BrmeA.00183.a

Aspartate carbamoyltransferase (EC 2.1.3.2) (Aspartate transcarbamylase) (ATCase)

CENTER ID: BrmeA.00183.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00183.a.A1.GE33697 full length 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.2999
RefSeq: NP_541648.1
UniProt: P65611

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNQTVSPLF PHRHLLGIKG LSPLDILCLL DLADQEIAVS RQPEKKKSVL RGRTQINLFF EASTRTQSSF ELAGKRLGAD VMNMSVGNSS VKKGETLIDT AMTLNAMQPD ILVIRHASAG AAALLAQKVG CSVVNAGDGA HEHPTQALLD ALTIRRAKGQ IENLIVAICG DVLHSRVARS NILLLNALGA RVRVVAPSTL LPAGMADMSV EVFNSMEEGL KDADVVMMLR LQRERMAGSF VPSVREYFHF YGLDREKLKF AKPDALVMHP GPMNRGVEIA SDVADGPQSV IQQQVEMGVA VRMAVMEALL DPRRNPGNGE PA
NT Sequence
atgacaaatc aaacggtctc tccgcttttc cctcaccgcc acctgcttgg catcaagggg ctatcgcccc tggacattct ctgccttctc gatcttgccg atcaggaaat cgctgtctcc cggcagccgg agaagaaaaa gtccgtgctg cgcggtcgca cgcaaatcaa cctgttcttt gaagcatcga cacggacgca atcttcgttc gagctggccg gaaaacggct gggcgcggat gtgatgaata tgtccgtcgg caattcatcg gtcaagaaag gcgaaacgct catcgatacg gcgatgacgc tgaacgccat gcagccggat attctcgtca tccgccatgc ttccgccggt gctgccgccc ttctcgcgca gaaagtgggt tgctcggtgg tcaatgcggg cgacggcgcg catgaacacc ccacacaggc gcttctcgat gcgctgacaa tccgccgcgc caagggccag atcgaaaatc tgatcgtcgc catctgcggt gacgttctgc attcgcgcgt ggcgcgctcc aacattctcc tgctcaacgc gctcggcgcg cgggtgcggg tggttgcgcc ttcaacactc ctgcccgccg gcatggccga tatgagcgtg gaagttttca actccatgga ggaggggctg aaagacgctg atgtcgtcat gatgctgcgc ctccagcgcg agcgcatggc gggttccttc gtgccttccg tgcgggaata tttccatttc tatggcctgg accgggaaaa actcaaattc gccaaacccg atgcacttgt gatgcatccg gggccaatga accggggcgt cgagattgcc tccgacgttg cggacgggcc gcaaagcgtc atccagcaac aggtggaaat gggcgttgcc gtgcgcatgg ccgtgatgga ggctctgctc gatccgcggc gcaacccagg caacggagaa ccggcatga
Details for BrmeA.00183.a.A1.GE33697
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTNQTVSPL FPHRHLLGIK GLSPLDILCL LDLADQEIAV SRQPEKKKSV LRGRTQINLF FEASTRTQSS FELAGKRLGA DVMNMSVGNS SVKKGETLID TAMTLNAMQP DILVIRHASA GAAALLAQKV GCSVVNAGDG AHEHPTQALL DALTIRRAKG QIENLIVAIC GDVLHSRVAR SNILLLNALG ARVRVVAPST LLPAGMADMS VEVFNSMEEG LKDADVVMML RLXRERMAGS FVPSVREYFH FYGLDREKLK FAKPDALVMH XXANEPGRXD CLXRCGRAAK RHPATGXNGR CRAHGXDGXL LDPRXXXXXX XXKQHXXXXX XXXXXXXXXX XXXAXXXXXX XXX
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgacaaatc aaacggtctc tccgcttttc cctcaccgcc acctgcttgg catcaagggg ctatcgcccc tggacattct ctgccttctc gatcttgccg atcaggaaat cgctgtctcc cggcagccgg agaagaaaaa gtccgtgctg cgcggtcgca cgcaaatcaa cctgttcttt gaagcatcga cacggacgca atcttcgttc gagctggccg gaaaacggct gggcgcggat gtgatgaata tgtccgtcgg caattcatcg gtcaagaaag gcgaaacgct catcgatacg gcgatgacgc tgaacgccat gcagccggat attctcgtca tccgccatgc ttccgccggt gctgccgccc ttctcgcgca gaaagtgggt tgctcggtgg tcaatgcggg cgacggcgcg catgaacacc ccacacaggc gcttctcgat gcgctgacaa tccgccgcgc caagggccag atcgaaaatc tgatcgtcgc catctgcggt gacgttctgc attcgcgcgt ggcgcgctcc aacattctcc tgctcaacgc gctcggcgcg cgggtgcggg tggttgcgcc ttcaacactc ctgcccgccg gcatggccga tatgagcgtg gaagttttca actccatgga ggaggggctg aaagacgctg atgtcgtcat gatgctgcgc ctccngcgcg agcgcatggc gggttccttc gtgccttccg tgcgggaata tttccatttc tatggcctgg accgggaaaa actcaaattc gccaaacccg atgcacttgt gatgcatncn ngggccaatg aaccggggcg tcnagattgc ctccnacgtt gcggacgggc cgcaaagcgt catccagcaa cnggtgnaaa tgggcgttgc cgtgcgcatg gnngtgatgg nnntctgctc gatccgcgnn nnnccnnnnn nnannnnnnn nataaacagc acgannnnng cannnnctnn cnnngntnnn ncngctanna nnnnnnnnnc tnnnnngcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNQTVSPL FPHRHLLGIK GLSPLDILCL LDLADQEIAV SRQPEKKKSV LRGRTQINLF FEASTRTQSS FELAGKRLGA DVMNMSVGNS SVKKGETLID TAMTLNAMQP DILVIRHASA GAAALLAQKV GCSVVNAGDG AHEHPTQALL DALTIRRAKG QIENLIVAIC GDVLHSRVAR SNILLLNALG ARVRVVAPST LLPAGMADMS VEVFNSMEEG LKDADVVMML RLQRERMAGS FVPSVREYFH FYGLDREKLK FAKPDALVMH PGPMNRGVEI ASDVADGPQS VIQQQVEMGV AVRMAVMEAL LDPRRNPGNG EPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaatcaa acggtctctc cgcttttccc tcaccgccac ctgcttggca tcaaggggct atcgcccctg gacattctct gccttctcga tcttgccgat caggaaatcg ctgtctcccg gcagccggag aagaaaaagt ccgtgctgcg cggtcgcacg caaatcaacc tgttctttga agcatcgaca cggacgcaat cttcgttcga gctggccgga aaacggctgg gcgcggatgt gatgaatatg tccgtcggca attcatcggt caagaaaggc gaaacgctca tcgatacggc gatgacgctg aacgccatgc agccggatat tctcgtcatc cgccatgctt ccgccggtgc tgccgccctt ctcgcgcaga aagtgggttg ctcggtggtc aatgcgggcg acggcgcgca tgaacacccc acacaggcgc ttctcgatgc gctgacaatc cgccgcgcca agggccagat cgaaaatctg atcgtcgcca tctgcggtga cgttctgcat tcgcgcgtgg cgcgctccaa cattctcctg ctcaacgcgc tcggcgcgcg ggtgcgggtg gttgcgcctt caacactcct gcccgccggc atggccgata tgagcgtgga agttttcaac tccatggagg aggggctgaa agacgctgat gtcgtcatga tgctgcgcct ccagcgcgag cgcatggcgg gttccttcgt gccttccgtg cgggaatatt tccatttcta tggcctggac cgggaaaaac tcaaattcgc caaacccgat gcacttgtga tgcatccggg gccaatgaac cggggcgtcg agattgcctc cgacgttgcg gacgggccgc aaagcgtcat ccagcaacag gtggaaatgg gcgttgccgt gcgcatggcc gtgatggagg ctctgctcga tccgcggcgc aacccaggca acggagaacc ggcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa