BrmeA.00143.d

2-keto-3-deoxy-L-fuconate dehydrogenase

CENTER ID:	BrmeA.00143.d
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00143.d.B1.GE36682	Full length( BrmeA.00143.d )		1	244

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|224914.11.peg.1891
RefSeq:	NP_540626.1
UniProt:	Q8YF18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIRLDGKTA LVTAAGQGIG QASALAFVEA GAKVYATDIN ADALAKLEGI AGIVTRRLDV LDDAAVKALV AEIGQIDILF NCAGFVHNGT ILEMKDEELD FAVNLNVRSM IRTIRAVLPG MLERKDGAII NMASVASSVK GVSNRFVYGL TKAAVIGLTK AVAADYVSKG IRCNAICPGT VESPSLQDRL RAQGDYEAAR AAFIARQPIG RIGQPEEIAD LAVYLAGATY TTGQAYNIDG GWSI

NT Sequence

ATGACGATAC GACTGGATGG AAAGACCGCG CTTGTGACGG CCGCTGGGCA AGGGATTGGG CAGGCAAGTG CGCTGGCCTT TGTAGAGGCG GGCGCCAAGG TCTATGCGAC CGACATCAAT GCCGATGCGC TTGCGAAACT GGAAGGCATA GCCGGCATTG TCACGCGCAG GCTGGACGTG CTGGACGACG CTGCGGTCAA GGCGCTGGTG GCTGAAATCG GGCAGATCGA TATCCTCTTC AACTGCGCCG GTTTTGTCCA CAACGGGACC ATCCTTGAGA TGAAGGACGA GGAACTGGAT TTTGCCGTCA ACCTGAATGT CCGCTCCATG ATCCGCACCA TCCGCGCCGT GCTGCCGGGA ATGCTGGAGC GCAAGGATGG CGCCATCATC AACATGGCTT CCGTCGCATC GAGCGTCAAA GGGGTGTCGA ATCGTTTTGT CTATGGTCTG ACCAAGGCGG CGGTAATCGG GCTCACCAAG GCGGTCGCCG CCGATTATGT GAGCAAGGGC ATCCGCTGCA ACGCCATTTG TCCCGGCACG GTGGAAAGCC CATCCTTGCA GGACCGCCTG CGGGCGCAGG GCGATTATGA GGCTGCCCGC GCTGCCTTCA TCGCCCGCCA GCCCATCGGC CGCATCGGCC AGCCGGAGGA AATCGCCGAC CTCGCAGTCT ATCTGGCAGG CGCGACCTAT ACCACCGGGC AGGCCTATAA CATCGATGGG GGATGGTCGA TC

Details for BrmeA.00143.d.B1.GE36682

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT IRLDGKTALV TAAGQGIGQA SALAFVEAGA KVYATDINAD ALAKLEGIAG IVTRRLDVLD DAAVKALVAE IGQIDILFNC AGFVHNGTIL EMKDEELDFA VNLNVRSMIR TIRAVLPGML ERKDGAIINM ASVASSVKGV SNRFVYGLTK AAVIGLTKAV AADYVSKGIR CNAICPGTVE SPSLQDRLRA QGDYEAARAA FIARQPIGRI GQPEEIADLA VYLAGATYTT GQAYNIDGGW SI

Validated NT Sequence

atcttactca cttagatcga ccatccccca tcgatgttat aggcctgccc ggtggtatag gtcgcgcctg ccagatagac tgcgaggtcg gcgatttcct ccggctggcc gatgcggccg atgggctggc gggcgatgaa ggcagcgcgg gcagcctcat aatcgccctg cgcccgcagg cggtcctgca aggatgggct ttccaccgtg ccgggacaaa tggcgttgca gcggatgccc ttgctcacat aatcggcggc gaccgccttg gtgagcccga ttaccgccgc cttggtcaga ccatagacaa aacgattcga cacccctttg acgctcgatg cgacggaagc catgttgatg atggcgccat ccttgcgctc cagcattccc ggcagcacgg cgcggatggt gcggatcatg gagcggacat tcaggttgac ggcaaaatcc agttcctcgt ccttcatctc aaggatggtc ccgttgtgga caaaaccggc gcagttgaag aggatatcga tctgcccgat ttcagccacc agcgccttga ccgcagcgtc gtccagcacg tccagcctgc gcgtgacaat gccggctatg ccttccagtt tcgcaagcgc atcggcattg atgtcggtcg catagacctt ggcgcccgcc tctacaaagg ccagcgcact tgcctgccca atcccttgcc cagcggccgt cacaagcgcg gtctttccat ccagtcgtat cgtcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga tacgactgga tggaaagacc gcgcttgtga cggccgctgg gcaagggatt gggcaggcaa gtgcgctggc ctttgtagag gcgggcgcca aggtctatgc gaccgacatc aatgccgatg cgcttgcgaa actggaaggc atagccggca ttgtcacgcg caggctggac gtgctggacg acgctgcggt caaggcgctg gtggctgaaa tcgggcagat cgatatcctc ttcaactgcg ccggttttgt ccacaacggg accatccttg agatgaagga cgaggaactg gattttgccg tcaacctgaa tgtccgctcc atgatccgca ccatccgcgc cgtgctgccg ggaatgctgg agcgcaagga tggcgccatc atcaacatgg cttccgtcgc atcgagcgtc aaaggggtgt cgaatcgttt tgtctatggt ctgaccaagg cggcggtaat cgggctcacc aaggcggtcg ccgccgatta tgtgagcaag ggcatccgct gcaacgccat ttgtcccggc acggtggaaa gcccatcctt gcaggaccgc ctgcgggcgc agggcgatta tgaggctgcc cgcgctgcct tcatcgcccg ccagcccatc ggccgcatcg gccagccgga ggaaatcgcc gacctcgcag tctatctggc aggcgcgacc tataccaccg ggcaggccta taacatcgat gggggatggt cgatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat