33695
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MTAQAGDANE TGVHTMTETT PKTAPWTVQK RTAVLVLADG TVIEGKGLGA TGAVEAEVVF NTALTGYEEI LTDPSYAGQI VTFTFPHIGN VGANAEDIED LTPANRHGAV GAIFKADITA PSNFRAAKDL DSWLKHRGII ALAGIDTRAL TALIRERGAQ NAVIAHDPNG NFDLDALKAR AANWCGLENL DLAKDVTIGQ SLVWKELPWT LQDGYGEQDA PQYHVVALDF GVKRNILRLL TGLGAKVTVL PATATAEDVL AHNPDGVFLS NGPGDPAATG EYAVPTIGKL VETGIPLFGI CLGHQMLALA LGGRTEKMHQ GHHGANHPVK DYTTGKVEIV SMNHGFAVDS DSLPENVEET HVSLFDGTNC GLRVVGKPVF SVQHHPEASP GPQDSHYLFR RFINLIR
Validated NT Sequence
agcggatgag attgatgaac cggcggaaca gatagtgcga atcctgcggg ccgggggagg cttccgggtg atgctgcacg gagaagactg gcttgccgac gacacgcagg ccgcaattgg tgccgtcgaa gagcgacaca tgggtttcct cgacattttc cggcagcgag tccgaatcaa ctgcgaaacc gtgattcatc gagacgattt ccaccttgcc ggtcgtataa tccttcaccg ggtggtttgc accgtgatgg ccctgatgca tcttttccgt gcgtccgccg agcgccagcg ccagcatctg gtggccgagg cagatgccga aaaggggaat gccggtttcc accagcttgc cgatggtcgg cacggcatat tcgcccgttg ccgccggatc gccgggacca ttggacagga aaacgccatc gggattatgc gccagaacgt cttcggctgt tgccgtggcc ggtaaaaccg tgacctttgc gccaagcccg gtcagaaggc gcagaatatt gcgcttcaca ccgaaatcga gagcgacgac gtgatattgc ggtgcgtcct gttcgccata gccgtcctgg agtgtccatg gcagttcctt ccagacgagg ctctggccga tggtgacatc cttggcgaga tcgagatttt caaggccgca ccagttggcg gcgcgcgctt tcagggcgtc gagatcgaaa tttccgttcg ggtcatgcgc gataacggcg ttttgcgcgc cacgctcgcg gatgagggcg gtgagggcgc gggtgtcgat gccggcaagc gcaatgattc cgcgatgctt gagccagcta tcgaggtctt ttgcggcacg gaaattcgag ggtgcggtga tgtctgcctt gaagatcgcg ccgactgcgc cgtggcggtt ggcgggtgtc agatcttcga tatcttcggc attggcgccg acattgccga tatgtgggaa agtgaaggtg acgatctgtc cggcatagga cgggtcggtc agtatttcct catagccggt gagagcggtg ttgaagacga cttcggcctc aaccgcgccg gtcgcgccca ggcccttgcc ttcgatcacg gttccatcgg cgaggacgag aacggcggtt cgcttctgaa cggtccaggg tgctgttttc ggggtcgttt cagtcatagt gtgcactcct gtttcgttgg catcgccagc ctgtgctgtc atcgaaccag gaccctgggt ctgagcttcc agggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAQAGDAN ETGVHTMTET TPKTAPWTVQ KRTAVLVLAD GTVIEGKGLG ATGAVEAEVV FNTALTGYEE ILTDPSYAGQ IVTFTFPHIG NVGANAEDIE DLTPANRHGA VGAIFKADIT APSNFRAAKD LDSWLKHRGI IALAGIDTRA LTALIRERGA QNAVIAHDPN GNFDLDALKA RAANWCGLEN LDLAKDVTIG QSLVWKELPW TLQDGYGEQD APQYHVVALD FGVKRNILRL LTGLGAKVTV LPATATAEDV LAHNPDGVFL SNGPGDPAAT GEYAVPTIGK LVETGIPLFG ICLGHQMLAL ALGGRTEKMH QGHHGANHPV KDYTTGKVEI VSMNHGFAVD SDSLPENVEE THVSLFDGTN CGLRVVGKPV FSVQHHPEAS PGPQDSHYLF RRFINLIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagcacag gctggcgatg ccaacgaaac aggagtgcac actatgactg aaacgacccc gaaaacagca ccctggaccg ttcagaagcg aaccgccgtt ctcgtcctcg ccgatggaac cgtgatcgaa ggcaagggcc tgggcgcgac cggcgcggtt gaggccgaag tcgtcttcaa caccgctctc accggctatg aggaaatact gaccgacccg tcctatgccg gacagatcgt caccttcact ttcccacata tcggcaatgt cggcgccaat gccgaagata tcgaagatct gacacccgcc aaccgccacg gcgcagtcgg cgcgatcttc aaggcagaca tcaccgcacc ctcgaatttc cgtgccgcaa aagacctcga tagctggctc aagcatcgcg gaatcattgc gcttgccggc atcgacaccc gcgccctcac cgccctcatc cgcgagcgtg gcgcgcaaaa cgccgttatc gcgcatgacc cgaacggaaa tttcgatctc gacgccctga aagcgcgcgc cgccaactgg tgcggccttg aaaatctcga tctcgccaag gatgtcacca tcggccagag cctcgtctgg aaggaactgc catggacact ccaggacggc tatggcgaac aggacgcacc gcaatatcac gtcgtcgctc tcgatttcgg tgtgaagcgc aatattctgc gccttctgac cgggcttggc gcaaaggtca cggttttacc ggccacggca acagccgaag acgttctggc gcataatccc gatggcgttt tcctgtccaa tggtcccggc gatccggcgg caacgggcga atatgccgtg ccgaccatcg gcaagctggt ggaaaccggc attccccttt tcggcatctg cctcggccac cagatgctgg cgctggcgct cggcggacgc acggaaaaga tgcatcaggg ccatcacggt gcaaaccacc cggtgaagga ttatacgacc ggcaaggtgg aaatcgtctc gatgaatcac ggtttcgcag ttgattcgga ctcgctgccg gaaaatgtcg aggaaaccca tgtgtcgctc ttcgacggca ccaattgcgg cctgcgtgtc gtcggcaagc cagtcttctc cgtgcagcat cacccggaag cctcccccgg cccgcaggat tcgcactatc tgttccgccg gttcatcaat ctcatccgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrmeA.00204.a.A1.PS01394
PURIFICATION DATe: 2/22/2012
CONCENTRATION: 13.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MTAQAGDANE TGVHTMTETT PKTAPWTVQK RTAVLVLADG TVIEGKGLGA TGAVEAEVVF NTALTGYEEI LTDPSYAGQI VTFTFPHIGN VGANAEDIED LTPANRHGAV GAIFKADITA PSNFRAAKDL DSWLKHRGII ALAGIDTRAL TALIRERGAQ NAVIAHDPNG NFDLDALKAR AANWCGLENL DLAKDVTIGQ SLVWKELPWT LQDGYGEQDA PQYHVVALDF GVKRNILRLL TGLGAKVTVL PATATAEDVL AHNPDGVFLS NGPGDPAATG EYAVPTIGKL VETGIPLFGI CLGHQMLALA LGGRTEKMHQ GHHGANHPVK DYTTGKVEIV SMNHGFAVDS DSLPENVEET HVSLFDGTNC GLRVVGKPVF SVQHHPEASP GPQDSHYLFR RFINLIR
Validated NT Sequence
agcggatgag attgatgaac cggcggaaca gatagtgcga atcctgcggg ccgggggagg cttccgggtg atgctgcacg gagaagactg gcttgccgac gacacgcagg ccgcaattgg tgccgtcgaa gagcgacaca tgggtttcct cgacattttc cggcagcgag tccgaatcaa ctgcgaaacc gtgattcatc gagacgattt ccaccttgcc ggtcgtataa tccttcaccg ggtggtttgc accgtgatgg ccctgatgca tcttttccgt gcgtccgccg agcgccagcg ccagcatctg gtggccgagg cagatgccga aaaggggaat gccggtttcc accagcttgc cgatggtcgg cacggcatat tcgcccgttg ccgccggatc gccgggacca ttggacagga aaacgccatc gggattatgc gccagaacgt cttcggctgt tgccgtggcc ggtaaaaccg tgacctttgc gccaagcccg gtcagaaggc gcagaatatt gcgcttcaca ccgaaatcga gagcgacgac gtgatattgc ggtgcgtcct gttcgccata gccgtcctgg agtgtccatg gcagttcctt ccagacgagg ctctggccga tggtgacatc cttggcgaga tcgagatttt caaggccgca ccagttggcg gcgcgcgctt tcagggcgtc gagatcgaaa tttccgttcg ggtcatgcgc gataacggcg ttttgcgcgc cacgctcgcg gatgagggcg gtgagggcgc gggtgtcgat gccggcaagc gcaatgattc cgcgatgctt gagccagcta tcgaggtctt ttgcggcacg gaaattcgag ggtgcggtga tgtctgcctt gaagatcgcg ccgactgcgc cgtggcggtt ggcgggtgtc agatcttcga tatcttcggc attggcgccg acattgccga tatgtgggaa agtgaaggtg acgatctgtc cggcatagga cgggtcggtc agtatttcct catagccggt gagagcggtg ttgaagacga cttcggcctc aaccgcgccg gtcgcgccca ggcccttgcc ttcgatcacg gttccatcgg cgaggacgag aacggcggtt cgcttctgaa cggtccaggg tgctgttttc ggggtcgttt cagtcatagt gtgcactcct gtttcgttgg catcgccagc ctgtgctgtc atcgaaccag gaccctgggt ctgagcttcc agggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAQAGDAN ETGVHTMTET TPKTAPWTVQ KRTAVLVLAD GTVIEGKGLG ATGAVEAEVV FNTALTGYEE ILTDPSYAGQ IVTFTFPHIG NVGANAEDIE DLTPANRHGA VGAIFKADIT APSNFRAAKD LDSWLKHRGI IALAGIDTRA LTALIRERGA QNAVIAHDPN GNFDLDALKA RAANWCGLEN LDLAKDVTIG QSLVWKELPW TLQDGYGEQD APQYHVVALD FGVKRNILRL LTGLGAKVTV LPATATAEDV LAHNPDGVFL SNGPGDPAAT GEYAVPTIGK LVETGIPLFG ICLGHQMLAL ALGGRTEKMH QGHHGANHPV KDYTTGKVEI VSMNHGFAVD SDSLPENVEE THVSLFDGTN CGLRVVGKPV FSVQHHPEAS PGPQDSHYLF RRFINLIR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGCACAG GCTGGCGATG CCAACGAAAC AGGAGTGCAC ACTATGACTG AAACGACCCC GAAAACAGCA CCCTGGACCG TTCAGAAGCG AACCGCCGTT CTCGTCCTCG CCGATGGAAC CGTGATCGAA GGCAAGGGCC TGGGCGCGAC CGGCGCGGTT GAGGCCGAAG TCGTCTTCAA CACCGCTCTC ACCGGCTATG AGGAAATACT GACCGACCCG TCCTATGCCG GACAGATCGT CACCTTCACT TTCCCACATA TCGGCAATGT CGGCGCCAAT GCCGAAGATA TCGAAGATCT GACACCCGCC AACCGCCACG GCGCAGTCGG CGCGATCTTC AAGGCAGACA TCACCGCACC CTCGAATTTC CGTGCCGCAA AAGACCTCGA TAGCTGGCTC AAGCATCGCG GAATCATTGC GCTTGCCGGC ATCGACACCC GCGCCCTCAC CGCCCTCATC CGCGAGCGTG GCGCGCAAAA CGCCGTTATC GCGCATGACC CGAACGGAAA TTTCGATCTC GACGCCCTGA AAGCGCGCGC CGCCAACTGG TGCGGCCTTG AAAATCTCGA TCTCGCCAAG GATGTCACCA TCGGCCAGAG CCTCGTCTGG AAGGAACTGC CATGGACACT CCAGGACGGC TATGGCGAAC AGGACGCACC GCAATATCAC GTCGTCGCTC TCGATTTCGG TGTGAAGCGC AATATTCTGC GCCTTCTGAC CGGGCTTGGC GCAAAGGTCA CGGTTTTACC GGCCACGGCA ACAGCCGAAG ACGTTCTGGC GCATAATCCC GATGGCGTTT TCCTGTCCAA TGGTCCCGGC GATCCGGCGG CAACGGGCGA ATATGCCGTG CCGACCATCG GCAAGCTGGT GGAAACCGGC ATTCCCCTTT TCGGCATCTG CCTCGGCCAC CAGATGCTGG CGCTGGCGCT CGGCGGACGC ACGGAAAAGA TGCATCAGGG CCATCACGGT GCAAACCACC CGGTGAAGGA TTATACGACC GGCAAGGTGG AAATCGTCTC GATGAATCAC GGTTTCGCAG TTGATTCGGA CTCGCTGCCG GAAAATGTCG AGGAAACCCA TGTGTCGCTC TTCGACGGCA CCAATTGCGG CCTGCGTGTC GTCGGCAAGC CAGTCTTCTC CGTGCAGCAT CACCCGGAAG CCTCCCCCGG CCCGCAGGAT TCGCACTATC TGTTCCGCCG GTTCATCAAT CTCATCCGCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrmeA.00204.a.A1.PS01393
PURIFICATION DATe: 2/22/2012
CONCENTRATION: 12.33mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MTAQAGDANE TGVHTMTETT PKTAPWTVQK RTAVLVLADG TVIEGKGLGA TGAVEAEVVF NTALTGYEEI LTDPSYAGQI VTFTFPHIGN VGANAEDIED LTPANRHGAV GAIFKADITA PSNFRAAKDL DSWLKHRGII ALAGIDTRAL TALIRERGAQ NAVIAHDPNG NFDLDALKAR AANWCGLENL DLAKDVTIGQ SLVWKELPWT LQDGYGEQDA PQYHVVALDF GVKRNILRLL TGLGAKVTVL PATATAEDVL AHNPDGVFLS NGPGDPAATG EYAVPTIGKL VETGIPLFGI CLGHQMLALA LGGRTEKMHQ GHHGANHPVK DYTTGKVEIV SMNHGFAVDS DSLPENVEET HVSLFDGTNC GLRVVGKPVF SVQHHPEASP GPQDSHYLFR RFINLIR
Validated NT Sequence
agcggatgag attgatgaac cggcggaaca gatagtgcga atcctgcggg ccgggggagg cttccgggtg atgctgcacg gagaagactg gcttgccgac gacacgcagg ccgcaattgg tgccgtcgaa gagcgacaca tgggtttcct cgacattttc cggcagcgag tccgaatcaa ctgcgaaacc gtgattcatc gagacgattt ccaccttgcc ggtcgtataa tccttcaccg ggtggtttgc accgtgatgg ccctgatgca tcttttccgt gcgtccgccg agcgccagcg ccagcatctg gtggccgagg cagatgccga aaaggggaat gccggtttcc accagcttgc cgatggtcgg cacggcatat tcgcccgttg ccgccggatc gccgggacca ttggacagga aaacgccatc gggattatgc gccagaacgt cttcggctgt tgccgtggcc ggtaaaaccg tgacctttgc gccaagcccg gtcagaaggc gcagaatatt gcgcttcaca ccgaaatcga gagcgacgac gtgatattgc ggtgcgtcct gttcgccata gccgtcctgg agtgtccatg gcagttcctt ccagacgagg ctctggccga tggtgacatc cttggcgaga tcgagatttt caaggccgca ccagttggcg gcgcgcgctt tcagggcgtc gagatcgaaa tttccgttcg ggtcatgcgc gataacggcg ttttgcgcgc cacgctcgcg gatgagggcg gtgagggcgc gggtgtcgat gccggcaagc gcaatgattc cgcgatgctt gagccagcta tcgaggtctt ttgcggcacg gaaattcgag ggtgcggtga tgtctgcctt gaagatcgcg ccgactgcgc cgtggcggtt ggcgggtgtc agatcttcga tatcttcggc attggcgccg acattgccga tatgtgggaa agtgaaggtg acgatctgtc cggcatagga cgggtcggtc agtatttcct catagccggt gagagcggtg ttgaagacga cttcggcctc aaccgcgccg gtcgcgccca ggcccttgcc ttcgatcacg gttccatcgg cgaggacgag aacggcggtt cgcttctgaa cggtccaggg tgctgttttc ggggtcgttt cagtcatagt gtgcactcct gtttcgttgg catcgccagc ctgtgctgtc atcgaaccag gaccctgggt ctgagcttcc agggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAQAGDAN ETGVHTMTET TPKTAPWTVQ KRTAVLVLAD GTVIEGKGLG ATGAVEAEVV FNTALTGYEE ILTDPSYAGQ IVTFTFPHIG NVGANAEDIE DLTPANRHGA VGAIFKADIT APSNFRAAKD LDSWLKHRGI IALAGIDTRA LTALIRERGA QNAVIAHDPN GNFDLDALKA RAANWCGLEN LDLAKDVTIG QSLVWKELPW TLQDGYGEQD APQYHVVALD FGVKRNILRL LTGLGAKVTV LPATATAEDV LAHNPDGVFL SNGPGDPAAT GEYAVPTIGK LVETGIPLFG ICLGHQMLAL ALGGRTEKMH QGHHGANHPV KDYTTGKVEI VSMNHGFAVD SDSLPENVEE THVSLFDGTN CGLRVVGKPV FSVQHHPEAS PGPQDSHYLF RRFINLIR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGCACAG GCTGGCGATG CCAACGAAAC AGGAGTGCAC ACTATGACTG AAACGACCCC GAAAACAGCA CCCTGGACCG TTCAGAAGCG AACCGCCGTT CTCGTCCTCG CCGATGGAAC CGTGATCGAA GGCAAGGGCC TGGGCGCGAC CGGCGCGGTT GAGGCCGAAG TCGTCTTCAA CACCGCTCTC ACCGGCTATG AGGAAATACT GACCGACCCG TCCTATGCCG GACAGATCGT CACCTTCACT TTCCCACATA TCGGCAATGT CGGCGCCAAT GCCGAAGATA TCGAAGATCT GACACCCGCC AACCGCCACG GCGCAGTCGG CGCGATCTTC AAGGCAGACA TCACCGCACC CTCGAATTTC CGTGCCGCAA AAGACCTCGA TAGCTGGCTC AAGCATCGCG GAATCATTGC GCTTGCCGGC ATCGACACCC GCGCCCTCAC CGCCCTCATC CGCGAGCGTG GCGCGCAAAA CGCCGTTATC GCGCATGACC CGAACGGAAA TTTCGATCTC GACGCCCTGA AAGCGCGCGC CGCCAACTGG TGCGGCCTTG AAAATCTCGA TCTCGCCAAG GATGTCACCA TCGGCCAGAG CCTCGTCTGG AAGGAACTGC CATGGACACT CCAGGACGGC TATGGCGAAC AGGACGCACC GCAATATCAC GTCGTCGCTC TCGATTTCGG TGTGAAGCGC AATATTCTGC GCCTTCTGAC CGGGCTTGGC GCAAAGGTCA CGGTTTTACC GGCCACGGCA ACAGCCGAAG ACGTTCTGGC GCATAATCCC GATGGCGTTT TCCTGTCCAA TGGTCCCGGC GATCCGGCGG CAACGGGCGA ATATGCCGTG CCGACCATCG GCAAGCTGGT GGAAACCGGC ATTCCCCTTT TCGGCATCTG CCTCGGCCAC CAGATGCTGG CGCTGGCGCT CGGCGGACGC ACGGAAAAGA TGCATCAGGG CCATCACGGT GCAAACCACC CGGTGAAGGA TTATACGACC GGCAAGGTGG AAATCGTCTC GATGAATCAC GGTTTCGCAG TTGATTCGGA CTCGCTGCCG GAAAATGTCG AGGAAACCCA TGTGTCGCTC TTCGACGGCA CCAATTGCGG CCTGCGTGTC GTCGGCAAGC CAGTCTTCTC CGTGCAGCAT CACCCGGAAG CCTCCCCCGG CCCGCAGGAT TCGCACTATC TGTTCCGCCG GTTCATCAAT CTCATCCGCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA